Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_50

Experiment: D-Cycloserine 0.12 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	D-Cycloserine 0.12 mg/ml
87,788	+				+1.0
87,788	+				+0.8
87,806	+				+1.1
87,806	+				-1.6
87,807	-				+0.8
87,906	+				+0.8
87,928	+				-0.1
87,929	-				+0.4
87,975	+				+0.1
88,034	-				+0.5
88,077	-		Pf6N2E2_6095	0.51	+2.0
88,077	-		Pf6N2E2_6095	0.51	+0.2
88,093	-		Pf6N2E2_6095	0.73	-1.2
88,093	-		Pf6N2E2_6095	0.73	-1.1
88,126	+				-0.6
88,233	+				+0.3
88,234	-				-2.2
88,285	+				-0.0
88,286	-				+0.1
88,286	-				-0.7
88,286	-				-0.7
88,286	-				+0.9
88,291	-				+0.7
88,291	-				+1.4
88,311	+				+0.3
88,312	-				-0.7
88,405	+		Pf6N2E2_49	0.25	-0.4
88,406	-		Pf6N2E2_49	0.25	+0.5
88,406	-		Pf6N2E2_49	0.25	-0.8
88,491	+		Pf6N2E2_49	0.63	+0.6
88,529	-		Pf6N2E2_49	0.79	+0.2
88,537	+		Pf6N2E2_49	0.83	+1.3
88,574	-				+0.7
88,624	-				-0.9
88,777	+				+0.3
88,800	+				-1.7
88,800	+				+0.2
88,800	+				+0.3
88,801	-				-1.3
88,876	+				-0.6
88,876	+				-0.8
88,876	+				+0.5
88,876	+				-1.2
88,876	+				-0.9
88,876	+				+0.8
88,876	+				+0.8
88,876	+				-0.5
88,876	+				-1.0
88,876	+				-2.1
88,877	-				-0.0
88,877	-				-1.2
88,877	-				-1.6
88,877	-				-0.7
88,877	-				+0.1
88,930	+		Pf6N2E2_50	0.12	-0.7
88,931	-		Pf6N2E2_50	0.12	-0.9
88,931	-		Pf6N2E2_50	0.12	-0.5
89,131	+		Pf6N2E2_50	0.29	-1.6
89,131	+		Pf6N2E2_50	0.29	-0.5
89,132	-		Pf6N2E2_50	0.29	+0.0
89,530	+		Pf6N2E2_50	0.62	-1.0
89,531	-		Pf6N2E2_50	0.62	-1.3
89,758	+		Pf6N2E2_50	0.81	-1.5
89,759	-		Pf6N2E2_50	0.81	+0.3
89,786	-		Pf6N2E2_50	0.83	-0.8
89,786	-		Pf6N2E2_50	0.83	-0.2
89,786	-		Pf6N2E2_50	0.83	+0.0
89,786	-		Pf6N2E2_50	0.83	+0.7
89,852	+		Pf6N2E2_50	0.89	-0.9
89,852	+		Pf6N2E2_50	0.89	-3.1
89,853	-		Pf6N2E2_50	0.89	+0.6
89,853	-		Pf6N2E2_50	0.89	-0.2
89,872	+				-0.2
89,872	+				-2.8
89,873	-				+0.2
89,903	+				-0.2
89,985	+				-0.6
89,986	-				-1.9
89,998	+				+0.1
89,999	-				-2.2
90,065	+		Pf6N2E2_51	0.33	-1.8
90,065	+		Pf6N2E2_51	0.33	-0.9
90,065	+		Pf6N2E2_51	0.33	+1.0
90,065	+		Pf6N2E2_51	0.33	-0.5
90,065	+		Pf6N2E2_51	0.33	-0.8
90,065	+		Pf6N2E2_51	0.33	-1.8
90,066	-		Pf6N2E2_51	0.34	-1.6
90,066	-		Pf6N2E2_51	0.34	-0.3
90,066	-		Pf6N2E2_51	0.34	-0.1
90,066	-		Pf6N2E2_51	0.34	-0.6
90,066	-		Pf6N2E2_51	0.34	+1.5
90,066	-		Pf6N2E2_51	0.34	-2.1
90,066	-		Pf6N2E2_51	0.34	+0.9
90,066	-		Pf6N2E2_51	0.34	-1.2
90,066	-		Pf6N2E2_51	0.34	-0.0
90,066	-		Pf6N2E2_51	0.34	-1.1
90,068	+		Pf6N2E2_51	0.35	+0.5
90,068	+		Pf6N2E2_51	0.35	-0.8
90,068	+		Pf6N2E2_51	0.35	-0.5
90,068	+		Pf6N2E2_51	0.35	+0.5
90,069	-		Pf6N2E2_51	0.35	-0.1
90,069	-		Pf6N2E2_51	0.35	-0.2
90,070	+		Pf6N2E2_51	0.36	+0.4
90,070	+		Pf6N2E2_51	0.36	-2.8
90,070	+		Pf6N2E2_51	0.36	-0.3
90,070	+		Pf6N2E2_51	0.36	-0.5
90,071	-		Pf6N2E2_51	0.36	-0.1
90,178	+				-1.2
90,178	+				-2.2
90,310	+				-0.2
90,310	+				-0.1
90,310	+				-0.3
90,311	-				-1.2
90,332	+				-0.4
90,332	+				-0.3
90,333	-				-1.4
90,532	+		Pf6N2E2_52	0.11	-0.8
90,532	+		Pf6N2E2_52	0.11	-0.4
90,574	+		Pf6N2E2_52	0.13	-1.5
90,574	+		Pf6N2E2_52	0.13	+0.1
90,575	-		Pf6N2E2_52	0.13	+0.5
90,586	+		Pf6N2E2_52	0.13	+1.5
90,587	-		Pf6N2E2_52	0.13	-0.8
90,595	+		Pf6N2E2_52	0.14	+0.1
90,595	+		Pf6N2E2_52	0.14	-1.2
90,598	+		Pf6N2E2_52	0.14	-0.9
90,599	-		Pf6N2E2_52	0.14	-1.4
90,599	-		Pf6N2E2_52	0.14	-1.6
90,599	-		Pf6N2E2_52	0.14	-1.4
90,613	+		Pf6N2E2_52	0.15	-1.6
90,614	-		Pf6N2E2_52	0.15	-0.4
90,614	-		Pf6N2E2_52	0.15	-1.8
90,748	+		Pf6N2E2_52	0.21	-1.1
90,748	+		Pf6N2E2_52	0.21	+0.3
90,749	-		Pf6N2E2_52	0.21	-1.0
90,772	+		Pf6N2E2_52	0.23	-1.0
90,773	-		Pf6N2E2_52	0.23	-1.3
90,773	-		Pf6N2E2_52	0.23	-0.4
90,773	-		Pf6N2E2_52	0.23	-1.7
90,853	+		Pf6N2E2_52	0.27	-2.3
90,853	+		Pf6N2E2_52	0.27	-0.6
90,853	+		Pf6N2E2_52	0.27	-3.2
90,854	-		Pf6N2E2_52	0.27	-0.7
90,854	-		Pf6N2E2_52	0.27	-1.6