Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1624

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1623 and Pf6N2E2_1624 are separated by 36 nucleotidesPf6N2E2_1624 and Pf6N2E2_1625 are separated by 203 nucleotidesPf6N2E2_1625 and Pf6N2E2_1626 are separated by 7 nucleotides Pf6N2E2_1623 - Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76), at 1,899,027 to 1,900,457 _1623 Pf6N2E2_1624 - MbtH-like protein, at 1,900,494 to 1,900,709 _1624 Pf6N2E2_1625 - Ferric reductase (1.6.99.14), at 1,900,913 to 1,901,650 _1625 Pf6N2E2_1626 - Putative ATP-binding component of a transport system, at 1,901,658 to 1,903,301 _1626 Position (kb) 1900 1901Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1899.552 kb on + strand, within Pf6N2E2_1623at 1899.553 kb on - strand, within Pf6N2E2_1623at 1899.691 kb on - strand, within Pf6N2E2_1623at 1900.276 kb on + strand, within Pf6N2E2_1623at 1900.276 kb on + strand, within Pf6N2E2_1623at 1900.277 kb on - strand, within Pf6N2E2_1623at 1900.277 kb on - strand, within Pf6N2E2_1623at 1900.434 kb on + strandat 1900.434 kb on + strandat 1900.435 kb on - strandat 1900.435 kb on - strandat 1900.473 kb on + strandat 1900.509 kb on + strandat 1900.509 kb on + strandat 1900.510 kb on - strandat 1900.510 kb on - strandat 1900.567 kb on + strand, within Pf6N2E2_1624at 1900.567 kb on + strand, within Pf6N2E2_1624at 1900.568 kb on - strand, within Pf6N2E2_1624at 1900.568 kb on - strand, within Pf6N2E2_1624at 1900.672 kb on - strand, within Pf6N2E2_1624at 1900.672 kb on - strand, within Pf6N2E2_1624at 1900.751 kb on - strandat 1900.855 kb on - strandat 1900.855 kb on - strandat 1900.856 kb on + strandat 1900.856 kb on + strandat 1900.857 kb on - strandat 1900.857 kb on - strandat 1901.001 kb on + strand, within Pf6N2E2_1625at 1901.031 kb on - strand, within Pf6N2E2_1625at 1901.291 kb on - strand, within Pf6N2E2_1625at 1901.383 kb on + strand, within Pf6N2E2_1625at 1901.384 kb on - strand, within Pf6N2E2_1625at 1901.648 kb on + strandat 1901.648 kb on + strandat 1901.659 kb on + strandat 1901.698 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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1,899,552 + Pf6N2E2_1623 0.37 -0.7
1,899,553 - Pf6N2E2_1623 0.37 -3.1
1,899,691 - Pf6N2E2_1623 0.46 +0.9
1,900,276 + Pf6N2E2_1623 0.87 -0.7
1,900,276 + Pf6N2E2_1623 0.87 -1.3
1,900,277 - Pf6N2E2_1623 0.87 +0.8
1,900,277 - Pf6N2E2_1623 0.87 +0.2
1,900,434 + -0.7
1,900,434 + -1.1
1,900,435 - -0.9
1,900,435 - -0.2
1,900,473 + -0.5
1,900,509 + -1.2
1,900,509 + -1.0
1,900,510 - +0.2
1,900,510 - +0.6
1,900,567 + Pf6N2E2_1624 0.34 -1.5
1,900,567 + Pf6N2E2_1624 0.34 -2.6
1,900,568 - Pf6N2E2_1624 0.34 +0.3
1,900,568 - Pf6N2E2_1624 0.34 +0.4
1,900,672 - Pf6N2E2_1624 0.82 -1.6
1,900,672 - Pf6N2E2_1624 0.82 -1.4
1,900,751 - +0.8
1,900,855 - +1.2
1,900,855 - +1.0
1,900,856 + +1.3
1,900,856 + -3.0
1,900,857 - +2.0
1,900,857 - +0.9
1,901,001 + Pf6N2E2_1625 0.12 +0.8
1,901,031 - Pf6N2E2_1625 0.16 +1.4
1,901,291 - Pf6N2E2_1625 0.51 +0.6
1,901,383 + Pf6N2E2_1625 0.64 +0.7
1,901,384 - Pf6N2E2_1625 0.64 +0.4
1,901,648 + +1.4
1,901,648 + +1.2
1,901,659 + +0.7
1,901,698 + +2.0

Or see this region's nucleotide sequence