Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5234 and Pf6N2E2_5235 are separated by 2 nucleotides Pf6N2E2_5235 and Pf6N2E2_5236 are separated by 62 nucleotides Pf6N2E2_5236 and Pf6N2E2_5237 overlap by 21 nucleotides Pf6N2E2_5237 and Pf6N2E2_5238 are separated by 3 nucleotides
Pf6N2E2_5234 - Uracil permease, at 6,032,089 to 6,033,363
_5234
Pf6N2E2_5235 - Uracil phosphoribosyltransferase (EC 2.4.2.9), at 6,033,366 to 6,034,004
_5235
Pf6N2E2_5236 - hypothetical protein, at 6,034,067 to 6,034,189
_5236
Pf6N2E2_5237 - Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8), at 6,034,169 to 6,034,726
_5237
Pf6N2E2_5238 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 6,034,730 to 6,035,203
_5238
Position (kb)
6034
6035 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6033.074 kb on + strand, within Pf6N2E2_5234 at 6033.075 kb on - strand, within Pf6N2E2_5234 at 6033.075 kb on - strand, within Pf6N2E2_5234 at 6033.249 kb on + strand at 6033.250 kb on - strand at 6033.280 kb on + strand at 6033.280 kb on + strand at 6033.430 kb on + strand, within Pf6N2E2_5235 at 6033.430 kb on + strand, within Pf6N2E2_5235 at 6033.430 kb on + strand, within Pf6N2E2_5235 at 6033.469 kb on + strand, within Pf6N2E2_5235 at 6033.469 kb on + strand, within Pf6N2E2_5235 at 6033.469 kb on + strand, within Pf6N2E2_5235 at 6033.469 kb on + strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.470 kb on - strand, within Pf6N2E2_5235 at 6033.667 kb on + strand, within Pf6N2E2_5235 at 6033.667 kb on + strand, within Pf6N2E2_5235 at 6033.668 kb on - strand, within Pf6N2E2_5235 at 6033.668 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.851 kb on - strand, within Pf6N2E2_5235 at 6033.883 kb on + strand, within Pf6N2E2_5235 at 6033.883 kb on + strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6033.884 kb on - strand, within Pf6N2E2_5235 at 6034.041 kb on - strand at 6034.045 kb on + strand at 6034.046 kb on - strand at 6034.050 kb on + strand at 6034.050 kb on + strand at 6034.050 kb on + strand at 6034.051 kb on - strand at 6034.051 kb on - strand at 6034.071 kb on - strand at 6034.077 kb on + strand at 6034.077 kb on + strand at 6034.078 kb on - strand at 6034.088 kb on - strand, within Pf6N2E2_5236 at 6034.088 kb on - strand, within Pf6N2E2_5236 at 6034.125 kb on - strand, within Pf6N2E2_5236 at 6034.125 kb on - strand, within Pf6N2E2_5236 at 6034.224 kb on - strand at 6034.383 kb on - strand, within Pf6N2E2_5237 at 6034.650 kb on - strand, within Pf6N2E2_5237 at 6034.650 kb on - strand, within Pf6N2E2_5237 at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.673 kb on + strand at 6034.674 kb on - strand at 6034.674 kb on - strand at 6034.674 kb on - strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.724 kb on + strand at 6034.725 kb on - strand at 6034.725 kb on - strand at 6034.816 kb on + strand, within Pf6N2E2_5238 at 6034.816 kb on + strand, within Pf6N2E2_5238 at 6034.817 kb on - strand, within Pf6N2E2_5238 at 6034.817 kb on - strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.877 kb on + strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6034.878 kb on - strand, within Pf6N2E2_5238 at 6035.042 kb on + strand, within Pf6N2E2_5238
