Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_422 and Pf6N2E2_423 are separated by 20 nucleotides Pf6N2E2_423 and Pf6N2E2_424 are separated by 129 nucleotides Pf6N2E2_424 and Pf6N2E2_425 are separated by 217 nucleotides Pf6N2E2_425 and Pf6N2E2_426 are separated by 4 nucleotides
Pf6N2E2_422 - Na+ driven multidrug efflux pump, at 490,565 to 491,863
_422
Pf6N2E2_423 - Putative phosphatase YfbT, at 491,884 to 492,588
_423
Pf6N2E2_424 - Decarboxylase family protein, at 492,718 to 494,091
_424
Pf6N2E2_425 - FIG00957304: hypothetical protein, at 494,309 to 494,842
_425
Pf6N2E2_426 - Arsenate reductase (EC 1.20.4.1), at 494,847 to 495,302
_426
Position (kb) 492
493
494
495 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 491.729 kb on + strand, within Pf6N2E2_422 at 491.730 kb on - strand, within Pf6N2E2_422 at 491.786 kb on + strand at 491.787 kb on - strand at 491.817 kb on - strand at 491.819 kb on + strand at 491.820 kb on - strand at 491.861 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.878 kb on - strand at 491.878 kb on - strand at 491.878 kb on - strand at 491.878 kb on - strand at 492.279 kb on + strand, within Pf6N2E2_423 at 492.486 kb on + strand, within Pf6N2E2_423 at 492.526 kb on + strand at 492.526 kb on + strand at 492.527 kb on - strand at 492.527 kb on - strand at 492.527 kb on - strand at 492.584 kb on + strand at 492.584 kb on + strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.660 kb on + strand at 492.714 kb on + strand at 492.715 kb on - strand at 492.746 kb on + strand at 492.746 kb on + strand at 492.747 kb on - strand at 492.747 kb on - strand at 492.747 kb on - strand at 492.747 kb on - strand at 493.143 kb on - strand, within Pf6N2E2_424 at 493.143 kb on - strand, within Pf6N2E2_424 at 493.348 kb on + strand, within Pf6N2E2_424 at 493.348 kb on + strand, within Pf6N2E2_424 at 493.349 kb on - strand, within Pf6N2E2_424 at 493.470 kb on + strand, within Pf6N2E2_424 at 493.471 kb on - strand, within Pf6N2E2_424 at 493.545 kb on + strand, within Pf6N2E2_424 at 493.546 kb on - strand, within Pf6N2E2_424 at 493.589 kb on + strand, within Pf6N2E2_424 at 493.590 kb on - strand, within Pf6N2E2_424 at 493.590 kb on - strand, within Pf6N2E2_424 at 493.590 kb on - strand, within Pf6N2E2_424 at 493.595 kb on + strand, within Pf6N2E2_424 at 493.595 kb on + strand, within Pf6N2E2_424 at 493.595 kb on + strand, within Pf6N2E2_424 at 493.694 kb on + strand, within Pf6N2E2_424 at 493.695 kb on - strand, within Pf6N2E2_424 at 493.695 kb on - strand, within Pf6N2E2_424 at 493.901 kb on + strand, within Pf6N2E2_424 at 493.902 kb on - strand, within Pf6N2E2_424 at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.982 kb on + strand at 493.983 kb on - strand at 493.983 kb on - strand at 493.986 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.074 kb on + strand at 494.075 kb on - strand at 494.075 kb on - strand at 494.075 kb on - strand at 494.241 kb on + strand at 494.241 kb on + strand at 494.241 kb on + strand at 494.242 kb on - strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.245 