Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_48 and Pf6N2E2_6095 are separated by 251 nucleotides Pf6N2E2_6095 and Pf6N2E2_49 are separated by 235 nucleotides Pf6N2E2_49 and Pf6N2E2_50 are separated by 212 nucleotides Pf6N2E2_50 and Pf6N2E2_51 are separated by 15 nucleotides Pf6N2E2_51 and Pf6N2E2_52 are separated by 121 nucleotides
Pf6N2E2_48 - FIG00955661: hypothetical protein, at 87,594 to 87,788
_48
Pf6N2E2_6095 - tRNA-Asn-GTT, at 88,040 to 88,112
_6095
Pf6N2E2_49 - FIG00967200: hypothetical protein, at 88,348 to 88,575
_49
Pf6N2E2_50 - Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57), at 88,788 to 89,984
_50
Pf6N2E2_51 - hypothetical protein, at 90,000 to 90,194
_51
Pf6N2E2_52 - Excinuclease ABC subunit B, at 90,316 to 92,331
_52
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 87.788 kb on + strand at 87.788 kb on + strand at 87.806 kb on + strand at 87.806 kb on + strand at 87.807 kb on - strand at 87.906 kb on + strand at 87.928 kb on + strand at 87.929 kb on - strand at 87.975 kb on + strand at 88.034 kb on - strand at 88.077 kb on - strand, within Pf6N2E2_6095 at 88.077 kb on - strand, within Pf6N2E2_6095 at 88.093 kb on - strand, within Pf6N2E2_6095 at 88.093 kb on - strand, within Pf6N2E2_6095 at 88.126 kb on + strand at 88.233 kb on + strand at 88.234 kb on - strand at 88.285 kb on + strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.286 kb on - strand at 88.291 kb on - strand at 88.291 kb on - strand at 88.311 kb on + strand at 88.312 kb on - strand at 88.405 kb on + strand, within Pf6N2E2_49 at 88.406 kb on - strand, within Pf6N2E2_49 at 88.406 kb on - strand, within Pf6N2E2_49 at 88.491 kb on + strand, within Pf6N2E2_49 at 88.529 kb on - strand, within Pf6N2E2_49 at 88.537 kb on + strand, within Pf6N2E2_49 at 88.574 kb on - strand at 88.624 kb on - strand at 88.777 kb on + strand at 88.800 kb on + strand at 88.800 kb on + strand at 88.800 kb on + strand at 88.801 kb on - strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.876 kb on + strand at 88.877 kb on - strand at 88.877 kb on - strand at 88.877 kb on - strand at 88.877 kb on - strand at 88.877 kb on - strand at 88.930 kb on + strand, within Pf6N2E2_50 at 88.931 kb on - strand, within Pf6N2E2_50 at 88.931 kb on - strand, within Pf6N2E2_50 at 89.131 kb on + strand, within Pf6N2E2_50 at 89.131 kb on + strand, within Pf6N2E2_50 at 89.132 kb on - strand, within Pf6N2E2_50 at 89.530 kb on + strand, within Pf6N2E2_50 at 89.531 kb on - strand, within Pf6N2E2_50 at 89.758 kb on + strand, within Pf6N2E2_50 at 89.759 kb on - strand, within Pf6N2E2_50 at 89.786 kb on - strand, within Pf6N2E2_50 at 89.786 kb on - strand, within Pf6N2E2_50 at 89.786 kb on - strand, within Pf6N2E2_50 at 89.786 kb on - strand, within Pf6N2E2_50 at 89.852 kb on + strand, within Pf6N2E2_50 at 89.852 kb on + strand, within Pf6N2E2_50 at 89.853 kb on - strand, within Pf6N2E2_50 at 89.853 kb on - strand, within Pf6N2E2_50 at 89.872 kb