Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4393 and Pf6N2E2_4394 are separated by 128 nucleotides Pf6N2E2_4394 and Pf6N2E2_4395 are separated by 182 nucleotides Pf6N2E2_4395 and Pf6N2E2_4396 are separated by 136 nucleotides
Pf6N2E2_4393 - Alanine racemase (EC 5.1.1.1), at 5,121,254 to 5,122,327
_4393
Pf6N2E2_4394 - Putrescine utilization regulator, at 5,122,456 to 5,123,004
_4394
Pf6N2E2_4395 - Cytochrome c5, at 5,123,187 to 5,123,600
_4395
Pf6N2E2_4396 - Acetyl-CoA hydrolase, at 5,123,737 to 5,125,659
_4396
Position (kb)
5122
5123
5124 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5122.331 kb on - strand at 5122.331 kb on - strand at 5122.386 kb on + strand at 5122.386 kb on + strand at 5122.387 kb on - strand at 5122.387 kb on - strand at 5122.390 kb on + strand at 5122.390 kb on + strand at 5122.390 kb on + strand at 5122.391 kb on - strand at 5122.549 kb on - strand, within Pf6N2E2_4394 at 5122.549 kb on - strand, within Pf6N2E2_4394 at 5122.681 kb on + strand, within Pf6N2E2_4394 at 5122.682 kb on - strand, within Pf6N2E2_4394 at 5122.683 kb on + strand, within Pf6N2E2_4394 at 5122.683 kb on + strand, within Pf6N2E2_4394 at 5122.683 kb on + strand, within Pf6N2E2_4394 at 5122.783 kb on + strand, within Pf6N2E2_4394 at 5122.784 kb on - strand, within Pf6N2E2_4394 at 5122.912 kb on + strand, within Pf6N2E2_4394 at 5122.912 kb on + strand, within Pf6N2E2_4394 at 5122.912 kb on + strand, within Pf6N2E2_4394 at 5122.913 kb on - strand, within Pf6N2E2_4394 at 5122.913 kb on - strand, within Pf6N2E2_4394 at 5122.913 kb on - strand, within Pf6N2E2_4394 at 5122.915 kb on + strand, within Pf6N2E2_4394 at 5122.915 kb on + strand, within Pf6N2E2_4394 at 5122.915 kb on + strand, within Pf6N2E2_4394 at 5122.916 kb on - strand, within Pf6N2E2_4394 at 5122.916 kb on - strand, within Pf6N2E2_4394 at 5122.916 kb on - strand, within Pf6N2E2_4394 at 5122.948 kb on - strand, within Pf6N2E2_4394 at 5122.974 kb on + strand at 5122.975 kb on - strand at 5123.111 kb on + strand at 5123.113 kb on + strand at 5123.113 kb on + strand at 5123.113 kb on + strand at 5123.152 kb on + strand at 5123.170 kb on + strand at 5123.171 kb on - strand at 5123.182 kb on + strand at 5123.182 kb on + strand at 5123.182 kb on + strand at 5123.182 kb on + strand at 5123.182 kb on + strand at 5123.182 kb on + strand at 5123.182 kb on + strand at 5123.183 kb on - strand at 5123.183 kb on - strand at 5123.183 kb on - strand at 5123.186 kb on + strand at 5123.186 kb on + strand at 5123.186 kb on + strand at 5123.216 kb on - strand at 5123.216 kb on - strand at 5123.225 kb on - strand at 5123.225 kb on - strand at 5123.598 kb on + strand at 5123.598 kb on + strand at 5123.598 kb on + strand at 5123.601 kb on + strand at 5123.602 kb on - strand at 5123.631 kb on + strand at 