Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5660 and Pf6N2E2_5661 are separated by 90 nucleotides Pf6N2E2_5661 and Pf6N2E2_5662 overlap by 4 nucleotides Pf6N2E2_5662 and Pf6N2E2_5663 are separated by 56 nucleotides Pf6N2E2_5663 and Pf6N2E2_5664 are separated by 113 nucleotides
Pf6N2E2_5660 - L-Arginine ABC transporter, periplasmic substrate-binding component (from data) , at 6,472,608 to 6,473,381
_5660
Pf6N2E2_5661 - L-Arginine ABC transporter, permease protein AotQ (from data) , at 6,473,472 to 6,474,161
_5661
Pf6N2E2_5662 - L-Arginine ABC transporter, permease protein AotM (from data) , at 6,474,158 to 6,474,856
_5662
Pf6N2E2_5663 - L-Arginine ABC transporter, ATPase component (from data) , at 6,474,913 to 6,475,677
_5663
Pf6N2E2_5664 - Arginine pathway regulatory protein ArgR, repressor of arg regulon, at 6,475,791 to 6,476,771
_5664
Position (kb)
6474
6475 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6473.209 kb on + strand, within Pf6N2E2_5660 at 6473.209 kb on + strand, within Pf6N2E2_5660 at 6473.235 kb on + strand, within Pf6N2E2_5660 at 6473.235 kb on + strand, within Pf6N2E2_5660 at 6473.235 kb on + strand, within Pf6N2E2_5660 at 6473.236 kb on - strand, within Pf6N2E2_5660 at 6473.331 kb on + strand at 6473.331 kb on + strand at 6473.331 kb on + strand at 6473.332 kb on - strand at 6473.352 kb on + strand at 6473.352 kb on + strand at 6473.352 kb on + strand at 6473.353 kb on - strand at 6473.353 kb on - strand at 6473.353 kb on - strand at 6473.370 kb on + strand at 6473.370 kb on + strand at 6473.370 kb on + strand at 6473.371 kb on - strand at 6473.371 kb on - strand at 6473.371 kb on - strand at 6473.371 kb on - strand at 6473.371 kb on - strand at 6473.371 kb on - strand at 6473.379 kb on + strand at 6473.380 kb on - strand at 6473.625 kb on + strand, within Pf6N2E2_5661 at 6473.626 kb on - strand, within Pf6N2E2_5661 at 6473.646 kb on - strand, within Pf6N2E2_5661 at 6473.680 kb on - strand, within Pf6N2E2_5661 at 6473.733 kb on + strand, within Pf6N2E2_5661 at 6473.733 kb on + strand, within Pf6N2E2_5661 at 6473.733 kb on + strand, within Pf6N2E2_5661 at 6473.734 kb on - strand, within Pf6N2E2_5661 at 6473.734 kb on - strand, within Pf6N2E2_5661 at 6473.793 kb on + strand, within Pf6N2E2_5661 at 6473.794 kb on - strand, within Pf6N2E2_5661 at 6474.075 kb on + strand, within Pf6N2E2_5661 at 6474.075 kb on + strand, within Pf6N2E2_5661 at 6474.076 kb on - strand, within Pf6N2E2_5661 at 6474.136 kb on + strand at 6474.157 kb on + strand at 6474.157 kb on + strand at 6474.157 kb on + strand at 6474.157 kb on + strand at 6474.158 kb on - strand at 6474.158 kb on - strand at 6474.170 kb on + strand at 6474.182 kb on + strand at 6474.182 kb on + strand at 6474.182 kb on + strand at 6474.182 kb on + strand at 6474.182 kb on + strand at 6474.183 kb on - strand at 6474.183 kb on - strand at 6474.183 kb on - strand at 6474.183 kb on - strand at 6474.183 kb on - strand at 6474.200 kb on + strand at 6474.201 kb on - strand at 6474.329 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.335 