Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_154 and Pf6N2E2_155 are separated by 198 nucleotides Pf6N2E2_155 and Pf6N2E2_156 are separated by 190 nucleotides Pf6N2E2_156 and Pf6N2E2_157 are separated by 247 nucleotides Pf6N2E2_157 and Pf6N2E2_158 are separated by 63 nucleotides
Pf6N2E2_154 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 209,411 to 210,793
_154
Pf6N2E2_155 - Probable transmembrane protein, at 210,992 to 211,447
_155
Pf6N2E2_156 - Carbon storage regulator, at 211,638 to 211,832
_156
Pf6N2E2_157 - hypothetical protein, at 212,080 to 212,445
_157
Pf6N2E2_158 - Endonuclease I precursor (EC 3.1.21.1), at 212,509 to 213,198
_158
Position (kb) 211
212 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 210.791 kb on + strand at 210.791 kb on + strand at 210.791 kb on + strand at 210.792 kb on - strand at 210.792 kb on - strand at 210.792 kb on - strand at 210.801 kb on + strand at 210.801 kb on + strand at 210.802 kb on - strand at 210.802 kb on - strand at 210.802 kb on - strand at 210.877 kb on - strand at 210.941 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.948 kb on + strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.949 kb on - strand at 210.951 kb on + strand at 211.078 kb on - strand, within Pf6N2E2_155 at 211.211 kb on + strand, within Pf6N2E2_155 at 211.211 kb on + strand, within Pf6N2E2_155 at 211.212 kb on - strand, within Pf6N2E2_155 at 211.215 kb on - strand, within Pf6N2E2_155 at 211.477 kb on + strand at 211.477 kb on + strand at 211.503 kb on + strand at 211.518 kb on + strand at 211.518 kb on + strand at 211.519 kb on - strand at 211.567 kb on + strand at 211.683 kb on - strand, within Pf6N2E2_156 at 211.797 kb on + strand, within Pf6N2E2_156 at 211.797 kb on + strand, within Pf6N2E2_156 at 211.848 kb on + strand at 211.848 kb on + strand at 211.848 kb on + strand at 211.849 kb on - strand at 211.849 kb on - strand at 211.849 kb on - strand at 211.849 kb on - strand at 211.853 kb on + strand at 211.853 kb on + strand at 211.853 kb on + strand at 211.854 kb on - strand at 211.854 kb on - strand at 211.854 kb on - strand at 211.855 kb on + strand at 211.855 kb on + strand at 211.856 kb on - strand at 211.896 kb on - strand at 211.896 kb on - strand at 211.897 kb on + strand at 211.897 kb on + strand at 211.897 kb on + strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.898 kb on - strand at 211.903 kb on - strand at 211.903 kb on - strand at 211.908 kb on + strand at 211.908 kb on + strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.909 kb on - strand at 211.921 kb on - strand at 211.921 kb on - strand at 211.955 kb on + strand at 211.955 kb on + strand at 211.975 kb on + strand at 211.976 kb on - strand at 211.976 kb on - strand at 211.976 kb on - strand at 211.976 kb on - strand at 212.044 kb on + strand at 212.045 kb on - strand at 212.108 kb on + strand at 212.634 kb on - strand, within Pf6N2E2_158 at 212.670 kb on - strand, within Pf6N2E2_158 at 212.684 kb on + strand, within Pf6N2E2_158 at 212.684 kb on + strand, within Pf6N2E2_158 at 212.685 kb on - strand, within Pf6N2E2_158 at 212.685 kb on - strand, within Pf6N2E2_158 at 212.685 kb on - strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.690 kb on + strand, within Pf6N2E2_158 at 212.691 kb on - strand, within Pf6N2E2_158 at 212.691 kb on - strand, within Pf6N2E2_158
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 210,791 + -0.7 210,791 + +0.9 210,791 + +0.6 210,792 - -0.4 210,792 - +1.7 210,792 - -0.2 210,801 + +0.1 210,801 + +1.0 210,802 - +0.2 210,802 - -0.3 210,802 - +1.7 210,877 - +1.1 210,941 + -0.4 210,948 + +0.7 210,948 + +0.8 210,948 + -0.5 210,948 + -0.6 210,948 + -0.2 210,948 + -0.2 210,948 + +0.6 210,948 + -0.0 210,948 + +0.0 210,948 + -0.7 210,949 - -0.7 210,949 - +0.5 210,949 - -1.1 210,949 - -0.9 210,949 - -0.7 210,949 - -0.4 210,949 - -0.2 210,949 - +0.0 210,949 - -0.5 210,951 + +0.0 211,078 - Pf6N2E2_155 0.19 -1.2 211,211 + Pf6N2E2_155 0.48 +0.4 211,211 + Pf6N2E2_155 0.48 +0.1 211,212 - Pf6N2E2_155 0.48 +0.8 211,215 - Pf6N2E2_155 0.49 -0.3 211,477 + +1.4 211,477 + +1.3 211,503 + +0.8 211,518 + -2.8 211,518 + -0.9 211,519 - -0.0 211,567 + -0.0 211,683 - Pf6N2E2_156 0.23 -0.2 211,797 + Pf6N2E2_156 0.82 -0.6 211,797 + Pf6N2E2_156 0.82 -2.2 211,848 + -0.7 211,848 + +0.2 211,848 + +0.4 211,849 - -0.2 211,849 - +0.1 211,849 - +0.2 211,849 - +0.1 211,853 + -0.9 211,853 + -2.1 211,853 + -2.8 211,854 - -0.0 211,854 - -0.2 211,854 - +0.1 211,855 + -0.7 211,855 + -1.2 211,856 - +0.4 211,896 - +1.4 211,896 - -0.1 211,897 + -0.5 211,897 + -0.8 211,897 + -0.7 211,898 - -0.2 211,898 - +0.8 211,898 - +0.4 211,898 - +0.8 211,898 - +0.8 211,898 - +1.1 211,898 - -1.9 211,898 - +0.1 211,903 - +1.1 211,903 - +1.1 211,908 + +1.0 211,908 + -0.1 211,909 - +0.2 211,909 - -0.6 211,909 - -0.4 211,909 - +1.3 211,909 - -0.4 211,909 - +0.5 211,921 - -0.0 211,921 - +0.0 211,955 + -0.9 211,955 + -0.2 211,975 + -0.3 211,976 - -0.1 211,976 - +0.3 211,976 - +0.8 211,976 - +0.6 212,044 + -0.8 212,045 - +0.5 212,108 + -0.9 212,634 - Pf6N2E2_158 0.18 +1.5 212,670 - Pf6N2E2_158 0.23 +0.6 212,684 + Pf6N2E2_158 0.25 -1.3 212,684 + Pf6N2E2_158 0.25 -1.2 212,685 - Pf6N2E2_158 0.26 -0.4 212,685 - Pf6N2E2_158 0.26 +0.8 212,685 - Pf6N2E2_158 0.26 +0.1 212,690 + Pf6N2E2_158 0.26 +2.3 212,690 + Pf6N2E2_158 0.26 -0.9 212,690 + Pf6N2E2_158 0.26 +0.1 212,690 + Pf6N2E2_158 0.26 -0.0 212,690 + Pf6N2E2_158 0.26 +0.6 212,691 - Pf6N2E2_158 0.26 +0.6 212,691 - Pf6N2E2_158 0.26 +0.5
Or see this region's nucleotide sequence