Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1102 and Pf6N2E2_1103 are separated by 11 nucleotides Pf6N2E2_1103 and Pf6N2E2_1104 overlap by 4 nucleotides
Pf6N2E2_1102 - Aldehyde dehydrogenase A (EC 1.2.1.22), at 1,288,309 to 1,289,742
_1102
Pf6N2E2_1103 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,289,754 to 1,290,554
_1103
Pf6N2E2_1104 - Gluconate dehydratase (EC 4.2.1.39), at 1,290,551 to 1,291,750
_1104
Position (kb)
1289
1290
1291 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.409 kb on - strand, within Pf6N2E2_1102 at 1289.409 kb on - strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.418 kb on - strand, within Pf6N2E2_1102 at 1289.418 kb on - strand, within Pf6N2E2_1102 at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.670 kb on - strand at 1289.758 kb on + strand at 1289.759 kb on - strand at 1289.973 kb on + strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1290.145 kb on + strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.359 kb on - strand, within Pf6N2E2_1103 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.777 kb on + strand, within Pf6N2E2_1104 at 1290.777 kb on + strand, within Pf6N2E2_1104 at 1290.783 kb on + strand, within Pf6N2E2_1104 at 1290.783 kb on + strand, within Pf6N2E2_1104 at 1290.784 kb on - strand, within Pf6N2E2_1104 at 1290.807 kb on - strand, within Pf6N2E2_1104 at 1290.977 kb on + strand, within Pf6N2E2_1104 at 1291.083 kb on + strand, within Pf6N2E2_1104 at 1291.084 kb on - strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.426 kb on - strand, within Pf6N2E2_1104 at 1291.503 kb on + strand, within Pf6N2E2_1104
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 1,288,877 + Pf6N2E2_1102 0.40 -0.3 1,288,877 + Pf6N2E2_1102 0.40 -0.2 1,288,877 + Pf6N2E2_1102 0.40 -0.2 1,289,408 + Pf6N2E2_1102 0.77 +0.7 1,289,408 + Pf6N2E2_1102 0.77 +0.9 1,289,408 + Pf6N2E2_1102 0.77 +0.1 1,289,409 - Pf6N2E2_1102 0.77 -1.9 1,289,409 - Pf6N2E2_1102 0.77 +1.3 1,289,417 + Pf6N2E2_1102 0.77 +0.2 1,289,417 + Pf6N2E2_1102 0.77 -0.4 1,289,417 + Pf6N2E2_1102 0.77 -0.5 1,289,417 + Pf6N2E2_1102 0.77 -0.7 1,289,417 + Pf6N2E2_1102 0.77 +1.1 1,289,418 - Pf6N2E2_1102 0.77 -0.3 1,289,418 - Pf6N2E2_1102 0.77 +2.2 1,289,669 + +0.1 1,289,669 + -0.5 1,289,669 + +0.7 1,289,669 + +0.6 1,289,669 + +1.0 1,289,670 - -0.6 1,289,758 + -0.2 1,289,759 - +0.1 1,289,973 + Pf6N2E2_1103 0.27 -0.6 1,289,974 - Pf6N2E2_1103 0.27 +0.2 1,289,974 - Pf6N2E2_1103 0.27 -1.5 1,290,145 + Pf6N2E2_1103 0.49 +0.5 1,290,146 - Pf6N2E2_1103 0.49 -0.2 1,290,146 - Pf6N2E2_1103 0.49 -1.4 1,290,146 - Pf6N2E2_1103 0.49 -1.8 1,290,359 - Pf6N2E2_1103 0.76 -0.4 1,290,755 - Pf6N2E2_1104 0.17 +0.6 1,290,755 - Pf6N2E2_1104 0.17 -1.4 1,290,755 - Pf6N2E2_1104 0.17 +0.8 1,290,777 + Pf6N2E2_1104 0.19 +2.0 1,290,777 + Pf6N2E2_1104 0.19 +1.2 1,290,783 + Pf6N2E2_1104 0.19 -1.5 1,290,783 + Pf6N2E2_1104 0.19 +1.0 1,290,784 - Pf6N2E2_1104 0.19 +2.1 1,290,807 - Pf6N2E2_1104 0.21 -0.5 1,290,977 + Pf6N2E2_1104 0.35 +0.9 1,291,083 + Pf6N2E2_1104 0.44 +0.1 1,291,084 - Pf6N2E2_1104 0.44 -1.1 1,291,410 + Pf6N2E2_1104 0.72 +0.2 1,291,410 + Pf6N2E2_1104 0.72 -0.7 1,291,410 + Pf6N2E2_1104 0.72 -2.4 1,291,410 + Pf6N2E2_1104 0.72 -0.9 1,291,425 + Pf6N2E2_1104 0.73 -0.7 1,291,425 + Pf6N2E2_1104 0.73 -0.5 1,291,425 + Pf6N2E2_1104 0.73 -0.4 1,291,425 + Pf6N2E2_1104 0.73 +1.5 1,291,426 - Pf6N2E2_1104 0.73 -0.2 1,291,503 + Pf6N2E2_1104 0.79 +1.1
Or see this region's nucleotide sequence