kb on + strand, within Pf6N2E2_5662 at 6474.336 kb on - strand, within Pf6N2E2_5662 at 6474.336 kb on - strand, within Pf6N2E2_5662 at 6474.336 kb on - strand, within Pf6N2E2_5662 at 6474.336 kb on - strand, within Pf6N2E2_5662 at 6474.380 kb on + strand, within Pf6N2E2_5662 at 6474.381 kb on - strand, within Pf6N2E2_5662 at 6474.383 kb on + strand, within Pf6N2E2_5662 at 6474.383 kb on + strand, within Pf6N2E2_5662 at 6474.488 kb on + strand, within Pf6N2E2_5662 at 6474.489 kb on - strand, within Pf6N2E2_5662 at 6474.489 kb on - strand, within Pf6N2E2_5662 at 6474.629 kb on + strand, within Pf6N2E2_5662 at 6474.629 kb on + strand, within Pf6N2E2_5662 at 6474.630 kb on - strand, within Pf6N2E2_5662 at 6474.630 kb on - strand, within Pf6N2E2_5662 at 6474.635 kb on - strand, within Pf6N2E2_5662 at 6474.728 kb on + strand, within Pf6N2E2_5662 at 6474.729 kb on - strand, within Pf6N2E2_5662 at 6474.731 kb on + strand, within Pf6N2E2_5662 at 6474.731 kb on + strand, within Pf6N2E2_5662 at 6474.732 kb on - strand, within Pf6N2E2_5662 at 6474.732 kb on - strand, within Pf6N2E2_5662 at 6474.732 kb on - strand, within Pf6N2E2_5662 at 6474.732 kb on - strand, within Pf6N2E2_5662 at 6474.732 kb on - strand, within Pf6N2E2_5662 at 6474.749 kb on + strand, within Pf6N2E2_5662 at 6474.749 kb on + strand, within Pf6N2E2_5662 at 6474.750 kb on - strand, within Pf6N2E2_5662 at 6474.770 kb on + strand, within Pf6N2E2_5662 at 6474.771 kb on - strand, within Pf6N2E2_5662 at 6474.787 kb on + strand at 6474.834 kb on - strand at 6474.916 kb on + strand at 6474.916 kb on + strand at 6474.916 kb on + strand at 6474.916 kb on + strand at 6474.916 kb on + strand at 6474.916 kb on + strand at 6474.917 kb on - strand at 6474.917 kb on - strand at 6474.943 kb on - strand at 6474.943 kb on - strand at 6474.949 kb on + strand at 6475.041 kb on + strand, within Pf6N2E2_5663 at 6475.042 kb on - strand, within Pf6N2E2_5663 at 6475.042 kb on - strand, within Pf6N2E2_5663 at 6475.309 kb on + strand, within Pf6N2E2_5663 at 6475.309 kb on + strand, within Pf6N2E2_5663 at 6475.310 kb on - strand, within Pf6N2E2_5663 at 6475.467 kb on - strand, within Pf6N2E2_5663 at 6475.467 kb on - strand, within Pf6N2E2_5663 at 6475.587 kb on + strand, within Pf6N2E2_5663 at 6475.676 kb on - strand at 6475.676 kb on - strand at 6475.734 kb on + strand at 6475.735 kb on - strand at 6475.735 kb on - strand at 6475.762 kb on - strand at 6475.762 kb on - strand at 6475.808 kb on - strand at 6475.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,473,209 + Pf6N2E2_5660 0.78 -0.5 6,473,209 + Pf6N2E2_5660 0.78 -0.3 6,473,235 + Pf6N2E2_5660 0.81 -0.8 6,473,235 + Pf6N2E2_5660 0.81 +0.6 6,473,235 + Pf6N2E2_5660 0.81 -1.1 6,473,236 - Pf6N2E2_5660 0.81 -0.7 6,473,331 + +0.0 6,473,331 + +0.7 6,473,331 + -1.1 6,473,332 - -2.8 6,473,352 + +0.7 6,473,352 + +0.7 6,473,352 + -0.1 6,473,353 - -1.5 6,473,353 - -0.7 6,473,353 - -0.3 6,473,370 + +0.6 6,473,370 + -0.2 6,473,370 + +0.3 6,473,371 - -0.2 6,473,371 - +0.4 6,473,371 - -0.1 