Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3497 and Pf6N2E2_3498 overlap by 4 nucleotides Pf6N2E2_3498 and Pf6N2E2_3499 are separated by 164 nucleotides Pf6N2E2_3499 and Pf6N2E2_3500 are separated by 236 nucleotides
Pf6N2E2_3497 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 4,078,312 to 4,079,061
_3497
Pf6N2E2_3498 - FIG00953349: hypothetical protein, at 4,079,058 to 4,079,363
_3498
Pf6N2E2_3499 - Permeases of the major facilitator superfamily, at 4,079,528 to 4,079,821
_3499
Pf6N2E2_3500 - DNA-damage-inducible protein F, at 4,080,058 to 4,081,407
_3500
Position (kb)
4079
4080 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4078.608 kb on - strand, within Pf6N2E2_3497 at 4078.628 kb on + strand, within Pf6N2E2_3497 at 4078.629 kb on - strand, within Pf6N2E2_3497 at 4078.739 kb on + strand, within Pf6N2E2_3497 at 4078.739 kb on + strand, within Pf6N2E2_3497 at 4078.739 kb on + strand, within Pf6N2E2_3497 at 4078.740 kb on - strand, within Pf6N2E2_3497 at 4079.010 kb on - strand at 4079.149 kb on + strand, within Pf6N2E2_3498 at 4079.149 kb on + strand, within Pf6N2E2_3498 at 4079.150 kb on - strand, within Pf6N2E2_3498 at 4079.150 kb on - strand, within Pf6N2E2_3498 at 4079.384 kb on + strand at 4079.384 kb on + strand at 4079.385 kb on - strand at 4079.475 kb on + strand at 4079.727 kb on - strand, within Pf6N2E2_3499 at 4079.727 kb on - strand, within Pf6N2E2_3499 at 4079.727 kb on - strand, within Pf6N2E2_3499 at 4079.820 kb on - strand at 4079.820 kb on - strand at 4079.820 kb on - strand at 4079.869 kb on + strand at 4079.869 kb on + strand at 4079.869 kb on + strand at 4079.940 kb on + strand at 4079.940 kb on + strand at 4079.940 kb on + strand at 4079.940 kb on + strand at 4079.940 kb on + strand at 4079.940 kb on + strand at 4079.940 kb on + strand at 4079.940 kb on + strand at 4079.940 kb on + strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.941 kb on - strand at 4079.942 kb on + strand at 4079.942 kb on + strand at 4079.942 kb on + strand at 4079.943 kb on - strand at 4079.943 kb on - strand at 4079.943 kb on - strand at 4079.943 kb on - strand at 4079.943 kb on - strand at 4079.943 kb on - strand at 4079.971 kb on + strand at 4080.031 kb on - strand at 4080.174 kb on + strand at 4080.238 kb on + strand, within Pf6N2E2_3500 at 4080.239 kb on - strand, within Pf6N2E2_3500 at 4080.239 kb on - strand, within Pf6N2E2_3500 at 4080.239 kb on - strand, within Pf6N2E2_3500 at 4080.240 kb on + strand, within Pf6N2E2_3500 at 4080.378 kb on - strand, within Pf6N2E2_3500 at 4080.512 kb on - strand, within Pf6N2E2_3500 at 4080.512 kb on - strand, within Pf6N2E2_3500 at 4080.521 kb on + strand, within Pf6N2E2_3500 at 4080.521 kb on + strand, within Pf6N2E2_3500
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 4,078,608 - Pf6N2E2_3497 0.39 +0.4 4,078,628 + Pf6N2E2_3497 0.42 +0.5 4,078,629 - Pf6N2E2_3497 0.42 +0.1 4,078,739 + Pf6N2E2_3497 0.57 -0.2 4,078,739 + Pf6N2E2_3497 0.57 +0.1 4,078,739 + Pf6N2E2_3497 0.57 +0.1 4,078,740 - Pf6N2E2_3497 0.57 +0.8 4,079,010 - +0.2 4,079,149 + Pf6N2E2_3498 0.30 -1.7 4,079,149 + Pf6N2E2_3498 0.30 -0.8 4,079,150 - Pf6N2E2_3498 0.30 -0.0 4,079,150 - Pf6N2E2_3498 0.30 -0.7 4,079,384 + +2.0 4,079,384 + +1.1 4,079,385 - +0.8 4,079,475 + +0.7 4,079,727 - Pf6N2E2_3499 0.68 -0.1 4,079,727 - Pf6N2E2_3499 0.68 -2.1 4,079,727 - Pf6N2E2_3499 0.68 -0.8 4,079,820 - -0.6 4,079,820 - +0.7 4,079,820 - -0.7 4,079,869 + +0.7 4,079,869 + -0.8 4,079,869 + -0.2 4,079,940 + -0.1 4,079,940 + +1.0 4,079,940 + +0.1 4,079,940 + +0.5 4,079,940 + +0.1 4,079,940 + +1.3 4,079,940 + +0.8 4,079,940 + +1.4 4,079,940 + -0.0 4,079,941 - -0.2 4,079,941 - +0.4 4,079,941 - -0.1 4,079,941 - +0.2 4,079,941 - +0.6 4,079,941 - +0.4 4,079,941 - +0.2 4,079,941 - +0.5 4,079,941 - +0.8 4,079,941 - +0.4 4,079,941 - +0.1 4,079,942 + +0.8 4,079,942 + -0.5 4,079,942 + -0.4 4,079,943 - -0.9 4,079,943 - +1.1 4,079,943 - +0.4 4,079,943 - -1.5 4,079,943 - +0.3 4,079,943 - +1.5 4,079,971 + -0.3 4,080,031 - +0.4 4,080,174 + +0.2 4,080,238 + Pf6N2E2_3500 0.13 -1.8 4,080,239 - Pf6N2E2_3500 0.13 -1.6 4,080,239 - Pf6N2E2_3500 0.13 +0.4 4,080,239 - Pf6N2E2_3500 0.13 +0.1 4,080,240 + Pf6N2E2_3500 0.13 -0.9 4,080,378 - Pf6N2E2_3500 0.24 +0.9 4,080,512 - Pf6N2E2_3500 0.34 -0.5 4,080,512 - Pf6N2E2_3500 0.34 -0.6 4,080,521 + Pf6N2E2_3500 0.34 -1.0 4,080,521 + Pf6N2E2_3500 0.34 -1.0
Or see this region's nucleotide sequence