Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2070 and Pf6N2E2_2071 are separated by 209 nucleotides Pf6N2E2_2071 and Pf6N2E2_2072 are separated by 123 nucleotides Pf6N2E2_2072 and Pf6N2E2_2073 overlap by 48 nucleotides Pf6N2E2_2073 and Pf6N2E2_2074 overlap by 17 nucleotides Pf6N2E2_2074 and Pf6N2E2_2075 are separated by 158 nucleotides
Pf6N2E2_2070 - Periplasmic sugar-binding domain protein, at 2,464,687 to 2,465,703
_2070
Pf6N2E2_2071 - hypothetical protein, at 2,465,913 to 2,466,026
_2071
Pf6N2E2_2072 - hypothetical protein, at 2,466,150 to 2,466,275
_2072
Pf6N2E2_2073 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 2,466,228 to 2,467,976
_2073
Pf6N2E2_2074 - required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) , at 2,467,960 to 2,468,454
_2074
Pf6N2E2_2075 - Peptidase, U7 family, at 2,468,613 to 2,469,644
_2075
Position (kb)
2466
2467
2468 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.501 kb on + strand, within Pf6N2E2_2070 at 2465.502 kb on - strand, within Pf6N2E2_2070 at 2465.502 kb on - strand, within Pf6N2E2_2070 at 2465.502 kb on - strand, within Pf6N2E2_2070 at 2465.502 kb on - strand, within Pf6N2E2_2070 at 2465.502 kb on - strand, within Pf6N2E2_2070 at 2465.525 kb on + strand, within Pf6N2E2_2070 at 2465.525 kb on + strand, within Pf6N2E2_2070 at 2465.525 kb on + strand, within Pf6N2E2_2070 at 2465.526 kb on - strand, within Pf6N2E2_2070 at 2465.526 kb on - strand, within Pf6N2E2_2070 at 2465.598 kb on - strand, within Pf6N2E2_2070 at 2465.598 kb on - strand, within Pf6N2E2_2070 at 2465.654 kb on + strand at 2465.654 kb on + strand at 2465.654 kb on + strand at 2465.654 kb on + strand at 2465.654 kb on + strand at 2465.655 kb on - strand at 2465.655 kb on - strand at 2465.655 kb on - strand at 2465.655 kb on - strand at 2465.779 kb on + strand at 2465.780 kb on - strand at 2465.780 kb on - strand at 2465.780 kb on - strand at 2465.808 kb on + strand at 2465.808 kb on + strand at 2465.808 kb on + strand at 2465.809 kb on - strand at 2465.809 kb on - strand at 2465.831 kb on - strand at 2465.833 kb on + strand at 2465.834 kb on - strand at 2465.845 kb on + strand at 2465.845 kb on + strand at 2465.845 kb on + strand at 2465.846 kb on - strand at 2465.846 kb on - strand at 2465.846 kb on - strand at 2465.846 kb on - strand at 2465.847 kb on + strand at 2465.914 kb on + strand at 2465.915 kb on - strand at 2465.915 kb on - strand at 2465.915 kb on - strand at 2465.991 kb on - strand, within Pf6N2E2_2071 at 2465.991 kb on - strand, within Pf6N2E2_2071 at 2465.991 kb on - strand, within Pf6N2E2_2071 at 2466.050 kb on + strand at 2466.050 kb on + strand at 2466.051 kb on - strand at 2466.051 kb on - strand at 2466.080 kb on + strand at 2466.080 kb on + strand at 2466.080 kb on + strand at 2466.080 kb on + strand at 2466.081 kb on - strand at 2466.081 kb on - strand at 2466.121 kb on + strand at 2466.121 kb on + strand at 2466.121 kb on + strand