Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2257 and Pf6N2E2_2258 are separated by 178 nucleotides Pf6N2E2_2258 and Pf6N2E2_2259 are separated by 195 nucleotides Pf6N2E2_2259 and Pf6N2E2_2260 overlap by 1 nucleotides
Pf6N2E2_2257 - tRNA dihydrouridine synthase A, at 2,672,582 to 2,673,520
_2257
Pf6N2E2_2258 - Transaldolase (EC 2.2.1.2), at 2,673,699 to 2,674,625
_2258
Pf6N2E2_2259 - Serine-protein kinase RsbW (EC 2.7.11.1), at 2,674,821 to 2,675,303
_2259
Pf6N2E2_2260 - Serine phosphatase RsbU, regulator of sigma subunit, at 2,675,303 to 2,676,484
_2260
Position (kb)
2673
2674
2675 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2672.854 kb on + strand, within Pf6N2E2_2257 at 2672.854 kb on + strand, within Pf6N2E2_2257 at 2672.854 kb on + strand, within Pf6N2E2_2257 at 2672.854 kb on + strand, within Pf6N2E2_2257 at 2672.854 kb on + strand, within Pf6N2E2_2257 at 2672.854 kb on + strand, within Pf6N2E2_2257 at 2672.855 kb on - strand, within Pf6N2E2_2257 at 2672.855 kb on - strand, within Pf6N2E2_2257 at 2672.855 kb on - strand, within Pf6N2E2_2257 at 2672.978 kb on + strand, within Pf6N2E2_2257 at 2672.979 kb on - strand, within Pf6N2E2_2257 at 2673.108 kb on - strand, within Pf6N2E2_2257 at 2673.239 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.338 kb on + strand, within Pf6N2E2_2257 at 2673.339 kb on - strand, within Pf6N2E2_2257 at 2673.339 kb on - strand, within Pf6N2E2_2257 at 2673.339 kb on - strand, within Pf6N2E2_2257 at 2673.341 kb on - strand, within Pf6N2E2_2257 at 2673.341 kb on - strand, within Pf6N2E2_2257 at 2673.560 kb on + strand at 2673.560 kb on + strand at 2673.561 kb on - strand at 2673.561 kb on - strand at 2673.954 kb on - strand, within Pf6N2E2_2258 at 2674.094 kb on + strand, within Pf6N2E2_2258 at 2674.095 kb on - strand, within Pf6N2E2_2258 at 2674.220 kb on + strand, within Pf6N2E2_2258 at 2674.220 kb on + strand, within Pf6N2E2_2258 at 2674.224 kb on + strand at 2674.224 kb on + strand, within Pf6N2E2_2258 at 2674.224 kb on + strand, within Pf6N2E2_2258 at 2674.225 kb on - strand, within Pf6N2E2_2258 at 2674.225 kb on - strand, within Pf6N2E2_2258 at 2674.233 kb on + strand, within Pf6N2E2_2258 at 2674.233 kb on + strand, within Pf6N2E2_2258 at 2674.233 kb on + strand, within Pf6N2E2_2258 at 2674.234 kb on - strand, within Pf6N2E2_2258 at 2674.234 kb on - strand, within Pf6N2E2_2258 at 2674.258 kb on - strand, within Pf6N2E2_2258 at 2674.258 kb on - strand, within Pf6N2E2_2258 at 2674.258 kb on - strand, within Pf6N2E2_2258 at 2674.302 kb on + strand, within Pf6N2E2_2258 at 2674.302 kb on + strand, within Pf6N2E2_2258 at 2674.302 kb on + strand, within Pf6N2E2_2258 at 2674.302 kb on + strand, within Pf6N2E2_2258 at 2674.302 kb on + strand, within Pf6N2E2_2258 at 2674.302 kb on + strand, within Pf6N2E2_2258 at 2674.303 kb on - strand, within Pf6N2E2_2258 at 2674.308 kb on + strand, within Pf6N2E2_2258 at 2674.308 kb on + strand, within Pf6N2E2_2258 at 2674.309 kb on - strand, within