Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4610 and Pf6N2E2_4611 are separated by 46 nucleotides Pf6N2E2_4611 and Pf6N2E2_4612 are separated by 77 nucleotides
Pf6N2E2_4610 - Mobile element protein, at 5,334,708 to 5,335,877
_4610
Pf6N2E2_4611 - Ferric siderophore transport system, periplasmic binding protein TonB, at 5,335,924 to 5,336,820
_4611
Pf6N2E2_4612 - Glutathione synthetase (EC 6.3.2.3), at 5,336,898 to 5,337,851
_4612
Position (kb) 5335
5336
5337 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5335.862 kb on + strand at 5335.896 kb on + strand at 5335.896 kb on + strand at 5335.896 kb on + strand at 5335.911 kb on + strand at 5335.911 kb on + strand at 5335.912 kb on - strand at 5335.912 kb on - strand at 5335.912 kb on - strand at 5336.083 kb on + strand, within Pf6N2E2_4611 at 5336.083 kb on + strand, within Pf6N2E2_4611 at 5336.099 kb on + strand, within Pf6N2E2_4611 at 5336.099 kb on + strand, within Pf6N2E2_4611 at 5336.100 kb on - strand, within Pf6N2E2_4611 at 5336.148 kb on - strand, within Pf6N2E2_4611 at 5336.291 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.863 kb on + strand at 5337.188 kb on - strand, within Pf6N2E2_4612 at 5337.188 kb on - strand, within Pf6N2E2_4612 at 5337.250 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.792 kb on - strand at 5337.800 kb on + strand at 5337.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 5,335,862 + +2.1 5,335,896 + -0.6 5,335,896 + +2.9 5,335,896 + +0.2 5,335,911 + +0.7 5,335,911 + -0.0 5,335,912 - +0.4 5,335,912 - -0.0 5,335,912 - +0.0 5,336,083 + Pf6N2E2_4611 0.18 -2.7 5,336,083 + Pf6N2E2_4611 0.18 -1.1 5,336,099 + Pf6N2E2_4611 0.20 -0.4 5,336,099 + Pf6N2E2_4611 0.20 -1.1 5,336,100 - Pf6N2E2_4611 0.20 +1.9 5,336,148 - Pf6N2E2_4611 0.25 -1.1 5,336,291 + Pf6N2E2_4611 0.41 -0.5 5,336,517 + Pf6N2E2_4611 0.66 -2.0 5,336,517 + Pf6N2E2_4611 0.66 -0.1 5,336,517 + Pf6N2E2_4611 0.66 -0.0 5,336,517 + Pf6N2E2_4611 0.66 -1.4 5,336,517 + Pf6N2E2_4611 0.66 -0.3 5,336,517 + Pf6N2E2_4611 0.66 +0.1 5,336,863 + -0.4 5,337,188 - Pf6N2E2_4612 0.30 +0.3 5,337,188 - Pf6N2E2_4612 0.30 +0.4 5,337,250 + Pf6N2E2_4612 0.37 +0.0 5,337,550 + Pf6N2E2_4612 0.68 +1.2 5,337,550 + Pf6N2E2_4612 0.68 +1.1 5,337,550 + Pf6N2E2_4612 0.68 +0.1 5,337,550 + Pf6N2E2_4612 0.68 -0.4 5,337,550 + Pf6N2E2_4612 0.68 -0.0 5,337,551 - Pf6N2E2_4612 0.68 -1.2 5,337,551 - Pf6N2E2_4612 0.68 -0.2 5,337,551 - Pf6N2E2_4612 0.68 -1.2 5,337,559 + Pf6N2E2_4612 0.69 +0.8 5,337,559 + Pf6N2E2_4612 0.69 -1.4 5,337,740 - Pf6N2E2_4612 0.88 -0.8 5,337,740 - Pf6N2E2_4612 0.88 -1.7 5,337,792 - -1.2 5,337,800 + -2.2 5,337,803 - -3.3
Or see this region's nucleotide sequence