Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5770 and Pf6N2E2_5771 are separated by 269 nucleotides Pf6N2E2_5771 and Pf6N2E2_5772 are separated by 294 nucleotides Pf6N2E2_5772 and Pf6N2E2_5773 are separated by 90 nucleotides
Pf6N2E2_5770 - FIG00959155: hypothetical protein, at 6,586,109 to 6,587,914
_5770
Pf6N2E2_5771 - FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8), at 6,588,184 to 6,588,897
_5771
Pf6N2E2_5772 - DUF1232 domain-containing protein, at 6,589,192 to 6,589,647
_5772
Pf6N2E2_5773 - Transcriptional regulator, Cro/CI family, at 6,589,738 to 6,590,103
_5773
Position (kb)
6588
6589 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6587.319 kb on + strand, within Pf6N2E2_5770 at 6587.320 kb on - strand, within Pf6N2E2_5770 at 6587.320 kb on - strand, within Pf6N2E2_5770 at 6587.320 kb on - strand, within Pf6N2E2_5770 at 6587.370 kb on + strand, within Pf6N2E2_5770 at 6587.370 kb on + strand, within Pf6N2E2_5770 at 6587.370 kb on + strand, within Pf6N2E2_5770 at 6587.370 kb on + strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.371 kb on - strand, within Pf6N2E2_5770 at 6587.373 kb on + strand, within Pf6N2E2_5770 at 6587.373 kb on + strand, within Pf6N2E2_5770 at 6587.374 kb on - strand, within Pf6N2E2_5770 at 6587.374 kb on - strand, within Pf6N2E2_5770 at 6587.374 kb on - strand, within Pf6N2E2_5770 at 6587.374 kb on - strand, within Pf6N2E2_5770 at 6587.377 kb on + strand, within Pf6N2E2_5770 at 6587.378 kb on - strand, within Pf6N2E2_5770 at 6587.378 kb on - strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.428 kb on + strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.429 kb on - strand, within Pf6N2E2_5770 at 6587.653 kb on - strand, within Pf6N2E2_5770 at 6587.653 kb on - strand, within Pf6N2E2_5770 at 6587.751 kb on + strand at 6587.751 kb on + strand at 6587.751 kb on + strand at 6587.751 kb on + strand at 6587.751 kb on + strand at 6587.752 kb on - strand at 6587.752 kb on - strand at 6587.752 kb on - strand at 6587.752 kb on - strand at 6587.752 kb on - strand at 6587.754 kb on + strand at 6587.754 kb on + strand at 6587.754 kb on + strand at 6587.754 kb on + strand at 6587.755 kb on - strand at 6587.755 kb on - strand at 6587.755 kb on - strand at 6587.755 kb on - strand at 6587.755 kb on - strand at 6587.791 kb on - strand at 6587.791 kb on - strand at 6587.815 kb on - strand at 6587.841 kb on + strand at 6587.841 kb on + strand at 6587.841 kb on + strand at 6587.842 kb on - strand at 6587.842 kb on - strand at 6587.842 kb on - strand at 6587.844 kb on + strand at 6587.844 kb on + strand at 6587.844 kb on + strand at 6587.845 kb on - strand at 6587.845 kb on - strand at 6587.945 kb on + strand at 6587.945 kb on + strand at 6588.017 kb on + strand at 6588.018 kb on - strand at 6588.018 kb on - strand at 6588.021 kb on + strand at 6588.022 kb on - strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.079 kb on + strand at 6588.080 kb on - strand at 6588.131 kb on + strand at 6588.132 kb on - strand at 6588.180 kb on + strand at 6588.180 kb on + strand at 6588.180 kb on + strand at 6588.181 kb on - strand at 6588.181 kb on - strand at 6588.230 kb on + strand at 6588.231 kb on - strand at 6588.261 kb on - strand, within