Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4749

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4747 and Pf6N2E2_4748 overlap by 4 nucleotidesPf6N2E2_4748 and Pf6N2E2_4749 are separated by 239 nucleotidesPf6N2E2_4749 and Pf6N2E2_4750 are separated by 32 nucleotides Pf6N2E2_4747 - Transmembrane sensor, at 5,483,604 to 5,484,578 _4747 Pf6N2E2_4748 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,484,575 to 5,485,024 _4748 Pf6N2E2_4749 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 5,485,264 to 5,485,935 _4749 Pf6N2E2_4750 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 5,485,968 to 5,487,908 _4750 Position (kb) 5485 5486Strain fitness (log2 ratio) -1 0 1 2at 5484.266 kb on - strand, within Pf6N2E2_4747at 5484.591 kb on + strandat 5484.591 kb on + strandat 5484.591 kb on + strandat 5484.592 kb on - strandat 5484.592 kb on - strandat 5484.603 kb on + strandat 5484.603 kb on + strandat 5484.603 kb on + strandat 5484.603 kb on + strandat 5484.603 kb on + strandat 5484.603 kb on + strandat 5484.603 kb on + strandat 5484.603 kb on + strandat 5484.604 kb on - strandat 5484.630 kb on + strand, within Pf6N2E2_4748at 5484.631 kb on - strand, within Pf6N2E2_4748at 5484.631 kb on - strand, within Pf6N2E2_4748at 5484.639 kb on - strand, within Pf6N2E2_4748at 5484.704 kb on - strand, within Pf6N2E2_4748at 5484.705 kb on + strand, within Pf6N2E2_4748at 5484.705 kb on + strand, within Pf6N2E2_4748at 5484.705 kb on + strand, within Pf6N2E2_4748at 5484.705 kb on + strand, within Pf6N2E2_4748at 5484.706 kb on - strand, within Pf6N2E2_4748at 5484.706 kb on - strand, within Pf6N2E2_4748at 5484.706 kb on - strand, within Pf6N2E2_4748at 5484.840 kb on + strand, within Pf6N2E2_4748at 5484.840 kb on + strand, within Pf6N2E2_4748at 5484.840 kb on + strand, within Pf6N2E2_4748at 5484.840 kb on + strand, within Pf6N2E2_4748at 5484.841 kb on - strand, within Pf6N2E2_4748at 5484.841 kb on - strand, within Pf6N2E2_4748at 5484.871 kb on - strand, within Pf6N2E2_4748at 5485.025 kb on + strandat 5485.047 kb on + strandat 5485.047 kb on + strandat 5485.047 kb on + strandat 5485.047 kb on + strandat 5485.048 kb on - strandat 5485.048 kb on - strandat 5485.048 kb on - strandat 5485.049 kb on + strandat 5485.189 kb on + strandat 5485.240 kb on + strandat 5485.240 kb on + strandat 5485.240 kb on + strandat 5485.544 kb on + strand, within Pf6N2E2_4749at 5485.544 kb on + strand, within Pf6N2E2_4749at 5485.544 kb on + strand, within Pf6N2E2_4749at 5485.544 kb on + strand, within Pf6N2E2_4749at 5485.545 kb on - strand, within Pf6N2E2_4749at 5485.545 kb on - strand, within Pf6N2E2_4749at 5485.978 kb on + strandat 5486.084 kb on - strandat 5486.146 kb on + strandat 5486.146 kb on + strandat 5486.146 kb on + strandat 5486.147 kb on - strandat 5486.158 kb on + strandat 5486.159 kb on - strandat 5486.159 kb on - strandat 5486.222 kb on + strand, within Pf6N2E2_4750at 5486.222 kb on + strand, within Pf6N2E2_4750at 5486.222 kb on + strand, within Pf6N2E2_4750at 5486.223 kb on - strand, within Pf6N2E2_4750at 5486.223 kb on - strand, within Pf6N2E2_4750at 5486.223 kb on - strand, within Pf6N2E2_4750at 5486.223 kb on - strand, within Pf6N2E2_4750at 5486.494 kb on + strand, within Pf6N2E2_4750at 5486.494 kb on + strand, within Pf6N2E2_4750at 5486.494 kb on + strand, within Pf6N2E2_4750at 5486.494 kb on + strand, within Pf6N2E2_4750at 5486.494 kb on + strand, within Pf6N2E2_4750at 5486.494 kb on + strand, within Pf6N2E2_4750at 5486.494 kb on + strand, within Pf6N2E2_4750at 5486.495 kb on - strand, within Pf6N2E2_4750at 5486.495 kb on - strand, within Pf6N2E2_4750at 5486.495 kb on - strand, within Pf6N2E2_4750at 5486.495 kb on - strand, within Pf6N2E2_4750at 5486.670 kb on + strand, within Pf6N2E2_4750at 5486.832 kb on + strand, within Pf6N2E2_4750at 5486.832 kb on + strand, within Pf6N2E2_4750at 5486.832 kb on + strand, within Pf6N2E2_4750at 5486.832 kb on + strand, within Pf6N2E2_4750at 5486.833 kb on - strand, within Pf6N2E2_4750at 5486.844 kb on + strand, within Pf6N2E2_4750at 5486.844 kb on + strand, within Pf6N2E2_4750at 5486.844 kb on + strand, within Pf6N2E2_4750at 5486.845 kb on - strandat 5486.845 kb on - strand, within Pf6N2E2_4750at 5486.845 kb on - strand, within Pf6N2E2_4750at 5486.845 kb on - strand, within Pf6N2E2_4750

