Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4391 and Pf6N2E2_4392 overlap by 29 nucleotides Pf6N2E2_4392 and Pf6N2E2_4393 are separated by 97 nucleotides
Pf6N2E2_4391 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 5,119,527 to 5,120,831
_4391
Pf6N2E2_4392 - Translation initiation inhibitor, at 5,120,803 to 5,121,156
_4392
Pf6N2E2_4393 - Alanine racemase (EC 5.1.1.1), at 5,121,254 to 5,122,327
_4393
Position (kb)
5120
5121
5122 Strain fitness (log2 ratio)
-1
0
1
2 at 5119.867 kb on - strand, within Pf6N2E2_4391 at 5119.914 kb on + strand, within Pf6N2E2_4391 at 5119.914 kb on + strand, within Pf6N2E2_4391 at 5120.023 kb on + strand, within Pf6N2E2_4391 at 5120.135 kb on + strand, within Pf6N2E2_4391 at 5120.292 kb on + strand, within Pf6N2E2_4391 at 5120.352 kb on + strand, within Pf6N2E2_4391 at 5120.352 kb on + strand, within Pf6N2E2_4391 at 5120.418 kb on + strand, within Pf6N2E2_4391 at 5120.418 kb on + strand, within Pf6N2E2_4391 at 5120.418 kb on + strand, within Pf6N2E2_4391 at 5120.418 kb on + strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.419 kb on - strand, within Pf6N2E2_4391 at 5120.420 kb on + strand, within Pf6N2E2_4391 at 5120.420 kb on + strand, within Pf6N2E2_4391 at 5120.762 kb on + strand at 5120.762 kb on + strand at 5120.762 kb on + strand at 5120.763 kb on - strand at 5120.869 kb on + strand, within Pf6N2E2_4392 at 5121.001 kb on + strand, within Pf6N2E2_4392 at 5121.154 kb on + strand at 5121.158 kb on + strand at 5121.158 kb on + strand at 5121.158 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 5,119,867 - Pf6N2E2_4391 0.26 +1.9 5,119,914 + Pf6N2E2_4391 0.30 +0.3 5,119,914 + Pf6N2E2_4391 0.30 +0.4 5,120,023 + Pf6N2E2_4391 0.38 -1.1 5,120,135 + Pf6N2E2_4391 0.47 -1.2 5,120,292 + Pf6N2E2_4391 0.59 -1.0 5,120,352 + Pf6N2E2_4391 0.63 +1.5 5,120,352 + Pf6N2E2_4391 0.63 +0.8 5,120,418 + Pf6N2E2_4391 0.68 +0.8 5,120,418 + Pf6N2E2_4391 0.68 +0.8 5,120,418 + Pf6N2E2_4391 0.68 -1.5 5,120,418 + Pf6N2E2_4391 0.68 +0.6 5,120,419 - Pf6N2E2_4391 0.68 +1.3 5,120,419 - Pf6N2E2_4391 0.68 +0.5 5,120,419 - Pf6N2E2_4391 0.68 -1.7 5,120,419 - Pf6N2E2_4391 0.68 +2.6 5,120,419 - Pf6N2E2_4391 0.68 +0.5 5,120,420 + Pf6N2E2_4391 0.68 +1.7 5,120,420 + Pf6N2E2_4391 0.68 +0.6 5,120,762 + +0.6 5,120,762 + -0.2 5,120,762 + +0.1 5,120,763 - -0.2 5,120,869 + Pf6N2E2_4392 0.19 -1.2 5,121,001 + Pf6N2E2_4392 0.56 -0.2 5,121,154 + +0.3 5,121,158 + +0.1 5,121,158 + +0.4 5,121,158 + +0.5
Or see this region's nucleotide sequence