Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3136 and Pf6N2E2_3137 are separated by 659 nucleotides Pf6N2E2_3137 and Pf6N2E2_3138 are separated by 158 nucleotides Pf6N2E2_3138 and Pf6N2E2_3139 are separated by 147 nucleotides
Pf6N2E2_3136 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 3,626,931 to 3,628,037
_3136
Pf6N2E2_3137 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 3,628,697 to 3,630,406
_3137
Pf6N2E2_3138 - YaeQ protein, at 3,630,565 to 3,631,107
_3138
Pf6N2E2_3139 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 3,631,255 to 3,632,454
_3139
Position (kb)
3628
3629
3630
3631 Strain fitness (log2 ratio)
-2
-1
0
1 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.745 kb on - strand, within Pf6N2E2_3136 at 3627.745 kb on - strand, within Pf6N2E2_3136 at 3627.958 kb on + strand at 3628.013 kb on + strand at 3628.015 kb on + strand at 3628.015 kb on + strand at 3628.015 kb on + strand at 3628.015 kb on + strand at 3628.016 kb on - strand at 3628.016 kb on - strand at 3628.016 kb on - strand at 3628.016 kb on - strand at 3628.169 kb on + strand at 3628.177 kb on - strand at 3628.212 kb on + strand at 3628.213 kb on - strand at 3628.770 kb on + strand at 3628.770 kb on + strand at 3628.770 kb on + strand at 3628.771 kb on - strand at 3629.154 kb on + strand, within Pf6N2E2_3137 at 3629.154 kb on + strand, within Pf6N2E2_3137 at 3629.154 kb on + strand, within Pf6N2E2_3137 at 3629.290 kb on - strand, within Pf6N2E2_3137 at 3629.290 kb on - strand, within Pf6N2E2_3137 at 3629.290 kb on - strand, within Pf6N2E2_3137 at 3629.290 kb on - strand, within Pf6N2E2_3137 at 3629.570 kb on - strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.777 kb on - strand, within Pf6N2E2_3137 at 3629.777 kb on - strand, within Pf6N2E2_3137 at 3629.784 kb on + strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3630.001 kb on + strand, within Pf6N2E2_3137 at 3630.002 kb on - strand, within Pf6N2E2_3137 at 3630.008 kb on - strand, within Pf6N2E2_3137 at 3630.081 kb on + strand, within Pf6N2E2_3137 at 3630.081 kb on + strand, within Pf6N2E2_3137 at 3630.082 kb on - strand, within Pf6N2E2_3137 at 3630.082 kb on - strand, within Pf6N2E2_3137 at 3630.105 kb on + strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.330 kb on + strand at 3630.331 kb on - strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.957 kb on - strand, within Pf6N2E2_3138 at 3630.968 kb on + strand, within Pf6N2E2_3138 at 3630.969 kb on - strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.208 kb on - strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.222 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 