Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5666 and Pf6N2E2_5667 are separated by 54 nucleotides Pf6N2E2_5667 and Pf6N2E2_5668 overlap by 1 nucleotides
Pf6N2E2_5666 - Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109), at 6,478,652 to 6,479,671
_5666
Pf6N2E2_5667 - Arginine N-succinyltransferase (EC 2.3.1.109), at 6,479,726 to 6,480,751
_5667
Pf6N2E2_5668 - Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71), at 6,480,751 to 6,482,217
_5668
Position (kb)
6479
6480
6481 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6478.826 kb on + strand, within Pf6N2E2_5666 at 6478.827 kb on - strand, within Pf6N2E2_5666 at 6478.827 kb on - strand, within Pf6N2E2_5666 at 6478.913 kb on + strand, within Pf6N2E2_5666 at 6478.913 kb on + strand, within Pf6N2E2_5666 at 6478.914 kb on - strand, within Pf6N2E2_5666 at 6478.914 kb on - strand, within Pf6N2E2_5666 at 6479.030 kb on + strand, within Pf6N2E2_5666 at 6479.030 kb on + strand, within Pf6N2E2_5666 at 6479.030 kb on + strand, within Pf6N2E2_5666 at 6479.030 kb on + strand, within Pf6N2E2_5666 at 6479.031 kb on - strand, within Pf6N2E2_5666 at 6479.031 kb on - strand, within Pf6N2E2_5666 at 6479.031 kb on - strand, within Pf6N2E2_5666 at 6479.031 kb on - strand, within Pf6N2E2_5666 at 6479.217 kb on - strand, within Pf6N2E2_5666 at 6479.291 kb on + strand, within Pf6N2E2_5666 at 6479.291 kb on + strand, within Pf6N2E2_5666 at 6479.291 kb on + strand, within Pf6N2E2_5666 at 6479.291 kb on + strand, within Pf6N2E2_5666 at 6479.292 kb on - strand, within Pf6N2E2_5666 at 6479.295 kb on + strand, within Pf6N2E2_5666 at 6479.399 kb on + strand, within Pf6N2E2_5666 at 6479.399 kb on + strand, within Pf6N2E2_5666 at 6479.399 kb on + strand, within Pf6N2E2_5666 at 6479.399 kb on + strand, within Pf6N2E2_5666 at 6479.661 kb on + strand at 6479.667 kb on + strand at 6479.667 kb on + strand at 6479.667 kb on + strand at 6479.667 kb on + strand at 6479.667 kb on + strand at 6479.667 kb on + strand at 6479.667 kb on + strand at 6479.667 kb on + strand at 6479.667 kb on + strand at 6479.668 kb on - strand at 6479.668 kb on - strand at 6479.668 kb on - strand at 6479.668 kb on - strand at 6479.668 kb on - strand at 6479.668 kb on - strand at 6479.677 kb on + strand at 6479.677 kb on + strand at 6479.677 kb on + strand at 6479.678 kb on - strand at 6479.678 kb on - strand at 6479.722 kb on + strand at 6479.722 kb on + strand at 6479.722 kb on + strand at 6479.722 kb on + strand at 6479.723 kb on - strand at 6479.723 kb on - strand at 6479.723 kb on - strand at 6479.723 kb on - strand at 6479.723 kb on - strand at 6479.723 kb on - strand at 6479.723 kb on - strand at 6479.723 kb on - strand at 6479.894 kb on + strand, within Pf6N2E2_5667 at 6479.978 kb on + strand, within Pf6N2E2_5667 at 6479.978 kb on + strand, within Pf6N2E2_5667 at 6479.979 kb on - strand, within Pf6N2E2_5667 at 6479.979 kb on - strand, within Pf6N2E2_5667 at 6479.979 kb on - strand, within Pf6N2E2_5667 at 6480.029 kb on + strand, within Pf6N2E2_5667 at 6480.296 kb on + strand, within Pf6N2E2_5667 at 6480.297 kb on - strand, within Pf6N2E2_5667 at 6480.356 kb on + strand, within Pf6N2E2_5667 at 6480.357 kb on - strand, within Pf6N2E2_5667 at 6480.357 kb on - strand, within Pf6N2E2_5667 at 6480.357 kb on - strand, within