Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4918

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4916 and Pf6N2E2_4917 overlap by 4 nucleotidesPf6N2E2_4917 and Pf6N2E2_4918 overlap by 2 nucleotidesPf6N2E2_4918 and Pf6N2E2_4919 overlap by 4 nucleotides Pf6N2E2_4916 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 5,671,618 to 5,672,274 _4916 Pf6N2E2_4917 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,672,271 to 5,672,762 _4917 Pf6N2E2_4918 - Ribonucleotide reductase transcriptional regulator NrdR, at 5,672,761 to 5,673,327 _4918 Pf6N2E2_4919 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 5,673,324 to 5,674,457 _4919 Position (kb) 5672 5673 5674Strain fitness (log2 ratio) -2 -1 0 1 2at 5671.814 kb on + strand, within Pf6N2E2_4916at 5671.815 kb on - strand, within Pf6N2E2_4916at 5672.021 kb on - strand, within Pf6N2E2_4916at 5672.060 kb on + strand, within Pf6N2E2_4916at 5672.061 kb on - strand, within Pf6N2E2_4916at 5672.102 kb on + strand, within Pf6N2E2_4916at 5672.478 kb on + strand, within Pf6N2E2_4917at 5672.478 kb on + strand, within Pf6N2E2_4917at 5672.478 kb on + strand, within Pf6N2E2_4917at 5672.479 kb on - strand, within Pf6N2E2_4917at 5672.479 kb on - strand, within Pf6N2E2_4917at 5672.596 kb on - strand, within Pf6N2E2_4917at 5672.596 kb on - strand, within Pf6N2E2_4917at 5672.775 kb on - strandat 5672.786 kb on + strandat 5672.786 kb on + strandat 5672.786 kb on + strandat 5672.787 kb on - strandat 5672.787 kb on - strandat 5672.787 kb on - strandat 5672.787 kb on - strandat 5672.788 kb on + strandat 5672.793 kb on + strandat 5672.794 kb on - strandat 5672.800 kb on + strandat 5672.800 kb on + strandat 5672.800 kb on + strandat 5672.801 kb on - strandat 5672.801 kb on - strandat 5672.801 kb on - strandat 5673.232 kb on + strand, within Pf6N2E2_4918at 5673.232 kb on + strand, within Pf6N2E2_4918at 5673.253 kb on + strand, within Pf6N2E2_4918at 5673.363 kb on + strandat 5673.363 kb on + strandat 5673.363 kb on + strandat 5673.363 kb on + strandat 5673.363 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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5,671,814 + Pf6N2E2_4916 0.30 +0.0
5,671,815 - Pf6N2E2_4916 0.30 -0.1
5,672,021 - Pf6N2E2_4916 0.61 +0.8
5,672,060 + Pf6N2E2_4916 0.67 -0.0
5,672,061 - Pf6N2E2_4916 0.67 +0.6
5,672,102 + Pf6N2E2_4916 0.74 -0.9
5,672,478 + Pf6N2E2_4917 0.42 +0.3
5,672,478 + Pf6N2E2_4917 0.42 +1.0
5,672,478 + Pf6N2E2_4917 0.42 +0.9
5,672,479 - Pf6N2E2_4917 0.42 +0.7
5,672,479 - Pf6N2E2_4917 0.42 -0.0
5,672,596 - Pf6N2E2_4917 0.66 -2.4
5,672,596 - Pf6N2E2_4917 0.66 -0.4
5,672,775 - +0.2
5,672,786 + +0.9
5,672,786 + -0.2
5,672,786 + +0.5
5,672,787 - -0.4
5,672,787 - +0.1
5,672,787 - -0.5
5,672,787 - -0.3
5,672,788 + -0.1
5,672,793 + +0.5
5,672,794 - +1.0
5,672,800 + +0.4
5,672,800 + -0.2
5,672,800 + -1.6
5,672,801 - +2.0
5,672,801 - +0.7
5,672,801 - +0.8
5,673,232 + Pf6N2E2_4918 0.83 -1.5
5,673,232 + Pf6N2E2_4918 0.83 -1.1
5,673,253 + Pf6N2E2_4918 0.87 -1.8
5,673,363 + +0.5
5,673,363 + +1.0
5,673,363 + +0.4
5,673,363 + -0.4
5,673,363 + +0.5

Or see this region's nucleotide sequence