kb on + strand at 494.246 kb on - strand at 494.246 kb on - strand at 494.246 kb on - strand at 494.246 kb on - strand at 494.246 kb on - strand at 494.289 kb on + strand at 494.289 kb on + strand at 494.290 kb on - strand at 494.290 kb on - strand at 494.290 kb on - strand at 494.342 kb on + strand at 494.343 kb on - strand at 494.343 kb on - strand at 494.343 kb on - strand at 494.519 kb on + strand, within Pf6N2E2_425 at 494.520 kb on - strand, within Pf6N2E2_425 at 494.555 kb on + strand, within Pf6N2E2_425 at 494.664 kb on - strand, within Pf6N2E2_425 at 494.664 kb on - strand, within Pf6N2E2_425 at 494.828 kb on + strand at 495.046 kb on + strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 491,729 + Pf6N2E2_422 0.90 +1.8 491,730 - Pf6N2E2_422 0.90 +1.5 491,786 + +1.5 491,787 - -1.0 491,817 - +0.8 491,819 + -0.4 491,820 - +0.5 491,861 + -0.2 491,877 + +1.1 491,877 + +0.3 491,877 + +0.2 491,877 + -0.2 491,878 - +0.8 491,878 - +0.3 491,878 - -0.2 491,878 - +0.8 492,279 + Pf6N2E2_423 0.56 -0.2 492,486 + Pf6N2E2_423 0.85 +0.3 492,526 + -0.5 492,526 + +0.3 492,527 - -0.6 492,527 - +0.0 492,527 - -1.5 492,584 + -0.0 492,584 + -0.1 492,585 - -0.1 492,585 - -0.5 492,585 - +1.0 492,585 - -1.2 492,660 + -0.1 492,714 + +1.2 492,715 - +0.1 492,746 + -2.1 492,746 + -0.2 492,747 - -0.6 492,747 - -1.8 492,747 - -0.1 492,747 - -0.2 493,143 - Pf6N2E2_424 0.31 -1.5 493,143 - Pf6N2E2_424 0.31 -1.6 493,348 + Pf6N2E2_424 0.46 -0.4 493,348 + Pf6N2E2_424 0.46 -0.1 493,349 - Pf6N2E2_424 0.46 -0.7 493,470 + Pf6N2E2_424 0.55 -0.8 493,471 - Pf6N2E2_424 0.55 -1.1 493,545 + Pf6N2E2_424 0.60 -3.1 493,546 - Pf6N2E2_424 0.60 +0.7 493,589 + Pf6N2E2_424 0.63 -0.9 493,590 - Pf6N2E2_424 0.63 -2.1 493,590 - Pf6N2E2_424 0.63 -0.1 493,590 - Pf6N2E2_424 0.63 -3.1 493,595 + Pf6N2E2_424 0.64 -0.4 493,595 + Pf6N2E2_424 0.64 +1.1 493,595 + Pf6N2E2_424 0.64 +0.0 493,694 + Pf6N2E2_424 0.71 +0.7 493,695 - Pf6N2E2_424 0.71 +0.2 493,695 - Pf6N2E2_424 0.71 -0.4 493,901 + Pf6N2E2_424 0.86 +1.1 493,902 - Pf6N2E2_424 0.86 +0.3 493,982 + -0.3 493,982 + +0.2 493,982 + -1.7 493,982 + +0.2 493,982 + +2.1 493,982 + -1.4 493,982 + -0.5 493,982 + -1.4 493,982 + -0.7 493,983 - -1.3 493,983 - +0.1 493,986 + -0.3 494,074 + -1.4 494,074 + -0.3 494,074 + -0.7 494,074 + -2.2 494,074 + +0.3 494,074 + -1.7 494,075 - -0.9 494,075 - -0.6 494,075 - -0.4 494,241 + -1.6 494,241 + -0.4 494,241 + -0.6 494,242 - +0.2 494,245 + +0.4 494,245 + -1.5 494,245 + +0.0 494,245 + +1.3 494,245 + +1.1 494,245 + +0.2 494,246 - -0.1 494,246 - +0.5 494,246 - +0.5 494,246 - +0.2 494,246 - +0.3 494,289 + +1.1 494,289 + +1.3 494,290 - -0.3 494,290 - +0.1 494,290 - +0.5 494,342 + +0.3 494,343 - +0.2 494,343 - +0.7 494,343 - +0.5 494,519 + Pf6N2E2_425 0.39 +1.5 494,520 - Pf6N2E2_425 0.40 -0.1 494,555 + Pf6N2E2_425 0.46 -0.6 494,664 - Pf6N2E2_425 0.66 -0.5 494,664 - Pf6N2E2_425 0.66 -0.8 494,828 + -0.8 495,046 + Pf6N2E2_426 0.44 -0.3 495,047 - Pf6N2E2_426 0.44 -0.4 495,047 - Pf6N2E2_426 0.44 -1.3 495,047 - Pf6N2E2_426 0.44 +0.7
Or see this region's nucleotide sequence