Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2956 and Pf6N2E2_2957 overlap by 4 nucleotides Pf6N2E2_2957 and Pf6N2E2_2958 are separated by 362 nucleotides
Pf6N2E2_2956 - mRNA 3-end processing factor, at 3,446,985 to 3,448,004
_2956
Pf6N2E2_2957 - ATP-dependent DNA ligase (EC 6.5.1.1) LigC, at 3,448,001 to 3,449,689
_2957
Pf6N2E2_2958 - ABC transporter for L-Lysine, periplasmic substrate-binding component (from data) , at 3,450,052 to 3,450,834
_2958
Position (kb)
3448
3449
3450 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3447.005 kb on + strand at 3447.005 kb on + strand at 3447.006 kb on - strand at 3447.006 kb on - strand at 3447.006 kb on - strand at 3447.006 kb on - strand at 3447.027 kb on + strand at 3447.027 kb on + strand at 3447.028 kb on - strand at 3447.028 kb on - strand at 3447.260 kb on - strand, within Pf6N2E2_2956 at 3447.260 kb on - strand, within Pf6N2E2_2956 at 3447.260 kb on - strand, within Pf6N2E2_2956 at 3447.270 kb on + strand, within Pf6N2E2_2956 at 3447.270 kb on + strand, within Pf6N2E2_2956 at 3447.271 kb on - strand, within Pf6N2E2_2956 at 3447.271 kb on - strand, within Pf6N2E2_2956 at 3447.271 kb on - strand, within Pf6N2E2_2956 at 3447.303 kb on + strand, within Pf6N2E2_2956 at 3447.304 kb on - strand, within Pf6N2E2_2956 at 3447.304 kb on - strand, within Pf6N2E2_2956 at 3447.356 kb on + strand, within Pf6N2E2_2956 at 3447.356 kb on + strand, within Pf6N2E2_2956 at 3447.393 kb on + strand, within Pf6N2E2_2956 at 3447.393 kb on + strand, within Pf6N2E2_2956 at 3447.394 kb on - strand, within Pf6N2E2_2956 at 3447.394 kb on - strand, within Pf6N2E2_2956 at 3447.487 kb on - strand, within Pf6N2E2_2956 at 3447.487 kb on - strand, within Pf6N2E2_2956 at 3447.487 kb on - strand, within Pf6N2E2_2956 at 3447.619 kb on - strand, within Pf6N2E2_2956 at 3447.721 kb on - strand, within Pf6N2E2_2956 at 3447.945 kb on + strand at 3447.945 kb on + strand at 3447.946 kb on - strand at 3448.079 kb on + strand at 3448.079 kb on + strand at 3448.079 kb on + strand at 3448.080 kb on - strand at 3448.080 kb on - strand at 3448.080 kb on - strand at 3448.121 kb on + strand at 3448.121 kb on + strand at 3448.122 kb on - strand at 3448.122 kb on - strand at 3448.226 kb on + strand, within Pf6N2E2_2957 at 3448.227 kb on - strand, within Pf6N2E2_2957 at 3448.267 kb on - strand, within Pf6N2E2_2957 at 3448.314 kb on + strand, within Pf6N2E2_2957 at 3448.314 kb on + strand, within Pf6N2E2_2957 at 3448.314 kb on + strand, within Pf6N2E2_2957 at 3448.314 kb on + strand, within Pf6N2E2_2957 at 3448.315 kb on - strand, within Pf6N2E2_2957 at 3448.568 kb on + strand, within Pf6N2E2_2957 at 3448.568 kb on + strand, within Pf6N2E2_2957 