Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6153 and Pf6N2E2_6154 are separated by 167 nucleotides Pf6N2E2_6154 and Pf6N2E2_5181 are separated by 146 nucleotides Pf6N2E2_5181 and Pf6N2E2_5182 overlap by 14 nucleotides Pf6N2E2_5182 and Pf6N2E2_5183 overlap by 17 nucleotides
Pf6N2E2_6153 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 5,971,888 to 5,974,777
_6153
Pf6N2E2_6154 - 5S RNA, at 5,974,945 to 5,975,064
_6154
Pf6N2E2_5181 - Paraquat-inducible protein A, at 5,975,211 to 5,975,870
_5181
Pf6N2E2_5182 - Paraquat-inducible protein A, at 5,975,857 to 5,976,480
_5182
Pf6N2E2_5183 - Paraquat-inducible protein B, at 5,976,464 to 5,978,776
_5183
Position (kb)
5975
5976 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5975.096 kb on + strand at 5975.120 kb on - strand at 5975.120 kb on - strand at 5975.156 kb on + strand at 5975.156 kb on + strand at 5975.156 kb on + strand at 5975.156 kb on + strand at 5975.156 kb on + strand at 5975.156 kb on + strand at 5975.157 kb on - strand at 5975.175 kb on + strand at 5975.175 kb on + strand at 5975.180 kb on + strand at 5975.181 kb on - strand at 5975.191 kb on + strand at 5975.191 kb on + strand at 5975.191 kb on + strand at 5975.191 kb on + strand at 5975.191 kb on + strand at 5975.211 kb on - strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.234 kb on + strand at 5975.235 kb on - strand at 5975.235 kb on - strand at 5975.235 kb on - strand at 5975.235 kb on - strand at 5975.355 kb on + strand, within Pf6N2E2_5181 at 5975.355 kb on + strand, within Pf6N2E2_5181 at 5975.355 kb on + strand, within Pf6N2E2_5181 at 5975.356 kb on - strand, within Pf6N2E2_5181 at 5975.356 kb on - strand, within Pf6N2E2_5181 at 5975.356 kb on - strand, within Pf6N2E2_5181 at 5975.374 kb on + strand, within Pf6N2E2_5181 at 5975.374 kb on + strand, within Pf6N2E2_5181 at 5975.374 kb on + strand, within Pf6N2E2_5181 at 5975.374 kb on + strand, within Pf6N2E2_5181 at 5975.374 kb on + strand, within Pf6N2E2_5181 at 5975.374 kb on + strand, within Pf6N2E2_5181 at 5975.375 kb on - strand, within Pf6N2E2_5181 at 5975.375 kb on - strand, within Pf6N2E2_5181 at 5975.375 kb on - strand, within Pf6N2E2_5181 at 5975.375 kb on - strand, within Pf6N2E2_5181 at 5975.385 kb on - strand, within Pf6N2E2_5181 at 5975.416 kb on + strand, within Pf6N2E2_5181 at 5975.416 kb on + strand, within Pf6N2E2_5181 at 5975.416 kb on + strand, within Pf6N2E2_5181 at 5975.416 kb on + strand, within Pf6N2E2_5181 at 5975.417 kb on - strand, within Pf6N2E2_5181 at 5975.417 kb on - strand, within Pf6N2E2_5181 at 5975.417 kb on - strand, within Pf6N2E2_5181 at 5975.417 kb on - strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.418 kb on + strand, within Pf6N2E2_5181 at 5975.419 kb on - strand, within Pf6N2E2_5181 at 5975.419 kb on - strand, within Pf6N2E2_5181 at 5975.419 kb on - strand, within Pf6N2E2_5181 at 5975.419 kb on - strand, within Pf6N2E2_5181 at 5975.419 kb on - strand, within Pf6N2E2_5181 at 5975.419 kb on - strand, within Pf6N2E2_5181 at 5975.419 kb on - strand, within Pf6N2E2_5181 at 5975.419 kb on - strand, within Pf6N2E2_5181 at 5975.437 kb on + strand, within Pf6N2E2_5181 at 5975.627 kb on + strand, within Pf6N2E2_5181 at 5975.628 kb on - strand, within Pf6N2E2_5181 at 5975.631 kb on + strand, within Pf6N2E2_5181 at 5975.631 kb on + strand, within Pf6N2E2_5181 at 5975.632 kb on - strand, within Pf6N2E2_5181 at 5975.632 kb on - strand, within Pf6N2E2_5181 at 5975.647 kb on - strand, within Pf6N2E2_5181 at 5975.656 kb on - strand, within Pf6N2E2_5181 at 5975.665 kb on + strand, within Pf6N2E2_5181 at 5975.665 kb on + strand, within Pf6N2E2_5181 at 5975.666 kb on - strand, within Pf6N2E2_5181 at 5975.692 kb on - strand, within Pf6N2E2_5181 at 5975.749 kb on + strand, within Pf6N2E2_5181 at 5975.749 kb on + strand, within Pf6N2E2_5181 at 5975.750 kb on - strand, within Pf6N2E2_5181 at 5976.028 kb on + strand, within Pf6N2E2_5182 at 5976.029 kb on - strand, within Pf6N2E2_5182 at 5976.029 kb on - strand, within Pf6N2E2_5182 at 5976.029 kb on - strand, within Pf6N2E2_5182 at 5976.029 kb on - strand, within Pf6N2E2_5182 at 5976.031 kb on - strand, within Pf6N2E2_5182 at 5976.060 kb on + strand, within Pf6N2E2_5182 at 5976.061 kb on - strand, within Pf6N2E2_5182 at 5976.234 kb on + strand, within Pf6N2E2_5182 at 5976.234 kb on + strand, within Pf6N2E2_5182 at 5976.234 kb on + strand, within Pf6N2E2_5182 at 5976.234 kb on + strand, within Pf6N2E2_5182 at 5976.234 kb on + strand, within Pf6N2E2_5182 at 5976.235 kb on - strand, within Pf6N2E2_5182 at 5976.258 kb on + strand, within Pf6N2E2_5182 at 5976.258 kb on + strand, within Pf6N2E2_5182 at 5976.258 kb on + strand, within Pf6N2E2_5182 at 5976.258 kb on + strand, within Pf6N2E2_5182 at 5976.258 kb on + strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.259 kb on - strand, within Pf6N2E2_5182 at 5976.262 kb on + strand, within Pf6N2E2_5182 at 5976.262 kb on + strand, within Pf6N2E2_5182 at 5976.263 kb on - strand, within Pf6N2E2_5182 at 5976.582 kb on - strand at 5976.582 kb on - strand at 5976.659 kb on + strand at 5976.765 kb on - strand, within Pf6N2E2_5183 at 5976.765 kb on - strand, within Pf6N2E2_5183 at 5976.765 kb on - strand, within Pf6N2E2_5183 at 5976.851 kb on - strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.854 kb on + strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183 at 5976.855 kb on - strand, within Pf6N2E2_5183
