Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5152 and Pf6N2E2_5153 overlap by 4 nucleotides Pf6N2E2_5153 and Pf6N2E2_5154 are separated by 275 nucleotides Pf6N2E2_5154 and Pf6N2E2_5155 are separated by 3 nucleotides
Pf6N2E2_5152 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 5,939,268 to 5,940,128
_5152
Pf6N2E2_5153 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 5,940,125 to 5,940,925
_5153
Pf6N2E2_5154 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 5,941,201 to 5,941,686
_5154
Pf6N2E2_5155 - Poly(A) polymerase (EC 2.7.7.19), at 5,941,690 to 5,943,087
_5155
Position (kb)
5940
5941 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5939.147 kb on + strand at 5939.147 kb on + strand at 5939.148 kb on - strand at 5939.269 kb on + strand at 5939.270 kb on - strand at 5939.270 kb on - strand at 5939.516 kb on - strand, within Pf6N2E2_5152 at 5940.123 kb on - strand at 5940.123 kb on - strand at 5940.183 kb on + strand at 5940.183 kb on + strand at 5940.184 kb on - strand at 5940.184 kb on - strand at 5940.184 kb on - strand at 5940.184 kb on - strand at 5940.206 kb on - strand, within Pf6N2E2_5153 at 5940.291 kb on - strand, within Pf6N2E2_5153 at 5940.475 kb on + strand, within Pf6N2E2_5153 at 5940.475 kb on + strand, within Pf6N2E2_5153 at 5940.476 kb on - strand, within Pf6N2E2_5153 at 5940.476 kb on - strand, within Pf6N2E2_5153 at 5940.476 kb on - strand, within Pf6N2E2_5153 at 5940.477 kb on + strand, within Pf6N2E2_5153 at 5940.477 kb on + strand, within Pf6N2E2_5153 at 5940.477 kb on + strand, within Pf6N2E2_5153 at 5940.477 kb on + strand, within Pf6N2E2_5153 at 5940.478 kb on - strand, within Pf6N2E2_5153 at 5940.478 kb on - strand, within Pf6N2E2_5153 at 5940.478 kb on - strand, within Pf6N2E2_5153 at 5940.478 kb on - strand, within Pf6N2E2_5153 at 5940.657 kb on + strand, within Pf6N2E2_5153 at 5940.657 kb on + strand, within Pf6N2E2_5153 at 5940.658 kb on - strand, within Pf6N2E2_5153 at 5940.658 kb on - strand, within Pf6N2E2_5153 at 5940.726 kb on + strand, within Pf6N2E2_5153 at 5940.727 kb on - strand, within Pf6N2E2_5153 at 5940.853 kb on - strand at 5940.853 kb on - strand at 5940.853 kb on - strand at 5940.914 kb on - strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.916 kb on + strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.917 kb on - strand at 5940.940 kb on + strand at 5940.941 kb on - strand at 5940.955 kb on + strand at 5940.956 kb on - strand at 5940.965 kb on - strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.985 kb on + strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.986 kb on - strand at 5940.988 kb on - strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.002 kb on + strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.003 kb on - strand at 5941.017 kb on + strand at 5941.018 kb on - strand at 5941.018 kb on - strand at 5941.018 kb on - strand at 5941.069 kb on - strand at 5941.069 kb on - strand at 5941.069 kb on - strand at 5941.070 kb on + strand at 5941.070 kb on + strand at 5941.070 kb on + strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.071 kb on - strand at 5941.130 kb on + strand at 5941.206 kb on + strand at 5941.674 kb on - strand at 5941.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 5,939,147 + -0.7 5,939,147 + +0.5 5,939,148 - -0.4 5,939,269 + +1.1 5,939,270 - +0.8 5,939,270 - -1.1 5,939,516 - Pf6N2E2_5152 0.29 -3.5 5,940,123 - -0.7 5,940,123 - -1.2 5,940,183 + -1.0 5,940,183 + -2.5 5,940,184 - +0.2 5,940,184 - +0.5 5,940,184 - -0.3 5,940,184 - +1.1 5,940,206 - Pf6N2E2_5153 0.10 -0.4 5,940,291 - Pf6N2E2_5153 0.21 +0.7 5,940,475 + Pf6N2E2_5153 0.44 -0.2 5,940,475 + Pf6N2E2_5153 0.44 -2.3 5,940,476 - Pf6N2E2_5153 0.44 -0.7 5,940,476 - Pf6N2E2_5153 0.44 +0.6 5,940,476 - Pf6N2E2_5153 0.44 +0.2 5,940,477 + Pf6N2E2_5153 0.44 -3.5 5,940,477 + Pf6N2E2_5153 0.44 -1.7 5,940,477 + Pf6N2E2_5153 0.44 -0.3 5,940,477 + Pf6N2E2_5153 0.44 -0.1 5,940,478 - Pf6N2E2_5153 0.44 -0.7 5,940,478 - Pf6N2E2_5153 0.44 -1.2 5,940,478 - Pf6N2E2_5153 0.44 +0.4 5,940,478 - Pf6N2E2_5153 0.44 -2.2 5,940,657 + Pf6N2E2_5153 0.66 -0.9 5,940,657 + Pf6N2E2_5153 0.66 -0.8 5,940,658 - Pf6N2E2_5153 0.67 -0.2 5,940,658 - Pf6N2E2_5153 0.67 -0.5 5,940,726 + Pf6N2E2_5153 0.75 -0.7 5,940,727 - Pf6N2E2_5153 0.75 -0.7 5,940,853 - -0.3 5,940,853 - +0.5 5,940,853 - -0.7 5,940,914 - -0.3 5,940,916 + -2.1 5,940,916 + -1.7 5,940,916 + -2.3 5,940,916 + -3.1 5,940,917 - +1.2 5,940,917 - +0.1 5,940,917 - +0.1 5,940,917 - -0.0 5,940,940 + -0.7 5,940,941 - -0.5 5,940,955 + +0.5 5,940,956 - -0.2 5,940,965 - -0.9 5,940,985 + +0.3 5,940,985 + -2.4 5,940,985 + -1.7 5,940,985 + -0.8 5,940,985 + -1.5 5,940,985 + -0.2 5,940,985 + -0.3 5,940,986 - +0.0 5,940,986 - -0.5 5,940,986 - +1.0 5,940,986 - -1.0 5,940,986 - -0.4 5,940,986 - +0.3 5,940,986 - +0.9 5,940,986 - -0.9 5,940,986 - -0.8 5,940,986 - +0.2 5,940,986 - -0.6 5,940,986 - +0.6 5,940,988 - +0.2 5,941,002 + -0.3 5,941,002 + +0.7 5,941,002 + -0.5 5,941,002 + -3.1 5,941,003 - -1.1 5,941,003 - +0.4 5,941,003 - +0.3 5,941,003 - -0.0 5,941,017 + -0.9 5,941,018 - -0.6 5,941,018 - -0.4 5,941,018 - +2.3 5,941,069 - -0.9 5,941,069 - +0.1 5,941,069 - -0.2 5,941,070 + -1.6 5,941,070 + +0.6 5,941,070 + -0.4 5,941,071 - +0.0 5,941,071 - -1.7 5,941,071 - +0.3 5,941,071 - +0.1 5,941,130 + +0.7 5,941,206 + +0.4 5,941,674 - -0.3 5,941,674 - +0.8
Or see this region's nucleotide sequence