Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4187

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4186 and Pf6N2E2_4187 are separated by 163 nucleotidesPf6N2E2_4187 and Pf6N2E2_4188 are separated by 43 nucleotidesPf6N2E2_4188 and Pf6N2E2_4189 are separated by 120 nucleotides Pf6N2E2_4186 - Glycine oxidase ThiO (EC 1.4.3.19), at 4,871,092 to 4,872,138 _4186 Pf6N2E2_4187 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 4,872,302 to 4,873,189 _4187 Pf6N2E2_4188 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 4,873,233 to 4,873,751 _4188 Pf6N2E2_4189 - Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14), at 4,873,872 to 4,874,825 _4189 Position (kb) 4872 4873 4874Strain fitness (log2 ratio) -2 -1 0 1 2at 4871.305 kb on + strand, within Pf6N2E2_4186at 4871.305 kb on + strand, within Pf6N2E2_4186at 4871.305 kb on + strand, within Pf6N2E2_4186at 4871.305 kb on + strand, within Pf6N2E2_4186at 4871.305 kb on + strand, within Pf6N2E2_4186at 4871.306 kb on - strand, within Pf6N2E2_4186at 4871.306 kb on - strand, within Pf6N2E2_4186at 4871.306 kb on - strand, within Pf6N2E2_4186at 4871.306 kb on - strand, within Pf6N2E2_4186at 4871.306 kb on - strand, within Pf6N2E2_4186at 4871.306 kb on - strand, within Pf6N2E2_4186at 4871.403 kb on - strand, within Pf6N2E2_4186at 4871.498 kb on + strand, within Pf6N2E2_4186at 4871.499 kb on - strand, within Pf6N2E2_4186at 4871.527 kb on + strand, within Pf6N2E2_4186at 4871.587 kb on + strand, within Pf6N2E2_4186at 4871.587 kb on + strand, within Pf6N2E2_4186at 4871.587 kb on + strand, within Pf6N2E2_4186at 4871.656 kb on - strand, within Pf6N2E2_4186at 4871.656 kb on - strand, within Pf6N2E2_4186at 4871.735 kb on - strand, within Pf6N2E2_4186at 4871.765 kb on + strand, within Pf6N2E2_4186at 4871.862 kb on - strand, within Pf6N2E2_4186at 4872.072 kb on - strandat 4872.088 kb on + strandat 4872.088 kb on + strandat 4872.089 kb on - strandat 4872.238 kb on + strandat 4872.307 kb on - strandat 4872.360 kb on + strandat 4872.361 kb on - strandat 4872.384 kb on + strandat 4872.438 kb on + strand, within Pf6N2E2_4187at 4872.438 kb on + strand, within Pf6N2E2_4187at 4872.438 kb on + strand, within Pf6N2E2_4187at 4872.438 kb on + strandat 4872.438 kb on + strand, within Pf6N2E2_4187at 4872.438 kb on + strand, within Pf6N2E2_4187at 4872.439 kb on - strand, within Pf6N2E2_4187at 4872.439 kb on - strand, within Pf6N2E2_4187at 4872.439 kb on - strand, within Pf6N2E2_4187at 4872.439 kb on - strand, within Pf6N2E2_4187at 4872.439 kb on - strand, within Pf6N2E2_4187at 4872.439 kb on - strand, within Pf6N2E2_4187at 4872.798 kb