Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2862 and Pf6N2E2_2863 are separated by 155 nucleotides Pf6N2E2_2863 and Pf6N2E2_2864 are separated by 92 nucleotides Pf6N2E2_2864 and Pf6N2E2_2865 are separated by 216 nucleotides
Pf6N2E2_2862 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 3,351,330 to 3,352,547
_2862
Pf6N2E2_2863 - Gluconate permease, at 3,352,703 to 3,354,055
_2863
Pf6N2E2_2864 - Gluconokinase (EC 2.7.1.12), at 3,354,148 to 3,354,681
_2864
Pf6N2E2_2865 - Gluconate utilization system Gnt-I transcriptional repressor, at 3,354,898 to 3,355,929
_2865
Position (kb)
3352
3353
3354
3355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3351.738 kb on + strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.936 kb on + strand, within Pf6N2E2_2862 at 3351.937 kb on - strand, within Pf6N2E2_2862 at 3352.065 kb on + strand, within Pf6N2E2_2862 at 3352.065 kb on + strand, within Pf6N2E2_2862 at 3352.066 kb on - strand, within Pf6N2E2_2862 at 3352.066 kb on - strand, within Pf6N2E2_2862 at 3352.143 kb on + strand, within Pf6N2E2_2862 at 3352.143 kb on + strand, within Pf6N2E2_2862 at 3352.143 kb on + strand, within Pf6N2E2_2862 at 3352.144 kb on - strand, within Pf6N2E2_2862 at 3352.152 kb on + strand, within Pf6N2E2_2862 at 3352.255 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.315 kb on - strand, within Pf6N2E2_2862 at 3352.341 kb on + strand, within Pf6N2E2_2862 at 3352.341 kb on + strand, within Pf6N2E2_2862 at 3352.341 kb on + strand, within Pf6N2E2_2862 at 3352.342 kb on - strand, within Pf6N2E2_2862 at 3352.342 kb on - strand, within Pf6N2E2_2862 at 3352.429 kb on - strand at 3352.704 kb on + strand at 3352.705 kb on - strand at 3352.730 kb on - strand at 3352.809 kb on + strand at 3352.809 kb on + strand at 3352.809 kb on + strand at 3352.810 kb on - strand at 3352.810 kb on - strand at 3352.810 kb on - strand at 3352.945 kb on + strand, within Pf6N2E2_2863 at 3353.199 kb on + strand, within Pf6N2E2_2863 at 3353.200 kb on - strand, within Pf6N2E2_2863 at 3353.200 kb on - strand, within Pf6N2E2_2863 at 3353.450 kb on - strand, within Pf6N2E2_2863 at 3353.502 kb on + strand, within Pf6N2E2_2863 at 3353.503 kb on - strand, within Pf6N2E2_2863 at 3353.503 kb on - strand, within Pf6N2E2_2863 at 3353.534 kb on + strand, within Pf6N2E2_2863 at 3353.534 kb on + strand, within Pf6N2E2_2863 at 3353.534 kb on + strand, within Pf6N2E2_2863 at 3353.534 kb on + strand, within Pf6N2E2_2863 at 3353.534 kb on + strand, within Pf6N2E2_2863 at 3353.534 kb on + strand, within Pf6N2E2_2863 at 3353.534 kb on + strand, within Pf6N2E2_2863 at 3353.535 kb on - strand, within Pf6N2E2_2863 at 3353.535 kb on - strand, within Pf6N2E2_2863 at 3353.535 kb on - strand, within Pf6N2E2_2863 at 3353.709 kb on - strand, within Pf6N2E2_2863 at 3353.862 kb on - strand, within Pf6N2E2_2863 at 3354.083 kb on + strand at 3354.188 kb on + strand at 3354.188 kb on + strand at 3354.188 kb on + strand at 3354.188 kb on + strand at 3354.188 kb on + strand at 3354.189 kb on - strand at 3354.189 kb on - strand at 3354.189 kb on - strand at 3354.189 kb on - strand at 3354.411 kb on - strand, within Pf6N2E2_2864 at 3354.608 