Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4186 and Pf6N2E2_4187 are separated by 163 nucleotides Pf6N2E2_4187 and Pf6N2E2_4188 are separated by 43 nucleotides Pf6N2E2_4188 and Pf6N2E2_4189 are separated by 120 nucleotides
Pf6N2E2_4186 - Glycine oxidase ThiO (EC 1.4.3.19), at 4,871,092 to 4,872,138
_4186
Pf6N2E2_4187 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 4,872,302 to 4,873,189
_4187
Pf6N2E2_4188 - DNA-3-methyladenine glycosylase (EC 3.2.2.20), at 4,873,233 to 4,873,751
_4188
Pf6N2E2_4189 - Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14), at 4,873,872 to 4,874,825
_4189
Position (kb)
4872
4873
4874 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.403 kb on - strand, within Pf6N2E2_4186 at 4871.498 kb on + strand, within Pf6N2E2_4186 at 4871.499 kb on - strand, within Pf6N2E2_4186 at 4871.527 kb on + strand, within Pf6N2E2_4186 at 4871.587 kb on + strand, within Pf6N2E2_4186 at 4871.587 kb on + strand, within Pf6N2E2_4186 at 4871.587 kb on + strand, within Pf6N2E2_4186 at 4871.656 kb on - strand, within Pf6N2E2_4186 at 4871.656 kb on - strand, within Pf6N2E2_4186 at 4871.735 kb on - strand, within Pf6N2E2_4186 at 4871.765 kb on + strand, within Pf6N2E2_4186 at 4871.862 kb on - strand, within Pf6N2E2_4186 at 4872.072 kb on - strand at 4872.088 kb on + strand at 4872.088 kb on + strand at 4872.089 kb on - strand at 4872.238 kb on + strand at 4872.307 kb on - strand at 4872.360 kb on + strand at 4872.361 kb on - strand at 4872.384 kb on + strand at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.438 kb on + strand at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.798 kb on + strand, within Pf6N2E2_4187 at 4872.799 kb on - strand, within Pf6N2E2_4187 at 4872.799 kb on - strand, within Pf6N2E2_4187 at 4873.206 kb on + strand at 4873.207 kb on - strand at 4873.207 kb on - strand at 4873.315 kb on + strand, within Pf6N2E2_4188 at 4873.437 kb on - strand, within Pf6N2E2_4188 at 4873.609 kb on + strand, within Pf6N2E2_4188 at 4873.610 kb on - strand, within Pf6N2E2_4188 at 4873.744 kb on + strand at 4873.744 kb on + strand at 4873.745 kb on - strand at 4873.753 kb on + strand at 4873.754 kb on - strand at 4873.754 kb on - strand at 4873.754 kb on - strand at 4873.791 kb on - strand at 4873.791 kb on - strand at 4873.791 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 4,871,305 + Pf6N2E2_4186 0.20 +0.4 4,871,305 + Pf6N2E2_4186 0.20 +0.2 4,871,305 + Pf6N2E2_4186 0.20 +1.5 4,871,305 + Pf6N2E2_4186 0.20 -1.0 4,871,305 + Pf6N2E2_4186 0.20 +0.4 4,871,306 - Pf6N2E2_4186 0.20 -0.5 4,871,306 - Pf6N2E2_4186 0.20 +0.5 4,871,306 - Pf6N2E2_4186 0.20 +0.4 4,871,306 - Pf6N2E2_4186 0.20 -0.1 4,871,306 - Pf6N2E2_4186 0.20 -0.1 4,871,306 - Pf6N2E2_4186 0.20 +0.9 4,871,403 - Pf6N2E2_4186 0.30 +0.0 4,871,498 + Pf6N2E2_4186 0.39 +0.1 4,871,499 - Pf6N2E2_4186 0.39 +1.8 4,871,527 + Pf6N2E2_4186 0.42 +0.5 4,871,587 + Pf6N2E2_4186 0.47 -1.0 4,871,587 + Pf6N2E2_4186 0.47 +0.3 4,871,587 + Pf6N2E2_4186 0.47 -0.4 4,871,656 - Pf6N2E2_4186 0.54 +0.3 4,871,656 - Pf6N2E2_4186 0.54 -0.6 4,871,735 - Pf6N2E2_4186 0.61 +0.4 4,871,765 + Pf6N2E2_4186 0.64 +0.9 4,871,862 - Pf6N2E2_4186 0.74 -2.3 4,872,072 - -0.2 4,872,088 + -0.0 4,872,088 + +0.6 4,872,089 - +1.0 4,872,238 + -0.4 4,872,307 - -2.0 4,872,360 + -0.7 4,872,361 - -0.3 4,872,384 + -1.5 4,872,438 + Pf6N2E2_4187 0.15 +0.0 4,872,438 + Pf6N2E2_4187 0.15 -2.2 4,872,438 + Pf6N2E2_4187 0.15 +0.6 4,872,438 + +0.6 4,872,438 + Pf6N2E2_4187 0.15 -0.5 4,872,438 + Pf6N2E2_4187 0.15 -1.4 4,872,439 - Pf6N2E2_4187 0.15 -1.1 4,872,439 - Pf6N2E2_4187 0.15 -2.2 4,872,439 - Pf6N2E2_4187 0.15 -1.7 4,872,439 - Pf6N2E2_4187 0.15 -0.1 4,872,439 - Pf6N2E2_4187 0.15 -1.0 4,872,439 - Pf6N2E2_4187 0.15 -1.7 4,872,798 + Pf6N2E2_4187 0.56 -1.7 4,872,799 - Pf6N2E2_4187 0.56 -0.8 4,872,799 - Pf6N2E2_4187 0.56 -2.4 4,873,206 + -1.0 4,873,207 - -1.2 4,873,207 - -0.0 4,873,315 + Pf6N2E2_4188 0.16 +0.5 4,873,437 - Pf6N2E2_4188 0.39 +1.1 4,873,609 + Pf6N2E2_4188 0.72 +0.6 4,873,610 - Pf6N2E2_4188 0.73 +0.7 4,873,744 + +0.9 4,873,744 + -0.4 4,873,745 - +1.8 4,873,753 + -0.5 4,873,754 - +0.7 4,873,754 - -0.6 4,873,754 - -0.9 4,873,791 - -0.7 4,873,791 - +0.5 4,873,791 - -0.5
Or see this region's nucleotide sequence