Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4916 and Pf6N2E2_4917 overlap by 4 nucleotides Pf6N2E2_4917 and Pf6N2E2_4918 overlap by 2 nucleotides Pf6N2E2_4918 and Pf6N2E2_4919 overlap by 4 nucleotides
Pf6N2E2_4916 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 5,671,618 to 5,672,274
_4916
Pf6N2E2_4917 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,672,271 to 5,672,762
_4917
Pf6N2E2_4918 - Ribonucleotide reductase transcriptional regulator NrdR, at 5,672,761 to 5,673,327
_4918
Pf6N2E2_4919 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 5,673,324 to 5,674,457
_4919
Position (kb)
5672
5673
5674 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5671.814 kb on + strand, within Pf6N2E2_4916 at 5671.815 kb on - strand, within Pf6N2E2_4916 at 5672.021 kb on - strand, within Pf6N2E2_4916 at 5672.060 kb on + strand, within Pf6N2E2_4916 at 5672.061 kb on - strand, within Pf6N2E2_4916 at 5672.102 kb on + strand, within Pf6N2E2_4916 at 5672.478 kb on + strand, within Pf6N2E2_4917 at 5672.478 kb on + strand, within Pf6N2E2_4917 at 5672.478 kb on + strand, within Pf6N2E2_4917 at 5672.479 kb on - strand, within Pf6N2E2_4917 at 5672.479 kb on - strand, within Pf6N2E2_4917 at 5672.596 kb on - strand, within Pf6N2E2_4917 at 5672.596 kb on - strand, within Pf6N2E2_4917 at 5672.775 kb on - strand at 5672.786 kb on + strand at 5672.786 kb on + strand at 5672.786 kb on + strand at 5672.787 kb on - strand at 5672.787 kb on - strand at 5672.787 kb on - strand at 5672.787 kb on - strand at 5672.788 kb on + strand at 5672.793 kb on + strand at 5672.794 kb on - strand at 5672.800 kb on + strand at 5672.800 kb on + strand at 5672.800 kb on + strand at 5672.801 kb on - strand at 5672.801 kb on - strand at 5672.801 kb on - strand at 5673.232 kb on + strand, within Pf6N2E2_4918 at 5673.232 kb on + strand, within Pf6N2E2_4918 at 5673.253 kb on + strand, within Pf6N2E2_4918 at 5673.363 kb on + strand at 5673.363 kb on + strand at 5673.363 kb on + strand at 5673.363 kb on + strand at 5673.363 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 5,671,814 + Pf6N2E2_4916 0.30 +0.0 5,671,815 - Pf6N2E2_4916 0.30 -0.1 5,672,021 - Pf6N2E2_4916 0.61 +0.8 5,672,060 + Pf6N2E2_4916 0.67 -0.0 5,672,061 - Pf6N2E2_4916 0.67 +0.6 5,672,102 + Pf6N2E2_4916 0.74 -0.9 5,672,478 + Pf6N2E2_4917 0.42 +0.3 5,672,478 + Pf6N2E2_4917 0.42 +1.0 5,672,478 + Pf6N2E2_4917 0.42 +0.9 5,672,479 - Pf6N2E2_4917 0.42 +0.7 5,672,479 - Pf6N2E2_4917 0.42 -0.0 5,672,596 - Pf6N2E2_4917 0.66 -2.4 5,672,596 - Pf6N2E2_4917 0.66 -0.4 5,672,775 - +0.2 5,672,786 + +0.9 5,672,786 + -0.2 5,672,786 + +0.5 5,672,787 - -0.4 5,672,787 - +0.1 5,672,787 - -0.5 5,672,787 - -0.3 5,672,788 + -0.1 5,672,793 + +0.5 5,672,794 - +1.0 5,672,800 + +0.4 5,672,800 + -0.2 5,672,800 + -1.6 5,672,801 - +2.0 5,672,801 - +0.7 5,672,801 - +0.8 5,673,232 + Pf6N2E2_4918 0.83 -1.5 5,673,232 + Pf6N2E2_4918 0.83 -1.1 5,673,253 + Pf6N2E2_4918 0.87 -1.8 5,673,363 + +0.5 5,673,363 + +1.0 5,673,363 + +0.4 5,673,363 + -0.4 5,673,363 + +0.5
Or see this region's nucleotide sequence