Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_388 and Pf6N2E2_389 are separated by 216 nucleotides Pf6N2E2_389 and Pf6N2E2_390 are separated by 58 nucleotides Pf6N2E2_390 and Pf6N2E2_391 are separated by 48 nucleotides
Pf6N2E2_388 - Transporter, MFS superfamily, at 455,437 to 456,780
_388
Pf6N2E2_389 - Choline dehydrogenase (EC 1.1.99.1), at 456,997 to 458,562
_389
Pf6N2E2_390 - hypothetical protein, at 458,621 to 459,394
_390
Pf6N2E2_391 - hypothetical protein, at 459,443 to 460,921
_391
Position (kb) 456
457
458
459 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 456.008 kb on + strand, within Pf6N2E2_388 at 456.008 kb on + strand, within Pf6N2E2_388 at 456.009 kb on - strand, within Pf6N2E2_388 at 456.028 kb on + strand, within Pf6N2E2_388 at 456.031 kb on - strand, within Pf6N2E2_388 at 456.149 kb on + strand, within Pf6N2E2_388 at 456.167 kb on + strand, within Pf6N2E2_388 at 456.167 kb on + strand, within Pf6N2E2_388 at 456.202 kb on - strand, within Pf6N2E2_388 at 456.209 kb on + strand, within Pf6N2E2_388 at 456.209 kb on + strand, within Pf6N2E2_388 at 456.254 kb on - strand, within Pf6N2E2_388 at 456.280 kb on + strand, within Pf6N2E2_388 at 456.280 kb on + strand, within Pf6N2E2_388 at 456.280 kb on + strand, within Pf6N2E2_388 at 456.530 kb on + strand, within Pf6N2E2_388 at 456.531 kb on - strand, within Pf6N2E2_388 at 456.531 kb on - strand, within Pf6N2E2_388 at 456.532 kb on + strand, within Pf6N2E2_388 at 456.532 kb on + strand, within Pf6N2E2_388 at 456.532 kb on + strand, within Pf6N2E2_388 at 456.532 kb on + strand, within Pf6N2E2_388 at 456.533 kb on - strand, within Pf6N2E2_388 at 456.533 kb on - strand, within Pf6N2E2_388 at 456.533 kb on - strand, within Pf6N2E2_388 at 456.655 kb on + strand at 456.656 kb on - strand at 456.697 kb on + strand at 456.698 kb on - strand at 456.736 kb on + strand at 456.736 kb on + strand at 456.765 kb on + strand at 456.766 kb on - strand at 456.778 kb on + strand at 456.778 kb on + strand at 456.778 kb on + strand at 456.779 kb on - strand at 456.875 kb on + strand at 456.875 kb on + strand at 456.875 kb on + strand at 456.875 kb on + strand at 456.875 kb on + strand at 456.881 kb on + strand at 456.881 kb on + strand at 456.938 kb on - strand at 456.938 kb on - strand at 456.966 kb on - strand at 456.966 kb on - strand at 456.998 kb on + strand at 457.143 kb on - strand at 457.143 kb on - strand at 457.214 kb on + strand, within Pf6N2E2_389 at 457.583 kb on + strand, within Pf6N2E2_389 at 457.583 kb on + strand, within Pf6N2E2_389 at 457.583 kb on + strand, within Pf6N2E2_389 at 457.584 kb on - strand, within Pf6N2E2_389 at 457.584 kb on - strand, within Pf6N2E2_389 at 457.632 kb on - strand, within Pf6N2E2_389 at 457.641 kb on + strand, within Pf6N2E2_389 at 457.808 kb on + strand, within Pf6N2E2_389 at 457.937 kb on + strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.947 kb on - strand, within Pf6N2E2_389 at 457.978 kb on + strand, within Pf6N2E2_389 at 457.979 kb on - strand, within Pf6N2E2_389 at 457.979 kb on - strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.538 