Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5249 and Pf6N2E2_5250 are separated by 142 nucleotides Pf6N2E2_5250 and Pf6N2E2_5251 are separated by 10 nucleotides Pf6N2E2_5251 and Pf6N2E2_5252 are separated by 39 nucleotides Pf6N2E2_5252 and Pf6N2E2_5253 are separated by 77 nucleotides Pf6N2E2_5253 and Pf6N2E2_5254 are separated by 10 nucleotides
Pf6N2E2_5249 - Glycerol-3-phosphate O-acyltransferase, at 6,043,273 to 6,044,436
_5249
Pf6N2E2_5250 - Restriction endonuclease, at 6,044,579 to 6,044,761
_5250
Pf6N2E2_5251 - FIG00954698: hypothetical protein, at 6,044,772 to 6,045,032
_5251
Pf6N2E2_5252 - FIG00956396: hypothetical protein, at 6,045,072 to 6,045,695
_5252
Pf6N2E2_5253 - Additional periplasmic component NikK of nickel ECF transporter, at 6,045,773 to 6,046,492
_5253
Pf6N2E2_5254 - Ferrichrome-iron receptor, at 6,046,503 to 6,048,608
_5254
Position (kb)
6045
6046 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6044.466 kb on - strand at 6044.466 kb on - strand at 6044.466 kb on - strand at 6044.466 kb on - strand at 6044.470 kb on - strand at 6044.470 kb on - strand at 6044.470 kb on - strand at 6044.470 kb on - strand at 6044.512 kb on - strand at 6044.512 kb on - strand at 6044.527 kb on - strand at 6044.553 kb on - strand at 6044.553 kb on - strand at 6044.553 kb on - strand at 6044.598 kb on - strand, within Pf6N2E2_5250 at 6044.609 kb on + strand, within Pf6N2E2_5250 at 6044.610 kb on - strand, within Pf6N2E2_5250 at 6044.663 kb on + strand, within Pf6N2E2_5250 at 6044.663 kb on + strand, within Pf6N2E2_5250 at 6044.663 kb on + strand, within Pf6N2E2_5250 at 6044.663 kb on + strand, within Pf6N2E2_5250 at 6044.664 kb on - strand, within Pf6N2E2_5250 at 6044.664 kb on - strand, within Pf6N2E2_5250 at 6044.664 kb on - strand, within Pf6N2E2_5250 at 6044.664 kb on - strand at 6044.784 kb on + strand at 6044.785 kb on - strand at 6044.943 kb on + strand, within Pf6N2E2_5251 at 6044.967 kb on + strand, within Pf6N2E2_5251 at 6044.968 kb on - strand, within Pf6N2E2_5251 at 6044.968 kb on - strand, within Pf6N2E2_5251 at 6045.009 kb on + strand at 6045.009 kb on + strand at 6045.009 kb on + strand at 6045.009 kb on + strand at 6045.009 kb on + strand at 6045.010 kb on - strand at 6045.030 kb on + strand at 6045.030 kb on + strand at 6045.030 kb on + strand at 6045.031 kb on - strand at 6045.366 kb on + strand, within Pf6N2E2_5252 at 6045.366 kb on + strand, within Pf6N2E2_5252 at 6045.367 kb on - strand, within Pf6N2E2_5252 at 6045.644 kb on + strand at 6045.644 kb on + strand at 6045.760 kb on + strand at 6045.760 kb on + strand