Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_339 and Pf6N2E2_340 are separated by 1 nucleotides Pf6N2E2_340 and Pf6N2E2_341 are separated by 177 nucleotides Pf6N2E2_341 and Pf6N2E2_342 overlap by 4 nucleotides
Pf6N2E2_339 - Glutathione-regulated potassium-efflux system protein KefC, at 405,517 to 407,343
_339
Pf6N2E2_340 - Glutathione-regulated potassium-efflux system ancillary protein KefF, at 407,345 to 407,866
_340
Pf6N2E2_341 - sensor histidine kinase, at 408,044 to 409,390
_341
Pf6N2E2_342 - DNA-binding response regulator, at 409,387 to 410,097
_342
Position (kb) 408
409
410 Strain fitness (log2 ratio) -2
-1
0
1
2 at 407.188 kb on + strand at 407.189 kb on - strand at 407.189 kb on - strand at 407.239 kb on + strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.243 kb on + strand at 407.244 kb on - strand at 407.309 kb on + strand at 407.309 kb on + strand at 407.309 kb on + strand at 407.310 kb on - strand at 407.310 kb on - strand at 407.310 kb on - strand at 407.379 kb on + strand at 407.379 kb on + strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.421 kb on + strand, within Pf6N2E2_340 at 407.421 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.664 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.752 kb on - strand, within Pf6N2E2_340 at 407.760 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.838 kb on + strand at 407.838 kb on + strand at 407.850 kb on + strand at 407.851 kb on - strand at 407.851 kb on - strand at 407.940 kb on + strand at 408.018 kb on + strand at 408.316 kb on - strand, within Pf6N2E2_341 at 408.316 kb on - strand, within Pf6N2E2_341 at 408.316 kb on - strand, within Pf6N2E2_341 at 408.378 kb on + strand, within Pf6N2E2_341 at 408.378 kb on + strand, within Pf6N2E2_341 at 408.668 kb on + strand, within Pf6N2E2_341 at 409.011 kb on + strand, within Pf6N2E2_341 at 409.012 kb on - strand, within Pf6N2E2_341 at 409.805 kb on + strand, within Pf6N2E2_342 at 410.086 kb on + strand at 410.087 kb on - strand at 410.087 kb on - strand at 410.087 kb on - strand at 410.120 kb on + strand at 410.121 kb on - strand at 410.135 kb on + strand at 410.135 kb on + strand at 410.135 kb on + strand at 410.136 kb on - strand at 410.160 kb on + strand at 410.161 kb on - strand at 410.162 kb on + strand at 410.162 kb on + strand at 410.162 kb on + strand at 410.163 kb on - strand at 410.178 kb on + strand at 410.179 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 407,188 + +2.1 407,189 - -0.5 407,189 - -0.4 407,239 + +0.9 407,240 - -1.0 407,240 - +0.2 407,240 - +0.2 407,240 - +0.4 407,240 - -0.4 407,243 + +0.0 407,244 - -1.8 407,309 + +0.0 407,309 + +1.2 407,309 + -0.3 407,310 - +1.1 407,310 - +1.0 407,310 - +0.3 407,379 + -0.2 407,379 + +0.7 407,380 - -0.2 407,380 - -1.2 407,380 - +0.7 407,380 - +0.6 407,380 - +1.6 407,421 + Pf6N2E2_340 0.15 -0.2 407,421 + Pf6N2E2_340 0.15 -0.0 407,466 + Pf6N2E2_340 0.23 +0.4 407,466 + Pf6N2E2_340 0.23 -0.2 407,466 + Pf6N2E2_340 0.23 +0.9 407,664 + Pf6N2E2_340 0.61 +0.4 407,670 + Pf6N2E2_340 0.62 -0.8 407,670 + Pf6N2E2_340 0.62 +0.4 407,670 + Pf6N2E2_340 0.62 +0.4 407,752 - Pf6N2E2_340 0.78 +1.2 407,760 + Pf6N2E2_340 0.80 +0.9 407,771 + Pf6N2E2_340 0.82 +0.3 407,771 + Pf6N2E2_340 0.82 +0.4 407,771 + Pf6N2E2_340 0.82 -0.8 407,772 - Pf6N2E2_340 0.82 +0.5 407,772 - Pf6N2E2_340 0.82 +1.1 407,772 - Pf6N2E2_340 0.82 -0.5 407,838 + +0.7 407,838 + -0.4 407,850 + +1.9 407,851 - +0.6 407,851 - -0.8 407,940 + +0.8 408,018 + -0.2 408,316 - Pf6N2E2_341 0.20 +0.0 408,316 - Pf6N2E2_341 0.20 +1.1 408,316 - Pf6N2E2_341 0.20 -1.3 408,378 + Pf6N2E2_341 0.25 +0.4 408,378 + Pf6N2E2_341 0.25 -1.2 408,668 + Pf6N2E2_341 0.46 -0.2 409,011 + Pf6N2E2_341 0.72 -0.1 409,012 - Pf6N2E2_341 0.72 -2.2 409,805 + Pf6N2E2_342 0.59 +0.6 410,086 + -0.1 410,087 - +1.7 410,087 - -0.7 410,087 - -0.4 410,120 + +0.6 410,121 - -0.4 410,135 + -0.6 410,135 + +0.1 410,135 + +0.7 410,136 - -0.2 410,160 + +0.1 410,161 - -0.2 410,162 + +1.2 410,162 + +0.9 410,162 + -0.7 410,163 - -0.2 410,178 + +1.1 410,179 - +1.0
Or see this region's nucleotide sequence