at 3449.027 kb on + strand, within Pf6N2E2_2957 at 3449.028 kb on - strand, within Pf6N2E2_2957 at 3449.243 kb on + strand, within Pf6N2E2_2957 at 3449.324 kb on + strand, within Pf6N2E2_2957 at 3449.325 kb on - strand, within Pf6N2E2_2957 at 3449.325 kb on - strand, within Pf6N2E2_2957 at 3449.361 kb on - strand, within Pf6N2E2_2957 at 3449.370 kb on - strand, within Pf6N2E2_2957 at 3449.438 kb on + strand, within Pf6N2E2_2957 at 3449.439 kb on - strand, within Pf6N2E2_2957 at 3449.636 kb on - strand at 3449.656 kb on + strand at 3449.657 kb on - strand at 3449.657 kb on - strand at 3449.657 kb on - strand at 3449.657 kb on - strand at 3449.657 kb on - strand at 3449.687 kb on + strand at 3449.687 kb on + strand at 3449.687 kb on + strand at 3449.876 kb on + strand at 3449.876 kb on + strand at 3449.876 kb on + strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.965 kb on - strand at 3449.970 kb on + strand at 3449.970 kb on + strand at 3449.970 kb on + strand at 3449.980 kb on + strand at 3449.981 kb on - strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.011 kb on + strand at 3450.012 kb on - strand at 3450.012 kb on - strand at 3450.031 kb on - strand at 3450.031 kb on - strand at 3450.039 kb on + strand at 3450.050 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.125 kb on - strand at 3450.125 kb on - strand at 3450.158 kb on - strand, within Pf6N2E2_2958 at 3450.303 kb on + strand, within Pf6N2E2_2958 at 3450.303 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.398 kb on - strand, within Pf6N2E2_2958 at 3450.398 kb on - strand, within Pf6N2E2_2958 at 3450.490 kb on + strand, within Pf6N2E2_2958 at 3450.490 kb on + strand, within Pf6N2E2_2958 at 3450.491 kb on - strand, within Pf6N2E2_2958 at 3450.556 kb on + strand, within Pf6N2E2_2958 at 3450.649 kb on + strand, within Pf6N2E2_2958
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 3,447,005 + +1.6 3,447,005 + -0.0 3,447,006 - +1.8 3,447,006 - -0.5 3,447,006 - +1.2 3,447,006 - +0.4 3,447,027 + -0.4 3,447,027 + -0.2 3,447,028 - +0.7 3,447,028 - +0.6 3,447,260 - Pf6N2E2_2956 0.27 +2.1 3,447,260 - Pf6N2E2_2956 0.27 -0.0 3,447,260 - Pf6N2E2_2956 0.27 -1.1 3,447,270 + Pf6N2E2_2956 0.28 +1.9 3,447,270 + Pf6N2E2_2956 0.28 -0.4 3,447,271 - Pf6N2E2_2956 0.28 +0.9 3,447,271 - Pf6N2E2_2956 0.28 -1.1 3,447,271 - Pf6N2E2_2956 0.28 +0.1 3,447,303 + Pf6N2E2_2956 0.31 +0.3 3,447,304 - Pf6N2E2_2956 0.31 +0.7 3,447,304 - Pf6N2E2_2956 0.31 -1.5 3,447,356 + Pf6N2E2_2956 0.36 +0.2 3,447,356 + Pf6N2E2_2956 0.36 -0.2 3,447,393 + Pf6N2E2_2956 0.40 -0.3 3,447,393 + Pf6N2E2_2956 0.40 -0.4 3,447,394 - Pf6N2E2_2956 0.40 +0.6 3,447,394 - Pf6N2E2_2956 0.40 +2.4 3,447,487 - Pf6N2E2_2956 