Pf6N2E2_2258 at 2674.309 kb on - strand, within Pf6N2E2_2258 at 2674.309 kb on - strand, within Pf6N2E2_2258 at 2674.309 kb on - strand, within Pf6N2E2_2258 at 2674.311 kb on + strand, within Pf6N2E2_2258 at 2674.312 kb on - strand, within Pf6N2E2_2258 at 2674.312 kb on - strand, within Pf6N2E2_2258 at 2674.327 kb on - strand, within Pf6N2E2_2258 at 2674.327 kb on - strand, within Pf6N2E2_2258 at 2674.565 kb on + strand at 2674.565 kb on + strand at 2674.565 kb on + strand at 2674.566 kb on - strand at 2674.566 kb on - strand at 2674.680 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 2,672,854 + Pf6N2E2_2257 0.29 +1.1 2,672,854 + Pf6N2E2_2257 0.29 -0.5 2,672,854 + Pf6N2E2_2257 0.29 -0.0 2,672,854 + Pf6N2E2_2257 0.29 -0.5 2,672,854 + Pf6N2E2_2257 0.29 +0.5 2,672,854 + Pf6N2E2_2257 0.29 +1.2 2,672,855 - Pf6N2E2_2257 0.29 +0.4 2,672,855 - Pf6N2E2_2257 0.29 -0.8 2,672,855 - Pf6N2E2_2257 0.29 -0.5 2,672,978 + Pf6N2E2_2257 0.42 -0.0 2,672,979 - Pf6N2E2_2257 0.42 +0.9 2,673,108 - Pf6N2E2_2257 0.56 +0.0 2,673,239 + Pf6N2E2_2257 0.70 -0.2 2,673,338 + Pf6N2E2_2257 0.81 +0.9 2,673,338 + Pf6N2E2_2257 0.81 +0.9 2,673,338 + Pf6N2E2_2257 0.81 +0.0 2,673,338 + Pf6N2E2_2257 0.81 -0.3 2,673,338 + Pf6N2E2_2257 0.81 +1.7 2,673,338 + Pf6N2E2_2257 0.81 -1.5 2,673,338 + Pf6N2E2_2257 0.81 -0.7 2,673,338 + Pf6N2E2_2257 0.81 +0.1 2,673,338 + Pf6N2E2_2257 0.81 +2.0 2,673,339 - Pf6N2E2_2257 0.81 +0.9 2,673,339 - Pf6N2E2_2257 0.81 -0.7 2,673,339 - Pf6N2E2_2257 0.81 +0.0 2,673,341 - Pf6N2E2_2257 0.81 -0.4 2,673,341 - Pf6N2E2_2257 0.81 +1.0 2,673,560 + +0.3 2,673,560 + +1.0 2,673,561 - +0.7 2,673,561 - -0.2 2,673,954 - Pf6N2E2_2258 0.28 -2.8 2,674,094 + Pf6N2E2_2258 0.43 -3.4 2,674,095 - Pf6N2E2_2258 0.43 -0.8 2,674,220 + Pf6N2E2_2258 0.56 -2.1 2,674,220 + Pf6N2E2_2258 0.56 -1.8 2,674,224 + -1.2 2,674,224 + Pf6N2E2_2258 0.57 -2.2 2,674,224 + Pf6N2E2_2258 0.57 -2.0 2,674,225 - Pf6N2E2_2258 0.57 -3.5 2,674,225 - Pf6N2E2_2258 0.57 -1.8 2,674,233 + Pf6N2E2_2258 0.58 -1.2 2,674,233 + Pf6N2E2_2258 0.58 -2.1 2,674,233 + Pf6N2E2_2258 0.58 -2.4 2,674,234 - Pf6N2E2_2258 0.58 -2.2 2,674,234 - Pf6N2E2_2258 0.58 -2.5 2,674,258 - Pf6N2E2_2258 0.60 -1.9 2,674,258 - Pf6N2E2_2258 0.60 -2.5 2,674,258 - Pf6N2E2_2258 0.60 -2.8 2,674,302 + Pf6N2E2_2258 0.65 -2.4 2,674,302 + Pf6N2E2_2258 0.65 -1.2 2,674,302 + Pf6N2E2_2258 0.65 -1.8 2,674,302 + Pf6N2E2_2258 0.65 -1.2 2,674,302 + Pf6N2E2_2258 0.65 -0.9 2,674,302 + Pf6N2E2_2258 0.65 -2.4 2,674,303 - Pf6N2E2_2258 0.65 -3.4 2,674,308 + Pf6N2E2_2258 0.66 -2.9 2,674,308 + Pf6N2E2_2258 0.66 -4.7 2,674,309 - Pf6N2E2_2258 0.66 -2.4 2,674,309 - Pf6N2E2_2258 0.66 -0.7 2,674,309 - Pf6N2E2_2258 0.66 -0.2 2,674,309 - Pf6N2E2_2258 0.66 -1.4 2,674,311 + Pf6N2E2_2258 0.66 -2.2 2,674,312 - Pf6N2E2_2258 0.66 -1.2 2,674,312 - Pf6N2E2_2258 0.66 -2.2 2,674,327 - Pf6N2E2_2258 0.68 -1.7 2,674,327 - Pf6N2E2_2258 0.68 -0.9 2,674,565 + -3.4 2,674,565 + -3.5 2,674,565 + -3.4 2,674,566 - -2.0 2,674,566 - -0.9 2,674,680 - -1.1
Or see this region's nucleotide sequence