Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2533 and Pf6N2E2_2534 are separated by 268 nucleotides Pf6N2E2_2534 and Pf6N2E2_2535 are separated by 12 nucleotides Pf6N2E2_2535 and Pf6N2E2_2536 are separated by 202 nucleotides
Pf6N2E2_2533 - Rhodanese domain protein UPF0176, at 2,992,076 to 2,993,017
_2533
Pf6N2E2_2534 - Cell division protein BolA, at 2,993,286 to 2,993,585
_2534
Pf6N2E2_2535 - FIG00954136: hypothetical protein, at 2,993,598 to 2,994,116
_2535
Pf6N2E2_2536 - Fumarate hydratase class II (EC 4.2.1.2), at 2,994,319 to 2,995,713
_2536
Position (kb)
2993
2994 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2992.294 kb on - strand, within Pf6N2E2_2533 at 2992.294 kb on - strand, within Pf6N2E2_2533 at 2992.294 kb on - strand, within Pf6N2E2_2533 at 2992.419 kb on + strand, within Pf6N2E2_2533 at 2992.419 kb on + strand, within Pf6N2E2_2533 at 2992.420 kb on - strand, within Pf6N2E2_2533 at 2992.420 kb on - strand, within Pf6N2E2_2533 at 2992.420 kb on - strand, within Pf6N2E2_2533 at 2992.449 kb on + strand, within Pf6N2E2_2533 at 2992.449 kb on + strand, within Pf6N2E2_2533 at 2992.449 kb on + strand, within Pf6N2E2_2533 at 2992.450 kb on - strand, within Pf6N2E2_2533 at 2992.450 kb on - strand, within Pf6N2E2_2533 at 2992.450 kb on - strand, within Pf6N2E2_2533 at 2992.450 kb on - strand, within Pf6N2E2_2533 at 2992.450 kb on - strand, within Pf6N2E2_2533 at 2992.450 kb on - strand, within Pf6N2E2_2533 at 2992.537 kb on - strand, within Pf6N2E2_2533 at 2992.680 kb on + strand, within Pf6N2E2_2533 at 2992.680 kb on + strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.700 kb on - strand, within Pf6N2E2_2533 at 2992.761 kb on + strand, within Pf6N2E2_2533 at 2992.762 kb on - strand, within Pf6N2E2_2533 at 2992.782 kb on + strand, within Pf6N2E2_2533 at 2992.962 kb on + strand at 2992.963 kb on - strand at 2992.984 kb on + strand at 2992.984 kb on + strand at 2992.984 kb on + strand at 2992.984 kb on + strand at 2992.985 kb on - strand at 2992.985 kb on - strand at 2992.985 kb on - strand at 2992.985 kb on - strand at 2992.985 kb on - strand at 2992.986 kb on + strand at 2992.986 kb on + strand at 2992.986 kb on + strand at 2992.986 kb on + strand at 2992.986 kb on + strand at 2992.987 kb on - strand at 2993.046 kb on - strand at 2993.046 kb on - strand at 2993.046 kb on - strand at 2993.103 kb on + strand at 2993.124 kb on - strand at 2993.124 kb on - strand at 2993.124 kb on - strand at 2993.124 kb on - strand at 2993.359 kb on + strand, within Pf6N2E2_2534 at 2993.360 kb on - strand, within Pf6N2E2_2534 at 2993.360 kb on - strand, within Pf6N2E2_2534 at 2993.413 kb on + strand, within Pf6N2E2_2534 at 2993.413 kb on + strand, within Pf6N2E2_2534 at 2993.414 kb on - strand, within Pf6N2E2_2534 at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.586 kb on - strand at 2993.586 kb on - strand at 2993.770 kb on + strand, within Pf6N2E2_2535 at 2993.771 kb on - strand, within Pf6N2E2_2535 at 2993.771 kb on - strand, within Pf6N2E2_2535 at 2993.818 kb on + strand, within Pf6N2E2_2535 at 2993.819 kb