Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3781 and Pf6N2E2_3782 are separated by 112 nucleotides Pf6N2E2_3782 and Pf6N2E2_3783 overlap by 8 nucleotides Pf6N2E2_3783 and Pf6N2E2_3784 are separated by 24 nucleotides Pf6N2E2_3784 and Pf6N2E2_3785 are separated by 10 nucleotides
Pf6N2E2_3781 - Ubiquinone biosynthesis monooxygenase UbiB, at 4,403,801 to 4,405,405
_3781
Pf6N2E2_3782 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19), at 4,405,518 to 4,405,916
_3782
Pf6N2E2_3783 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 4,405,909 to 4,406,241
_3783
Pf6N2E2_3784 - Twin-arginine translocation protein TatA, at 4,406,266 to 4,406,553
_3784
Pf6N2E2_3785 - Twin-arginine translocation protein TatB, at 4,406,564 to 4,406,986
_3785
Position (kb)
4405
4406 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4405.449 kb on + strand at 4405.449 kb on + strand at 4405.449 kb on + strand at 4405.449 kb on + strand at 4405.449 kb on + strand at 4405.449 kb on + strand at 4405.449 kb on + strand at 4405.450 kb on - strand at 4405.450 kb on - strand at 4405.450 kb on - strand at 4405.450 kb on - strand at 4405.450 kb on - strand at 4405.450 kb on - strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.453 kb on + strand at 4405.454 kb on - strand at 4405.454 kb on - strand at 4405.454 kb on - strand at 4405.454 kb on - strand at 4405.454 kb on - strand at 4405.457 kb on + strand at 4405.457 kb on + strand at 4405.457 kb on + strand at 4405.457 kb on + strand at 4405.457 kb on + strand at 4405.457 kb on + strand at 4405.457 kb on + strand at 4405.457 kb on + strand at 4405.458 kb on - strand at 4405.458 kb on - strand at 4405.458 kb on - strand at 4405.488 kb on + strand at 4405.488 kb on + strand at 4405.665 kb on + strand, within Pf6N2E2_3782 at 4405.665 kb on + strand, within Pf6N2E2_3782 at 4405.665 kb on + strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.828 kb on - strand, within Pf6N2E2_3782 at 4405.893 kb on + strand at 4405.893 kb on + strand at 4405.894 kb on - strand at 4405.894 kb on - strand at 4405.904 kb on + strand at 4405.904 kb on + strand at 4405.904 kb on + strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.978 kb on + strand, within Pf6N2E2_3783 at 4405.979 kb on - strand, within Pf6N2E2_3783 at 4405.979 kb on - strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.102 kb on - strand, within Pf6N2E2_3783 at 4406.271 kb on + strand at 4406.562 kb on + strand at 4406.562 kb on + strand at 4406.562 kb on + strand at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.765 kb on - strand, within Pf6N2E2_3785
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 4,405,449 + -0.8 4,405,449 + +0.4 4,405,449 + +0.2 4,405,449 + -0.5 4,405,449 + -0.2 4,405,449 + -0.6 4,405,449 + -0.9 4,405,450 - -0.2 4,405,450 - -0.5 4,405,450 - +1.2 4,405,450 - -0.1 4,405,450 - -0.5 4,405,450 - +1.0 4,405,453 + +0.6 4,405,453 + +0.4 4,405,453 + +1.3 4,405,453 + -0.1 4,405,453 + +0.9 4,405,453 + +0.5 4,405,453 + +0.5 4,405,453 + +0.8 4,405,453 + +0.2 4,405,453 + +1.0 4,405,453 + -0.2 4,405,453 + +0.6 4,405,453 + +0.6 4,405,453 + +1.3 4,405,454 - +1.5 4,405,454 - -0.2 4,405,454 - -0.2 4,405,454 - +0.5 4,405,454 - -0.1 4,405,457 + +0.4 4,405,457 + +0.3 4,405,457 + +1.3 4,405,457 + +1.1 4,405,457 + -0.1 4,405,457 + -0.5 4,405,457 + +1.4 4,405,457 + +0.4 4,405,458 - -0.9 4,405,458 - -2.5 4,405,458 - -0.9 4,405,488 + +0.4 4,405,488 + -1.0 4,405,665 + Pf6N2E2_3782 0.37 +0.5 4,405,665 + Pf6N2E2_3782 0.37 +0.8 4,405,665 + Pf6N2E2_3782 0.37 +0.7 4,405,666 - Pf6N2E2_3782 0.37 -1.1 4,405,666 - Pf6N2E2_3782 0.37 -3.5 4,405,666 - Pf6N2E2_3782 0.37 -3.9 4,405,666 - Pf6N2E2_3782 0.37 -2.5 4,405,666 - Pf6N2E2_3782 0.37 -0.1 4,405,828 - Pf6N2E2_3782 0.78 -1.5 4,405,893 + +0.9 4,405,893 + +1.2 4,405,894 - +3.5 4,405,894 - +0.5 4,405,904 + -0.4 4,405,904 + +0.7 4,405,904 + -1.5 4,405,905 - +1.6 4,405,905 - +0.9 4,405,905 - +1.1 4,405,905 - +0.5 4,405,905 - +0.0 4,405,905 - -0.5 4,405,905 - -0.9 4,405,978 + Pf6N2E2_3783 0.21 -0.5 4,405,979 - Pf6N2E2_3783 0.21 -0.9 4,405,979 - Pf6N2E2_3783 0.21 -1.1 4,406,101 + Pf6N2E2_3783 0.58 -0.0 4,406,101 + Pf6N2E2_3783 0.58 -0.2 4,406,101 + Pf6N2E2_3783 0.58 -0.2 4,406,101 + Pf6N2E2_3783 0.58 +0.2 4,406,101 + Pf6N2E2_3783 0.58 -0.0 4,406,102 - Pf6N2E2_3783 0.58 -0.3 4,406,271 + -1.1 4,406,562 + -0.2 4,406,562 + -2.0 4,406,562 + -1.9 4,406,764 + Pf6N2E2_3785 0.47 -1.6 4,406,764 + Pf6N2E2_3785 0.47 -0.5 4,406,764 + Pf6N2E2_3785 0.47 +0.2 4,406,764 + Pf6N2E2_3785 0.47 -0.5 4,406,764 + Pf6N2E2_3785 0.47 -0.7 4,406,764 + Pf6N2E2_3785 0.47 -0.8 4,406,765 - Pf6N2E2_3785 0.48 -1.1
Or see this region's nucleotide sequence