on + strand at 89.872 kb on + strand at 89.873 kb on - strand at 89.903 kb on + strand at 89.985 kb on + strand at 89.986 kb on - strand at 89.998 kb on + strand at 89.999 kb on - strand at 90.065 kb on + strand, within Pf6N2E2_51 at 90.065 kb on + strand, within Pf6N2E2_51 at 90.065 kb on + strand, within Pf6N2E2_51 at 90.065 kb on + strand, within Pf6N2E2_51 at 90.065 kb on + strand, within Pf6N2E2_51 at 90.065 kb on + strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.066 kb on - strand, within Pf6N2E2_51 at 90.068 kb on + strand, within Pf6N2E2_51 at 90.068 kb on + strand, within Pf6N2E2_51 at 90.068 kb on + strand, within Pf6N2E2_51 at 90.068 kb on + strand, within Pf6N2E2_51 at 90.069 kb on - strand, within Pf6N2E2_51 at 90.069 kb on - strand, within Pf6N2E2_51 at 90.070 kb on + strand, within Pf6N2E2_51 at 90.070 kb on + strand, within Pf6N2E2_51 at 90.070 kb on + strand, within Pf6N2E2_51 at 90.070 kb on + strand, within Pf6N2E2_51 at 90.071 kb on - strand, within Pf6N2E2_51 at 90.178 kb on + strand at 90.178 kb on + strand at 90.310 kb on + strand at 90.310 kb on + strand at 90.310 kb on + strand at 90.311 kb on - strand at 90.332 kb on + strand at 90.332 kb on + strand at 90.333 kb on - strand at 90.532 kb on + strand, within Pf6N2E2_52 at 90.532 kb on + strand, within Pf6N2E2_52 at 90.574 kb on + strand, within Pf6N2E2_52 at 90.574 kb on + strand, within Pf6N2E2_52 at 90.575 kb on - strand, within Pf6N2E2_52 at 90.586 kb on + strand, within Pf6N2E2_52 at 90.587 kb on - strand, within Pf6N2E2_52 at 90.595 kb on + strand, within Pf6N2E2_52 at 90.595 kb on + strand, within Pf6N2E2_52 at 90.598 kb on + strand, within Pf6N2E2_52 at 90.599 kb on - strand, within Pf6N2E2_52 at 90.599 kb on - strand, within Pf6N2E2_52 at 90.599 kb on - strand, within Pf6N2E2_52 at 90.613 kb on + strand, within Pf6N2E2_52 at 90.614 kb on - strand, within Pf6N2E2_52 at 90.614 kb on - strand, within Pf6N2E2_52 at 90.748 kb on + strand, within Pf6N2E2_52 at 90.748 kb on + strand, within Pf6N2E2_52 at 90.749 kb on - strand, within Pf6N2E2_52 at 90.772 kb on + strand, within Pf6N2E2_52 at 90.773 kb on - strand, within Pf6N2E2_52 at 90.773 kb on - strand, within Pf6N2E2_52 at 90.773 kb on - strand, within Pf6N2E2_52 at 90.853 kb on + strand, within Pf6N2E2_52 at 90.853 kb on + strand, within Pf6N2E2_52 at 90.853 kb on + strand, within Pf6N2E2_52 at 90.854 kb on - strand, within Pf6N2E2_52 at 90.854 kb on - strand, within Pf6N2E2_52
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 87,788 + +1.0 87,788 + +0.8 87,806 + +1.1 87,806 + -1.6 87,807 - +0.8 87,906 + +0.8 87,928 + -0.1 87,929 - +0.4 87,975 + +0.1 88,034 - +0.5 88,077 - Pf6N2E2_6095 0.51 +2.0 88,077 - Pf6N2E2_6095 0.51 +0.2 88,093 - Pf6N2E2_6095 0.73 -1.2 88,093 - Pf6N2E2_6095 0.73 -1.1 88,126 + -0.6 88,233 + +0.3 88,234 - -2.2 88,285 + -0.0 88,286 - +0.1 88,286 - -0.7 88,286 - -0.7 88,286 - +0.9 88,291 - +0.7 88,291 - +1.4 88,311 + +0.3 88,312 - -0.7 