Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2084 and Pf6N2E2_2085 are separated by 323 nucleotides Pf6N2E2_2085 and Pf6N2E2_2086 overlap by 4 nucleotides
Pf6N2E2_2084 - sensory box histidine kinase/response regulator, at 2,476,278 to 2,478,341
_2084
Pf6N2E2_2085 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 2,478,665 to 2,481,742
_2085
Pf6N2E2_2086 - DNA polymerase-like protein PA0670, at 2,481,739 to 2,483,160
_2086
Position (kb)
2478
2479
2480
2481
2482 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2477.752 kb on + strand, within Pf6N2E2_2084 at 2477.752 kb on + strand, within Pf6N2E2_2084 at 2477.753 kb on - strand, within Pf6N2E2_2084 at 2477.986 kb on + strand, within Pf6N2E2_2084 at 2477.986 kb on + strand, within Pf6N2E2_2084 at 2477.987 kb on - strand, within Pf6N2E2_2084 at 2477.987 kb on - strand, within Pf6N2E2_2084 at 2477.987 kb on - strand, within Pf6N2E2_2084 at 2477.987 kb on - strand, within Pf6N2E2_2084 at 2477.989 kb on + strand, within Pf6N2E2_2084 at 2477.989 kb on + strand, within Pf6N2E2_2084 at 2477.989 kb on + strand, within Pf6N2E2_2084 at 2477.989 kb on + strand, within Pf6N2E2_2084 at 2477.989 kb on + strand, within Pf6N2E2_2084 at 2477.990 kb on - strand, within Pf6N2E2_2084 at 2477.990 kb on - strand, within Pf6N2E2_2084 at 2477.990 kb on - strand, within Pf6N2E2_2084 at 2477.990 kb on - strand, within Pf6N2E2_2084 at 2478.148 kb on + strand at 2478.178 kb on + strand at 2478.179 kb on - strand at 2478.190 kb on + strand at 2478.190 kb on + strand at 2478.190 kb on + strand at 2478.190 kb on + strand at 2478.378 kb on + strand at 2478.550 kb on + strand at 2478.585 kb on + strand at 2478.586 kb on - strand at 2478.586 kb on - strand at 2478.609 kb on - strand at 2479.056 kb on + strand, within Pf6N2E2_2085 at 2479.056 kb on + strand, within Pf6N2E2_2085 at 2479.084 kb on + strand, within Pf6N2E2_2085 at 2479.085 kb on - strand, within Pf6N2E2_2085 at 2479.085 kb on - strand, within Pf6N2E2_2085 at 2479.085 kb on - strand, within Pf6N2E2_2085 at 2479.485 kb on + strand, within Pf6N2E2_2085 at 2479.485 kb on + strand, within Pf6N2E2_2085 at 2479.486 kb on - strand, within Pf6N2E2_2085 at 2479.486 kb on - strand, within Pf6N2E2_2085 at 2479.563 kb on + strand, within Pf6N2E2_2085 at 2479.564 kb on - strand, within Pf6N2E2_2085 at 2479.662 kb on + strand, within Pf6N2E2_2085 at 2479.662 kb on + strand, within Pf6N2E2_2085 at 2479.773 kb on + strand, within Pf6N2E2_2085 at 2479.773 kb on + strand, within Pf6N2E2_2085 at 2479.773 kb on + strand, within Pf6N2E2_2085 at 2479.774 kb on - strand, within Pf6N2E2_2085 at 2479.869 kb on + strand at 2479.869 kb on + strand, within Pf6N2E2_2085 at 2479.870 kb on - strand, within Pf6N2E2_2085 at 2479.870 kb on - strand, within Pf6N2E2_2085 at 2479.943 kb on + strand, within Pf6N2E2_2085 at 2479.943 kb on + strand, within Pf6N2E2_2085 at 2479.943 kb on + strand, within Pf6N2E2_2085 at 2479.971 kb on + strand, within Pf6N2E2_2085 at 2479.972 