Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_104 and Pf6N2E2_105 overlap by 4 nucleotides Pf6N2E2_105 and Pf6N2E2_106 are separated by 10 nucleotides Pf6N2E2_106 and Pf6N2E2_107 are separated by 10 nucleotides Pf6N2E2_107 and Pf6N2E2_108 are separated by 48 nucleotides
Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747
_104
Pf6N2E2_105 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 150,744 to 151,610
_105
Pf6N2E2_106 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 151,621 to 152,421
_106
Pf6N2E2_107 - Probable transmembrane protein, at 152,432 to 152,704
_107
Pf6N2E2_108 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 152,753 to 154,495
_108
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 150.627 kb on - strand, within Pf6N2E2_104 at 150.667 kb on + strand at 150.667 kb on + strand at 150.667 kb on + strand at 150.701 kb on - strand at 150.712 kb on + strand at 150.712 kb on + strand at 150.879 kb on + strand, within Pf6N2E2_105 at 151.167 kb on + strand, within Pf6N2E2_105 at 151.167 kb on + strand, within Pf6N2E2_105 at 151.168 kb on - strand, within Pf6N2E2_105 at 151.168 kb on - strand, within Pf6N2E2_105 at 151.173 kb on + strand, within Pf6N2E2_105 at 151.173 kb on + strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.258 kb on - strand, within Pf6N2E2_105 at 151.287 kb on + strand, within Pf6N2E2_105 at 151.288 kb on - strand, within Pf6N2E2_105 at 151.288 kb on - strand, within Pf6N2E2_105 at 151.290 kb on + strand, within Pf6N2E2_105 at 151.290 kb on + strand at 151.290 kb on + strand, within Pf6N2E2_105 at 151.291 kb on - strand, within Pf6N2E2_105 at 151.413 kb on + strand, within Pf6N2E2_105 at 151.414 kb on - strand, within Pf6N2E2_105 at 151.540 kb on - strand at 151.540 kb on - strand at 151.540 kb on - strand at 151.540 kb on - strand at 151.575 kb on + strand at 151.575 kb on + strand at 151.654 kb on + strand at 151.655 kb on - strand at 151.655 kb on - strand at 151.655 kb on - strand at 151.681 kb on + strand at 151.695 kb on + strand at 151.695 kb on + strand at 151.696 kb on - strand at 151.768 kb on + strand, within Pf6N2E2_106 at 151.768 kb on + strand, within Pf6N2E2_106 at 151.768 kb on + strand, within Pf6N2E2_106 at 151.769 kb on - strand, within Pf6N2E2_106 at 151.798 kb on + strand, within Pf6N2E2_106 at 151.823 kb on - strand, within Pf6N2E2_106 at 151.825 kb on + strand, within Pf6N2E2_106 at 151.826 kb on - strand, within Pf6N2E2_106 at 151.826 kb on - strand, within Pf6N2E2_106 at 151.984 kb on + strand, within Pf6N2E2_106 at 151.984 kb on + strand, within Pf6N2E2_106 at 151.985 kb on - strand, within Pf6N2E2_106 at 151.985 kb on - strand, within Pf6N2E2_106 at 152.117 kb on - strand, within Pf6N2E2_106 at 152.703 kb on - strand at 152.731 kb on + strand at 152.752 kb on + strand at 152.753 kb on - strand at 152.753 kb on - strand at 152.753 kb on - strand at 152.753 kb on - strand at 152.782 kb on + strand at 152.783 kb on - strand at 152.783 kb on - strand at 152.893 kb on + strand at 152.893 kb on + strand at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.131 kb on + strand, within Pf6N2E2_108 at 153.131 kb on + strand, within Pf6N2E2_108 at 153.131 kb on + strand, within Pf6N2E2_108 at 153.131 kb on + strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.268 kb on + strand, within Pf6N2E2_108 at 153.278 kb on - strand, within Pf6N2E2_108 at 153.336 kb on - strand, within Pf6N2E2_108 at 153.395 kb on + strand, within Pf6N2E2_108 at 153.395 kb on + strand, within Pf6N2E2_108 at 153.396 kb on - strand, within Pf6N2E2_108 at 153.396 kb on - strand, within Pf6N2E2_108 at 153.396 kb on - strand, within Pf6N2E2_108
