Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4612 and Pf6N2E2_4613 are separated by 152 nucleotides Pf6N2E2_4613 and Pf6N2E2_4614 are separated by 48 nucleotides Pf6N2E2_4614 and Pf6N2E2_4615 are separated by 10 nucleotides Pf6N2E2_4615 and Pf6N2E2_4616 are separated by 158 nucleotides
Pf6N2E2_4612 - Glutathione synthetase (EC 6.3.2.3), at 5,336,898 to 5,337,851
_4612
Pf6N2E2_4613 - twitching motility protein PilG, at 5,338,004 to 5,338,504
_4613
Pf6N2E2_4614 - twitching motility protein PilH, at 5,338,553 to 5,338,918
_4614
Pf6N2E2_4615 - type IV pili signal transduction protein PilI, at 5,338,929 to 5,339,468
_4615
Pf6N2E2_4616 - type IV pilus biogenesis protein PilJ, at 5,339,627 to 5,341,690
_4616
Position (kb)
5338
5339 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.792 kb on - strand at 5337.800 kb on + strand at 5337.803 kb on - strand at 5337.843 kb on + strand at 5337.844 kb on - strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.984 kb on + strand at 5338.047 kb on + strand at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.596 kb on - strand, within Pf6N2E2_4614 at 5338.853 kb on + strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5339.067 kb on + strand, within Pf6N2E2_4615 at 5339.068 kb on - strand, within Pf6N2E2_4615 at 5339.528 kb on + strand at 5339.528 kb on + strand at 5339.612 kb on + strand at 5339.613 kb on - strand at 5339.742 kb on - strand at 5339.762 kb on + strand at 5339.763 kb on - strand at 5339.774 kb on + strand at 5339.774 kb on + strand at 5339.774 kb on + strand at 5339.775 kb on - strand at 5339.775 kb on - strand at 5339.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 5,337,559 + Pf6N2E2_4612 0.69 +0.8 5,337,559 + Pf6N2E2_4612 0.69 -1.4 5,337,740 - Pf6N2E2_4612 0.88 -0.8 5,337,740 - Pf6N2E2_4612 0.88 -1.7 5,337,792 - -1.2 5,337,800 + -2.2 5,337,803 - -3.3 5,337,843 + -0.9 5,337,844 - -0.8 5,337,882 + -0.2 5,337,882 + -0.3 5,337,882 + -0.6 5,337,882 + -0.6 5,337,882 + -0.3 5,337,883 - -0.1 5,337,883 - -0.5 5,337,883 - +0.5 5,337,883 - +0.5 5,337,883 - +1.0 5,337,883 - -0.3 5,337,883 - +0.2 5,337,883 - -0.6 5,337,883 - +1.5 5,337,984 + +1.1 5,338,047 + +1.1 5,338,595 + Pf6N2E2_4614 0.11 -0.5 5,338,595 + Pf6N2E2_4614 0.11 -0.8 5,338,595 + Pf6N2E2_4614 0.11 -0.5 5,338,596 - Pf6N2E2_4614 0.12 -0.7 5,338,853 + Pf6N2E2_4614 0.82 -0.5 5,338,854 - Pf6N2E2_4614 0.82 +0.3 5,338,854 - Pf6N2E2_4614 0.82 +1.9 5,338,854 - Pf6N2E2_4614 0.82 +1.4 5,338,854 - Pf6N2E2_4614 0.82 -0.7 5,338,854 - Pf6N2E2_4614 0.82 +0.5 5,338,854 - Pf6N2E2_4614 0.82 -0.6 5,339,067 + Pf6N2E2_4615 0.26 -0.4 5,339,068 - Pf6N2E2_4615 0.26 -0.2 5,339,528 + +0.8 5,339,528 + -0.1 5,339,612 + +0.7 5,339,613 - +0.5 5,339,742 - -0.4 5,339,762 + -0.1 5,339,763 - +0.8 5,339,774 + +0.2 5,339,774 + +0.2 5,339,774 + -0.5 5,339,775 - -0.3 5,339,775 - +1.3 5,339,775 - +0.2
Or see this region's nucleotide sequence