Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_241

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_240 and Pf6N2E2_241 are separated by 482 nucleotidesPf6N2E2_241 and Pf6N2E2_242 overlap by 1 nucleotidesPf6N2E2_242 and Pf6N2E2_243 are separated by 49 nucleotides Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893 _240 Pf6N2E2_241 - hypothetical protein, at 302,376 to 302,519 _241 Pf6N2E2_242 - Glycine betaine ABC transport system permease protein, at 302,519 to 303,202 _242 Pf6N2E2_243 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 303,252 to 304,157 _243 Position (kb) 302 303Strain fitness (log2 ratio) -2 -1 0 1at 301.423 kb on - strand, within Pf6N2E2_240at 301.641 kb on + strand, within Pf6N2E2_240at 301.642 kb on - strand, within Pf6N2E2_240at 301.952 kb on + strandat 301.981 kb on + strandat 302.115 kb on - strandat 302.157 kb on + strandat 302.157 kb on + strandat 302.157 kb on + strandat 302.244 kb on + strandat 302.336 kb on - strandat 302.481 kb on + strand, within Pf6N2E2_241at 302.540 kb on + strandat 302.540 kb on + strandat 302.612 kb on + strand, within Pf6N2E2_242at 302.677 kb on + strand, within Pf6N2E2_242at 302.682 kb on - strand, within Pf6N2E2_242at 302.840 kb on - strand, within Pf6N2E2_242at 302.843 kb on + strand, within Pf6N2E2_242at 302.843 kb on + strand, within Pf6N2E2_242at 302.843 kb on + strand, within Pf6N2E2_242at 302.843 kb on + strand, within Pf6N2E2_242at 302.843 kb on + strand, within Pf6N2E2_242at 302.843 kb on + strand, within Pf6N2E2_242at 302.843 kb on + strand, within Pf6N2E2_242at 302.843 kb on + strand, within Pf6N2E2_242at 302.844 kb on - strand, within Pf6N2E2_242at 302.844 kb on - strand, within Pf6N2E2_242at 302.989 kb on + strand, within Pf6N2E2_242at 302.989 kb on + strand, within Pf6N2E2_242at 302.989 kb on + strand, within Pf6N2E2_242at 302.990 kb on - strand, within Pf6N2E2_242at 303.196 kb on + strandat 303.405 kb on + strand, within Pf6N2E2_243at 303.405 kb on + strand, within Pf6N2E2_243at 303.405 kb on + strand, within Pf6N2E2_243at 303.405 kb on + strand, within Pf6N2E2_243at 303.406 kb on - strand, within Pf6N2E2_243at 303.519 kb on + strand, within Pf6N2E2_243

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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301,423 - Pf6N2E2_240 0.47 -1.5
301,641 + Pf6N2E2_240 0.72 -0.2
301,642 - Pf6N2E2_240 0.72 +1.0
301,952 + +0.8
301,981 + +1.2
302,115 - -1.8
302,157 + +0.3
302,157 + -1.2
302,157 + +0.4
302,244 + -1.2
302,336 - +0.8
302,481 + Pf6N2E2_241 0.73 -0.4
302,540 + +0.2
302,540 + +1.7
302,612 + Pf6N2E2_242 0.14 -0.2
302,677 + Pf6N2E2_242 0.23 +0.5
302,682 - Pf6N2E2_242 0.24 +1.4
302,840 - Pf6N2E2_242 0.47 +0.3
302,843 + Pf6N2E2_242 0.47 -0.8
302,843 + Pf6N2E2_242 0.47 +0.2
302,843 + Pf6N2E2_242 0.47 -0.4
302,843 + Pf6N2E2_242 0.47 -0.8
302,843 + Pf6N2E2_242 0.47 -1.1
302,843 + Pf6N2E2_242 0.47 -0.3
302,843 + Pf6N2E2_242 0.47 -1.0
302,843 + Pf6N2E2_242 0.47 -0.0
302,844 - Pf6N2E2_242 0.48 -0.2
302,844 - Pf6N2E2_242 0.48 +0.6
302,989 + Pf6N2E2_242 0.69 -0.2
302,989 + Pf6N2E2_242 0.69 -0.3
302,989 + Pf6N2E2_242 0.69 +0.9
302,990 - Pf6N2E2_242 0.69 -0.5
303,196 + -1.4
303,405 + Pf6N2E2_243 0.17 +0.1
303,405 + Pf6N2E2_243 0.17 +0.9
303,405 + Pf6N2E2_243 0.17 -0.4
303,405 + Pf6N2E2_243 0.17 +1.1
303,406 - Pf6N2E2_243 0.17 +0.6
303,519 + Pf6N2E2_243 0.29 -0.0

Or see this region's nucleotide sequence