kb on - strand, within Pf6N2E2_2085 at 2479.972 kb on - strand, within Pf6N2E2_2085 at 2480.068 kb on - strand, within Pf6N2E2_2085 at 2480.068 kb on - strand, within Pf6N2E2_2085 at 2480.068 kb on - strand, within Pf6N2E2_2085 at 2480.068 kb on - strand, within Pf6N2E2_2085 at 2480.068 kb on - strand, within Pf6N2E2_2085 at 2480.107 kb on - strand at 2480.571 kb on + strand, within Pf6N2E2_2085 at 2480.572 kb on - strand, within Pf6N2E2_2085 at 2480.586 kb on + strand, within Pf6N2E2_2085 at 2480.586 kb on + strand, within Pf6N2E2_2085 at 2480.587 kb on - strand, within Pf6N2E2_2085 at 2480.587 kb on - strand, within Pf6N2E2_2085 at 2480.587 kb on - strand, within Pf6N2E2_2085 at 2480.587 kb on - strand, within Pf6N2E2_2085 at 2480.587 kb on - strand, within Pf6N2E2_2085 at 2480.686 kb on + strand, within Pf6N2E2_2085 at 2480.686 kb on + strand, within Pf6N2E2_2085 at 2480.767 kb on + strand, within Pf6N2E2_2085 at 2480.768 kb on - strand, within Pf6N2E2_2085 at 2480.768 kb on - strand, within Pf6N2E2_2085 at 2480.802 kb on - strand, within Pf6N2E2_2085 at 2480.814 kb on + strand, within Pf6N2E2_2085 at 2480.814 kb on + strand, within Pf6N2E2_2085 at 2480.815 kb on - strand, within Pf6N2E2_2085 at 2481.483 kb on + strand at 2481.719 kb on + strand at 2481.719 kb on + strand at 2481.719 kb on + strand at 2481.720 kb on - strand at 2481.720 kb on - strand at 2481.729 kb on + strand at 2481.729 kb on + strand at 2481.830 kb on + strand at 2481.830 kb on + strand at 2481.830 kb on + strand at 2481.830 kb on + strand at 2481.830 kb on + strand at 2481.831 kb on - strand at 2481.831 kb on - strand at 2481.831 kb on - strand at 2481.831 kb on - strand at 2481.833 kb on + strand at 2481.833 kb on + strand at 2481.833 kb on + strand at 2481.834 kb on - strand at 2481.834 kb on - strand at 2481.834 kb on - strand at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.427 kb on + strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.428 kb on - strand, within Pf6N2E2_2086 at 2482.570 kb on + strand, within Pf6N2E2_2086 at 2482.570 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086 at 2482.703 kb on + strand, within Pf6N2E2_2086
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 2,477,752 + Pf6N2E2_2084 0.71 -0.3 2,477,752 + Pf6N2E2_2084 0.71 -0.4 2,477,753 - Pf6N2E2_2084 0.71 -1.3 2,477,986 + Pf6N2E2_2084 0.83 +0.6 2,477,986 + Pf6N2E2_2084 0.83 +0.0 2,477,987 - Pf6N2E2_2084 0.83 -0.3 2,477,987 - Pf6N2E2_2084 0.83 -0.8 2,477,987 - Pf6N2E2_2084 0.83 +0.3 2,477,987 - Pf6N2E2_2084 0.83 +0.1 2,477,989 + Pf6N2E2_2084 0.83 +0.5 2,477,989 + Pf6N2E2_2084 0.83 +1.7 2,477,989 + Pf6N2E2_2084 0.83 +0.4 2,477,989 + Pf6N2E2_2084 0.83 +1.4 2,477,989 + Pf6N2E2_2084 0.83 +0.6 2,477,990 - Pf6N2E2_2084 0.83 +0.2 2,477,990 - Pf6N2E2_2084 0.83 +0.0 2,477,990 - Pf6N2E2_2084 0.83 +0.8 2,477,990 - Pf6N2E2_2084 0.83 +0.6 2,478,148 + +0.5 2,478,178 + -2.8 2,478,179 - +0.2 2,478,190 + -1.1 2,478,190 + +0.5 2,478,190 + +0.0 2,478,190 + -0.3 