Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6108 and Pf6N2E2_2020 are separated by 63 nucleotides Pf6N2E2_2020 and Pf6N2E2_2021 are separated by 32 nucleotides Pf6N2E2_2021 and Pf6N2E2_2022 are separated by 68 nucleotides Pf6N2E2_2022 and Pf6N2E2_2023 are separated by 184 nucleotides
Pf6N2E2_6108 - tRNA-Gly-GCC, at 2,408,841 to 2,408,913
_6108
Pf6N2E2_2020 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 2,408,977 to 2,409,537
_2020
Pf6N2E2_2021 - Excinuclease ABC subunit C, at 2,409,570 to 2,411,327
_2021
Pf6N2E2_2022 - BarA-associated response regulator UvrY (= GacA = SirA), at 2,411,396 to 2,411,980
_2022
Pf6N2E2_2023 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 2,412,165 to 2,412,599
_2023
Position (kb)
2409
2410
2411
2412 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2408.620 kb on + strand at 2408.635 kb on + strand at 2408.635 kb on + strand at 2408.636 kb on - strand at 2408.676 kb on + strand at 2408.680 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.715 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.777 kb on - strand at 2408.935 kb on + strand at 2408.935 kb on + strand at 2408.936 kb on - strand at 2408.936 kb on - strand at 2409.579 kb on - strand at 2409.593 kb on - strand at 2409.593 kb on - strand at 2409.614 kb on + strand at 2409.737 kb on + strand at 2409.737 kb on + strand at 2409.738 kb on - strand at 2409.877 kb on + strand, within Pf6N2E2_2021 at 2409.877 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.082 kb on - strand, within Pf6N2E2_2021 at 2410.082 kb on - strand, within Pf6N2E2_2021 at 2410.114 kb on + strand, within Pf6N2E2_2021 at 2410.114 kb on + strand, within Pf6N2E2_2021 at 2410.204 kb on + strand, within Pf6N2E2_2021 at 2410.205 kb on - strand, within Pf6N2E2_2021 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.489 kb on + strand, within Pf6N2E2_2021 at 2410.835 kb on - strand, within Pf6N2E2_2021 at 2410.889 kb on - strand, within Pf6N2E2_2021 at 2410.918 kb on + strand, within Pf6N2E2_2021 at 2410.918 kb on + strand, within Pf6N2E2_2021 at 2410.919 kb on - strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.921 kb on + strand, within Pf6N2E2_2021 at 2410.922 kb on - strand, within Pf6N2E2_2021 at 2410.922 kb on - strand, within Pf6N2E2_2021 at 2411.011 kb on + strand, within Pf6N2E2_2021 at 2411.011 kb on + strand, within Pf6N2E2_2021 at 2411.083 kb on + strand, within Pf6N2E2_2021 at 2411.090 kb on - strand, within Pf6N2E2_2021 at 2411.119 kb on + strand, within Pf6N2E2_2021 at 2411.119 kb on + strand, within Pf6N2E2_2021 at 2411.257 kb on + strand at 2411.258 kb on - strand at 2411.299 kb on + strand at 2411.300 kb on - strand at 2411.480 kb on - strand, within Pf6N2E2_2022 at 2411.490 kb on + strand, within Pf6N2E2_2022 at 2411.490 kb on + strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.491 kb on - strand, within Pf6N2E2_2022 at 2411.540 kb on + strand, within Pf6N2E2_2022 at 2411.540 kb on + strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.541 kb on - strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.667 kb on + strand, within Pf6N2E2_2022 at 2411.668 kb on - strand, within Pf6N2E2_2022 at 2411.668 kb on - strand, within Pf6N2E2_2022 at 2411.951 kb on + strand at 2411.951 kb on + strand at 2411.952 kb on - strand at 2411.986 kb on + strand at 2411.986 kb on + strand at 2411.987 kb on - strand at 2411.987 kb on - strand at 2412.032 kb on - strand at 2412.083 kb on - strand at 2412.106 kb on - strand at 2412.122 kb on + strand at 2412.122 kb on + strand at 2412.123 kb on - strand at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.238 kb on + strand, within Pf6N2E2_2023 at 2412.239 kb on - strand, within Pf6N2E2_2023