Pf6N2E2_5771 at 6588.540 kb on + strand, within Pf6N2E2_5771 at 6588.565 kb on + strand, within Pf6N2E2_5771 at 6588.614 kb on + strand, within Pf6N2E2_5771 at 6588.614 kb on + strand, within Pf6N2E2_5771 at 6588.628 kb on + strand, within Pf6N2E2_5771 at 6588.628 kb on + strand at 6588.629 kb on - strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.760 kb on + strand, within Pf6N2E2_5771 at 6588.761 kb on - strand, within Pf6N2E2_5771 at 6588.761 kb on - strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.769 kb on + strand, within Pf6N2E2_5771 at 6588.895 kb on + strand at 6588.895 kb on + strand at 6588.896 kb on - strand at 6588.896 kb on - strand at 6588.896 kb on - strand at 6588.908 kb on + strand at 6588.908 kb on + strand at 6588.908 kb on + strand at 6588.909 kb on - strand at 6588.909 kb on - strand at 6588.909 kb on - strand at 6588.909 kb on - strand at 6588.909 kb on - strand at 6588.995 kb on - strand at 6588.995 kb on - strand at 6588.995 kb on - strand at 6588.995 kb on - strand at 6588.995 kb on - strand at 6588.996 kb on + strand at 6588.996 kb on + strand at 6588.996 kb on + strand at 6588.996 kb on + strand at 6588.996 kb on + strand at 6588.997 kb on - strand at 6588.997 kb on - strand at 6588.997 kb on - strand at 6588.997 kb on - strand at 6589.003 kb on - strand at 6589.003 kb on - strand at 6589.003 kb on - strand at 6589.003 kb on - strand at 6589.003 kb on - strand at 6589.009 kb on + strand at 6589.010 kb on - strand at 6589.023 kb on + strand at 6589.054 kb on + strand at 6589.067 kb on + strand at 6589.104 kb on + strand at 6589.151 kb on + strand at 6589.363 kb on + strand, within Pf6N2E2_5772 at 6589.429 kb on + strand, within Pf6N2E2_5772 at 6589.429 kb on + strand, within Pf6N2E2_5772 at 6589.429 kb on + strand, within Pf6N2E2_5772 at 6589.430 kb on - strand, within Pf6N2E2_5772 at 6589.430 kb on - strand, within Pf6N2E2_5772 at 6589.461 kb on + strand, within Pf6N2E2_5772 at 6589.461 kb on + strand, within Pf6N2E2_5772 at 6589.462 kb on - strand, within Pf6N2E2_5772 at 6589.700 kb on + strand at 6589.700 kb on - strand at 6589.701 kb on - strand at 6589.701 kb on - strand at 6589.706 kb on + strand at 6589.714 kb on + strand at 6589.715 kb on - strand at 6589.735 kb on + strand at 6589.757 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,587,319 + Pf6N2E2_5770 0.67 +0.2 6,587,320 - Pf6N2E2_5770 0.67 +0.6 6,587,320 - Pf6N2E2_5770 0.67 +0.2 6,587,320 - Pf6N2E2_5770 0.67 +1.8 6,587,370 + Pf6N2E2_5770 0.70 +1.8 6,587,370 + Pf6N2E2_5770 0.70 +0.2 6,587,370 + Pf6N2E2_5770 0.70 +0.8 6,587,370 + Pf6N2E2_5770 0.70 -0.5 6,587,371 - Pf6N2E2_5770 0.70 -1.5 6,587,371 - Pf6N2E2_5770 0.70 +1.3 6,587,371 - Pf6N2E2_5770 0.70 -0.7 6,587,371 - Pf6N2E2_5770 0.70 +0.5 6,587,371 - Pf6N2E2_5770 0.70 +0.3 6,587,371 - Pf6N2E2_5770 0.70 +0.4 6,587,373 + Pf6N2E2_5770 0.70 -0.3 6,587,373 + Pf6N2E2_5770 0.70 +0.4 6,587,374 - Pf6N2E2_5770 0.70 -0.4 6,587,374 - Pf6N2E2_5770 0.70 -0.1 6,587,374 - Pf6N2E2_5770 0.70 +0.6 6,587,374 - Pf6N2E2_5770 0.70 +0.1 6,587,377 + Pf6N2E2_5770 0.70 +0.5 6,587,378 - Pf6N2E2_5770 0.70 +0.1 6,587,378 - Pf6N2E2_5770 0.70 +0.7 6,587,428 + Pf6N2E2_5770 0.73 -0.2 6,587,428 + Pf6N2E2_5770 0.73 +0.3 6,587,428 + Pf6N2E2_5770 