0.49 +0.4 3,447,487 - Pf6N2E2_2956 0.49 +0.8 3,447,487 - Pf6N2E2_2956 0.49 +0.4 3,447,619 - Pf6N2E2_2956 0.62 +1.2 3,447,721 - Pf6N2E2_2956 0.72 +0.5 3,447,945 + -0.8 3,447,945 + -0.1 3,447,946 - +0.7 3,448,079 + +1.3 3,448,079 + +0.5 3,448,079 + -1.2 3,448,080 - +0.2 3,448,080 - -0.8 3,448,080 - +1.6 3,448,121 + +1.3 3,448,121 + -0.3 3,448,122 - +0.4 3,448,122 - +1.1 3,448,226 + Pf6N2E2_2957 0.13 +0.5 3,448,227 - Pf6N2E2_2957 0.13 -0.9 3,448,267 - Pf6N2E2_2957 0.16 -0.9 3,448,314 + Pf6N2E2_2957 0.19 +0.2 3,448,314 + Pf6N2E2_2957 0.19 -0.1 3,448,314 + Pf6N2E2_2957 0.19 +0.8 3,448,314 + Pf6N2E2_2957 0.19 -0.1 3,448,315 - Pf6N2E2_2957 0.19 -1.0 3,448,568 + Pf6N2E2_2957 0.34 +1.6 3,448,568 + Pf6N2E2_2957 0.34 +0.1 3,449,027 + Pf6N2E2_2957 0.61 +0.4 3,449,028 - Pf6N2E2_2957 0.61 +0.1 3,449,243 + Pf6N2E2_2957 0.74 +0.9 3,449,324 + Pf6N2E2_2957 0.78 +0.3 3,449,325 - Pf6N2E2_2957 0.78 +1.1 3,449,325 - Pf6N2E2_2957 0.78 +0.6 3,449,361 - Pf6N2E2_2957 0.81 +0.1 3,449,370 - Pf6N2E2_2957 0.81 +2.3 3,449,438 + Pf6N2E2_2957 0.85 -0.2 3,449,439 - Pf6N2E2_2957 0.85 -0.1 3,449,636 - +0.1 3,449,656 + +0.8 3,449,657 - +1.4 3,449,657 - -1.3 3,449,657 - -0.3 3,449,657 - -0.6 3,449,657 - +0.4 3,449,687 + -0.3 3,449,687 + -0.3 3,449,687 + -0.3 3,449,876 + -0.4 3,449,876 + -0.3 3,449,876 + -0.3 3,449,877 - -0.5 3,449,877 - +0.3 3,449,877 - +0.7 3,449,877 - -0.7 3,449,877 - +1.0 3,449,877 - +0.3 3,449,877 - +0.1 3,449,877 - -0.9 3,449,877 - -0.4 3,449,877 - -0.5 3,449,877 - -0.2 3,449,965 - -0.1 3,449,970 + -0.7 3,449,970 + +2.1 3,449,970 + +0.7 3,449,980 + -0.1 3,449,981 - -0.3 3,450,007 + +0.4 3,450,007 + -0.0 3,450,007 + +0.4 3,450,007 + +1.5 3,450,007 + +0.6 3,450,007 + +0.3 3,450,007 + +0.7 3,450,008 - +0.9 3,450,008 - +0.2 3,450,008 - -0.2 3,450,008 - +0.7 3,450,008 - +0.5 3,450,008 - +0.7 3,450,011 + -0.6 3,450,012 - +2.1 3,450,012 - -1.0 3,450,031 - -0.0 3,450,031 - -0.4 3,450,039 + -1.9 3,450,050 + +0.5 3,450,124 + +1.3 3,450,124 + +0.5 3,450,124 + -0.9 3,450,124 + +0.8 3,450,124 + +0.9 3,450,124 + -1.2 3,450,125 - -0.1 3,450,125 - +1.0 3,450,158 - Pf6N2E2_2958 0.14 +0.9 3,450,303 + Pf6N2E2_2958 0.32 +0.9 3,450,303 + Pf6N2E2_2958 0.32 +0.2 3,450,397 + Pf6N2E2_2958 0.44 -0.3 3,450,397 + Pf6N2E2_2958 0.44 -0.2 3,450,397 + Pf6N2E2_2958 0.44 +0.1 3,450,397 + Pf6N2E2_2958 0.44 +0.9 3,450,397 + Pf6N2E2_2958 0.44 +0.5 3,450,397 + Pf6N2E2_2958 0.44 +0.2 3,450,397 + Pf6N2E2_2958 0.44 +0.5 3,450,398 - Pf6N2E2_2958 0.44 -0.2 3,450,398 - Pf6N2E2_2958 0.44 +1.4 3,450,490 + Pf6N2E2_2958 0.56 -0.5 3,450,490 + Pf6N2E2_2958 0.56 +0.7 3,450,491 - Pf6N2E2_2958 0.56 -0.1 3,450,556 + Pf6N2E2_2958 0.64 -0.8 3,450,649 + Pf6N2E2_2958 0.76 -0.5
Or see this region's nucleotide sequence