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 5,975,096 + +0.5 5,975,120 - -0.9 5,975,120 - -0.9 5,975,156 + +1.1 5,975,156 + +1.0 5,975,156 + -0.5 5,975,156 + +0.3 5,975,156 + -0.1 5,975,156 + +0.3 5,975,157 - -0.4 5,975,175 + -0.3 5,975,175 + +1.2 5,975,180 + +2.3 5,975,181 - -1.7 5,975,191 + +0.1 5,975,191 + -0.1 5,975,191 + +0.7 5,975,191 + +0.3 5,975,191 + +1.3 5,975,211 - -1.1 5,975,234 + -0.5 5,975,234 + -0.6 5,975,234 + +0.7 5,975,234 + +0.1 5,975,234 + -0.5 5,975,234 + -0.0 5,975,234 + +0.0 5,975,234 + -0.9 5,975,234 + -1.2 5,975,234 + +0.8 5,975,235 - +0.8 5,975,235 - +0.6 5,975,235 - -0.9 5,975,235 - -0.5 5,975,355 + Pf6N2E2_5181 0.22 -0.7 5,975,355 + Pf6N2E2_5181 0.22 -0.6 5,975,355 + Pf6N2E2_5181 0.22 -0.8 5,975,356 - Pf6N2E2_5181 0.22 -1.9 5,975,356 - Pf6N2E2_5181 0.22 -1.0 5,975,356 - Pf6N2E2_5181 0.22 -1.3 5,975,374 + Pf6N2E2_5181 0.25 -0.6 5,975,374 + Pf6N2E2_5181 0.25 +0.0 5,975,374 + Pf6N2E2_5181 0.25 -1.1 5,975,374 + Pf6N2E2_5181 0.25 -1.5 5,975,374 + Pf6N2E2_5181 0.25 +1.6 5,975,374 + Pf6N2E2_5181 0.25 -0.7 5,975,375 - Pf6N2E2_5181 0.25 +0.7 5,975,375 - Pf6N2E2_5181 0.25 +0.6 5,975,375 - Pf6N2E2_5181 0.25 -1.9 5,975,375 - Pf6N2E2_5181 0.25 -1.3 5,975,385 - Pf6N2E2_5181 0.26 -0.0 5,975,416 + Pf6N2E2_5181 0.31 -1.5 5,975,416 + Pf6N2E2_5181 0.31 -0.1 5,975,416 + Pf6N2E2_5181 0.31 -1.3 5,975,416 + Pf6N2E2_5181 0.31 -1.3 5,975,417 - Pf6N2E2_5181 0.31 -1.2 5,975,417 - Pf6N2E2_5181 0.31 -1.9 5,975,417 - Pf6N2E2_5181 0.31 -1.3 5,975,417 - Pf6N2E2_5181 0.31 -1.3 5,975,418 + Pf6N2E2_5181 0.31 -0.7 5,975,418 + Pf6N2E2_5181 0.31 -1.0 5,975,418 + Pf6N2E2_5181 0.31 -1.6 5,975,418 + Pf6N2E2_5181 0.31 -0.7 5,975,418 + Pf6N2E2_5181 0.31 -1.6 5,975,418 + Pf6N2E2_5181 0.31 +0.9 5,975,418 + Pf6N2E2_5181 0.31 -1.7 5,975,418 + Pf6N2E2_5181 0.31 -0.6 5,975,418 + Pf6N2E2_5181 0.31 -1.0 5,975,418 + Pf6N2E2_5181 0.31 -1.9 5,975,418 + Pf6N2E2_5181 0.31 -2.7 5,975,419 - Pf6N2E2_5181 0.32 -1.6 5,975,419 - Pf6N2E2_5181 0.32 -0.7 5,975,419 - Pf6N2E2_5181 0.32 -0.0 5,975,419 - Pf6N2E2_5181 0.32 +0.3 5,975,419 - Pf6N2E2_5181 0.32 -0.8 5,975,419 - Pf6N2E2_5181 0.32 -1.3 5,975,419 - Pf6N2E2_5181 0.32 -1.3 5,975,419 - Pf6N2E2_5181 0.32 +0.3 5,975,437 + Pf6N2E2_5181 0.34 +0.3 5,975,627 + Pf6N2E2_5181 0.63 -0.3 5,975,628 - Pf6N2E2_5181 0.63 -0.2 5,975,631 + Pf6N2E2_5181 0.64 -0.4 5,975,631 + Pf6N2E2_5181 0.64 -0.2 5,975,632 - Pf6N2E2_5181 0.64 +1.3 5,975,632 - Pf6N2E2_5181 0.64 -0.6 5,975,647 - Pf6N2E2_5181 