at 2466.122 kb on - strand at 2466.122 kb on - strand at 2466.124 kb on + strand at 2466.124 kb on + strand at 2466.125 kb on - strand at 2466.125 kb on - strand at 2466.125 kb on - strand at 2466.151 kb on + strand at 2466.151 kb on + strand at 2466.158 kb on - strand at 2466.194 kb on + strand, within Pf6N2E2_2072 at 2466.194 kb on + strand, within Pf6N2E2_2072 at 2466.194 kb on + strand, within Pf6N2E2_2072 at 2466.194 kb on + strand, within Pf6N2E2_2072 at 2466.195 kb on - strand, within Pf6N2E2_2072 at 2466.195 kb on - strand, within Pf6N2E2_2072 at 2466.214 kb on - strand, within Pf6N2E2_2072 at 2466.315 kb on + strand at 2466.315 kb on + strand at 2466.315 kb on + strand at 2466.315 kb on + strand at 2466.315 kb on + strand at 2466.315 kb on + strand at 2466.315 kb on + strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.316 kb on - strand at 2466.317 kb on + strand at 2466.317 kb on + strand at 2466.317 kb on + strand at 2466.317 kb on + strand at 2466.317 kb on + strand at 2466.317 kb on + strand at 2466.317 kb on + strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.318 kb on - strand at 2466.321 kb on + strand at 2466.321 kb on + strand at 2466.321 kb on + strand at 2466.321 kb on + strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.322 kb on - strand at 2466.325 kb on + strand at 2466.325 kb on + strand at 2466.325 kb on + strand at 2466.325 kb on + strand at 2466.326 kb on - strand at 2466.326 kb on - strand at 2466.326 kb on - strand at 2466.326 kb on - strand at 2466.326 kb on - strand at 2466.328 kb on - strand at 2466.337 kb on - strand at 2466.453 kb on + strand, within Pf6N2E2_2073 at 2466.453 kb on + strand, within Pf6N2E2_2073 at 2466.454 kb on - strand, within Pf6N2E2_2073 at 2466.454 kb on - strand, within Pf6N2E2_2073 at 2466.454 kb on - strand, within Pf6N2E2_2073 at 2466.454 kb on - strand, within Pf6N2E2_2073 at 2466.454 kb on - strand at 2466.454 kb on - strand, within Pf6N2E2_2073 at 2466.454 kb on - strand, within Pf6N2E2_2073 at 2466.454 kb on - strand, within Pf6N2E2_2073 at 2466.496 kb on - strand, within Pf6N2E2_2073 at 2466.550 kb on - strand, within Pf6N2E2_2073 at 2466.594 kb on + strand, within Pf6N2E2_2073 at 2466.595 kb on - strand, within Pf6N2E2_2073 at 2466.888 kb on + strand, within Pf6N2E2_2073 at 2466.888 kb on + strand, within Pf6N2E2_2073 at 2466.889 kb on - strand, within Pf6N2E2_2073 at 2466.920 kb on - strand, within Pf6N2E2_2073 at 2466.945 kb on - strand, within Pf6N2E2_2073 at 2467.026 kb on + strand, within Pf6N2E2_2073 at 2467.026 kb on + strand, within Pf6N2E2_2073 at 2467.027 kb on - strand, within Pf6N2E2_2073 at 2467.027 kb on - strand, within Pf6N2E2_2073 at 2467.027 kb on - strand, within Pf6N2E2_2073 at 2467.209 kb on + strand, within Pf6N2E2_2073 at 2467.317 kb on + strand, within Pf6N2E2_2073 at 2467.648 kb on - strand, within Pf6N2E2_2073 at 2467.764 kb on + strand, within Pf6N2E2_2073 at 