Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2127 and Pf6N2E2_2128 are separated by 109 nucleotides Pf6N2E2_2128 and Pf6N2E2_2129 are separated by 79 nucleotides
Pf6N2E2_2127 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 2,531,285 to 2,532,694
_2127
Pf6N2E2_2128 - Short-chain dehydrogenase/reductase SDR, at 2,532,804 to 2,533,550
_2128
Pf6N2E2_2129 - Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup, at 2,533,630 to 2,534,508
_2129
Position (kb)
2532
2533
2534 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2531.894 kb on + strand, within Pf6N2E2_2127 at 2531.894 kb on + strand, within Pf6N2E2_2127 at 2531.895 kb on - strand, within Pf6N2E2_2127 at 2531.895 kb on - strand, within Pf6N2E2_2127 at 2532.146 kb on + strand, within Pf6N2E2_2127 at 2532.147 kb on - strand, within Pf6N2E2_2127 at 2532.350 kb on + strand, within Pf6N2E2_2127 at 2532.350 kb on + strand, within Pf6N2E2_2127 at 2532.350 kb on + strand, within Pf6N2E2_2127 at 2532.443 kb on + strand, within Pf6N2E2_2127 at 2532.486 kb on - strand, within Pf6N2E2_2127 at 2532.623 kb on + strand at 2532.790 kb on + strand at 2532.790 kb on + strand at 2532.791 kb on - strand at 2532.791 kb on - strand at 2532.845 kb on - strand at 2532.979 kb on + strand, within Pf6N2E2_2128 at 2532.979 kb on + strand, within Pf6N2E2_2128 at 2532.979 kb on + strand, within Pf6N2E2_2128 at 2533.090 kb on + strand, within Pf6N2E2_2128 at 2533.090 kb on + strand, within Pf6N2E2_2128 at 2533.090 kb on + strand, within Pf6N2E2_2128 at 2533.091 kb on - strand, within Pf6N2E2_2128 at 2533.212 kb on + strand, within Pf6N2E2_2128 at 2533.212 kb on + strand, within Pf6N2E2_2128 at 2533.213 kb on - strand, within Pf6N2E2_2128 at 2533.453 kb on + strand, within Pf6N2E2_2128 at 2533.454 kb on - strand, within Pf6N2E2_2128 at 2533.454 kb on - strand, within Pf6N2E2_2128 at 2533.545 kb on - strand at 2533.594 kb on + strand at 2533.595 kb on - strand at 2533.685 kb on + strand at 2533.685 kb on + strand at 2533.685 kb on + strand at 2533.686 kb on - strand at 2534.143 kb on - strand, within Pf6N2E2_2129 at 2534.143 kb on - strand, within Pf6N2E2_2129 at 2534.143 kb on - strand, within Pf6N2E2_2129 at 2534.167 kb on + strand, within Pf6N2E2_2129 at 2534.407 kb on + strand, within Pf6N2E2_2129 at 2534.499 kb on - strand at 2534.499 kb on - strand at 2534.501 kb on + strand at 2534.501 kb on + strand at 2534.501 kb on + strand at 2534.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 2,531,894 + Pf6N2E2_2127 0.43 -0.0 2,531,894 + Pf6N2E2_2127 0.43 -0.8 2,531,895 - Pf6N2E2_2127 0.43 +0.0 2,531,895 - Pf6N2E2_2127 0.43 +0.3 2,532,146 + Pf6N2E2_2127 0.61 +1.3 2,532,147 - Pf6N2E2_2127 0.61 -0.1 2,532,350 + Pf6N2E2_2127 0.76 -0.6 2,532,350 + Pf6N2E2_2127 0.76 -1.3 2,532,350 + Pf6N2E2_2127 0.76 -0.5 2,532,443 + Pf6N2E2_2127 0.82 -0.5 2,532,486 - Pf6N2E2_2127 0.85 -0.6 2,532,623 + +0.2 2,532,790 + -0.3 2,532,790 + +0.2 2,532,791 - -0.4 2,532,791 - +0.6 2,532,845 - -0.3 2,532,979 + Pf6N2E2_2128 0.23 -0.3 2,532,979 + Pf6N2E2_2128 0.23 -1.2 2,532,979 + Pf6N2E2_2128 0.23 +0.5 2,533,090 + Pf6N2E2_2128 0.38 -1.5 2,533,090 + Pf6N2E2_2128 0.38 -3.8 2,533,090 + Pf6N2E2_2128 0.38 -2.7 2,533,091 - Pf6N2E2_2128 0.38 +0.3 2,533,212 + Pf6N2E2_2128 0.55 -0.3 2,533,212 + Pf6N2E2_2128 0.55 -2.0 2,533,213 - Pf6N2E2_2128 0.55 -0.4 2,533,453 + Pf6N2E2_2128 0.87 -3.1 2,533,454 - Pf6N2E2_2128 0.87 -3.1 2,533,454 - Pf6N2E2_2128 0.87 -3.2 2,533,545 - -1.3 2,533,594 + -3.3 2,533,595 - -4.9 2,533,685 + -3.7 2,533,685 + -4.3 2,533,685 + -3.6 2,533,686 - -2.8 2,534,143 - Pf6N2E2_2129 0.58 -3.3 2,534,143 - Pf6N2E2_2129 0.58 -5.3 2,534,143 - Pf6N2E2_2129 0.58 -4.7 2,534,167 + Pf6N2E2_2129 0.61 -3.0 2,534,407 + Pf6N2E2_2129 0.88 -2.3 2,534,499 - -2.8 2,534,499 - -3.4 2,534,501 + -1.8 2,534,501 + -2.3 2,534,501 + -1.7 2,534,502 - -2.8
Or see this region's nucleotide sequence