Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3883 and Pf6N2E2_3884 are separated by 37 nucleotides Pf6N2E2_3884 and Pf6N2E2_3885 are separated by 126 nucleotides Pf6N2E2_3885 and Pf6N2E2_3886 overlap by 4 nucleotides Pf6N2E2_3886 and Pf6N2E2_3887 are separated by 88 nucleotides
Pf6N2E2_3883 - C4-dicarboxylate transport transcriptional regulatory protein, at 4,516,032 to 4,517,423
_3883
Pf6N2E2_3884 - Histone acetyltransferase HPA2 and related acetyltransferases, at 4,517,461 to 4,517,916
_3884
Pf6N2E2_3885 - 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7), at 4,518,043 to 4,518,870
_3885
Pf6N2E2_3886 - ADP compounds hydrolase NudE (EC 3.6.1.-), at 4,518,867 to 4,519,433
_3886
Pf6N2E2_3887 - FIG001957: putative hydrolase, at 4,519,522 to 4,520,184
_3887
Position (kb)
4518
4519 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4517.362 kb on + strand at 4517.362 kb on + strand at 4517.363 kb on - strand at 4517.363 kb on - strand at 4517.427 kb on - strand at 4517.427 kb on - strand at 4517.427 kb on - strand at 4517.479 kb on + strand at 4517.561 kb on + strand, within Pf6N2E2_3884 at 4517.561 kb on + strand, within Pf6N2E2_3884 at 4517.561 kb on + strand, within Pf6N2E2_3884 at 4517.561 kb on + strand, within Pf6N2E2_3884 at 4517.562 kb on - strand, within Pf6N2E2_3884 at 4517.562 kb on - strand, within Pf6N2E2_3884 at 4517.562 kb on - strand, within Pf6N2E2_3884 at 4517.562 kb on - strand, within Pf6N2E2_3884 at 4517.562 kb on - strand, within Pf6N2E2_3884 at 4517.626 kb on + strand, within Pf6N2E2_3884 at 4517.626 kb on + strand, within Pf6N2E2_3884 at 4517.627 kb on - strand, within Pf6N2E2_3884 at 4517.700 kb on + strand, within Pf6N2E2_3884 at 4517.700 kb on + strand, within Pf6N2E2_3884 at 4517.729 kb on - strand, within Pf6N2E2_3884 at 4517.810 kb on - strand, within Pf6N2E2_3884 at 4517.810 kb on - strand, within Pf6N2E2_3884 at 4517.899 kb on + strand at 4517.899 kb on + strand at 4517.900 kb on - strand at 4517.900 kb on - strand at 4517.900 kb on - strand at 4518.038 kb on - strand at 4518.038 kb on - strand at 4518.064 kb on + strand at 4518.064 kb on + strand at 4518.064 kb on + strand at 4518.064 kb on + strand at 4518.065 kb on - strand at 4518.065 kb on - strand at 4518.111 kb on + strand at 4518.112 kb on - strand at 4518.112 kb on - strand at 4518.112 kb on - strand at 4518.190 kb on + strand, within Pf6N2E2_3885 at 4518.447 kb on + strand, within Pf6N2E2_3885 at 4518.448 kb on - strand, within Pf6N2E2_3885 at 4518.485 kb on + strand, within Pf6N2E2_3885 at 4518.838 kb on + strand at 4518.839 kb on - strand at 4518.914 kb on - strand at 4519.037 kb on - strand, within Pf6N2E2_3886 at 4519.078 kb on + strand, within Pf6N2E2_3886 at 4519.237 kb on + strand, within Pf6N2E2_3886 at 4519.237 kb on + strand, within Pf6N2E2_3886 at 4519.237 kb on + strand, within Pf6N2E2_3886 at 4519.238 kb on - strand, within Pf6N2E2_3886 at 4519.298 kb on - strand, within Pf6N2E2_3886 at 4519.327 kb on + strand, within Pf6N2E2_3886 at 4519.327 kb on + strand, within Pf6N2E2_3886 at 4519.327 kb on + strand, within Pf6N2E2_3886 at 4519.327 kb on + strand, within Pf6N2E2_3886 at 4519.327 kb on + strand, within Pf6N2E2_3886 at 4519.327 kb on + strand, within Pf6N2E2_3886 at 4519.327 kb on + strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.328 kb on - strand, within Pf6N2E2_3886 at 4519.413 kb on + strand at 4519.414 kb on - strand at 4519.414 kb on - strand at 4519.434 kb on - strand at 4519.463 kb on + strand at 4519.463 kb on + strand at 4519.463 kb on + strand at 4519.463 kb on + strand at 4519.463 kb on + strand