3,627,744 + Pf6N2E2_3136 0.73 +0.0 3,627,744 + Pf6N2E2_3136 0.73 -0.8 3,627,744 + Pf6N2E2_3136 0.73 +1.1 3,627,744 + Pf6N2E2_3136 0.73 -1.0 3,627,744 + Pf6N2E2_3136 0.73 +0.9 3,627,745 - Pf6N2E2_3136 0.74 -0.1 3,627,745 - Pf6N2E2_3136 0.74 -1.5 3,627,958 + +0.1 3,628,013 + -0.5 3,628,015 + -1.0 3,628,015 + -0.2 3,628,015 + -0.2 3,628,015 + -0.8 3,628,016 - +1.4 3,628,016 - +0.0 3,628,016 - -0.9 3,628,016 - +0.1 3,628,169 + -0.8 3,628,177 - +0.8 3,628,212 + -0.8 3,628,213 - -0.1 3,628,770 + +0.7 3,628,770 + -0.4 3,628,770 + +1.2 3,628,771 - -0.8 3,629,154 + Pf6N2E2_3137 0.27 +0.5 3,629,154 + Pf6N2E2_3137 0.27 -1.7 3,629,154 + Pf6N2E2_3137 0.27 +0.2 3,629,290 - Pf6N2E2_3137 0.35 +0.2 3,629,290 - Pf6N2E2_3137 0.35 -0.5 3,629,290 - Pf6N2E2_3137 0.35 -0.6 3,629,290 - Pf6N2E2_3137 0.35 +0.3 3,629,570 - Pf6N2E2_3137 0.51 -0.1 3,629,776 + Pf6N2E2_3137 0.63 -0.0 3,629,776 + Pf6N2E2_3137 0.63 +0.1 3,629,776 + Pf6N2E2_3137 0.63 +0.0 3,629,777 - Pf6N2E2_3137 0.63 -0.6 3,629,777 - Pf6N2E2_3137 0.63 -0.8 3,629,784 + Pf6N2E2_3137 0.64 +1.3 3,629,785 - Pf6N2E2_3137 0.64 -0.3 3,629,785 - Pf6N2E2_3137 0.64 +0.1 3,629,785 - Pf6N2E2_3137 0.64 +0.5 3,629,785 - Pf6N2E2_3137 0.64 -0.6 3,629,785 - Pf6N2E2_3137 0.64 +0.8 3,629,829 + Pf6N2E2_3137 0.66 +1.0 3,629,829 + Pf6N2E2_3137 0.66 +0.2 3,629,829 + Pf6N2E2_3137 0.66 -0.2 3,629,830 - Pf6N2E2_3137 0.66 -0.7 3,629,830 - Pf6N2E2_3137 0.66 -0.8 3,629,830 - Pf6N2E2_3137 0.66 -0.3 3,629,830 - Pf6N2E2_3137 0.66 -0.3 3,630,001 + Pf6N2E2_3137 0.76 +0.7 3,630,002 - Pf6N2E2_3137 0.76 +1.2 3,630,008 - Pf6N2E2_3137 0.77 +0.4 3,630,081 + Pf6N2E2_3137 0.81 -0.5 3,630,081 + Pf6N2E2_3137 0.81 +0.2 3,630,082 - Pf6N2E2_3137 0.81 -0.4 3,630,082 - Pf6N2E2_3137 0.81 -0.2 3,630,105 + Pf6N2E2_3137 0.82 -0.4 3,630,106 - Pf6N2E2_3137 0.82 +1.7 3,630,106 - Pf6N2E2_3137 0.82 -0.1 3,630,106 - Pf6N2E2_3137 0.82 -0.7 3,630,330 + +0.5 3,630,331 - -1.3 3,630,398 + +0.1 3,630,398 + -1.2 3,630,398 + -0.2 3,630,398 + +0.6 3,630,398 + -0.1 3,630,399 - +0.3 3,630,399 - -1.4 3,630,399 - +1.1 3,630,957 - Pf6N2E2_3138 0.72 -2.2 3,630,968 + Pf6N2E2_3138 0.74 +0.5 3,630,969 - Pf6N2E2_3138 0.74 +0.0 3,631,043 + Pf6N2E2_3138 0.88 +0.0 3,631,043 + Pf6N2E2_3138 0.88 +1.3 3,631,044 - Pf6N2E2_3138 0.88 -0.4 3,631,044 - Pf6N2E2_3138 0.88 -1.3 3,631,044 - Pf6N2E2_3138 0.88 +0.9 3,631,044 - Pf6N2E2_3138 0.88 -1.4 3,631,085 + +0.3 3,631,085 + +0.0 3,631,085 + +0.7 3,631,123 + +0.7 3,631,123 + -0.0 3,631,123 + +0.2 3,631,208 - +0.6 3,631,212 + +0.1 3,631,212 + -0.3 3,631,212 + +0.3 3,631,213 - +0.1 3,631,213 - +1.0 3,631,213 - -0.1 3,631,222 + -1.4
Or see this region's nucleotide sequence