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 6,033,074 + Pf6N2E2_5234 0.77 -0.2 6,033,075 - Pf6N2E2_5234 0.77 -0.5 6,033,075 - Pf6N2E2_5234 0.77 +0.0 6,033,249 + +0.1 6,033,250 - +0.6 6,033,280 + -1.1 6,033,280 + -0.6 6,033,430 + Pf6N2E2_5235 0.10 +0.6 6,033,430 + Pf6N2E2_5235 0.10 -0.4 6,033,430 + Pf6N2E2_5235 0.10 -0.2 6,033,469 + Pf6N2E2_5235 0.16 +0.2 6,033,469 + Pf6N2E2_5235 0.16 +0.3 6,033,469 + Pf6N2E2_5235 0.16 -0.0 6,033,469 + Pf6N2E2_5235 0.16 -0.2 6,033,470 - Pf6N2E2_5235 0.16 -1.4 6,033,470 - Pf6N2E2_5235 0.16 -1.1 6,033,470 - Pf6N2E2_5235 0.16 -0.0 6,033,470 - Pf6N2E2_5235 0.16 -0.2 6,033,470 - Pf6N2E2_5235 0.16 -0.1 6,033,470 - Pf6N2E2_5235 0.16 -0.2 6,033,470 - Pf6N2E2_5235 0.16 -1.1 6,033,470 - Pf6N2E2_5235 0.16 -0.1 6,033,667 + Pf6N2E2_5235 0.47 -1.0 6,033,667 + Pf6N2E2_5235 0.47 +0.8 6,033,668 - Pf6N2E2_5235 0.47 -0.8 6,033,668 - Pf6N2E2_5235 0.47 -0.7 6,033,851 - Pf6N2E2_5235 0.76 +0.8 6,033,851 - Pf6N2E2_5235 0.76 -0.4 6,033,851 - Pf6N2E2_5235 0.76 +0.2 6,033,851 - Pf6N2E2_5235 0.76 +1.0 6,033,851 - Pf6N2E2_5235 0.76 +0.6 6,033,883 + Pf6N2E2_5235 0.81 -0.2 6,033,883 + Pf6N2E2_5235 0.81 +0.2 6,033,884 - Pf6N2E2_5235 0.81 -2.2 6,033,884 - Pf6N2E2_5235 0.81 +0.8 6,033,884 - Pf6N2E2_5235 0.81 -0.3 6,033,884 - Pf6N2E2_5235 0.81 -0.4 6,033,884 - Pf6N2E2_5235 0.81 +0.1 6,034,041 - +0.8 6,034,045 + +2.1 6,034,046 - -0.1 6,034,050 + +1.8 6,034,050 + +0.7 6,034,050 + +0.9 6,034,051 - -0.3 6,034,051 - +0.2 6,034,071 - -1.4 6,034,077 + +0.4 6,034,077 + +0.4 6,034,078 - +0.2 6,034,088 - Pf6N2E2_5236 0.17 +0.1 6,034,088 - Pf6N2E2_5236 0.17 -0.5 6,034,125 - Pf6N2E2_5236 0.47 -1.3 6,034,125 - Pf6N2E2_5236 0.47 -0.5 6,034,224 - -0.0 6,034,383 - Pf6N2E2_5237 0.38 -0.7 6,034,650 - Pf6N2E2_5237 0.86 +0.8 6,034,650 - Pf6N2E2_5237 0.86 -0.2 6,034,673 + +0.5 6,034,673 + -0.8 6,034,673 + -0.7 6,034,673 + +0.2 6,034,674 - -1.0 6,034,674 - -0.2 6,034,674 - -0.0 6,034,724 + +0.7 6,034,724 + +0.1 6,034,724 + -0.8 6,034,724 + +0.4 6,034,725 - -0.1 6,034,725 - +1.2 6,034,816 + Pf6N2E2_5238 0.18 -1.0 6,034,816 + Pf6N2E2_5238 0.18 +0.2 6,034,817 - Pf6N2E2_5238 0.18 +0.7 6,034,817 - Pf6N2E2_5238 0.18 +1.5 6,034,877 + Pf6N2E2_5238 0.31 +1.0 6,034,877 + Pf6N2E2_5238 0.31 +0.1 6,034,877 + Pf6N2E2_5238 0.31 -0.3 6,034,877 + Pf6N2E2_5238 0.31 +0.6 6,034,878 - Pf6N2E2_5238 0.31 +1.1 6,034,878 - Pf6N2E2_5238 0.31 -0.8 6,034,878 - Pf6N2E2_5238 0.31 +0.5 6,034,878 - Pf6N2E2_5238 0.31 -0.5 6,035,042 + Pf6N2E2_5238 0.66 +0.1
Or see this region's nucleotide sequence