2,478,378 + +0.5 2,478,550 + +0.5 2,478,585 + -0.1 2,478,586 - -0.5 2,478,586 - -0.7 2,478,609 - -0.4 2,479,056 + Pf6N2E2_2085 0.13 +0.6 2,479,056 + Pf6N2E2_2085 0.13 +0.3 2,479,084 + Pf6N2E2_2085 0.14 +0.0 2,479,085 - Pf6N2E2_2085 0.14 -0.4 2,479,085 - Pf6N2E2_2085 0.14 -0.4 2,479,085 - Pf6N2E2_2085 0.14 -0.4 2,479,485 + Pf6N2E2_2085 0.27 +0.7 2,479,485 + Pf6N2E2_2085 0.27 +0.1 2,479,486 - Pf6N2E2_2085 0.27 +1.3 2,479,486 - Pf6N2E2_2085 0.27 -0.2 2,479,563 + Pf6N2E2_2085 0.29 +0.6 2,479,564 - Pf6N2E2_2085 0.29 -2.3 2,479,662 + Pf6N2E2_2085 0.32 -0.5 2,479,662 + Pf6N2E2_2085 0.32 +1.7 2,479,773 + Pf6N2E2_2085 0.36 +0.7 2,479,773 + Pf6N2E2_2085 0.36 +0.2 2,479,773 + Pf6N2E2_2085 0.36 -0.1 2,479,774 - Pf6N2E2_2085 0.36 +1.3 2,479,869 + +0.7 2,479,869 + Pf6N2E2_2085 0.39 -0.5 2,479,870 - Pf6N2E2_2085 0.39 +1.2 2,479,870 - Pf6N2E2_2085 0.39 +0.8 2,479,943 + Pf6N2E2_2085 0.42 -1.3 2,479,943 + Pf6N2E2_2085 0.42 -1.3 2,479,943 + Pf6N2E2_2085 0.42 +0.6 2,479,971 + Pf6N2E2_2085 0.42 -1.0 2,479,972 - Pf6N2E2_2085 0.42 +1.0 2,479,972 - Pf6N2E2_2085 0.42 -2.2 2,480,068 - Pf6N2E2_2085 0.46 +0.1 2,480,068 - Pf6N2E2_2085 0.46 -2.3 2,480,068 - Pf6N2E2_2085 0.46 +0.2 2,480,068 - Pf6N2E2_2085 0.46 +0.1 2,480,068 - Pf6N2E2_2085 0.46 +0.6 2,480,107 - -0.3 2,480,571 + Pf6N2E2_2085 0.62 +0.3 2,480,572 - Pf6N2E2_2085 0.62 +0.1 2,480,586 + Pf6N2E2_2085 0.62 -0.5 2,480,586 + Pf6N2E2_2085 0.62 +0.4 2,480,587 - Pf6N2E2_2085 0.62 +0.6 2,480,587 - Pf6N2E2_2085 0.62 +1.0 2,480,587 - Pf6N2E2_2085 0.62 +0.1 2,480,587 - Pf6N2E2_2085 0.62 +0.4 2,480,587 - Pf6N2E2_2085 0.62 +0.3 2,480,686 + Pf6N2E2_2085 0.66 +0.3 2,480,686 + Pf6N2E2_2085 0.66 +0.4 2,480,767 + Pf6N2E2_2085 0.68 -1.1 2,480,768 - Pf6N2E2_2085 0.68 +0.7 2,480,768 - Pf6N2E2_2085 0.68 -0.0 2,480,802 - Pf6N2E2_2085 0.69 -0.0 2,480,814 + Pf6N2E2_2085 0.70 +0.8 2,480,814 + Pf6N2E2_2085 0.70 -0.3 2,480,815 - Pf6N2E2_2085 0.70 +0.5 2,481,483 + +0.9 2,481,719 + -0.5 2,481,719 + -0.8 2,481,719 + -1.8 2,481,720 - -0.7 2,481,720 - -1.6 2,481,729 + +1.1 2,481,729 + +0.2 2,481,830 + +0.3 2,481,830 + +0.2 2,481,830 + -0.0 2,481,830 + +0.6 2,481,830 + -0.7 2,481,831 - -0.9 2,481,831 - -0.1 2,481,831 - +0.7 2,481,831 - -0.7 2,481,833 + -0.4 2,481,833 + -0.3 2,481,833 + -1.0 2,481,834 - +0.1 2,481,834 - +0.8 2,481,834 - -0.4 2,482,427 + Pf6N2E2_2086 0.48 +0.7 2,482,427 + Pf6N2E2_2086 0.48 +0.3 2,482,427 + Pf6N2E2_2086 0.48 -0.0 2,482,427 + Pf6N2E2_2086 0.48 +0.2 2,482,427 + Pf6N2E2_2086 0.48 +0.4 2,482,427 + Pf6N2E2_2086 0.48 -0.7 2,482,428 - Pf6N2E2_2086 0.48 -1.1 2,482,428 - Pf6N2E2_2086 0.48 -1.1 2,482,428 - Pf6N2E2_2086 0.48 +0.4 2,482,428 - Pf6N2E2_2086 0.48 +0.0 2,482,428 - Pf6N2E2_2086 0.48 -0.6 2,482,570 + Pf6N2E2_2086 0.58 +0.6 2,482,570 + Pf6N2E2_2086 0.58 +0.3 2,482,703 + Pf6N2E2_2086 0.68 +0.5 2,482,703 + Pf6N2E2_2086 0.68 +0.9 2,482,703 + Pf6N2E2_2086 0.68 +0.3 2,482,703 + Pf6N2E2_2086 0.68 -0.3 2,482,703 + Pf6N2E2_2086 0.68 -0.2 2,482,703 + Pf6N2E2_2086 0.68 -0.7
Or see this region's nucleotide sequence