on - strand, within Pf6N2E2_2535 at 2993.819 kb on - strand, within Pf6N2E2_2535 at 2993.894 kb on - strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2994.132 kb on + strand at 2994.133 kb on - strand at 2994.284 kb on - strand at 2994.284 kb on - strand at 2994.303 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 2,992,294 - Pf6N2E2_2533 0.23 +1.1 2,992,294 - Pf6N2E2_2533 0.23 -2.5 2,992,294 - Pf6N2E2_2533 0.23 -1.2 2,992,419 + Pf6N2E2_2533 0.36 -1.0 2,992,419 + Pf6N2E2_2533 0.36 +1.2 2,992,420 - Pf6N2E2_2533 0.37 -0.4 2,992,420 - Pf6N2E2_2533 0.37 -0.6 2,992,420 - Pf6N2E2_2533 0.37 +0.1 2,992,449 + Pf6N2E2_2533 0.40 -0.2 2,992,449 + Pf6N2E2_2533 0.40 +0.7 2,992,449 + Pf6N2E2_2533 0.40 -1.2 2,992,450 - Pf6N2E2_2533 0.40 +1.8 2,992,450 - Pf6N2E2_2533 0.40 -1.0 2,992,450 - Pf6N2E2_2533 0.40 +0.2 2,992,450 - Pf6N2E2_2533 0.40 +0.7 2,992,450 - Pf6N2E2_2533 0.40 -1.0 2,992,450 - Pf6N2E2_2533 0.40 +0.9 2,992,537 - Pf6N2E2_2533 0.49 -0.8 2,992,680 + Pf6N2E2_2533 0.64 -1.7 2,992,680 + Pf6N2E2_2533 0.64 +0.4 2,992,681 - Pf6N2E2_2533 0.64 +0.3 2,992,681 - Pf6N2E2_2533 0.64 +1.6 2,992,681 - Pf6N2E2_2533 0.64 -1.9 2,992,681 - Pf6N2E2_2533 0.64 -0.2 2,992,681 - Pf6N2E2_2533 0.64 -1.0 2,992,681 - Pf6N2E2_2533 0.64 -0.9 2,992,681 - Pf6N2E2_2533 0.64 -1.2 2,992,681 - Pf6N2E2_2533 0.64 -1.0 2,992,681 - Pf6N2E2_2533 0.64 +0.3 2,992,681 - Pf6N2E2_2533 0.64 -3.3 2,992,700 - Pf6N2E2_2533 0.66 -1.2 2,992,761 + Pf6N2E2_2533 0.73 +1.2 2,992,762 - Pf6N2E2_2533 0.73 +0.2 2,992,782 + Pf6N2E2_2533 0.75 -1.3 2,992,962 + +0.2 2,992,963 - +1.6 2,992,984 + +0.1 2,992,984 + +1.0 2,992,984 + +0.8 2,992,984 + +0.6 2,992,985 - -1.3 2,992,985 - +0.3 2,992,985 - +0.0 2,992,985 - -0.3 2,992,985 - -0.6 2,992,986 + -0.7 2,992,986 + -0.3 2,992,986 + +0.1 2,992,986 + -0.4 2,992,986 + -0.2 2,992,987 - -1.8 2,993,046 - +0.3 2,993,046 - +0.3 2,993,046 - +0.1 2,993,103 + -0.2 2,993,124 - -0.2 2,993,124 - +0.2 2,993,124 - +0.0 2,993,124 - -0.2 2,993,359 + Pf6N2E2_2534 0.24 +0.3 2,993,360 - Pf6N2E2_2534 0.25 -1.2 2,993,360 - Pf6N2E2_2534 0.25 -1.4 2,993,413 + Pf6N2E2_2534 0.42 -3.9 2,993,413 + Pf6N2E2_2534 0.42 -0.5 2,993,414 - Pf6N2E2_2534 0.43 -2.7 2,993,585 + +0.1 2,993,585 + -1.2 2,993,585 + +1.4 2,993,585 + -2.7 2,993,585 + -0.2 2,993,585 + +1.2 2,993,586 - +0.3 2,993,586 - -0.4 2,993,770 + Pf6N2E2_2535 0.33 -1.7 2,993,771 - Pf6N2E2_2535 0.33 -0.3 2,993,771 - Pf6N2E2_2535 0.33 +1.8 2,993,818 + Pf6N2E2_2535 0.42 -0.9 2,993,819 - Pf6N2E2_2535 0.43 -0.9 2,993,819 - Pf6N2E2_2535 0.43 +0.4 2,993,894 - Pf6N2E2_2535 0.57 -2.8 2,993,974 + Pf6N2E2_2535 0.72 +0.4 2,993,974 + Pf6N2E2_2535 0.72 -3.2 2,993,974 + Pf6N2E2_2535 0.72 -1.2 2,993,974 + Pf6N2E2_2535 0.72 -0.6 2,993,974 + Pf6N2E2_2535 0.72 -0.4 2,993,975 - Pf6N2E2_2535 0.73 -1.4 2,993,975 - Pf6N2E2_2535 0.73 -0.2 2,993,975 - Pf6N2E2_2535 0.73 -1.2 2,993,975 - Pf6N2E2_2535 0.73 -0.3 2,993,975 - Pf6N2E2_2535 0.73 +1.0 2,993,975 - Pf6N2E2_2535 0.73 -1.2 2,993,975 - Pf6N2E2_2535 0.73 -0.5 2,994,132 + -0.7 2,994,133 - +0.1 2,994,284 - +0.2 2,994,284 - +0.1 2,994,303 + -0.5
Or see this region's nucleotide sequence