Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3639 and Pf6N2E2_3640 are separated by 102 nucleotides Pf6N2E2_3640 and Pf6N2E2_3641 overlap by 10 nucleotides Pf6N2E2_3641 and Pf6N2E2_3642 are separated by 214 nucleotides Pf6N2E2_3642 and Pf6N2E2_3643 overlap by 32 nucleotides
Pf6N2E2_3639 - 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), at 4,239,075 to 4,239,911
_3639
Pf6N2E2_3640 - FIG074102: hypothetical protein, at 4,240,014 to 4,240,466
_3640
Pf6N2E2_3641 - ADP-ribose pyrophosphatase (EC 3.6.1.13), at 4,240,457 to 4,241,074
_3641
Pf6N2E2_3642 - lipoprotein, putative, at 4,241,289 to 4,242,035
_3642
Pf6N2E2_3643 - Predicted hydroxymethylpyrimidine transporter CytX, at 4,242,004 to 4,243,296
_3643
Position (kb)
4240
4241
4242 Strain fitness (log2 ratio)
-2
-1
0
1 at 4239.879 kb on + strand at 4239.879 kb on + strand at 4239.880 kb on - strand at 4239.880 kb on - strand at 4239.880 kb on - strand at 4239.880 kb on - strand at 4239.937 kb on + strand at 4239.937 kb on + strand at 4239.937 kb on + strand at 4239.937 kb on + strand at 4239.938 kb on - strand at 4239.938 kb on - strand at 4239.938 kb on - strand at 4239.938 kb on - strand at 4239.938 kb on - strand at 4240.001 kb on - strand at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.132 kb on + strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.133 kb on - strand, within Pf6N2E2_3640 at 4240.195 kb on + strand, within Pf6N2E2_3640 at 4240.196 kb on - strand, within Pf6N2E2_3640 at 4240.196 kb on - strand, within Pf6N2E2_3640 at 4240.196 kb on - strand, within Pf6N2E2_3640 at 4240.270 kb on + strand, within Pf6N2E2_3640 at 4240.393 kb on + strand, within Pf6N2E2_3640 at 4240.394 kb on - strand, within Pf6N2E2_3640 at 4240.394 kb on - strand, within Pf6N2E2_3640 at 4240.466 kb on + strand at 4240.592 kb on - strand, within Pf6N2E2_3641 at 4240.699 kb on - strand, within Pf6N2E2_3641 at 4240.699 kb on - strand, within Pf6N2E2_3641 at 4241.101 kb on + strand at 4241.101 kb on + strand at 4241.102 kb on - strand at 4241.102 kb on - strand at 4241.102 kb on - strand at 4241.204 kb on + strand at 4241.204 kb on + strand at 4241.205 kb on - strand at 4241.205 kb on - strand at 4241.205 kb on - strand at 4241.213 kb on + strand at 4241.213 kb on + strand at 4241.214 kb on - strand at 4241.367 kb on + strand, within Pf6N2E2_3642 at 4241.610 kb on + strand, within Pf6N2E2_3642 at 4241.611 kb on - strand, within Pf6N2E2_3642 at 4241.626 kb on + strand, within Pf6N2E2_3642 at 4241.627 kb on - strand, within Pf6N2E2_3642 at 4241.627 kb on - strand, within Pf6N2E2_3642 at 4241.627 kb on - strand, within Pf6N2E2_3642 at 4241.627 kb on - strand, within Pf6N2E2_3642 at 4241.670 kb on + strand, within Pf6N2E2_3642 at 4241.670 kb on + strand, within Pf6N2E2_3642 at 4241.671 kb on - strand, within Pf6N2E2_3642 at 4241.721 kb on + strand, within Pf6N2E2_3642 at 4241.925 kb on + strand, within Pf6N2E2_3642 at 4241.925 kb on + strand, within Pf6N2E2_3642 at 4241.926 kb on - strand, within Pf6N2E2_3642 at 4242.029 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 4,239,879 + +0.3 4,239,879 + -0.6 4,239,880 - +0.3 4,239,880 - -0.7 4,239,880 - +0.7 4,239,880 - -0.4 4,239,937 + +0.5 4,239,937 + -1.6 4,239,937 + +0.1 4,239,937 + +0.9 4,239,938 - -0.3 4,239,938 - -0.4 4,239,938 - +0.9 4,239,938 - -0.0 4,239,938 - +0.1 4,240,001 - +0.3 4,240,132 + Pf6N2E2_3640 0.26 +0.7 4,240,132 + Pf6N2E2_3640 0.26 -1.1 4,240,132 + Pf6N2E2_3640 0.26 -1.7 4,240,132 + Pf6N2E2_3640 0.26 -0.2 4,240,132 + Pf6N2E2_3640 0.26 -0.7 4,240,132 + Pf6N2E2_3640 0.26 +0.3 4,240,132 + Pf6N2E2_3640 0.26 -0.0 4,240,133 - Pf6N2E2_3640 0.26 -0.8 4,240,133 - Pf6N2E2_3640 0.26 +1.3 4,240,133 - Pf6N2E2_3640 0.26 -1.1 4,240,133 - Pf6N2E2_3640 0.26 -1.3 4,240,133 - Pf6N2E2_3640 0.26 +0.8 4,240,133 - Pf6N2E2_3640 0.26 -0.5 4,240,195 + Pf6N2E2_3640 0.40 +0.6 4,240,196 - Pf6N2E2_3640 0.40 +1.0 4,240,196 - Pf6N2E2_3640 0.40 +0.1 4,240,196 - Pf6N2E2_3640 0.40 +0.9 4,240,270 + Pf6N2E2_3640 0.57 -0.2 4,240,393 + Pf6N2E2_3640 0.84 -0.1 4,240,394 - Pf6N2E2_3640 0.84 +0.0 4,240,394 - Pf6N2E2_3640 0.84 +0.2 4,240,466 + -0.5 4,240,592 - Pf6N2E2_3641 0.22 -0.2 4,240,699 - Pf6N2E2_3641 0.39 -0.5 4,240,699 - Pf6N2E2_3641 0.39 -1.2 4,241,101 + -2.2 4,241,101 + -0.1 4,241,102 - +0.6 4,241,102 - -1.7 4,241,102 - +1.5 4,241,204 + +1.6 4,241,204 + +0.3 4,241,205 - -0.1 4,241,205 - -0.1 4,241,205 - +0.1 4,241,213 + -0.6 4,241,213 + -0.2 4,241,214 - +0.3 4,241,367 + Pf6N2E2_3642 0.10 -0.3 4,241,610 + Pf6N2E2_3642 0.43 -0.2 4,241,611 - Pf6N2E2_3642 0.43 +0.7 4,241,626 + Pf6N2E2_3642 0.45 +0.3 4,241,627 - Pf6N2E2_3642 0.45 +1.1 4,241,627 - Pf6N2E2_3642 0.45 -0.5 4,241,627 - Pf6N2E2_3642 0.45 +0.0 4,241,627 - Pf6N2E2_3642 0.45 -0.6 4,241,670 + Pf6N2E2_3642 0.51 -0.1 4,241,670 + Pf6N2E2_3642 0.51 +0.8 4,241,671 - Pf6N2E2_3642 0.51 +0.8 4,241,721 + Pf6N2E2_3642 0.58 -1.3 4,241,925 + Pf6N2E2_3642 0.85 +0.3 4,241,925 + Pf6N2E2_3642 0.85 -0.5 4,241,926 - Pf6N2E2_3642 0.85 -0.4 4,242,029 + -1.3
Or see this region's nucleotide sequence