5123.632 kb on - strand at 5123.799 kb on + strand at 5123.799 kb on + strand at 5123.800 kb on - strand at 5123.800 kb on - strand at 5123.800 kb on - strand at 5123.899 kb on + strand at 5123.899 kb on + strand at 5123.899 kb on + strand at 5123.899 kb on + strand at 5123.899 kb on + strand at 5123.899 kb on + strand at 5123.899 kb on + strand at 5123.899 kb on + strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.900 kb on - strand at 5123.901 kb on + strand at 5124.004 kb on + strand, within Pf6N2E2_4396
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 5,122,331 - +0.2 5,122,331 - +0.3 5,122,386 + +0.4 5,122,386 + -0.1 5,122,387 - -1.1 5,122,387 - -0.0 5,122,390 + +0.3 5,122,390 + +0.4 5,122,390 + +0.2 5,122,391 - +0.6 5,122,549 - Pf6N2E2_4394 0.17 -0.9 5,122,549 - Pf6N2E2_4394 0.17 -1.4 5,122,681 + Pf6N2E2_4394 0.41 +0.8 5,122,682 - Pf6N2E2_4394 0.41 +0.2 5,122,683 + Pf6N2E2_4394 0.41 -0.7 5,122,683 + Pf6N2E2_4394 0.41 +0.1 5,122,683 + Pf6N2E2_4394 0.41 -0.4 5,122,783 + Pf6N2E2_4394 0.60 -0.8 5,122,784 - Pf6N2E2_4394 0.60 -0.7 5,122,912 + Pf6N2E2_4394 0.83 -0.7 5,122,912 + Pf6N2E2_4394 0.83 -1.2 5,122,912 + Pf6N2E2_4394 0.83 -0.4 5,122,913 - Pf6N2E2_4394 0.83 -0.4 5,122,913 - Pf6N2E2_4394 0.83 -0.3 5,122,913 - Pf6N2E2_4394 0.83 -1.2 5,122,915 + Pf6N2E2_4394 0.84 -0.0 5,122,915 + Pf6N2E2_4394 0.84 -0.2 5,122,915 + Pf6N2E2_4394 0.84 -1.2 5,122,916 - Pf6N2E2_4394 0.84 -0.4 5,122,916 - Pf6N2E2_4394 0.84 -0.4 5,122,916 - Pf6N2E2_4394 0.84 -1.0 5,122,948 - Pf6N2E2_4394 0.90 -0.7 5,122,974 + -0.2 5,122,975 - +0.8 5,123,111 + -1.0 5,123,113 + -1.0 5,123,113 + +1.1 5,123,113 + +1.0 5,123,152 + +0.6 5,123,170 + +0.5 5,123,171 - +0.1 5,123,182 + -0.5 5,123,182 + -2.9 5,123,182 + +0.1 5,123,182 + -0.2 5,123,182 + +1.0 5,123,182 + +0.6 5,123,182 + +1.0 5,123,183 - -0.0 5,123,183 - -0.7 5,123,183 - +0.1 5,123,186 + +0.6 5,123,186 + +0.7 5,123,186 + +0.6 5,123,216 - -0.6 5,123,216 - +0.2 5,123,225 - +1.6 5,123,225 - -0.0 5,123,598 + +0.6 5,123,598 + +1.0 5,123,598 + +0.2 5,123,601 + +0.7 5,123,602 - -0.5 5,123,631 + +1.6 5,123,632 - -0.9 5,123,799 + +0.4 5,123,799 + -0.0 5,123,800 - -0.1 5,123,800 - +1.0 5,123,800 - -0.6 5,123,899 + -2.3 5,123,899 + +0.5 5,123,899 + +0.1 5,123,899 + +1.0 5,123,899 + +0.1 5,123,899 + -0.0 5,123,899 + +0.6 5,123,899 + -0.7 5,123,900 - +1.0 5,123,900 - -2.5 5,123,900 - +1.2 5,123,900 - +1.3 5,123,900 - -0.1 5,123,900 - +0.3 5,123,900 - +0.2 5,123,900 - -0.6 5,123,900 - +0.5 5,123,900 - -0.8 5,123,900 - +0.2 5,123,900 - +0.6 5,123,900 - -0.1 5,123,900 - +0.2 5,123,900 - +0.8 5,123,900 - +0.0 5,123,900 - -0.2 5,123,900 - +0.5 5,123,900 - +0.5 5,123,900 - +0.5 5,123,900 - -0.7 5,123,900 - +0.4 5,123,900 - +0.7 5,123,900 - +0.1 5,123,901 + -0.4 5,124,004 + Pf6N2E2_4396 0.14 -0.1
Or see this region's nucleotide sequence