0.73 +2.0 6,587,428 + Pf6N2E2_5770 0.73 +0.2 6,587,428 + Pf6N2E2_5770 0.73 -0.4 6,587,428 + Pf6N2E2_5770 0.73 -0.0 6,587,429 - Pf6N2E2_5770 0.73 +0.5 6,587,429 - Pf6N2E2_5770 0.73 -0.0 6,587,429 - Pf6N2E2_5770 0.73 +1.0 6,587,429 - Pf6N2E2_5770 0.73 -1.2 6,587,429 - Pf6N2E2_5770 0.73 -0.3 6,587,653 - Pf6N2E2_5770 0.85 +0.0 6,587,653 - Pf6N2E2_5770 0.85 -0.8 6,587,751 + -1.3 6,587,751 + +0.2 6,587,751 + -0.7 6,587,751 + +0.3 6,587,751 + +0.2 6,587,752 - +1.0 6,587,752 - +0.8 6,587,752 - +1.0 6,587,752 - +1.0 6,587,752 - -0.2 6,587,754 + -0.0 6,587,754 + -1.0 6,587,754 + +2.5 6,587,754 + -0.9 6,587,755 - -0.5 6,587,755 - -0.0 6,587,755 - +1.2 6,587,755 - +1.2 6,587,755 - +1.3 6,587,791 - +0.1 6,587,791 - +0.6 6,587,815 - +0.1 6,587,841 + +0.5 6,587,841 + +1.0 6,587,841 + +0.1 6,587,842 - +0.7 6,587,842 - -1.1 6,587,842 - +1.1 6,587,844 + -0.5 6,587,844 + +0.3 6,587,844 + +0.4 6,587,845 - -0.1 6,587,845 - -1.6 6,587,945 + -0.5 6,587,945 + -0.4 6,588,017 + +0.2 6,588,018 - -0.2 6,588,018 - -0.9 6,588,021 + -0.1 6,588,022 - +0.1 6,588,079 + -0.5 6,588,079 + -0.7 6,588,079 + -0.1 6,588,079 + -0.9 6,588,079 + -0.2 6,588,079 + -0.7 6,588,080 - +0.9 6,588,131 + +0.5 6,588,132 - +0.5 6,588,180 + -1.9 6,588,180 + +0.1 6,588,180 + +2.3 6,588,181 - +1.1 6,588,181 - -0.6 6,588,230 + -0.3 6,588,231 - +2.3 6,588,261 - Pf6N2E2_5771 0.11 -0.6 6,588,540 + Pf6N2E2_5771 0.50 +0.2 6,588,565 + Pf6N2E2_5771 0.53 +1.1 6,588,614 + Pf6N2E2_5771 0.60 +1.1 6,588,614 + Pf6N2E2_5771 0.60 -1.0 6,588,628 + Pf6N2E2_5771 0.62 -0.5 6,588,628 + +0.5 6,588,629 - Pf6N2E2_5771 0.62 +1.1 6,588,760 + Pf6N2E2_5771 0.81 -0.9 6,588,760 + Pf6N2E2_5771 0.81 -1.8 6,588,760 + Pf6N2E2_5771 0.81 -1.1 6,588,760 + Pf6N2E2_5771 0.81 +0.9 6,588,760 + Pf6N2E2_5771 0.81 +0.5 6,588,760 + Pf6N2E2_5771 0.81 -1.0 6,588,761 - Pf6N2E2_5771 0.81 +0.4 6,588,761 - Pf6N2E2_5771 0.81 -1.2 6,588,769 + Pf6N2E2_5771 0.82 +0.1 6,588,769 + Pf6N2E2_5771 0.82 -0.1 6,588,769 + Pf6N2E2_5771 0.82 -0.9 6,588,769 + Pf6N2E2_5771 0.82 -1.5 6,588,769 + Pf6N2E2_5771 0.82 -1.2 6,588,769 + Pf6N2E2_5771 0.82 -0.7 6,588,895 + -0.6 6,588,895 + -0.2 6,588,896 - +0.7 6,588,896 - +0.6 6,588,896 - +0.5 6,588,908 + -0.8 6,588,908 + -0.3 6,588,908 + -0.6 6,588,909 - -0.1 6,588,909 - +1.3 6,588,909 - -0.1 6,588,909 - -1.3 6,588,909 - +0.5 6,588,995 - +0.8 6,588,995 - +0.2 6,588,995 - -0.6 6,588,995 - +0.1 6,588,995 - -0.0 6,588,996 + -0.6 6,588,996 + +0.7 6,588,996 + +0.7 6,588,996 + -2.6 6,588,996 + +0.1 6,588,997 - +0.3 6,588,997 - +0.2 6,588,997 - +1.0 6,588,997 - -1.9 6,589,003 - +0.5 6,589,003 - -1.1 6,589,003 - +0.5 6,589,003 - +1.2 6,589,003 - -0.3 6,589,009 + +0.1 6,589,010 - +1.0 6,589,023 + +0.8 6,589,054 + +0.4 6,589,067 + +0.7 6,589,104 + -1.0 6,589,151 + -0.7 6,589,363 + Pf6N2E2_5772 0.38 +0.6 6,589,429 + Pf6N2E2_5772 0.52 -1.4 6,589,429 + Pf6N2E2_5772 0.52 -0.9 6,589,429 + Pf6N2E2_5772 0.52 +0.5 6,589,430 - Pf6N2E2_5772 0.52 +0.3 6,589,430 - Pf6N2E2_5772 0.52 +0.5 6,589,461 + Pf6N2E2_5772 0.59 +0.2 6,589,461 + Pf6N2E2_5772 0.59 +0.8 6,589,462 - Pf6N2E2_5772 0.59 +1.1 6,589,700 + +0.5 6,589,700 - +0.7 6,589,701 - +1.3 6,589,701 - +1.5 6,589,706 + +0.9 6,589,714 + -1.1 6,589,715 - +1.2 6,589,735 + +0.2 6,589,757 + +0.4
Or see this region's nucleotide sequence