Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1623 and Pf6N2E2_1624 are separated by 36 nucleotides Pf6N2E2_1624 and Pf6N2E2_1625 are separated by 203 nucleotides Pf6N2E2_1625 and Pf6N2E2_1626 are separated by 7 nucleotides
Pf6N2E2_1623 - Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76), at 1,899,027 to 1,900,457
_1623
Pf6N2E2_1624 - MbtH-like protein, at 1,900,494 to 1,900,709
_1624
Pf6N2E2_1625 - Ferric reductase (1.6.99.14), at 1,900,913 to 1,901,650
_1625
Pf6N2E2_1626 - Putative ATP-binding component of a transport system, at 1,901,658 to 1,903,301
_1626
Position (kb)
1900
1901 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1899.552 kb on + strand, within Pf6N2E2_1623 at 1899.553 kb on - strand, within Pf6N2E2_1623 at 1899.691 kb on - strand, within Pf6N2E2_1623 at 1900.276 kb on + strand, within Pf6N2E2_1623 at 1900.276 kb on + strand, within Pf6N2E2_1623 at 1900.277 kb on - strand, within Pf6N2E2_1623 at 1900.277 kb on - strand, within Pf6N2E2_1623 at 1900.434 kb on + strand at 1900.434 kb on + strand at 1900.435 kb on - strand at 1900.435 kb on - strand at 1900.473 kb on + strand at 1900.509 kb on + strand at 1900.509 kb on + strand at 1900.510 kb on - strand at 1900.510 kb on - strand at 1900.567 kb on + strand, within Pf6N2E2_1624 at 1900.567 kb on + strand, within Pf6N2E2_1624 at 1900.568 kb on - strand, within Pf6N2E2_1624 at 1900.568 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.751 kb on - strand at 1900.855 kb on - strand at 1900.855 kb on - strand at 1900.856 kb on + strand at 1900.856 kb on + strand at 1900.857 kb on - strand at 1900.857 kb on - strand at 1901.001 kb on + strand, within Pf6N2E2_1625 at 1901.031 kb on - strand, within Pf6N2E2_1625 at 1901.291 kb on - strand, within Pf6N2E2_1625 at 1901.383 kb on + strand, within Pf6N2E2_1625 at 1901.384 kb on - strand, within Pf6N2E2_1625 at 1901.648 kb on + strand at 1901.648 kb on + strand at 1901.659 kb on + strand at 1901.698 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 1,899,552 + Pf6N2E2_1623 0.37 -0.7 1,899,553 - Pf6N2E2_1623 0.37 -3.1 1,899,691 - Pf6N2E2_1623 0.46 +0.9 1,900,276 + Pf6N2E2_1623 0.87 -0.7 1,900,276 + Pf6N2E2_1623 0.87 -1.3 1,900,277 - Pf6N2E2_1623 0.87 +0.8 1,900,277 - Pf6N2E2_1623 0.87 +0.2 1,900,434 + -0.7 1,900,434 + -1.1 1,900,435 - -0.9 1,900,435 - -0.2 1,900,473 + -0.5 1,900,509 + -1.2 1,900,509 + -1.0 1,900,510 - +0.2 1,900,510 - +0.6 1,900,567 + Pf6N2E2_1624 0.34 -1.5 1,900,567 + Pf6N2E2_1624 0.34 -2.6 1,900,568 - Pf6N2E2_1624 0.34 +0.3 1,900,568 - Pf6N2E2_1624 0.34 +0.4 1,900,672 - Pf6N2E2_1624 0.82 -1.6 1,900,672 - Pf6N2E2_1624 0.82 -1.4 1,900,751 - +0.8 1,900,855 - +1.2 1,900,855 - +1.0 1,900,856 + +1.3 1,900,856 + -3.0 1,900,857 - +2.0 1,900,857 - +0.9 1,901,001 + Pf6N2E2_1625 0.12 +0.8 1,901,031 - Pf6N2E2_1625 0.16 +1.4 1,901,291 - Pf6N2E2_1625 0.51 +0.6 1,901,383 + Pf6N2E2_1625 0.64 +0.7 1,901,384 - Pf6N2E2_1625 0.64 +0.4 1,901,648 + +1.4 1,901,648 + +1.2 1,901,659 + +0.7 1,901,698 + +2.0
Or see this region's nucleotide sequence