Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4362

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4361 and Pf6N2E2_4362 are separated by 60 nucleotidesPf6N2E2_4362 and Pf6N2E2_4363 are separated by 167 nucleotidesPf6N2E2_4363 and Pf6N2E2_4364 are separated by 10 nucleotides Pf6N2E2_4361 - GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 5,090,952 to 5,093,057 _4361 Pf6N2E2_4362 - DNA-directed RNA polymerase omega subunit (EC 2.7.7.6), at 5,093,118 to 5,093,381 _4362 Pf6N2E2_4363 - Guanylate kinase (EC 2.7.4.8), at 5,093,549 to 5,094,169 _4363 Pf6N2E2_4364 - Protein YicC, at 5,094,180 to 5,095,043 _4364 Position (kb) 5093 5094Strain fitness (log2 ratio) -3 -2 -1 0 1at 5093.047 kb on - strandat 5093.099 kb on - strandat 5093.099 kb on - strandat 5093.099 kb on - strandat 5093.114 kb on - strandat 5093.114 kb on - strandat 5093.114 kb on - strandat 5093.114 kb on - strandat 5093.120 kb on - strandat 5093.201 kb on - strand, within Pf6N2E2_4362at 5093.236 kb on - strand, within Pf6N2E2_4362at 5093.239 kb on - strand, within Pf6N2E2_4362at 5093.244 kb on - strand, within Pf6N2E2_4362at 5093.244 kb on - strand, within Pf6N2E2_4362at 5093.244 kb on - strand, within Pf6N2E2_4362at 5093.244 kb on - strand, within Pf6N2E2_4362at 5093.244 kb on - strand, within Pf6N2E2_4362at 5093.385 kb on - strandat 5093.385 kb on - strandat 5093.508 kb on + strandat 5093.508 kb on + strandat 5093.516 kb on + strandat 5093.516 kb on + strandat 5093.516 kb on + strandat 5093.516 kb on + strandat 5093.516 kb on + strandat 5093.517 kb on - strandat 5093.517 kb on - strandat 5093.517 kb on - strandat 5093.517 kb on - strandat 5093.517 kb on - strandat 5093.570 kb on + strandat 5093.571 kb on - strandat 5093.571 kb on - strandat 5093.571 kb on - strandat 5093.571 kb on - strandat 5094.182 kb on - strandat 5094.182 kb on - strandat 5094.282 kb on - strand, within Pf6N2E2_4364at 5094.282 kb on - strand, within Pf6N2E2_4364at 5094.282 kb on - strand, within Pf6N2E2_4364at 5094.282 kb on - strand, within Pf6N2E2_4364

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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5,093,047 - -0.4
5,093,099 - +0.3
5,093,099 - +0.9
5,093,099 - -2.2
5,093,114 - -1.2
5,093,114 - -2.6
5,093,114 - -0.7
5,093,114 - -0.8
5,093,120 - -1.2
5,093,201 - Pf6N2E2_4362 0.31 -2.5
5,093,236 - Pf6N2E2_4362 0.45 -0.1
5,093,239 - Pf6N2E2_4362 0.46 -2.7
5,093,244 - Pf6N2E2_4362 0.48 -1.5
5,093,244 - Pf6N2E2_4362 0.48 -0.4
5,093,244 - Pf6N2E2_4362 0.48 +0.3
5,093,244 - Pf6N2E2_4362 0.48 -1.9
5,093,244 - Pf6N2E2_4362 0.48 -2.2
5,093,385 - -0.7
5,093,385 - -3.1
5,093,508 + -1.6
5,093,508 + -0.7
5,093,516 + +0.4
5,093,516 + -0.4
5,093,516 + -0.5
5,093,516 + +0.2
5,093,516 + +0.2
5,093,517 - -0.3
5,093,517 - -0.7
5,093,517 - +0.4
5,093,517 - +0.7
5,093,517 - +0.4
5,093,570 + +0.8
5,093,571 - +0.4
5,093,571 - +0.7
5,093,571 - +0.7
5,093,571 - +0.1
5,094,182 - -1.3
5,094,182 - +0.7
5,094,282 - Pf6N2E2_4364 0.12 +0.2
5,094,282 - Pf6N2E2_4364 0.12 +0.9
5,094,282 - Pf6N2E2_4364 0.12 +0.2
5,094,282 - Pf6N2E2_4364 0.12 -1.0

Or see this region's nucleotide sequence