2467.764 kb on + strand, within Pf6N2E2_2073 at 2467.878 kb on + strand at 2467.878 kb on + strand at 2467.878 kb on + strand at 2467.879 kb on - strand at 2467.892 kb on + strand at 2467.892 kb on + strand at 2467.920 kb on + strand at 2467.920 kb on + strand at 2467.920 kb on + strand at 2467.921 kb on - strand at 2467.921 kb on - strand at 2467.921 kb on - strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.928 kb on + strand at 2467.959 kb on + strand at 2467.959 kb on + strand at 2467.960 kb on - strand at 2468.314 kb on + strand, within Pf6N2E2_2074 at 2468.315 kb on - strand, within Pf6N2E2_2074 at 2468.407 kb on + strand at 2468.407 kb on + strand at 2468.408 kb on - strand at 2468.603 kb on - strand at 2468.603 kb on - strand at 2468.615 kb on - strand at 2468.615 kb on - strand at 2468.723 kb on - strand, within Pf6N2E2_2075 at 2468.735 kb on - strand, within Pf6N2E2_2075 at 2468.873 kb on - strand, within Pf6N2E2_2075
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 2,465,501 + Pf6N2E2_2070 0.80 +1.3 2,465,501 + Pf6N2E2_2070 0.80 +0.8 2,465,501 + Pf6N2E2_2070 0.80 +0.3 2,465,501 + Pf6N2E2_2070 0.80 +0.5 2,465,501 + Pf6N2E2_2070 0.80 -0.5 2,465,501 + Pf6N2E2_2070 0.80 -0.7 2,465,501 + Pf6N2E2_2070 0.80 +0.5 2,465,501 + Pf6N2E2_2070 0.80 -0.6 2,465,501 + Pf6N2E2_2070 0.80 +0.6 2,465,502 - Pf6N2E2_2070 0.80 +1.0 2,465,502 - Pf6N2E2_2070 0.80 -0.3 2,465,502 - Pf6N2E2_2070 0.80 -0.5 2,465,502 - Pf6N2E2_2070 0.80 -0.3 2,465,502 - Pf6N2E2_2070 0.80 +1.6 2,465,525 + Pf6N2E2_2070 0.82 +0.7 2,465,525 + Pf6N2E2_2070 0.82 -0.5 2,465,525 + Pf6N2E2_2070 0.82 -0.7 2,465,526 - Pf6N2E2_2070 0.82 +0.4 2,465,526 - Pf6N2E2_2070 0.82 -0.6 2,465,598 - Pf6N2E2_2070 0.90 -0.5 2,465,598 - Pf6N2E2_2070 0.90 +0.4 2,465,654 + +0.4 2,465,654 + +1.5 2,465,654 + +0.1 2,465,654 + +1.1 2,465,654 + -0.5 2,465,655 - +0.7 2,465,655 - -2.0 2,465,655 - +0.8 2,465,655 - -0.5 2,465,779 + +0.3 2,465,780 - +0.2 2,465,780 - +0.9 2,465,780 - -1.1 2,465,808 + +0.1 2,465,808 + +0.6 2,465,808 + +0.8 2,465,809 - +1.1 2,465,809 - +0.6 2,465,831 - -0.8 2,465,833 + -1.3 2,465,834 - -0.1 2,465,845 + +0.3 2,465,845 + +0.7 2,465,845 + -0.3 2,465,846 - +0.0 2,465,846 - -0.5 2,465,846 - -0.3 2,465,846 - +0.1 2,465,847 + -1.7 2,465,914 + +1.5 2,465,915 - -0.5 2,465,915 - +1.7 2,465,915 - +0.3 2,465,991 - Pf6N2E2_2071 0.68 -0.2 2,465,991 - Pf6N2E2_2071 0.68 +0.0 2,465,991 - Pf6N2E2_2071 0.68 -0.0 2,466,050 + -0.3 2,466,050 + +1.0 2,466,051 - -0.3 2,466,051 - -0.3 2,466,080 + -2.3 2,466,080 + +0.6 2,466,080 + +0.6 2,466,080 + +0.2 2,466,081 - -1.3 2,466,081 - -1.2 2,466,121 + -0.3 2,466,121 + -0.9 2,466,121 + +0.2 2,466,122 - -0.7 2,466,122 - -0.0 2,466,124 + -0.3 2,466,124 + -1.6 2,466,125 - +0.3 2,466,125 - +0.0 2,466,125 - +0.1 2,466,151 + +0.2 2,466,151 + +1.2 2,466,158 - -0.1 2,466,194 + Pf6N2E2_2072 0.35 -0.3 2,466,194 + Pf6N2E2_2072 0.35 -0.1 2,466,194 + Pf6N2E2_2072 0.35 -0.9 2,466,194 + Pf6N2E2_2072 0.35 +1.0 