Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1440 and Pf6N2E2_1441 are separated by 15 nucleotides Pf6N2E2_1441 and Pf6N2E2_1442 are separated by 120 nucleotides
Pf6N2E2_1440 - Putative tRNA-m1A22 methylase, at 1,690,418 to 1,691,113
_1440
Pf6N2E2_1441 - Uncharacterized oxidoreductase ydgJ (EC 1.-.-.-), at 1,691,129 to 1,692,055
_1441
Pf6N2E2_1442 - Transcriptional regulators of sugar metabolism, at 1,692,176 to 1,692,970
_1442
Position (kb)
1691
1692
1693 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1690.227 kb on + strand at 1690.228 kb on - strand at 1690.277 kb on - strand at 1690.277 kb on - strand at 1690.330 kb on - strand at 1690.330 kb on - strand at 1690.340 kb on + strand at 1690.351 kb on - strand at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.596 kb on + strand, within Pf6N2E2_1440 at 1690.614 kb on + strand, within Pf6N2E2_1440 at 1690.615 kb on - strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.751 kb on + strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440 at 1690.752 kb on - strand, within Pf6N2E2_1440 at 1691.020 kb on - strand, within Pf6N2E2_1440 at 1691.065 kb on + strand at 1691.066 kb on - strand at 1691.066 kb on - strand at 1691.066 kb on - strand at 1691.137 kb on - strand at 1691.251 kb on - strand, within Pf6N2E2_1441 at 1691.354 kb on + strand, within Pf6N2E2_1441 at 1691.354 kb on + strand, within Pf6N2E2_1441 at 1691.355 kb on - strand, within Pf6N2E2_1441 at 1692.079 kb on - strand at 1692.101 kb on - strand at 1692.139 kb on + strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.140 kb on - strand at 1692.170 kb on - strand at 1692.182 kb on - strand at 1692.182 kb on - strand at 1692.182 kb on - strand at 1692.228 kb on + strand at 1692.228 kb on + strand at 1692.462 kb on + strand, within Pf6N2E2_1442 at 1692.463 kb on - strand, within Pf6N2E2_1442 at 1692.749 kb on + strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.750 kb on - strand, within Pf6N2E2_1442 at 1692.783 kb on - strand, within Pf6N2E2_1442 at 1692.795 kb on + strand, within Pf6N2E2_1442 at 1692.809 kb on + strand, within Pf6N2E2_1442 at 1693.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 1,690,227 + -0.6 1,690,228 - -1.0 1,690,277 - +0.3 1,690,277 - +0.2 1,690,330 - +1.2 1,690,330 - -0.8 1,690,340 + -0.3 1,690,351 - +0.4 1,690,596 + Pf6N2E2_1440 0.26 +1.2 1,690,596 + Pf6N2E2_1440 0.26 +0.8 1,690,614 + Pf6N2E2_1440 0.28 +0.7 1,690,615 - Pf6N2E2_1440 0.28 +1.0 1,690,751 + Pf6N2E2_1440 0.48 +1.7 1,690,751 + Pf6N2E2_1440 0.48 +2.1 1,690,752 - Pf6N2E2_1440 0.48 -0.3 1,690,752 - Pf6N2E2_1440 0.48 -1.4 1,691,020 - Pf6N2E2_1440 0.86 -0.0 1,691,065 + -0.1 1,691,066 - -0.5 1,691,066 - -0.2 1,691,066 - -0.3 1,691,137 - +0.5 1,691,251 - Pf6N2E2_1441 0.13 -2.3 1,691,354 + Pf6N2E2_1441 0.24 +0.6 1,691,354 + Pf6N2E2_1441 0.24 -0.8 1,691,355 - Pf6N2E2_1441 0.24 -1.1 1,692,079 - -1.5 1,692,101 - +0.8 1,692,139 + +1.1 1,692,140 - -0.3 1,692,140 - +0.3 1,692,140 - +1.7 1,692,140 - +0.1 1,692,140 - -0.2 1,692,140 - -0.1 1,692,170 - -0.3 1,692,182 - +1.2 1,692,182 - +0.7 1,692,182 - +0.2 1,692,228 + +0.2 1,692,228 + +0.3 1,692,462 + Pf6N2E2_1442 0.36 +0.2 1,692,463 - Pf6N2E2_1442 0.36 +1.1 1,692,749 + Pf6N2E2_1442 0.72 -0.8 1,692,750 - Pf6N2E2_1442 0.72 -0.9 1,692,750 - Pf6N2E2_1442 0.72 -1.3 1,692,750 - Pf6N2E2_1442 0.72 +0.4 1,692,783 - Pf6N2E2_1442 0.76 +0.5 1,692,795 + Pf6N2E2_1442 0.78 +0.2 1,692,809 + Pf6N2E2_1442 0.80 -0.7 1,693,029 - +1.9
Or see this region's nucleotide sequence