Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3476 and Pf6N2E2_3477 are separated by 84 nucleotides Pf6N2E2_3477 and Pf6N2E2_3478 are separated by 83 nucleotides
Pf6N2E2_3476 - Nucleoside ABC transporter, periplasmic nucleoside-binding protein, at 4,056,981 to 4,058,063
_3476
Pf6N2E2_3477 - 2-Oxobutyrate oxidase, putative, at 4,058,148 to 4,059,113
_3477
Pf6N2E2_3478 - Adenosine deaminase (EC 3.5.4.4), at 4,059,197 to 4,060,150
_3478
Position (kb)
4058
4059
4060 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4057.185 kb on + strand, within Pf6N2E2_3476 at 4057.185 kb on + strand, within Pf6N2E2_3476 at 4057.186 kb on - strand, within Pf6N2E2_3476 at 4057.186 kb on - strand, within Pf6N2E2_3476 at 4057.186 kb on - strand, within Pf6N2E2_3476 at 4057.186 kb on - strand, within Pf6N2E2_3476 at 4057.186 kb on - strand, within Pf6N2E2_3476 at 4057.186 kb on - strand, within Pf6N2E2_3476 at 4057.251 kb on + strand, within Pf6N2E2_3476 at 4057.252 kb on - strand, within Pf6N2E2_3476 at 4057.252 kb on - strand, within Pf6N2E2_3476 at 4057.252 kb on - strand, within Pf6N2E2_3476 at 4057.281 kb on + strand, within Pf6N2E2_3476 at 4057.347 kb on + strand, within Pf6N2E2_3476 at 4057.374 kb on + strand, within Pf6N2E2_3476 at 4057.389 kb on + strand, within Pf6N2E2_3476 at 4057.389 kb on + strand, within Pf6N2E2_3476 at 4057.389 kb on + strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.390 kb on - strand, within Pf6N2E2_3476 at 4057.456 kb on + strand, within Pf6N2E2_3476 at 4057.457 kb on - strand, within Pf6N2E2_3476 at 4057.457 kb on - strand, within Pf6N2E2_3476 at 4057.457 kb on - strand, within Pf6N2E2_3476 at 4057.521 kb on + strand, within Pf6N2E2_3476 at 4057.521 kb on + strand, within Pf6N2E2_3476 at 4057.521 kb on + strand, within Pf6N2E2_3476 at 4057.521 kb on + strand, within Pf6N2E2_3476 at 4057.522 kb on - strand, within Pf6N2E2_3476 at 4057.522 kb on - strand, within Pf6N2E2_3476 at 4057.522 kb on - strand, within Pf6N2E2_3476 at 4057.522 kb on - strand, within Pf6N2E2_3476 at 4057.522 kb on - strand, within Pf6N2E2_3476 at 4057.683 kb on + strand, within Pf6N2E2_3476 at 4057.684 kb on - strand, within Pf6N2E2_3476 at 4057.695 kb on + strand, within Pf6N2E2_3476 at 4057.767 kb on + strand, within Pf6N2E2_3476 at 4057.767 kb on + strand, within Pf6N2E2_3476 at 4057.767 kb on + strand, within Pf6N2E2_3476 at 4057.767 kb on + strand, within Pf6N2E2_3476 at 4057.768 kb on - strand, within Pf6N2E2_3476 at 4057.768 kb on - strand, within Pf6N2E2_3476 at 4057.818 kb on + strand, within Pf6N2E2_3476 at 4057.818 kb on + strand, within Pf6N2E2_3476 at 4058.025 kb on + strand at 4058.025 kb on + strand at 4058.025 kb on + strand at 4058.025 kb on + strand at 4058.025 kb on + strand at 4058.025 kb on + strand at 4058.028 kb on + strand at 4058.028 kb on + strand at 4058.028 kb on + strand at 4058.061 kb on + strand at 4058.062 kb on - strand at 4058.062 kb on - strand at 4058.086 kb on + strand at 4058.087 kb on - strand at 4058.087 