at 4519.463 kb on + strand at 4519.464 kb on - strand at 4519.464 kb on - strand at 4519.464 kb on - strand at 4519.464 kb on - strand at 4519.464 kb on - strand at 4519.464 kb on - strand at 4519.465 kb on + strand at 4519.474 kb on + strand at 4519.522 kb on - strand at 4519.594 kb on + strand, within Pf6N2E2_3887 at 4519.594 kb on + strand, within Pf6N2E2_3887 at 4519.594 kb on + strand, within Pf6N2E2_3887 at 4519.594 kb on + strand, within Pf6N2E2_3887 at 4519.720 kb on + strand, within Pf6N2E2_3887 at 4519.720 kb on + strand, within Pf6N2E2_3887 at 4519.720 kb on + strand, within Pf6N2E2_3887 at 4519.721 kb on - strand, within Pf6N2E2_3887 at 4519.815 kb on + strand, within Pf6N2E2_3887 at 4519.816 kb on - strand, within Pf6N2E2_3887
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 4,517,362 + +0.6 4,517,362 + +0.6 4,517,363 - -0.1 4,517,363 - -1.6 4,517,427 - -0.1 4,517,427 - +0.3 4,517,427 - -0.1 4,517,479 + +0.1 4,517,561 + Pf6N2E2_3884 0.22 +0.0 4,517,561 + Pf6N2E2_3884 0.22 +0.2 4,517,561 + Pf6N2E2_3884 0.22 -0.1 4,517,561 + Pf6N2E2_3884 0.22 +0.0 4,517,562 - Pf6N2E2_3884 0.22 +0.0 4,517,562 - Pf6N2E2_3884 0.22 +0.5 4,517,562 - Pf6N2E2_3884 0.22 +0.9 4,517,562 - Pf6N2E2_3884 0.22 +1.6 4,517,562 - Pf6N2E2_3884 0.22 +0.1 4,517,626 + Pf6N2E2_3884 0.36 +0.7 4,517,626 + Pf6N2E2_3884 0.36 -0.0 4,517,627 - Pf6N2E2_3884 0.36 +0.4 4,517,700 + Pf6N2E2_3884 0.52 +2.5 4,517,700 + Pf6N2E2_3884 0.52 +0.3 4,517,729 - Pf6N2E2_3884 0.59 -0.1 4,517,810 - Pf6N2E2_3884 0.77 +1.0 4,517,810 - Pf6N2E2_3884 0.77 +1.1 4,517,899 + +0.4 4,517,899 + +1.2 4,517,900 - +0.5 4,517,900 - +0.2 4,517,900 - +0.4 4,518,038 - +0.0 4,518,038 - -0.4 4,518,064 + +1.2 4,518,064 + -0.0 4,518,064 + -0.6 4,518,064 + -0.3 4,518,065 - -0.1 4,518,065 - +1.1 4,518,111 + -0.3 4,518,112 - -0.0 4,518,112 - -0.9 4,518,112 - -1.4 4,518,190 + Pf6N2E2_3885 0.18 -1.0 4,518,447 + Pf6N2E2_3885 0.49 -1.8 4,518,448 - Pf6N2E2_3885 0.49 -0.2 4,518,485 + Pf6N2E2_3885 0.53 +0.4 4,518,838 + +1.0 4,518,839 - -0.9 4,518,914 - +1.3 4,519,037 - Pf6N2E2_3886 0.30 -0.2 4,519,078 + Pf6N2E2_3886 0.37 -0.6 4,519,237 + Pf6N2E2_3886 0.65 +1.3 4,519,237 + Pf6N2E2_3886 0.65 +2.3 4,519,237 + Pf6N2E2_3886 0.65 -0.9 4,519,238 - Pf6N2E2_3886 0.65 +0.1 4,519,298 - Pf6N2E2_3886 0.76 +0.4 4,519,327 + Pf6N2E2_3886 0.81 -1.4 4,519,327 + Pf6N2E2_3886 0.81 +0.3 4,519,327 + Pf6N2E2_3886 0.81 +0.6 4,519,327 + Pf6N2E2_3886 0.81 +1.1 4,519,327 + Pf6N2E2_3886 0.81 +0.2 4,519,327 + Pf6N2E2_3886 0.81 -2.0 4,519,327 + Pf6N2E2_3886 0.81 -1.2 4,519,328 - Pf6N2E2_3886 0.81 -0.7 4,519,328 - Pf6N2E2_3886 0.81 -0.0 4,519,328 - Pf6N2E2_3886 0.81 +1.1 4,519,328 - Pf6N2E2_3886 0.81 +0.4 4,519,328 - Pf6N2E2_3886 0.81 +0.2 4,519,328 - Pf6N2E2_3886 0.81 +0.9 4,519,328 - Pf6N2E2_3886 0.81 +0.6 4,519,328 - Pf6N2E2_3886 0.81 -2.0 4,519,328 - Pf6N2E2_3886 0.81 +0.6 4,519,328 - Pf6N2E2_3886 0.81 +1.0 4,519,328 - Pf6N2E2_3886 0.81 +0.6 4,519,413 + -0.3 4,519,414 - -0.6 4,519,414 - -0.1 4,519,434 - +1.4 4,519,463 + -0.9 4,519,463 + -0.0 4,519,463 + -0.3 4,519,463 + -0.4 4,519,463 + -0.4 4,519,463 + +1.1 4,519,464 - +0.1 4,519,464 - -0.0 4,519,464 - -0.1 4,519,464 - +0.4 4,519,464 - +0.6 4,519,464 - +0.6 4,519,465 + -0.8 4,519,474 + -0.6 4,519,522 - -1.3 4,519,594 + Pf6N2E2_3887 0.11 +1.1 4,519,594 + Pf6N2E2_3887 0.11 +0.9 4,519,594 + Pf6N2E2_3887 0.11 -0.0 4,519,594 + Pf6N2E2_3887 0.11 -0.4 4,519,720 + Pf6N2E2_3887 0.30 -0.5 4,519,720 + Pf6N2E2_3887 0.30 +0.6 4,519,720 + Pf6N2E2_3887 0.30 -0.0 4,519,721 - Pf6N2E2_3887 0.30 -0.1 4,519,815 + Pf6N2E2_3887 0.44 +0.3 4,519,816 - Pf6N2E2_3887 0.44 +0.0
Or see this region's nucleotide sequence