kb on - strand, within Pf6N2E2_2864 at 3354.684 kb on - strand at 3354.721 kb on + strand at 3354.722 kb on - strand at 3354.776 kb on + strand at 3354.829 kb on + strand at 3354.829 kb on + strand at 3354.829 kb on + strand at 3354.830 kb on - strand at 3354.830 kb on - strand at 3354.830 kb on - strand at 3354.830 kb on - strand at 3354.830 kb on - strand at 3354.830 kb on - strand at 3354.830 kb on - strand at 3354.864 kb on + strand at 3354.865 kb on - strand at 3354.865 kb on - strand at 3354.867 kb on + strand at 3354.867 kb on + strand at 3354.924 kb on + strand at 3354.925 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 3,351,738 + Pf6N2E2_2862 0.33 -0.8 3,351,739 - Pf6N2E2_2862 0.34 +0.9 3,351,739 - Pf6N2E2_2862 0.34 +0.6 3,351,739 - Pf6N2E2_2862 0.34 -1.6 3,351,936 + Pf6N2E2_2862 0.50 +1.0 3,351,937 - Pf6N2E2_2862 0.50 +0.4 3,352,065 + Pf6N2E2_2862 0.60 +0.3 3,352,065 + Pf6N2E2_2862 0.60 +0.4 3,352,066 - Pf6N2E2_2862 0.60 -0.2 3,352,066 - Pf6N2E2_2862 0.60 +0.5 3,352,143 + Pf6N2E2_2862 0.67 +0.9 3,352,143 + Pf6N2E2_2862 0.67 +1.5 3,352,143 + Pf6N2E2_2862 0.67 +0.3 3,352,144 - Pf6N2E2_2862 0.67 -1.2 3,352,152 + Pf6N2E2_2862 0.67 -1.2 3,352,255 + Pf6N2E2_2862 0.76 +0.0 3,352,314 + Pf6N2E2_2862 0.81 -0.3 3,352,314 + Pf6N2E2_2862 0.81 +1.0 3,352,314 + Pf6N2E2_2862 0.81 -0.0 3,352,314 + Pf6N2E2_2862 0.81 +1.1 3,352,314 + Pf6N2E2_2862 0.81 -0.4 3,352,314 + Pf6N2E2_2862 0.81 -0.2 3,352,314 + Pf6N2E2_2862 0.81 -0.1 3,352,315 - Pf6N2E2_2862 0.81 +1.1 3,352,341 + Pf6N2E2_2862 0.83 -0.4 3,352,341 + Pf6N2E2_2862 0.83 +1.9 3,352,341 + Pf6N2E2_2862 0.83 +0.9 3,352,342 - Pf6N2E2_2862 0.83 +0.0 3,352,342 - Pf6N2E2_2862 0.83 +0.0 3,352,429 - -0.2 3,352,704 + +0.3 3,352,705 - +1.9 3,352,730 - -1.5 3,352,809 + -1.0 3,352,809 + -0.6 3,352,809 + -1.0 3,352,810 - -1.0 3,352,810 - -0.4 3,352,810 - +0.2 3,352,945 + Pf6N2E2_2863 0.18 -1.0 3,353,199 + Pf6N2E2_2863 0.37 -2.1 3,353,200 - Pf6N2E2_2863 0.37 -1.1 3,353,200 - Pf6N2E2_2863 0.37 -1.6 3,353,450 - Pf6N2E2_2863 0.55 -0.7 3,353,502 + Pf6N2E2_2863 0.59 -2.2 3,353,503 - Pf6N2E2_2863 0.59 +0.4 3,353,503 - Pf6N2E2_2863 0.59 -0.1 3,353,534 + Pf6N2E2_2863 0.61 -3.3 3,353,534 + Pf6N2E2_2863 0.61 -1.3 3,353,534 + Pf6N2E2_2863 0.61 -1.8 3,353,534 + Pf6N2E2_2863 0.61 -2.0 3,353,534 + Pf6N2E2_2863 0.61 +0.1 3,353,534 + Pf6N2E2_2863 0.61 -2.6 3,353,534 + Pf6N2E2_2863 0.61 -0.8 3,353,535 - Pf6N2E2_2863 0.61 -1.1 3,353,535 - Pf6N2E2_2863 0.61 -1.9 3,353,535 - Pf6N2E2_2863 0.61 -2.4 3,353,709 - Pf6N2E2_2863 0.74 -0.4 3,353,862 - Pf6N2E2_2863 0.86 -1.0 3,354,083 + -0.9 3,354,188 + -2.1 3,354,188 + -0.1 3,354,188 + -0.4 3,354,188 + -0.4 3,354,188 + +0.0 3,354,189 - -2.0 3,354,189 - -0.7 3,354,189 - -2.0 3,354,189 - +0.4 3,354,411 - Pf6N2E2_2864 0.49 -0.7 3,354,608 - Pf6N2E2_2864 0.86 -1.0 3,354,684 - +1.0 3,354,721 + -1.0 3,354,722 - +0.3 3,354,776 + +0.0 3,354,829 + -0.5 3,354,829 + +0.4 3,354,829 + -1.5 3,354,830 - -0.2 3,354,830 - +1.7 3,354,830 - +1.0 3,354,830 - +1.0 3,354,830 - +0.8 3,354,830 - +0.3 3,354,830 - +0.3 3,354,864 + -1.1 3,354,865 - -2.9 3,354,865 - +0.9 3,354,867 + -0.1 3,354,867 + +1.0 3,354,924 + -0.7 3,354,925 - +0.7
Or see this region's nucleotide sequence