Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2752 and Pf6N2E2_2753 are separated by 63 nucleotides Pf6N2E2_2753 and Pf6N2E2_2754 are separated by 234 nucleotides
Pf6N2E2_2752 - Exported zinc metalloprotease YfgC precursor, at 3,243,493 to 3,244,926
_2752
Pf6N2E2_2753 - HtrA protease/chaperone protein, at 3,244,990 to 3,246,420
_2753
Pf6N2E2_2754 - Sigma factor RpoE negative regulatory protein RseB precursor, at 3,246,655 to 3,247,602
_2754
Position (kb)
3244
3245
3246
3247 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3244.117 kb on + strand, within Pf6N2E2_2752 at 3244.321 kb on + strand, within Pf6N2E2_2752 at 3244.321 kb on + strand, within Pf6N2E2_2752 at 3244.321 kb on + strand, within Pf6N2E2_2752 at 3244.322 kb on - strand, within Pf6N2E2_2752 at 3244.322 kb on - strand, within Pf6N2E2_2752 at 3244.399 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.495 kb on + strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.496 kb on - strand, within Pf6N2E2_2752 at 3244.579 kb on + strand, within Pf6N2E2_2752 at 3244.592 kb on - strand, within Pf6N2E2_2752 at 3244.705 kb on + strand, within Pf6N2E2_2752 at 3244.705 kb on + strand, within Pf6N2E2_2752 at 3244.705 kb on + strand, within Pf6N2E2_2752 at 3244.771 kb on + strand, within Pf6N2E2_2752 at 3244.943 kb on - strand at 3244.943 kb on - strand at 3245.130 kb on + strand at 3245.131 kb on - strand at 3245.131 kb on - strand at 3245.410 kb on + strand, within Pf6N2E2_2753 at 3245.410 kb on + strand, within Pf6N2E2_2753 at 3245.410 kb on + strand, within Pf6N2E2_2753 at 3245.709 kb on - strand, within Pf6N2E2_2753 at 3245.811 kb on - strand, within Pf6N2E2_2753 at 3246.044 kb on - strand, within Pf6N2E2_2753 at 3246.093 kb on - strand, within Pf6N2E2_2753 at 3246.109 kb on - strand, within Pf6N2E2_2753 at 3246.538 kb on - strand at 3246.873 kb on - strand, within Pf6N2E2_2754 at 3246.873 kb on - strand, within Pf6N2E2_2754 at 3247.303 kb on - strand, within Pf6N2E2_2754 at 3247.344 kb on + strand, within Pf6N2E2_2754 at 3247.344 kb on + strand, within Pf6N2E2_2754
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 3,244,117 + Pf6N2E2_2752 0.44 +0.6 3,244,321 + Pf6N2E2_2752 0.58 +1.6 3,244,321 + Pf6N2E2_2752 0.58 -0.0 3,244,321 + Pf6N2E2_2752 0.58 +0.8 3,244,322 - Pf6N2E2_2752 0.58 +1.7 3,244,322 - Pf6N2E2_2752 0.58 +2.0 3,244,399 + Pf6N2E2_2752 0.63 +0.2 3,244,495 + Pf6N2E2_2752 0.70 +0.5 3,244,495 + Pf6N2E2_2752 0.70 +1.3 3,244,495 + Pf6N2E2_2752 0.70 +0.3 3,244,495 + Pf6N2E2_2752 0.70 +0.5 3,244,495 + Pf6N2E2_2752 0.70 +1.3 3,244,495 + Pf6N2E2_2752 0.70 -0.0 3,244,495 + Pf6N2E2_2752 0.70 -0.6 3,244,496 - Pf6N2E2_2752 0.70 +2.5 3,244,496 - Pf6N2E2_2752 0.70 +1.8 3,244,496 - Pf6N2E2_2752 0.70 +0.2 3,244,496 - Pf6N2E2_2752 0.70 +2.1 3,244,496 - Pf6N2E2_2752 0.70 +1.0 3,244,496 - Pf6N2E2_2752 0.70 -0.9 3,244,496 - Pf6N2E2_2752 0.70 +0.9 3,244,496 - Pf6N2E2_2752 0.70 +0.2 3,244,496 - Pf6N2E2_2752 0.70 +2.0 3,244,496 - Pf6N2E2_2752 0.70 +2.0 3,244,496 - Pf6N2E2_2752 0.70 +0.8 3,244,579 + Pf6N2E2_2752 0.76 +0.6 3,244,592 - Pf6N2E2_2752 0.77 -1.0 3,244,705 + Pf6N2E2_2752 0.85 +1.7 3,244,705 + Pf6N2E2_2752 0.85 +0.8 3,244,705 + Pf6N2E2_2752 0.85 -3.2 3,244,771 + Pf6N2E2_2752 0.89 -0.8 3,244,943 - +0.1 3,244,943 - +1.4 3,245,130 + -1.2 3,245,131 - +0.7 3,245,131 - +2.8 3,245,410 + Pf6N2E2_2753 0.29 -2.4 3,245,410 + Pf6N2E2_2753 0.29 -2.0 3,245,410 + Pf6N2E2_2753 0.29 -1.8 3,245,709 - Pf6N2E2_2753 0.50 -0.6 3,245,811 - Pf6N2E2_2753 0.57 -2.0 3,246,044 - Pf6N2E2_2753 0.74 -0.4 3,246,093 - Pf6N2E2_2753 0.77 -2.2 3,246,109 - Pf6N2E2_2753 0.78 -1.6 3,246,538 - -0.0 3,246,873 - Pf6N2E2_2754 0.23 -0.4 3,246,873 - Pf6N2E2_2754 0.23 -1.5 3,247,303 - Pf6N2E2_2754 0.68 +1.0 3,247,344 + Pf6N2E2_2754 0.73 +1.0 3,247,344 + Pf6N2E2_2754 0.73 -0.2
Or see this region's nucleotide sequence