6,473,371 - +0.2 6,473,371 - -0.7 6,473,371 - -1.0 6,473,379 + +1.0 6,473,380 - -0.5 6,473,625 + Pf6N2E2_5661 0.22 +1.8 6,473,626 - Pf6N2E2_5661 0.22 -0.5 6,473,646 - Pf6N2E2_5661 0.25 -1.3 6,473,680 - Pf6N2E2_5661 0.30 -3.0 6,473,733 + Pf6N2E2_5661 0.38 -0.8 6,473,733 + Pf6N2E2_5661 0.38 -0.2 6,473,733 + Pf6N2E2_5661 0.38 +0.4 6,473,734 - Pf6N2E2_5661 0.38 -1.2 6,473,734 - Pf6N2E2_5661 0.38 -2.1 6,473,793 + Pf6N2E2_5661 0.47 -0.5 6,473,794 - Pf6N2E2_5661 0.47 -2.5 6,474,075 + Pf6N2E2_5661 0.87 -0.2 6,474,075 + Pf6N2E2_5661 0.87 -2.1 6,474,076 - Pf6N2E2_5661 0.88 -1.0 6,474,136 + +0.9 6,474,157 + -1.0 6,474,157 + +0.6 6,474,157 + -1.4 6,474,157 + -1.4 6,474,158 - -1.8 6,474,158 - -1.0 6,474,170 + -0.8 6,474,182 + -1.5 6,474,182 + -1.7 6,474,182 + -0.7 6,474,182 + +0.2 6,474,182 + -2.0 6,474,183 - -1.5 6,474,183 - +0.3 6,474,183 - -0.9 6,474,183 - -0.2 6,474,183 - -1.8 6,474,200 + -0.7 6,474,201 - +0.5 6,474,329 + Pf6N2E2_5662 0.24 -0.9 6,474,335 + Pf6N2E2_5662 0.25 -0.7 6,474,335 + Pf6N2E2_5662 0.25 -0.3 6,474,335 + Pf6N2E2_5662 0.25 -0.2 6,474,335 + Pf6N2E2_5662 0.25 -0.4 6,474,335 + Pf6N2E2_5662 0.25 -0.1 6,474,335 + Pf6N2E2_5662 0.25 -0.5 6,474,335 + Pf6N2E2_5662 0.25 -2.0 6,474,335 + Pf6N2E2_5662 0.25 -0.7 6,474,335 + Pf6N2E2_5662 0.25 -2.0 6,474,336 - Pf6N2E2_5662 0.25 -0.6 6,474,336 - Pf6N2E2_5662 0.25 -2.5 6,474,336 - Pf6N2E2_5662 0.25 -1.5 6,474,336 - Pf6N2E2_5662 0.25 -2.5 6,474,380 + Pf6N2E2_5662 0.32 -0.1 6,474,381 - Pf6N2E2_5662 0.32 -2.3 6,474,383 + Pf6N2E2_5662 0.32 -1.8 6,474,383 + Pf6N2E2_5662 0.32 -1.1 6,474,488 + Pf6N2E2_5662 0.47 -1.0 6,474,489 - Pf6N2E2_5662 0.47 +1.0 6,474,489 - Pf6N2E2_5662 0.47 -0.2 6,474,629 + Pf6N2E2_5662 0.67 +0.6 6,474,629 + Pf6N2E2_5662 0.67 +0.9 6,474,630 - Pf6N2E2_5662 0.68 -1.5 6,474,630 - Pf6N2E2_5662 0.68 -1.9 6,474,635 - Pf6N2E2_5662 0.68 +0.0 6,474,728 + Pf6N2E2_5662 0.82 -0.3 6,474,729 - Pf6N2E2_5662 0.82 -0.4 6,474,731 + Pf6N2E2_5662 0.82 -0.6 6,474,731 + Pf6N2E2_5662 0.82 -0.2 6,474,732 - Pf6N2E2_5662 0.82 -3.0 6,474,732 - Pf6N2E2_5662 0.82 -1.3 6,474,732 - Pf6N2E2_5662 0.82 -0.3 6,474,732 - Pf6N2E2_5662 0.82 -1.8 6,474,732 - Pf6N2E2_5662 0.82 -0.7 6,474,749 + Pf6N2E2_5662 0.85 -0.8 6,474,749 + Pf6N2E2_5662 0.85 +1.6 6,474,750 - Pf6N2E2_5662 0.85 -1.7 6,474,770 + Pf6N2E2_5662 0.88 +0.0 6,474,771 - Pf6N2E2_5662 0.88 -1.5 6,474,787 + +0.3 6,474,834 - +0.2 6,474,916 + -1.1 6,474,916 + -3.0 6,474,916 + +0.3 6,474,916 + -0.3 6,474,916 + -1.0 6,474,916 + -1.5 6,474,917 - -0.8 6,474,917 - -0.7 6,474,943 - +1.0 6,474,943 - -2.3 6,474,949 + -2.0 6,475,041 + Pf6N2E2_5663 0.17 +0.9 6,475,042 - Pf6N2E2_5663 0.17 -2.5 6,475,042 - Pf6N2E2_5663 0.17 -0.2 6,475,309 + Pf6N2E2_5663 0.52 +0.1 6,475,309 + Pf6N2E2_5663 0.52 -1.1 6,475,310 - Pf6N2E2_5663 0.52 -0.8 6,475,467 - Pf6N2E2_5663 0.72 -2.4 6,475,467 - Pf6N2E2_5663 0.72 +1.0 6,475,587 + Pf6N2E2_5663 0.88 +0.0 6,475,676 - -1.0 6,475,676 - -0.4 6,475,734 + +0.3 6,475,735 - +0.4 6,475,735 - -2.5 6,475,762 - +1.6 6,475,762 - -0.5 6,475,808 - +1.6 6,475,833 - -0.8
Or see this region's nucleotide sequence