0.66 +0.3 5,975,656 - Pf6N2E2_5181 0.67 -0.6 5,975,665 + Pf6N2E2_5181 0.69 -1.9 5,975,665 + Pf6N2E2_5181 0.69 -1.1 5,975,666 - Pf6N2E2_5181 0.69 -0.7 5,975,692 - Pf6N2E2_5181 0.73 -0.6 5,975,749 + Pf6N2E2_5181 0.82 -0.3 5,975,749 + Pf6N2E2_5181 0.82 +0.4 5,975,750 - Pf6N2E2_5181 0.82 -2.4 5,976,028 + Pf6N2E2_5182 0.27 +0.6 5,976,029 - Pf6N2E2_5182 0.28 +0.3 5,976,029 - Pf6N2E2_5182 0.28 -1.9 5,976,029 - Pf6N2E2_5182 0.28 -0.2 5,976,029 - Pf6N2E2_5182 0.28 -1.7 5,976,031 - Pf6N2E2_5182 0.28 -0.7 5,976,060 + Pf6N2E2_5182 0.33 -1.0 5,976,061 - Pf6N2E2_5182 0.33 -2.7 5,976,234 + Pf6N2E2_5182 0.60 +0.8 5,976,234 + Pf6N2E2_5182 0.60 +0.7 5,976,234 + Pf6N2E2_5182 0.60 -1.2 5,976,234 + Pf6N2E2_5182 0.60 -1.9 5,976,234 + Pf6N2E2_5182 0.60 -0.7 5,976,235 - Pf6N2E2_5182 0.61 -0.5 5,976,258 + Pf6N2E2_5182 0.64 -0.9 5,976,258 + Pf6N2E2_5182 0.64 -0.2 5,976,258 + Pf6N2E2_5182 0.64 -0.4 5,976,258 + Pf6N2E2_5182 0.64 -1.2 5,976,258 + Pf6N2E2_5182 0.64 -0.3 5,976,259 - Pf6N2E2_5182 0.64 -1.0 5,976,259 - Pf6N2E2_5182 0.64 +0.1 5,976,259 - Pf6N2E2_5182 0.64 -1.0 5,976,259 - Pf6N2E2_5182 0.64 -0.9 5,976,259 - Pf6N2E2_5182 0.64 -3.6 5,976,259 - Pf6N2E2_5182 0.64 -0.2 5,976,259 - Pf6N2E2_5182 0.64 +0.8 5,976,259 - Pf6N2E2_5182 0.64 +0.5 5,976,259 - Pf6N2E2_5182 0.64 -3.2 5,976,259 - Pf6N2E2_5182 0.64 -1.2 5,976,262 + Pf6N2E2_5182 0.65 -1.3 5,976,262 + Pf6N2E2_5182 0.65 -1.0 5,976,263 - Pf6N2E2_5182 0.65 +0.4 5,976,582 - -1.7 5,976,582 - -0.7 5,976,659 + -0.3 5,976,765 - Pf6N2E2_5183 0.13 -1.7 5,976,765 - Pf6N2E2_5183 0.13 -0.6 5,976,765 - Pf6N2E2_5183 0.13 -0.0 5,976,851 - Pf6N2E2_5183 0.17 -1.2 5,976,854 + Pf6N2E2_5183 0.17 -0.0 5,976,854 + Pf6N2E2_5183 0.17 -3.2 5,976,854 + Pf6N2E2_5183 0.17 -1.3 5,976,854 + Pf6N2E2_5183 0.17 -0.9 5,976,854 + Pf6N2E2_5183 0.17 -0.2 5,976,854 + Pf6N2E2_5183 0.17 -0.7 5,976,854 + Pf6N2E2_5183 0.17 -1.5 5,976,854 + Pf6N2E2_5183 0.17 -0.0 5,976,854 + Pf6N2E2_5183 0.17 -0.1 5,976,854 + Pf6N2E2_5183 0.17 -0.2 5,976,854 + Pf6N2E2_5183 0.17 -2.5 5,976,854 + Pf6N2E2_5183 0.17 -0.3 5,976,854 + Pf6N2E2_5183 0.17 +1.6 5,976,854 + Pf6N2E2_5183 0.17 -3.2 5,976,854 + Pf6N2E2_5183 0.17 -0.8 5,976,855 - Pf6N2E2_5183 0.17 -0.7 5,976,855 - Pf6N2E2_5183 0.17 -2.1 5,976,855 - Pf6N2E2_5183 0.17 -1.0 5,976,855 - Pf6N2E2_5183 0.17 -2.8 5,976,855 - Pf6N2E2_5183 0.17 -0.1 5,976,855 - Pf6N2E2_5183 0.17 -0.5 5,976,855 - Pf6N2E2_5183 0.17 -0.5 5,976,855 - Pf6N2E2_5183 0.17 -1.0 5,976,855 - Pf6N2E2_5183 0.17 -1.8 5,976,855 - Pf6N2E2_5183 0.17 -0.5 5,976,855 - Pf6N2E2_5183 0.17 +0.6 5,976,855 - Pf6N2E2_5183 0.17 -1.1 5,976,855 - Pf6N2E2_5183 0.17 -0.5
Or see this region's nucleotide sequence