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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5,484,266 - Pf6N2E2_4747 0.68 -0.6
5,484,591 + +0.5
5,484,591 + -0.3
5,484,591 + +0.0
5,484,592 - -0.1
5,484,592 - -1.4
5,484,603 + -0.8
5,484,603 + +0.7
5,484,603 + -0.0
5,484,603 + +0.2
5,484,603 + +0.2
5,484,603 + -0.6
5,484,603 + +0.1
5,484,603 + +0.7
5,484,604 - -0.3
5,484,630 + Pf6N2E2_4748 0.12 -0.5
5,484,631 - Pf6N2E2_4748 0.12 +0.3
5,484,631 - Pf6N2E2_4748 0.12 -0.3
5,484,639 - Pf6N2E2_4748 0.14 +0.0
5,484,704 - Pf6N2E2_4748 0.29 +0.6
5,484,705 + Pf6N2E2_4748 0.29 +0.3
5,484,705 + Pf6N2E2_4748 0.29 +0.5
5,484,705 + Pf6N2E2_4748 0.29 +0.7
5,484,705 + Pf6N2E2_4748 0.29 +0.4
5,484,706 - Pf6N2E2_4748 0.29 -0.3
5,484,706 - Pf6N2E2_4748 0.29 -0.3
5,484,706 - Pf6N2E2_4748 0.29 -0.2
5,484,840 + Pf6N2E2_4748 0.59 +0.5
5,484,840 + Pf6N2E2_4748 0.59 +0.6
5,484,840 + Pf6N2E2_4748 0.59 +0.2
5,484,840 + Pf6N2E2_4748 0.59 -0.5
5,484,841 - Pf6N2E2_4748 0.59 +0.3
5,484,841 - Pf6N2E2_4748 0.59 -0.4
5,484,871 - Pf6N2E2_4748 0.66 +2.0
5,485,025 + +0.8
5,485,047 + +0.6
5,485,047 + -0.7
5,485,047 + -0.9
5,485,047 + -0.8
5,485,048 - +0.5
5,485,048 - +0.9
5,485,048 - -1.3
5,485,049 + +1.4
5,485,189 + +1.9
5,485,240 + +0.4
5,485,240 + +0.2
5,485,240 + +0.9
5,485,544 + Pf6N2E2_4749 0.42 +0.2
5,485,544 + Pf6N2E2_4749 0.42 -0.4
5,485,544 + Pf6N2E2_4749 0.42 +0.2
5,485,544 + Pf6N2E2_4749 0.42 -0.6
5,485,545 - Pf6N2E2_4749 0.42 -1.0
5,485,545 - Pf6N2E2_4749 0.42 +0.4
5,485,978 + +0.8
5,486,084 - -1.1
5,486,146 + +0.6
5,486,146 + +0.6
5,486,146 + -0.1
5,486,147 - +0.9
5,486,158 + -0.3
5,486,159 - +0.5
5,486,159 - +0.3
5,486,222 + Pf6N2E2_4750 0.13 +0.1
5,486,222 + Pf6N2E2_4750 0.13 -0.7
5,486,222 + Pf6N2E2_4750 0.13 -0.3
5,486,223 - Pf6N2E2_4750 0.13 -0.5
5,486,223 - Pf6N2E2_4750 0.13 +0.2
5,486,223 - Pf6N2E2_4750 0.13 +0.0
5,486,223 - Pf6N2E2_4750 0.13 -1.0
5,486,494 + Pf6N2E2_4750 0.27 -1.0
5,486,494 + Pf6N2E2_4750 0.27 +2.2
5,486,494 + Pf6N2E2_4750 0.27 -1.2
5,486,494 + Pf6N2E2_4750 0.27 -0.2
5,486,494 + Pf6N2E2_4750 0.27 -0.4
5,486,494 + Pf6N2E2_4750 0.27 +0.2
5,486,494 + Pf6N2E2_4750 0.27 +1.1
5,486,495 - Pf6N2E2_4750 0.27 +0.6
5,486,495 - Pf6N2E2_4750 0.27 +0.4
5,486,495 - Pf6N2E2_4750 0.27 +0.1
5,486,495 - Pf6N2E2_4750 0.27 -0.7
5,486,670 + Pf6N2E2_4750 0.36 +0.9
5,486,832 + Pf6N2E2_4750 0.45 +1.0
5,486,832 + Pf6N2E2_4750 0.45 +0.4
5,486,832 + Pf6N2E2_4750 0.45 +0.4
5,486,832 + Pf6N2E2_4750 0.45 +1.0
5,486,833 - Pf6N2E2_4750 0.45 +0.4
5,486,844 + Pf6N2E2_4750 0.45 +0.0
5,486,844 + Pf6N2E2_4750 0.45 -0.7
5,486,844 + Pf6N2E2_4750 0.45 -0.9
5,486,845 - +0.4
5,486,845 - Pf6N2E2_4750 0.45 +0.4
5,486,845 - Pf6N2E2_4750 0.45 -1.6
5,486,845 - Pf6N2E2_4750 0.45 +0.3

Or see this region's nucleotide sequence