2,466,195 - Pf6N2E2_2072 0.36 -2.1 2,466,195 - Pf6N2E2_2072 0.36 -0.9 2,466,214 - Pf6N2E2_2072 0.51 +0.6 2,466,315 + -0.3 2,466,315 + +0.5 2,466,315 + -1.0 2,466,315 + +0.3 2,466,315 + -1.0 2,466,315 + -2.6 2,466,315 + -1.3 2,466,316 - +0.4 2,466,316 - -0.8 2,466,316 - -1.3 2,466,316 - -0.7 2,466,316 - -0.3 2,466,316 - -0.5 2,466,316 - +0.6 2,466,316 - +0.7 2,466,316 - +0.4 2,466,316 - -0.8 2,466,316 - -2.8 2,466,317 + -1.0 2,466,317 + -1.8 2,466,317 + -2.0 2,466,317 + -0.3 2,466,317 + +0.5 2,466,317 + +0.7 2,466,317 + +0.9 2,466,318 - -0.7 2,466,318 - -0.4 2,466,318 - +0.7 2,466,318 - -0.6 2,466,318 - -3.3 2,466,318 - -0.9 2,466,318 - +0.3 2,466,318 - -1.1 2,466,318 - -0.5 2,466,318 - -0.1 2,466,318 - +0.7 2,466,318 - -1.8 2,466,318 - -1.1 2,466,318 - +0.5 2,466,318 - -0.8 2,466,318 - -1.1 2,466,318 - -0.6 2,466,318 - +0.7 2,466,318 - +0.2 2,466,321 + -2.8 2,466,321 + +0.3 2,466,321 + -0.7 2,466,321 + +1.3 2,466,322 - -0.3 2,466,322 - +0.4 2,466,322 - -0.1 2,466,322 - -0.8 2,466,322 - -0.4 2,466,322 - -0.5 2,466,322 - +1.1 2,466,322 - +0.3 2,466,322 - -1.3 2,466,322 - -1.0 2,466,325 + +0.4 2,466,325 + +1.3 2,466,325 + +0.4 2,466,325 + -1.5 2,466,326 - +0.6 2,466,326 - +1.1 2,466,326 - -2.3 2,466,326 - -1.4 2,466,326 - +0.3 2,466,328 - +1.1 2,466,337 - -0.3 2,466,453 + Pf6N2E2_2073 0.13 -1.2 2,466,453 + Pf6N2E2_2073 0.13 -0.7 2,466,454 - Pf6N2E2_2073 0.13 -0.3 2,466,454 - Pf6N2E2_2073 0.13 +0.1 2,466,454 - Pf6N2E2_2073 0.13 +0.2 2,466,454 - Pf6N2E2_2073 0.13 -0.7 2,466,454 - +0.3 2,466,454 - Pf6N2E2_2073 0.13 +0.2 2,466,454 - Pf6N2E2_2073 0.13 -0.8 2,466,454 - Pf6N2E2_2073 0.13 +1.3 2,466,496 - Pf6N2E2_2073 0.15 +0.6 2,466,550 - Pf6N2E2_2073 0.18 +0.0 2,466,594 + Pf6N2E2_2073 0.21 -1.8 2,466,595 - Pf6N2E2_2073 0.21 -0.3 2,466,888 + Pf6N2E2_2073 0.38 -0.9 2,466,888 + Pf6N2E2_2073 0.38 -0.3 2,466,889 - Pf6N2E2_2073 0.38 -0.8 2,466,920 - Pf6N2E2_2073 0.40 -0.8 2,466,945 - Pf6N2E2_2073 0.41 -0.4 2,467,026 + Pf6N2E2_2073 0.46 +0.7 2,467,026 + Pf6N2E2_2073 0.46 -2.8 2,467,027 - Pf6N2E2_2073 0.46 -0.5 2,467,027 - Pf6N2E2_2073 0.46 -0.7 2,467,027 - Pf6N2E2_2073 0.46 -0.5 2,467,209 + Pf6N2E2_2073 0.56 -0.7 2,467,317 + Pf6N2E2_2073 0.62 -1.0 2,467,648 - Pf6N2E2_2073 0.81 -0.8 2,467,764 + Pf6N2E2_2073 0.88 -0.2 2,467,764 + Pf6N2E2_2073 0.88 -0.6 2,467,878 + -0.4 2,467,878 + -1.0 2,467,878 + -0.9 2,467,879 - +1.5 2,467,892 + -1.8 2,467,892 + -1.8 2,467,920 + -3.2 2,467,920 + +0.3 2,467,920 + -0.6 2,467,921 - -0.7 2,467,921 - -1.8 2,467,921 - -0.6 2,467,928 + -1.3 2,467,928 + -0.5 2,467,928 + -0.6 2,467,928 + -1.5 2,467,959 + -1.3 2,467,959 + -1.6 2,467,960 - +0.7 2,468,314 + Pf6N2E2_2074 0.72 -0.5 2,468,315 - Pf6N2E2_2074 0.72 +0.5 2,468,407 + -1.2 2,468,407 + +0.7 2,468,408 - -0.4 2,468,603 - +0.0 2,468,603 - +1.0 2,468,615 - +0.5 2,468,615 - +0.6 2,468,723 - Pf6N2E2_2075 0.11 +0.2 2,468,735 - Pf6N2E2_2075 0.12 -1.3 2,468,873 - Pf6N2E2_2075 0.25 -0.8
Or see this region's nucleotide sequence