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 2,408,620 + +2.1 2,408,635 + -1.5 2,408,635 + +0.3 2,408,636 - -1.6 2,408,676 + -0.7 2,408,680 + -1.0 2,408,712 + -0.9 2,408,712 + -1.3 2,408,712 + +0.5 2,408,712 + +0.3 2,408,712 + +0.9 2,408,712 + +1.5 2,408,712 + +0.2 2,408,713 - +0.2 2,408,713 - +1.2 2,408,713 - +0.5 2,408,713 - -0.0 2,408,715 + +1.4 2,408,776 + +0.0 2,408,776 + +0.1 2,408,776 + -0.3 2,408,776 + -0.3 2,408,776 + +0.0 2,408,776 + -0.2 2,408,777 - -0.4 2,408,935 + +0.7 2,408,935 + -0.6 2,408,936 - -0.7 2,408,936 - -1.4 2,409,579 - +1.3 2,409,593 - -2.4 2,409,593 - -0.1 2,409,614 + -1.5 2,409,737 + -0.4 2,409,737 + -0.6 2,409,738 - +0.0 2,409,877 + Pf6N2E2_2021 0.17 -0.4 2,409,877 + Pf6N2E2_2021 0.17 +0.5 2,410,081 + Pf6N2E2_2021 0.29 -1.6 2,410,081 + Pf6N2E2_2021 0.29 -1.5 2,410,081 + Pf6N2E2_2021 0.29 -0.1 2,410,082 - Pf6N2E2_2021 0.29 -0.1 2,410,082 - Pf6N2E2_2021 0.29 -1.8 2,410,114 + Pf6N2E2_2021 0.31 -1.8 2,410,114 + Pf6N2E2_2021 0.31 -1.3 2,410,204 + Pf6N2E2_2021 0.36 +1.7 2,410,205 - Pf6N2E2_2021 0.36 -0.3 2,410,486 + Pf6N2E2_2021 0.52 -2.9 2,410,486 + Pf6N2E2_2021 0.52 -0.1 2,410,487 - Pf6N2E2_2021 0.52 +0.6 2,410,487 - Pf6N2E2_2021 0.52 -0.5 2,410,487 - Pf6N2E2_2021 0.52 -0.6 2,410,489 + Pf6N2E2_2021 0.52 +0.0 2,410,835 - Pf6N2E2_2021 0.72 +0.2 2,410,889 - Pf6N2E2_2021 0.75 +0.1 2,410,918 + Pf6N2E2_2021 0.77 -1.1 2,410,918 + Pf6N2E2_2021 0.77 -1.4 2,410,919 - Pf6N2E2_2021 0.77 -0.9 2,410,921 + Pf6N2E2_2021 0.77 +0.9 2,410,921 + Pf6N2E2_2021 0.77 -0.2 2,410,921 + Pf6N2E2_2021 0.77 -0.3 2,410,922 - Pf6N2E2_2021 0.77 -1.9 2,410,922 - Pf6N2E2_2021 0.77 +1.6 2,411,011 + Pf6N2E2_2021 0.82 -0.7 2,411,011 + Pf6N2E2_2021 0.82 -0.9 2,411,083 + Pf6N2E2_2021 0.86 -2.3 2,411,090 - Pf6N2E2_2021 0.86 -1.4 2,411,119 + Pf6N2E2_2021 0.88 +0.3 2,411,119 + Pf6N2E2_2021 0.88 +1.5 2,411,257 + -1.1 2,411,258 - -0.6 2,411,299 + -3.1 2,411,300 - -0.3 2,411,480 - Pf6N2E2_2022 0.14 +1.9 2,411,490 + Pf6N2E2_2022 0.16 +0.1 2,411,490 + Pf6N2E2_2022 0.16 +1.3 2,411,491 - Pf6N2E2_2022 0.16 +1.3 2,411,491 - Pf6N2E2_2022 0.16 +0.7 2,411,491 - Pf6N2E2_2022 0.16 +0.1 2,411,491 - Pf6N2E2_2022 0.16 +0.7 2,411,540 + Pf6N2E2_2022 0.25 +0.6 2,411,540 + Pf6N2E2_2022 0.25 +1.2 2,411,541 - Pf6N2E2_2022 0.25 -1.3 2,411,541 - Pf6N2E2_2022 0.25 +0.3 2,411,541 - Pf6N2E2_2022 0.25 +1.0 2,411,667 + Pf6N2E2_2022 0.46 +1.7 2,411,667 + Pf6N2E2_2022 0.46 -0.3 2,411,667 + Pf6N2E2_2022 0.46 +0.7 2,411,667 + Pf6N2E2_2022 0.46 +0.2 2,411,667 + Pf6N2E2_2022 0.46 +0.6 2,411,668 - Pf6N2E2_2022 0.46 +0.9 2,411,668 - Pf6N2E2_2022 0.46 +2.0 2,411,951 + +0.6 2,411,951 + +1.3 2,411,952 - -0.0 2,411,986 + -0.1 2,411,986 + -0.7 2,411,987 - +0.8 2,411,987 - +2.2 2,412,032 - +1.0 2,412,083 - +0.9 2,412,106 - -0.1 2,412,122 + +0.5 2,412,122 + +1.3 2,412,123 - -0.7 2,412,238 + Pf6N2E2_2023 0.17 -1.2 2,412,238 + Pf6N2E2_2023 0.17 +0.0 2,412,238 + Pf6N2E2_2023 0.17 -2.6 2,412,239 - Pf6N2E2_2023 0.17 -0.0
Or see this region's nucleotide sequence