at 6045.760 kb on + strand at 6045.760 kb on + strand at 6045.760 kb on + strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.761 kb on - strand at 6045.938 kb on + strand, within Pf6N2E2_5253 at 6046.113 kb on + strand, within Pf6N2E2_5253 at 6046.113 kb on + strand, within Pf6N2E2_5253 at 6046.113 kb on + strand, within Pf6N2E2_5253 at 6046.114 kb on - strand, within Pf6N2E2_5253 at 6046.114 kb on - strand, within Pf6N2E2_5253 at 6046.114 kb on - strand, within Pf6N2E2_5253 at 6046.114 kb on - strand, within Pf6N2E2_5253 at 6046.128 kb on + strand, within Pf6N2E2_5253 at 6046.128 kb on + strand, within Pf6N2E2_5253 at 6046.129 kb on - strand, within Pf6N2E2_5253 at 6046.129 kb on - strand, within Pf6N2E2_5253 at 6046.138 kb on + strand, within Pf6N2E2_5253 at 6046.138 kb on + strand, within Pf6N2E2_5253 at 6046.138 kb on + strand, within Pf6N2E2_5253 at 6046.139 kb on - strand, within Pf6N2E2_5253 at 6046.139 kb on - strand, within Pf6N2E2_5253 at 6046.139 kb on - strand, within Pf6N2E2_5253 at 6046.139 kb on - strand, within Pf6N2E2_5253 at 6046.292 kb on + strand, within Pf6N2E2_5253 at 6046.326 kb on + strand, within Pf6N2E2_5253 at 6046.326 kb on + strand, within Pf6N2E2_5253 at 6046.326 kb on + strand, within Pf6N2E2_5253 at 6046.327 kb on - strand, within Pf6N2E2_5253 at 6046.386 kb on + strand, within Pf6N2E2_5253 at 6046.386 kb on + strand, within Pf6N2E2_5253 at 6046.505 kb on - strand at 6046.505 kb on - strand at 6046.505 kb on - strand at 6046.505 kb on - strand at 6046.506 kb on + strand at 6046.506 kb on + strand at 6046.506 kb on + strand at 6046.506 kb on + strand at 6046.506 kb on + strand at 6046.506 kb on + strand at 6046.507 kb on - strand at 6046.507 kb on - strand at 6046.507 kb on - strand at 6046.561 kb on + strand at 6046.561 kb on + strand at 6046.571 kb on - strand at 6046.571 kb on - strand at 6046.571 kb on - strand at 6046.583 kb on - strand at 6046.583 kb on - strand at 6046.583 kb on - strand at 6046.585 kb on + strand at 6046.645 kb on + strand at 6046.645 kb on + strand at 6046.645 kb on + strand at 6046.645 kb on + strand at 6046.645 kb on + strand at 6046.645 kb on + strand at 6046.646 kb on - strand at 6046.646 kb on - strand at 6046.646 kb on - strand at 6046.646 kb on - strand at 6046.672 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,044,466 - -0.9 6,044,466 - -0.1 6,044,466 - +0.6 6,044,466 - -0.6 6,044,470 - -1.2 6,044,470 - +0.9 6,044,470 - +1.6 6,044,470 - +0.7 6,044,512 - -0.8 6,044,512 - +0.9 6,044,527 - -0.8 6,044,553 - -0.9 6,044,553 - +1.8 6,044,553 - -0.1 6,044,598 - Pf6N2E2_5250 