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 150,627 - Pf6N2E2_104 0.89 -1.0 150,667 + -0.1 150,667 + +0.2 150,667 + +0.7 150,701 - -0.0 150,712 + -0.5 150,712 + +1.2 150,879 + Pf6N2E2_105 0.16 +1.1 151,167 + Pf6N2E2_105 0.49 +0.8 151,167 + Pf6N2E2_105 0.49 -0.6 151,168 - Pf6N2E2_105 0.49 +1.5 151,168 - Pf6N2E2_105 0.49 -0.3 151,173 + Pf6N2E2_105 0.49 -0.3 151,173 + Pf6N2E2_105 0.49 +0.6 151,174 - Pf6N2E2_105 0.50 +0.1 151,174 - Pf6N2E2_105 0.50 -0.3 151,174 - Pf6N2E2_105 0.50 +0.8 151,258 - Pf6N2E2_105 0.59 -2.7 151,287 + Pf6N2E2_105 0.63 +1.4 151,288 - Pf6N2E2_105 0.63 -0.2 151,288 - Pf6N2E2_105 0.63 +1.0 151,290 + Pf6N2E2_105 0.63 +0.9 151,290 + -0.2 151,290 + Pf6N2E2_105 0.63 +0.1 151,291 - Pf6N2E2_105 0.63 +0.4 151,413 + Pf6N2E2_105 0.77 -0.4 151,414 - Pf6N2E2_105 0.77 -1.2 151,540 - +1.7 151,540 - +0.2 151,540 - +1.0 151,540 - +0.1 151,575 + +1.1 151,575 + +2.1 151,654 + +0.8 151,655 - -0.2 151,655 - +0.1 151,655 - -0.7 151,681 + +0.3 151,695 + +0.3 151,695 + +0.5 151,696 - +0.6 151,768 + Pf6N2E2_106 0.18 -0.3 151,768 + Pf6N2E2_106 0.18 +1.4 151,768 + Pf6N2E2_106 0.18 -0.2 151,769 - Pf6N2E2_106 0.18 -0.1 151,798 + Pf6N2E2_106 0.22 -0.7 151,823 - Pf6N2E2_106 0.25 -0.7 151,825 + Pf6N2E2_106 0.25 +0.9 151,826 - Pf6N2E2_106 0.26 +0.8 151,826 - Pf6N2E2_106 0.26 -0.3 151,984 + Pf6N2E2_106 0.45 +0.0 151,984 + Pf6N2E2_106 0.45 +0.5 151,985 - Pf6N2E2_106 0.45 -1.5 151,985 - Pf6N2E2_106 0.45 -1.9 152,117 - Pf6N2E2_106 0.62 -0.8 152,703 - -0.2 152,731 + +0.6 152,752 + -0.4 152,753 - -0.4 152,753 - +0.7 152,753 - +0.8 152,753 - -0.6 152,782 + +0.8 152,783 - +0.5 152,783 - +0.1 152,893 + -1.1 152,893 + -0.3 153,127 + Pf6N2E2_108 0.21 +0.5 153,127 + Pf6N2E2_108 0.21 -1.0 153,127 + Pf6N2E2_108 0.21 -0.3 153,127 + Pf6N2E2_108 0.21 -0.0 153,127 + Pf6N2E2_108 0.21 +0.5 153,127 + Pf6N2E2_108 0.21 -1.4 153,127 + Pf6N2E2_108 0.21 +1.0 153,128 - Pf6N2E2_108 0.22 +3.4 153,128 - Pf6N2E2_108 0.22 -0.0 153,128 - Pf6N2E2_108 0.22 -1.7 153,128 - Pf6N2E2_108 0.22 +0.4 153,128 - Pf6N2E2_108 0.22 -0.6 153,128 - Pf6N2E2_108 0.22 -0.6 153,128 - Pf6N2E2_108 0.22 -0.2 153,131 + Pf6N2E2_108 0.22 -0.0 153,131 + Pf6N2E2_108 0.22 -0.4 153,131 + Pf6N2E2_108 0.22 +0.4 153,131 + Pf6N2E2_108 0.22 +1.0 153,132 - Pf6N2E2_108 0.22 -0.3 153,132 - Pf6N2E2_108 0.22 -1.2 153,132 - Pf6N2E2_108 0.22 +1.0 153,132 - Pf6N2E2_108 0.22 +0.4 153,132 - Pf6N2E2_108 0.22 -0.2 153,132 - Pf6N2E2_108 0.22 +0.5 153,132 - Pf6N2E2_108 0.22 +0.1 153,132 - Pf6N2E2_108 0.22 -1.2 153,132 - Pf6N2E2_108 0.22 -0.7 153,132 - Pf6N2E2_108 0.22 +0.2 153,268 + Pf6N2E2_108 0.30 +0.1 153,278 - Pf6N2E2_108 0.30 +0.4 153,336 - Pf6N2E2_108 0.33 +0.3 153,395 + Pf6N2E2_108 0.37 +0.0 153,395 + Pf6N2E2_108 0.37 +0.5 153,396 - Pf6N2E2_108 0.37 +0.5 153,396 - Pf6N2E2_108 0.37 +0.4 153,396 - Pf6N2E2_108 0.37 +0.1
Or see this region's nucleotide sequence