on + strand, within Pf6N2E2_4187at 4872.799 kb on - strand, within Pf6N2E2_4187at 4872.799 kb on - strand, within Pf6N2E2_4187at 4873.206 kb on + strandat 4873.207 kb on - strandat 4873.207 kb on - strandat 4873.315 kb on + strand, within Pf6N2E2_4188at 4873.437 kb on - strand, within Pf6N2E2_4188at 4873.609 kb on + strand, within Pf6N2E2_4188at 4873.610 kb on - strand, within Pf6N2E2_4188at 4873.744 kb on + strandat 4873.744 kb on + strandat 4873.745 kb on - strandat 4873.753 kb on + strandat 4873.754 kb on - strandat 4873.754 kb on - strandat 4873.754 kb on - strandat 4873.791 kb on - strandat 4873.791 kb on - strandat 4873.791 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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4,871,305 + Pf6N2E2_4186 0.20 +0.4
4,871,305 + Pf6N2E2_4186 0.20 +0.2
4,871,305 + Pf6N2E2_4186 0.20 +1.5
4,871,305 + Pf6N2E2_4186 0.20 -1.0
4,871,305 + Pf6N2E2_4186 0.20 +0.4
4,871,306 - Pf6N2E2_4186 0.20 -0.5
4,871,306 - Pf6N2E2_4186 0.20 +0.5
4,871,306 - Pf6N2E2_4186 0.20 +0.4
4,871,306 - Pf6N2E2_4186 0.20 -0.1
4,871,306 - Pf6N2E2_4186 0.20 -0.1
4,871,306 - Pf6N2E2_4186 0.20 +0.9
4,871,403 - Pf6N2E2_4186 0.30 +0.0
4,871,498 + Pf6N2E2_4186 0.39 +0.1
4,871,499 - Pf6N2E2_4186 0.39 +1.8
4,871,527 + Pf6N2E2_4186 0.42 +0.5
4,871,587 + Pf6N2E2_4186 0.47 -1.0
4,871,587 + Pf6N2E2_4186 0.47 +0.3
4,871,587 + Pf6N2E2_4186 0.47 -0.4
4,871,656 - Pf6N2E2_4186 0.54 +0.3
4,871,656 - Pf6N2E2_4186 0.54 -0.6
4,871,735 - Pf6N2E2_4186 0.61 +0.4
4,871,765 + Pf6N2E2_4186 0.64 +0.9
4,871,862 - Pf6N2E2_4186 0.74 -2.3
4,872,072 - -0.2
4,872,088 + -0.0
4,872,088 + +0.6
4,872,089 - +1.0
4,872,238 + -0.4
4,872,307 - -2.0
4,872,360 + -0.7
4,872,361 - -0.3
4,872,384 + -1.5
4,872,438 + Pf6N2E2_4187 0.15 +0.0
4,872,438 + Pf6N2E2_4187 0.15 -2.2
4,872,438 + Pf6N2E2_4187 0.15 +0.6
4,872,438 + +0.6
4,872,438 + Pf6N2E2_4187 0.15 -0.5
4,872,438 + Pf6N2E2_4187 0.15 -1.4
4,872,439 - Pf6N2E2_4187 0.15 -1.1
4,872,439 - Pf6N2E2_4187 0.15 -2.2
4,872,439 - Pf6N2E2_4187 0.15 -1.7
4,872,439 - Pf6N2E2_4187 0.15 -0.1
4,872,439 - Pf6N2E2_4187 0.15 -1.0
4,872,439 - Pf6N2E2_4187 0.15 -1.7
4,872,798 + Pf6N2E2_4187 0.56 -1.7
4,872,799 - Pf6N2E2_4187 0.56 -0.8
4,872,799 - Pf6N2E2_4187 0.56 -2.4
4,873,206 + -1.0
4,873,207 - -1.2
4,873,207 - -0.0
4,873,315 + Pf6N2E2_4188 0.16 +0.5
4,873,437 - Pf6N2E2_4188 0.39 +1.1
4,873,609 + Pf6N2E2_4188 0.72 +0.6
4,873,610 - Pf6N2E2_4188 0.73 +0.7
4,873,744 + +0.9
4,873,744 + -0.4
4,873,745 - +1.8
4,873,753 + -0.5
4,873,754 - +0.7
4,873,754 - -0.6
4,873,754 - -0.9
4,873,791 - -0.7
4,873,791 - +0.5
4,873,791 - -0.5

Or see this region's nucleotide sequence