Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5677

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5676 and Pf6N2E2_5677 are separated by 186 nucleotidesPf6N2E2_5677 and Pf6N2E2_6158 are separated by 62 nucleotidesPf6N2E2_6158 and Pf6N2E2_6159 are separated by 111 nucleotidesPf6N2E2_6159 and Pf6N2E2_6160 are separated by 75 nucleotidesPf6N2E2_6160 and Pf6N2E2_5678 are separated by 43 nucleotides Pf6N2E2_5676 - Aspartokinase (EC 2.7.2.4), at 6,490,042 to 6,491,283 _5676 Pf6N2E2_5677 - Carbon storage regulator, at 6,491,470 to 6,491,658 _5677 Pf6N2E2_6158 - tRNA-Ser-GCT, at 6,491,721 to 6,491,808 _6158 Pf6N2E2_6159 - tRNA-Arg-ACG, at 6,491,920 to 6,491,993 _6159 Pf6N2E2_6160 - tRNA-Arg-ACG, at 6,492,069 to 6,492,142 _6160 Pf6N2E2_5678 - hypothetical protein, at 6,492,186 to 6,492,692 _5678 Position (kb) 6491 6492Strain fitness (log2 ratio) -2 -1 0 1 2at 6490.767 kb on + strand, within Pf6N2E2_5676at 6490.767 kb on + strand, within Pf6N2E2_5676at 6490.767 kb on + strand, within Pf6N2E2_5676at 6490.767 kb on + strand, within Pf6N2E2_5676at 6490.767 kb on + strand, within Pf6N2E2_5676at 6490.770 kb on + strand, within Pf6N2E2_5676at 6491.374 kb on + strandat 6491.374 kb on + strandat 6491.377 kb on + strandat 6491.377 kb on + strandat 6491.377 kb on + strandat 6491.377 kb on + strandat 6491.377 kb on + strandat 6491.407 kb on + strandat 6491.407 kb on + strandat 6491.407 kb on + strandat 6491.444 kb on + strandat 6491.444 kb on + strandat 6491.469 kb on + strandat 6491.469 kb on + strandat 6491.469 kb on + strandat 6491.508 kb on + strand, within Pf6N2E2_5677at 6491.848 kb on + strandat 6491.999 kb on + strandat 6491.999 kb on + strandat 6492.024 kb on + strandat 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.256 kb on + strand, within Pf6N2E2_5678at 6492.257 kb on - strand, within Pf6N2E2_5678at 6492.257 kb on - strand, within Pf6N2E2_5678at 6492.257 kb on - strand, within Pf6N2E2_5678at 6492.257 kb on - strand, within Pf6N2E2_5678at 6492.257 kb on - strand, within Pf6N2E2_5678at 6492.257 kb on - strand, within Pf6N2E2_5678at 6492.257 kb on - strand, within Pf6N2E2_5678at 6492.260 kb on + strand, within Pf6N2E2_5678at 6492.260 kb on + strand, within Pf6N2E2_5678at 6492.260 kb on + strand, within Pf6N2E2_5678at 6492.261 kb on - strand, within Pf6N2E2_5678at 6492.262 kb on + strand, within Pf6N2E2_5678at 6492.318 kb on + strand, within Pf6N2E2_5678at 6492.318 kb on + strand, within Pf6N2E2_5678at 6492.318 kb on + strand, within Pf6N2E2_5678at 6492.318 kb on + strand, within Pf6N2E2_5678at 6492.552 kb on + strand, within Pf6N2E2_5678at 6492.648 kb on + strandat 6492.649 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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6,490,767 + Pf6N2E2_5676 0.58 -0.3
6,490,767 + Pf6N2E2_5676 0.58 -0.2
6,490,767 + Pf6N2E2_5676 0.58 -1.7
6,490,767 + Pf6N2E2_5676 0.58 -0.2
6,490,767 + Pf6N2E2_5676 0.58 -0.5
6,490,770 + Pf6N2E2_5676 0.59 -0.3
6,491,374 + +0.7
6,491,374 + -1.2
6,491,377 + -0.3
6,491,377 + +1.0
6,491,377 + +0.2
6,491,377 + +0.0
6,491,377 + -0.3
6,491,407 + -1.0
6,491,407 + -0.5
6,491,407 + +1.0
6,491,444 + -0.5
6,491,444 + +0.0
6,491,469 + +1.5
6,491,469 + -0.3
6,491,469 + +0.5
6,491,508 + Pf6N2E2_5677 0.20 -0.6
6,491,848 + +0.6
6,491,999 + -1.4
6,491,999 + +0.1
6,492,024 + +0.6
6,492,256 + Pf6N2E2_5678 0.14 -0.8
6,492,256 + Pf6N2E2_5678 0.14 +0.1
6,492,256 + Pf6N2E2_5678 0.14 -0.6
6,492,256 + Pf6N2E2_5678 0.14 +0.7
6,492,256 + Pf6N2E2_5678 0.14 -0.6
6,492,256 + Pf6N2E2_5678 0.14 +0.6
6,492,256 + Pf6N2E2_5678 0.14 -0.4
6,492,256 + Pf6N2E2_5678 0.14 -0.8
6,492,256 + Pf6N2E2_5678 0.14 -0.3
6,492,257 - Pf6N2E2_5678 0.14 +0.4
6,492,257 - Pf6N2E2_5678 0.14 +0.5
6,492,257 - Pf6N2E2_5678 0.14 -0.6
6,492,257 - Pf6N2E2_5678 0.14 +0.3
6,492,257 - Pf6N2E2_5678 0.14 +0.3
6,492,257 - Pf6N2E2_5678 0.14 -1.8
6,492,257 - Pf6N2E2_5678 0.14 +1.0
6,492,260 + Pf6N2E2_5678 0.15 +0.7
6,492,260 + Pf6N2E2_5678 0.15 +2.3
6,492,260 + Pf6N2E2_5678 0.15 -0.7
6,492,261 - Pf6N2E2_5678 0.15 +1.0
6,492,262 + Pf6N2E2_5678 0.15 +0.2
6,492,318 + Pf6N2E2_5678 0.26 -0.2
6,492,318 + Pf6N2E2_5678 0.26 -0.3
6,492,318 + Pf6N2E2_5678 0.26 +0.0
6,492,318 + Pf6N2E2_5678 0.26 -0.4
6,492,552 + Pf6N2E2_5678 0.72 -0.1
6,492,648 + -1.0
6,492,649 - -1.8

Or see this region's nucleotide sequence