kb on - strand at 458.538 kb on - strand at 458.794 kb on - strand, within Pf6N2E2_390 at 458.794 kb on - strand, within Pf6N2E2_390 at 458.794 kb on - strand, within Pf6N2E2_390 at 458.943 kb on + strand, within Pf6N2E2_390 at 458.944 kb on - strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.055 kb on - strand, within Pf6N2E2_390 at 459.288 kb on - strand, within Pf6N2E2_390 at 459.288 kb on - strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.459 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 456,008 + Pf6N2E2_388 0.42 -0.2 456,008 + Pf6N2E2_388 0.42 -0.0 456,009 - Pf6N2E2_388 0.43 -0.2 456,028 + Pf6N2E2_388 0.44 +0.6 456,031 - Pf6N2E2_388 0.44 +1.1 456,149 + Pf6N2E2_388 0.53 +0.2 456,167 + Pf6N2E2_388 0.54 +0.1 456,167 + Pf6N2E2_388 0.54 -1.0 456,202 - Pf6N2E2_388 0.57 +0.9 456,209 + Pf6N2E2_388 0.57 +0.6 456,209 + Pf6N2E2_388 0.57 +0.7 456,254 - Pf6N2E2_388 0.61 -0.6 456,280 + Pf6N2E2_388 0.63 -0.1 456,280 + Pf6N2E2_388 0.63 -1.1 456,280 + Pf6N2E2_388 0.63 +0.2 456,530 + Pf6N2E2_388 0.81 +2.3 456,531 - Pf6N2E2_388 0.81 +1.3 456,531 - Pf6N2E2_388 0.81 +0.3 456,532 + Pf6N2E2_388 0.81 -0.6 456,532 + Pf6N2E2_388 0.81 +1.1 456,532 + Pf6N2E2_388 0.81 -0.3 456,532 + Pf6N2E2_388 0.81 -0.2 456,533 - Pf6N2E2_388 0.82 +0.4 456,533 - Pf6N2E2_388 0.82 -0.2 456,533 - Pf6N2E2_388 0.82 -0.6 456,655 + -0.3 456,656 - -0.2 456,697 + +1.3 456,698 - -0.0 456,736 + +0.6 456,736 + -0.5 456,765 + -1.0 456,766 - +0.2 456,778 + -0.2 456,778 + -0.3 456,778 + +0.6 456,779 - +1.1 456,875 + +0.1 456,875 + +0.0 456,875 + +0.9 456,875 + -0.8 456,875 + +0.8 456,881 + +0.0 456,881 + -0.8 456,938 - +1.8 456,938 - -0.1 456,966 - +0.8 456,966 - -0.7 456,998 + -0.3 457,143 - -0.1 457,143 - -0.3 457,214 + Pf6N2E2_389 0.14 -0.5 457,583 + Pf6N2E2_389 0.37 -0.8 457,583 + Pf6N2E2_389 0.37 -0.7 457,583 + Pf6N2E2_389 0.37 +1.0 457,584 - Pf6N2E2_389 0.37 +0.0 457,584 - Pf6N2E2_389 0.37 -1.1 457,632 - Pf6N2E2_389 0.41 +0.5 457,641 + Pf6N2E2_389 0.41 -0.2 457,808 + Pf6N2E2_389 0.52 -0.8 457,937 + Pf6N2E2_389 0.60 +2.1 457,938 - Pf6N2E2_389 0.60 +0.2 457,938 - Pf6N2E2_389 0.60 -1.1 457,938 - Pf6N2E2_389 0.60 +0.2 457,938 - Pf6N2E2_389 0.60 +0.2 457,947 - Pf6N2E2_389 0.61 +0.4 457,978 + Pf6N2E2_389 0.63 -0.0 457,979 - Pf6N2E2_389 0.63 -0.9 457,979 - Pf6N2E2_389 0.63 +0.3 458,183 + Pf6N2E2_389 0.76 -1.1 458,183 + Pf6N2E2_389 0.76 -0.4 458,183 + Pf6N2E2_389 0.76 +0.8 458,538 - +0.4 458,538 - +1.8 458,794 - Pf6N2E2_390 0.22 +0.4 458,794 - Pf6N2E2_390 0.22 +2.9 458,794 - Pf6N2E2_390 0.22 +0.1 458,943 + Pf6N2E2_390 0.42 -0.0 458,944 - Pf6N2E2_390 0.42 -1.1 459,054 + Pf6N2E2_390 0.56 -0.2 459,054 + Pf6N2E2_390 0.56 -0.2 459,054 + Pf6N2E2_390 0.56 +1.1 459,055 - Pf6N2E2_390 0.56 -0.4 459,288 - Pf6N2E2_390 0.86 -0.7 459,288 - Pf6N2E2_390 0.86 +0.2 459,310 + Pf6N2E2_390 0.89 +0.6 459,310 + Pf6N2E2_390 0.89 +0.6 459,310 + Pf6N2E2_390 0.89 -1.9 459,311 - Pf6N2E2_390 0.89 +0.3 459,311 - Pf6N2E2_390 0.89 +0.5 459,311 - Pf6N2E2_390 0.89 +1.3 459,311 - Pf6N2E2_390 0.89 -1.1 459,311 - Pf6N2E2_390 0.89 +0.6 459,459 + -1.2
Or see this region's nucleotide sequence