0.10 +0.9 6,044,609 + Pf6N2E2_5250 0.16 +0.7 6,044,610 - Pf6N2E2_5250 0.17 +0.9 6,044,663 + Pf6N2E2_5250 0.46 +0.0 6,044,663 + Pf6N2E2_5250 0.46 +0.1 6,044,663 + Pf6N2E2_5250 0.46 +1.0 6,044,663 + Pf6N2E2_5250 0.46 +0.2 6,044,664 - Pf6N2E2_5250 0.46 +0.6 6,044,664 - Pf6N2E2_5250 0.46 -0.6 6,044,664 - Pf6N2E2_5250 0.46 +2.0 6,044,664 - -0.4 6,044,784 + -0.0 6,044,785 - -0.9 6,044,943 + Pf6N2E2_5251 0.66 +0.7 6,044,967 + Pf6N2E2_5251 0.75 +0.2 6,044,968 - Pf6N2E2_5251 0.75 -0.8 6,044,968 - Pf6N2E2_5251 0.75 -0.2 6,045,009 + +0.2 6,045,009 + -0.2 6,045,009 + -0.8 6,045,009 + +0.9 6,045,009 + -0.2 6,045,010 - -1.3 6,045,030 + +0.2 6,045,030 + -0.0 6,045,030 + +0.1 6,045,031 - -0.0 6,045,366 + Pf6N2E2_5252 0.47 +0.2 6,045,366 + Pf6N2E2_5252 0.47 -3.1 6,045,367 - Pf6N2E2_5252 0.47 -2.0 6,045,644 + -0.1 6,045,644 + +0.5 6,045,760 + -0.2 6,045,760 + +0.0 6,045,760 + +0.7 6,045,760 + +0.1 6,045,760 + +0.5 6,045,761 - -0.4 6,045,761 - +1.7 6,045,761 - -0.8 6,045,761 - +0.2 6,045,761 - +0.9 6,045,761 - +0.4 6,045,761 - +0.3 6,045,761 - +0.1 6,045,761 - -0.7 6,045,761 - -0.4 6,045,938 + Pf6N2E2_5253 0.23 +0.8 6,046,113 + Pf6N2E2_5253 0.47 -0.2 6,046,113 + Pf6N2E2_5253 0.47 +0.2 6,046,113 + Pf6N2E2_5253 0.47 -0.7 6,046,114 - Pf6N2E2_5253 0.47 +0.6 6,046,114 - Pf6N2E2_5253 0.47 -0.3 6,046,114 - Pf6N2E2_5253 0.47 -1.4 6,046,114 - Pf6N2E2_5253 0.47 -1.3 6,046,128 + Pf6N2E2_5253 0.49 -0.4 6,046,128 + Pf6N2E2_5253 0.49 +0.9 6,046,129 - Pf6N2E2_5253 0.49 +1.1 6,046,129 - Pf6N2E2_5253 0.49 +0.7 6,046,138 + Pf6N2E2_5253 0.51 +0.5 6,046,138 + Pf6N2E2_5253 0.51 +0.4 6,046,138 + Pf6N2E2_5253 0.51 +0.1 6,046,139 - Pf6N2E2_5253 0.51 +0.4 6,046,139 - Pf6N2E2_5253 0.51 +1.6 6,046,139 - Pf6N2E2_5253 0.51 -1.2 6,046,139 - Pf6N2E2_5253 0.51 -0.2 6,046,292 + Pf6N2E2_5253 0.72 +0.2 6,046,326 + Pf6N2E2_5253 0.77 +0.8 6,046,326 + Pf6N2E2_5253 0.77 +0.4 6,046,326 + Pf6N2E2_5253 0.77 +0.6 6,046,327 - Pf6N2E2_5253 0.77 -0.4 6,046,386 + Pf6N2E2_5253 0.85 +0.3 6,046,386 + Pf6N2E2_5253 0.85 +0.8 6,046,505 - +0.6 6,046,505 - -0.0 6,046,505 - -1.5 6,046,505 - +1.9 6,046,506 + +1.3 6,046,506 + -0.4 6,046,506 + -0.0 6,046,506 + +0.7 6,046,506 + +1.2 6,046,506 + +1.5 6,046,507 - -2.9 6,046,507 - -0.5 6,046,507 - -1.1 6,046,561 + -0.4 6,046,561 + -0.1 6,046,571 - -0.2 6,046,571 - +0.4 6,046,571 - +0.2 6,046,583 - -0.2 6,046,583 - +0.4 6,046,583 - +0.5 6,046,585 + -1.0 6,046,645 + -0.3 6,046,645 + +1.2 6,046,645 + +0.1 6,046,645 + -0.1 6,046,645 + -0.5 6,046,645 + -1.7 6,046,646 - -0.2 6,046,646 - -0.4 6,046,646 - -0.0 6,046,646 - -1.4 6,046,672 + -0.8
Or see this region's nucleotide sequence