Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5731 and Pf6N2E2_5732 are separated by 169 nucleotides Pf6N2E2_5732 and Pf6N2E2_5733 are separated by 289 nucleotides
Pf6N2E2_5731 - Putative permease often clustered with de novo purine synthesis, at 6,548,169 to 6,549,242
_5731
Pf6N2E2_5732 - Chromosomal replication initiator protein DnaA, at 6,549,412 to 6,550,116
_5732
Pf6N2E2_5733 - NLP/P60 family protein, at 6,550,406 to 6,550,957
_5733
Position (kb)
6549
6550
6551 Strain fitness (log2 ratio)
-1
0
1
2
3 at 6548.497 kb on + strand, within Pf6N2E2_5731 at 6548.498 kb on - strand, within Pf6N2E2_5731 at 6548.498 kb on - strand, within Pf6N2E2_5731 at 6548.555 kb on + strand, within Pf6N2E2_5731 at 6548.555 kb on + strand, within Pf6N2E2_5731 at 6548.556 kb on - strand, within Pf6N2E2_5731 at 6548.556 kb on - strand, within Pf6N2E2_5731 at 6548.667 kb on + strand, within Pf6N2E2_5731 at 6548.667 kb on + strand, within Pf6N2E2_5731 at 6548.667 kb on + strand, within Pf6N2E2_5731 at 6548.667 kb on + strand, within Pf6N2E2_5731 at 6548.667 kb on + strand, within Pf6N2E2_5731 at 6548.668 kb on - strand, within Pf6N2E2_5731 at 6548.668 kb on - strand, within Pf6N2E2_5731 at 6548.668 kb on - strand, within Pf6N2E2_5731 at 6548.668 kb on - strand, within Pf6N2E2_5731 at 6548.826 kb on + strand, within Pf6N2E2_5731 at 6548.826 kb on + strand, within Pf6N2E2_5731 at 6548.826 kb on + strand, within Pf6N2E2_5731 at 6548.826 kb on + strand, within Pf6N2E2_5731 at 6548.826 kb on + strand, within Pf6N2E2_5731 at 6548.826 kb on + strand, within Pf6N2E2_5731 at 6548.826 kb on + strand, within Pf6N2E2_5731 at 6548.848 kb on + strand, within Pf6N2E2_5731 at 6548.907 kb on + strand, within Pf6N2E2_5731 at 6548.907 kb on + strand, within Pf6N2E2_5731 at 6548.907 kb on + strand, within Pf6N2E2_5731 at 6548.907 kb on + strand, within Pf6N2E2_5731 at 6548.907 kb on + strand, within Pf6N2E2_5731 at 6548.907 kb on + strand, within Pf6N2E2_5731 at 6548.907 kb on + strand, within Pf6N2E2_5731 at 6548.908 kb on - strand, within Pf6N2E2_5731 at 6548.908 kb on - strand, within Pf6N2E2_5731 at 6548.908 kb on - strand, within Pf6N2E2_5731 at 6548.927 kb on + strand, within Pf6N2E2_5731 at 6549.062 kb on + strand, within Pf6N2E2_5731 at 6549.062 kb on + strand, within Pf6N2E2_5731 at 6549.063 kb on - strand, within Pf6N2E2_5731 at 6549.182 kb on + strand at 6549.182 kb on + strand at 6549.183 kb on - strand at 6549.183 kb on - strand at 6549.195 kb on + strand at 6549.213 kb on + strand at 6549.213 kb on + strand at 6549.213 kb on + strand at 6549.213 kb on + strand at 6549.213 kb on + strand at 6549.213 kb on + strand at 6549.213 kb on + strand at 6549.352 kb on + strand at 6549.352 kb on + strand at 6549.352 kb on + strand at 6550.114 kb on + strand at 6550.115 kb on - strand at 6550.185 kb on - strand at 6550.220 kb on + strand at 6550.220 kb on + strand at 6550.226 kb on + strand at 6550.226 kb on + strand at 6550.226 kb on + strand at 6550.226 kb on + strand at 6550.227 kb on - strand at 6550.227 kb on - strand at 6550.282 kb on + strand at 6550.282 kb on + strand at 6550.282 kb on + strand at 6550.282 kb on + strand at 6550.283 kb on - strand at 6550.292 kb on - strand at 6550.306 kb on + strand at 6550.306 kb on + strand at 6550.306 kb on + strand at 6550.307 kb on - strand at 6550.309 kb on + strand at 6550.335 kb on + strand at 6550.336 kb on - strand at 6550.529 kb on + strand, within Pf6N2E2_5733 at 6550.529 kb on + strand, within Pf6N2E2_5733 at 6550.529 kb on + strand, within Pf6N2E2_5733 at 6550.805 kb on + strand, within Pf6N2E2_5733 at 6550.805 kb on + strand, within Pf6N2E2_5733 at 6550.805 kb on + strand, within Pf6N2E2_5733 at 6550.805 kb on + strand, within Pf6N2E2_5733 at 6550.883 kb on + strand, within Pf6N2E2_5733 at 6550.883 kb on + strand, within Pf6N2E2_5733 at 6550.955 kb on + strand at 6550.955 kb on + strand at 6550.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,548,497 + Pf6N2E2_5731 0.31 -0.2 6,548,498 - Pf6N2E2_5731 0.31 -0.1 6,548,498 - Pf6N2E2_5731 0.31 +2.3 6,548,555 + Pf6N2E2_5731 0.36 +0.2 6,548,555 + Pf6N2E2_5731 0.36 +0.3 6,548,556 - Pf6N2E2_5731 0.36 -0.0 6,548,556 - Pf6N2E2_5731 0.36 +0.2 6,548,667 + Pf6N2E2_5731 0.46 +0.6 6,548,667 + Pf6N2E2_5731 0.46 +0.8 6,548,667 + Pf6N2E2_5731 0.46 +0.1 6,548,667 + Pf6N2E2_5731 0.46 +0.5 6,548,667 + Pf6N2E2_5731 0.46 -1.2 6,548,668 - Pf6N2E2_5731 0.46 +1.0 6,548,668 - Pf6N2E2_5731 0.46 +0.1 6,548,668 - Pf6N2E2_5731 0.46 +0.4 6,548,668 - Pf6N2E2_5731 0.46 +1.5 6,548,826 + Pf6N2E2_5731 0.61 -1.3 6,548,826 + Pf6N2E2_5731 0.61 +1.0 6,548,826 + Pf6N2E2_5731 0.61 +1.4 6,548,826 + Pf6N2E2_5731 0.61 +0.5 6,548,826 + Pf6N2E2_5731 0.61 -0.0 6,548,826 + Pf6N2E2_5731 0.61 -0.1 6,548,826 + Pf6N2E2_5731 0.61 +0.7 6,548,848 + Pf6N2E2_5731 0.63 -0.1 6,548,907 + Pf6N2E2_5731 0.69 -0.1 6,548,907 + Pf6N2E2_5731 0.69 +0.4 6,548,907 + Pf6N2E2_5731 0.69 +2.3 6,548,907 + Pf6N2E2_5731 0.69 -0.3 6,548,907 + Pf6N2E2_5731 0.69 -0.4 6,548,907 + Pf6N2E2_5731 0.69 +0.3 6,548,907 + Pf6N2E2_5731 0.69 -0.0 6,548,908 - Pf6N2E2_5731 0.69 -0.6 6,548,908 - Pf6N2E2_5731 0.69 +0.4 6,548,908 - Pf6N2E2_5731 0.69 -0.3 6,548,927 + Pf6N2E2_5731 0.71 -0.8 6,549,062 + Pf6N2E2_5731 0.83 +0.7 6,549,062 + Pf6N2E2_5731 0.83 -0.2 6,549,063 - Pf6N2E2_5731 0.83 -0.8 6,549,182 + +0.3 6,549,182 + -0.4 6,549,183 - -0.2 6,549,183 - -1.0 6,549,195 + +0.5 6,549,213 + +1.8 6,549,213 + +0.8 6,549,213 + -1.1 6,549,213 + +0.2 6,549,213 + +0.5 6,549,213 + +0.8 6,549,213 + +0.4 6,549,352 + -0.0 6,549,352 + +0.6 6,549,352 + +1.5 6,550,114 + +0.2 6,550,115 - -1.1 6,550,185 - +2.9 6,550,220 + +1.0 6,550,220 + +0.5 6,550,226 + -1.0 6,550,226 + +0.5 6,550,226 + -1.5 6,550,226 + -0.1 6,550,227 - +0.7 6,550,227 - -1.0 6,550,282 + -0.6 6,550,282 + +0.2 6,550,282 + +1.5 6,550,282 + -0.0 6,550,283 - -0.0 6,550,292 - -0.6 6,550,306 + -0.2 6,550,306 + -1.5 6,550,306 + -0.2 6,550,307 - +1.2 6,550,309 + +1.1 6,550,335 + +1.0 6,550,336 - -0.0 6,550,529 + Pf6N2E2_5733 0.22 -0.5 6,550,529 + Pf6N2E2_5733 0.22 -1.6 6,550,529 + Pf6N2E2_5733 0.22 -0.1 6,550,805 + Pf6N2E2_5733 0.72 +0.6 6,550,805 + Pf6N2E2_5733 0.72 +1.1 6,550,805 + Pf6N2E2_5733 0.72 -0.3 6,550,805 + Pf6N2E2_5733 0.72 +0.4 6,550,883 + Pf6N2E2_5733 0.86 -0.3 6,550,883 + Pf6N2E2_5733 0.86 -0.6 6,550,955 + -0.3 6,550,955 + +0.6 6,550,956 - +0.4
Or see this region's nucleotide sequence