Pf6N2E2_5667 at 6480.357 kb on - strand, within Pf6N2E2_5667 at 6480.357 kb on - strand, within Pf6N2E2_5667 at 6480.357 kb on - strand, within Pf6N2E2_5667 at 6480.455 kb on + strand, within Pf6N2E2_5667 at 6480.464 kb on + strand, within Pf6N2E2_5667 at 6480.465 kb on - strand, within Pf6N2E2_5667 at 6480.602 kb on - strand, within Pf6N2E2_5667 at 6480.763 kb on + strand at 6480.763 kb on + strand at 6481.087 kb on + strand, within Pf6N2E2_5668 at 6481.183 kb on + strand, within Pf6N2E2_5668 at 6481.332 kb on - strand, within Pf6N2E2_5668 at 6481.332 kb on - strand, within Pf6N2E2_5668 at 6481.540 kb on + strand, within Pf6N2E2_5668 at 6481.540 kb on + strand, within Pf6N2E2_5668 at 6481.549 kb on - strand, within Pf6N2E2_5668
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,478,826 + Pf6N2E2_5666 0.17 +1.8 6,478,827 - Pf6N2E2_5666 0.17 -1.0 6,478,827 - Pf6N2E2_5666 0.17 -0.5 6,478,913 + Pf6N2E2_5666 0.26 -0.1 6,478,913 + Pf6N2E2_5666 0.26 +1.6 6,478,914 - Pf6N2E2_5666 0.26 +0.3 6,478,914 - Pf6N2E2_5666 0.26 -1.4 6,479,030 + Pf6N2E2_5666 0.37 +0.5 6,479,030 + Pf6N2E2_5666 0.37 +0.0 6,479,030 + Pf6N2E2_5666 0.37 -0.9 6,479,030 + Pf6N2E2_5666 0.37 +0.4 6,479,031 - Pf6N2E2_5666 0.37 -1.7 6,479,031 - Pf6N2E2_5666 0.37 +1.0 6,479,031 - Pf6N2E2_5666 0.37 -1.1 6,479,031 - Pf6N2E2_5666 0.37 -0.3 6,479,217 - Pf6N2E2_5666 0.55 +0.0 6,479,291 + Pf6N2E2_5666 0.63 -0.2 6,479,291 + Pf6N2E2_5666 0.63 +0.5 6,479,291 + Pf6N2E2_5666 0.63 +0.4 6,479,291 + Pf6N2E2_5666 0.63 -1.0 6,479,292 - Pf6N2E2_5666 0.63 -0.1 6,479,295 + Pf6N2E2_5666 0.63 -1.2 6,479,399 + Pf6N2E2_5666 0.73 -0.1 6,479,399 + Pf6N2E2_5666 0.73 +0.5 6,479,399 + Pf6N2E2_5666 0.73 -1.5 6,479,399 + Pf6N2E2_5666 0.73 -1.4 6,479,661 + +0.6 6,479,667 + +1.1 6,479,667 + -0.3 6,479,667 + -0.7 6,479,667 + +1.0 6,479,667 + -1.7 6,479,667 + +0.0 6,479,667 + +0.5 6,479,667 + +0.5 6,479,667 + +0.1 6,479,668 - -0.8 6,479,668 - -1.0 6,479,668 - +1.0 6,479,668 - -1.2 6,479,668 - -1.4 6,479,668 - +0.0 6,479,677 + -0.3 6,479,677 + +0.5 6,479,677 + +1.9 6,479,678 - -2.7 6,479,678 - -0.6 6,479,722 + -1.8 6,479,722 + -2.0 6,479,722 + -0.8 6,479,722 + -0.2 6,479,723 - +1.0 6,479,723 - -0.4 6,479,723 - -2.8 6,479,723 - +0.8 6,479,723 - -0.5 6,479,723 - -1.1 6,479,723 - +0.5 6,479,723 - -0.4 6,479,894 + Pf6N2E2_5667 0.16 -0.1 6,479,978 + Pf6N2E2_5667 0.25 -0.8 6,479,978 + Pf6N2E2_5667 0.25 -1.7 6,479,979 - Pf6N2E2_5667 0.25 +0.2 6,479,979 - Pf6N2E2_5667 0.25 -2.5 6,479,979 - Pf6N2E2_5667 0.25 -1.0 6,480,029 + Pf6N2E2_5667 0.30 -1.0 6,480,296 + Pf6N2E2_5667 0.56 -0.7 6,480,297 - Pf6N2E2_5667 0.56 -1.0 6,480,356 + Pf6N2E2_5667 0.61 -1.0 6,480,357 - Pf6N2E2_5667 0.62 -1.4 6,480,357 - Pf6N2E2_5667 0.62 -1.3 6,480,357 - Pf6N2E2_5667 0.62 +1.8 6,480,357 - Pf6N2E2_5667 0.62 -0.5 6,480,357 - Pf6N2E2_5667 0.62 -0.1 6,480,357 - Pf6N2E2_5667 0.62 -0.0 6,480,455 + Pf6N2E2_5667 0.71 -0.4 6,480,464 + Pf6N2E2_5667 0.72 +0.4 6,480,465 - Pf6N2E2_5667 0.72 -1.3 6,480,602 - Pf6N2E2_5667 0.85 +0.0 6,480,763 + +1.2 6,480,763 + +0.9 6,481,087 + Pf6N2E2_5668 0.23 +1.0 6,481,183 + Pf6N2E2_5668 0.29 -0.4 6,481,332 - Pf6N2E2_5668 0.40 +0.5 6,481,332 - Pf6N2E2_5668 0.40 +2.1 6,481,540 + Pf6N2E2_5668 0.54 -0.4 6,481,540 + Pf6N2E2_5668 0.54 +0.9 6,481,549 - Pf6N2E2_5668 0.54 +0.9
Or see this region's nucleotide sequence