88,405 + Pf6N2E2_49 0.25 -0.4 88,406 - Pf6N2E2_49 0.25 +0.5 88,406 - Pf6N2E2_49 0.25 -0.8 88,491 + Pf6N2E2_49 0.63 +0.6 88,529 - Pf6N2E2_49 0.79 +0.2 88,537 + Pf6N2E2_49 0.83 +1.3 88,574 - +0.7 88,624 - -0.9 88,777 + +0.3 88,800 + -1.7 88,800 + +0.2 88,800 + +0.3 88,801 - -1.3 88,876 + -0.6 88,876 + -0.8 88,876 + +0.5 88,876 + -1.2 88,876 + -0.9 88,876 + +0.8 88,876 + +0.8 88,876 + -0.5 88,876 + -1.0 88,876 + -2.1 88,877 - -0.0 88,877 - -1.2 88,877 - -1.6 88,877 - -0.7 88,877 - +0.1 88,930 + Pf6N2E2_50 0.12 -0.7 88,931 - Pf6N2E2_50 0.12 -0.9 88,931 - Pf6N2E2_50 0.12 -0.5 89,131 + Pf6N2E2_50 0.29 -1.6 89,131 + Pf6N2E2_50 0.29 -0.5 89,132 - Pf6N2E2_50 0.29 +0.0 89,530 + Pf6N2E2_50 0.62 -1.0 89,531 - Pf6N2E2_50 0.62 -1.3 89,758 + Pf6N2E2_50 0.81 -1.5 89,759 - Pf6N2E2_50 0.81 +0.3 89,786 - Pf6N2E2_50 0.83 -0.8 89,786 - Pf6N2E2_50 0.83 -0.2 89,786 - Pf6N2E2_50 0.83 +0.0 89,786 - Pf6N2E2_50 0.83 +0.7 89,852 + Pf6N2E2_50 0.89 -0.9 89,852 + Pf6N2E2_50 0.89 -3.1 89,853 - Pf6N2E2_50 0.89 +0.6 89,853 - Pf6N2E2_50 0.89 -0.2 89,872 + -0.2 89,872 + -2.8 89,873 - +0.2 89,903 + -0.2 89,985 + -0.6 89,986 - -1.9 89,998 + +0.1 89,999 - -2.2 90,065 + Pf6N2E2_51 0.33 -1.8 90,065 + Pf6N2E2_51 0.33 -0.9 90,065 + Pf6N2E2_51 0.33 +1.0 90,065 + Pf6N2E2_51 0.33 -0.5 90,065 + Pf6N2E2_51 0.33 -0.8 90,065 + Pf6N2E2_51 0.33 -1.8 90,066 - Pf6N2E2_51 0.34 -1.6 90,066 - Pf6N2E2_51 0.34 -0.3 90,066 - Pf6N2E2_51 0.34 -0.1 90,066 - Pf6N2E2_51 0.34 -0.6 90,066 - Pf6N2E2_51 0.34 +1.5 90,066 - Pf6N2E2_51 0.34 -2.1 90,066 - Pf6N2E2_51 0.34 +0.9 90,066 - Pf6N2E2_51 0.34 -1.2 90,066 - Pf6N2E2_51 0.34 -0.0 90,066 - Pf6N2E2_51 0.34 -1.1 90,068 + Pf6N2E2_51 0.35 +0.5 90,068 + Pf6N2E2_51 0.35 -0.8 90,068 + Pf6N2E2_51 0.35 -0.5 90,068 + Pf6N2E2_51 0.35 +0.5 90,069 - Pf6N2E2_51 0.35 -0.1 90,069 - Pf6N2E2_51 0.35 -0.2 90,070 + Pf6N2E2_51 0.36 +0.4 90,070 + Pf6N2E2_51 0.36 -2.8 90,070 + Pf6N2E2_51 0.36 -0.3 90,070 + Pf6N2E2_51 0.36 -0.5 90,071 - Pf6N2E2_51 0.36 -0.1 90,178 + -1.2 90,178 + -2.2 90,310 + -0.2 90,310 + -0.1 90,310 + -0.3 90,311 - -1.2 90,332 + -0.4 90,332 + -0.3 90,333 - -1.4 90,532 + Pf6N2E2_52 0.11 -0.8 90,532 + Pf6N2E2_52 0.11 -0.4 90,574 + Pf6N2E2_52 0.13 -1.5 90,574 + Pf6N2E2_52 0.13 +0.1 90,575 - Pf6N2E2_52 0.13 +0.5 90,586 + Pf6N2E2_52 0.13 +1.5 90,587 - Pf6N2E2_52 0.13 -0.8 90,595 + Pf6N2E2_52 0.14 +0.1 90,595 + Pf6N2E2_52 0.14 -1.2 90,598 + Pf6N2E2_52 0.14 -0.9 90,599 - Pf6N2E2_52 0.14 -1.4 90,599 - Pf6N2E2_52 0.14 -1.6 90,599 - Pf6N2E2_52 0.14 -1.4 90,613 + Pf6N2E2_52 0.15 -1.6 90,614 - Pf6N2E2_52 0.15 -0.4 90,614 - Pf6N2E2_52 0.15 -1.8 90,748 + Pf6N2E2_52 0.21 -1.1 90,748 + Pf6N2E2_52 0.21 +0.3 90,749 - Pf6N2E2_52 0.21 -1.0 90,772 + Pf6N2E2_52 0.23 -1.0 90,773 - Pf6N2E2_52 0.23 -1.3 90,773 - Pf6N2E2_52 0.23 -0.4 90,773 - Pf6N2E2_52 0.23 -1.7 90,853 + Pf6N2E2_52 0.27 -2.3 90,853 + Pf6N2E2_52 0.27 -0.6 90,853 + Pf6N2E2_52 0.27 -3.2 90,854 - Pf6N2E2_52 0.27 -0.7 90,854 - Pf6N2E2_52 0.27 -1.6
Or see this region's nucleotide sequence