kb on - strand at 4058.087 kb on - strand at 4058.087 kb on - strand at 4058.087 kb on - strand at 4058.127 kb on - strand at 4058.769 kb on + strand, within Pf6N2E2_3477 at 4058.896 kb on - strand, within Pf6N2E2_3477 at 4059.031 kb on - strand at 4059.031 kb on - strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.081 kb on + strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.082 kb on - strand at 4059.112 kb on - strand at 4059.159 kb on + strand at 4059.160 kb on - strand at 4059.200 kb on + strand at 4059.200 kb on + strand at 4059.200 kb on + strand at 4059.200 kb on + strand at 4059.200 kb on + strand at 4059.200 kb on + strand at 4059.200 kb on + strand at 4059.200 kb on + strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.201 kb on - strand at 4059.341 kb on + strand, within Pf6N2E2_3478 at 4059.342 kb on - strand, within Pf6N2E2_3478 at 4059.342 kb on - strand, within Pf6N2E2_3478 at 4059.342 kb on - strand, within Pf6N2E2_3478 at 4059.342 kb on - strand, within Pf6N2E2_3478 at 4059.377 kb on + strand, within Pf6N2E2_3478 at 4059.380 kb on + strand, within Pf6N2E2_3478 at 4059.380 kb on + strand, within Pf6N2E2_3478 at 4059.380 kb on + strand, within Pf6N2E2_3478 at 4059.381 kb on - strand, within Pf6N2E2_3478 at 4059.419 kb on + strand, within Pf6N2E2_3478 at 4059.419 kb on + strand, within Pf6N2E2_3478 at 4059.419 kb on + strand, within Pf6N2E2_3478 at 4059.437 kb on + strand, within Pf6N2E2_3478 at 4059.515 kb on - strand, within Pf6N2E2_3478 at 4059.803 kb on + strand, within Pf6N2E2_3478 at 4059.804 kb on - strand, within Pf6N2E2_3478 at 4060.070 kb on + strand at 4060.070 kb on + strand at 4060.070 kb on + strand at 4060.070 kb on + strand at 4060.071 kb on - strand at 4060.071 kb on - strand at 4060.071 kb on - strand at 4060.071 kb on - strand at 4060.072 kb on + strand at 4060.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 4,057,185 + Pf6N2E2_3476 0.19 +0.9 4,057,185 + Pf6N2E2_3476 0.19 +1.5 4,057,186 - Pf6N2E2_3476 0.19 +1.3 4,057,186 - Pf6N2E2_3476 0.19 +1.0 4,057,186 - Pf6N2E2_3476 0.19 +0.4 4,057,186 - Pf6N2E2_3476 0.19 +0.9 4,057,186 - Pf6N2E2_3476 0.19 +0.3 4,057,186 - Pf6N2E2_3476 0.19 -0.2 4,057,251 + Pf6N2E2_3476 0.25 +0.1 4,057,252 - Pf6N2E2_3476 0.25 +0.2 4,057,252 - Pf6N2E2_3476 0.25 +1.2 4,057,252 - Pf6N2E2_3476 0.25 +0.7 4,057,281 + Pf6N2E2_3476 0.28 +0.2 4,057,347 + Pf6N2E2_3476 0.34 -0.1 4,057,374 + Pf6N2E2_3476 0.36 +0.3 4,057,389 + Pf6N2E2_3476 0.38 +1.5 4,057,389 + Pf6N2E2_3476 0.38 -1.1 4,057,389 + Pf6N2E2_3476 0.38 +0.9 4,057,390 - Pf6N2E2_3476 0.38 -0.3 4,057,390 - Pf6N2E2_3476 0.38 +1.7 4,057,390 - Pf6N2E2_3476 0.38 +0.8 4,057,390 - Pf6N2E2_3476 0.38 -0.3 4,057,390 - Pf6N2E2_3476 0.38 -0.9 4,057,390 - Pf6N2E2_3476 0.38 +1.2 4,057,390 - Pf6N2E2_3476 0.38 +0.7 4,057,390 - Pf6N2E2_3476 0.38 +0.8 4,057,390 - Pf6N2E2_3476 0.38 +0.2 4,057,390 - Pf6N2E2_3476 0.38 -0.5 4,057,456 + Pf6N2E2_3476 0.44 +0.9 4,057,457 - Pf6N2E2_3476 0.44 -0.1 4,057,457 - Pf6N2E2_3476 0.44 +2.0 4,057,457 - Pf6N2E2_3476 0.44 -0.5 4,057,521 + Pf6N2E2_3476 0.50 -1.2 4,057,521 + Pf6N2E2_3476 0.50 +0.1 4,057,521 + Pf6N2E2_3476 0.50 +1.7 4,057,521 + Pf6N2E2_3476 0.50 -0.6 4,057,522 - Pf6N2E2_3476 0.50 +0.5 4,057,522 - Pf6N2E2_3476 0.50 -0.6 4,057,522 - Pf6N2E2_3476 0.50 +1.5 4,057,522 - Pf6N2E2_3476 0.50 -0.4 4,057,522 - Pf6N2E2_3476 0.50 +1.9 4,057,683 + Pf6N2E2_3476 0.65 +0.9 4,057,684 - Pf6N2E2_3476 0.65 -1.0 4,057,695 + Pf6N2E2_3476 0.66 -0.6 4,057,767 + Pf6N2E2_3476 0.73 -0.2 4,057,767 + Pf6N2E2_3476 0.73 +0.7 4,057,767 + Pf6N2E2_3476 0.73 +0.2 4,057,767 + Pf6N2E2_3476 0.73 +0.3 4,057,768 - Pf6N2E2_3476 0.73 +1.6 4,057,768 - Pf6N2E2_3476 0.73 +0.5 4,057,818 + Pf6N2E2_3476 0.77 +1.1 4,057,818 + Pf6N2E2_3476 0.77 +0.6 4,058,025 + +0.5 4,058,025 + +1.3 4,058,025 + +1.2 4,058,025 + -0.5 4,058,025 + -0.3 4,058,025 + +0.0 4,058,028 + +0.4 4,058,028 + +1.1 4,058,028 + -1.4 4,058,061 + +0.6 4,058,062 - +0.8 4,058,062 - -0.3 4,058,086 + -0.4 4,058,087 - -0.6 4,058,087 - +0.6 4,058,087 - -0.1 4,058,087 - -1.2 4,058,087 - -0.8 4,058,127 - +0.2 4,058,769 + Pf6N2E2_3477 0.64 -1.3 4,058,896 - Pf6N2E2_3477 0.77 -0.5 4,059,031 - +0.4 4,059,031 - +1.2 4,059,081 + +0.9 4,059,081 + +0.4 4,059,081 + -0.0 4,059,081 + -0.2 4,059,081 + +0.4 4,059,081 + -0.5 4,059,081 + -0.9 4,059,081 + +0.0 4,059,081 + -0.7 4,059,081 + +0.4 4,059,081 + +0.1 4,059,081 + +0.9 4,059,081 + -0.5 4,059,081 + +0.2 4,059,082 - -1.2 4,059,082 - +0.5 4,059,082 - -0.1 4,059,082 - +0.1 4,059,082 - -0.4 4,059,082 - +0.0 4,059,082 - -1.6 4,059,082 - +0.5 4,059,082 - -0.1 4,059,082 - +0.7 4,059,082 - +0.4 4,059,082 - -0.2 4,059,082 - -1.6 4,059,082 - -1.0 4,059,082 - +0.9 4,059,082 - -0.5 4,059,082 - +0.2 4,059,082 - +1.4 4,059,082 - +0.9 4,059,082 - +0.6 4,059,082 - -0.4 4,059,082 - -1.0 4,059,082 - -0.4 4,059,082 - -0.4 4,059,112 - -0.6 4,059,159 + -0.7 4,059,160 - -1.4 4,059,200 + -0.1 4,059,200 + +0.6 4,059,200 + -1.6 4,059,200 + +0.4 4,059,200 + +1.1 4,059,200 + -0.2 4,059,200 + +0.4 4,059,200 + -0.1 4,059,201 - +1.2 4,059,201 - +0.2 4,059,201 - +2.5 4,059,201 - -0.0 4,059,201 - +0.4 4,059,201 - +0.1 4,059,201 - +1.4 4,059,201 - +0.2 4,059,201 - +0.6 4,059,201 - -0.6 4,059,201 - +1.4 4,059,201 - +0.6 4,059,341 + Pf6N2E2_3478 0.15 +1.1 4,059,342 - Pf6N2E2_3478 0.15 +0.4 4,059,342 - Pf6N2E2_3478 0.15 +1.5 4,059,342 - Pf6N2E2_3478 0.15 +0.6 4,059,342 - Pf6N2E2_3478 0.15 -0.4 4,059,377 + Pf6N2E2_3478 0.19 +0.9 4,059,380 + Pf6N2E2_3478 0.19 -0.9 4,059,380 + Pf6N2E2_3478 0.19 +0.4 4,059,380 + Pf6N2E2_3478 0.19 -0.6 4,059,381 - Pf6N2E2_3478 0.19 -1.8 4,059,419 + Pf6N2E2_3478 0.23 +0.4 4,059,419 + Pf6N2E2_3478 0.23 -0.3 4,059,419 + Pf6N2E2_3478 0.23 +0.3 4,059,437 + Pf6N2E2_3478 0.25 -0.8 4,059,515 - Pf6N2E2_3478 0.33 -0.2 4,059,803 + Pf6N2E2_3478 0.64 +0.1 4,059,804 - Pf6N2E2_3478 0.64 +0.1 4,060,070 + +0.6 4,060,070 + -0.4 4,060,070 + -0.3 4,060,070 + -0.2 4,060,071 - +0.3 4,060,071 - +1.8 4,060,071 - +0.9 4,060,071 - -2.3 4,060,072 + -0.6 4,060,073 - +0.3
Or see this region's nucleotide sequence