Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1055 and Pf6N2E2_1056 are separated by 257 nucleotides Pf6N2E2_1056 and Pf6N2E2_1057 are separated by 705 nucleotides
Pf6N2E2_1055 - Transcriptional regulator MexT, at 1,229,514 to 1,230,428
_1055
Pf6N2E2_1056 - Quinone oxidoreductase (EC 1.6.5.5), at 1,230,686 to 1,231,711
_1056
Pf6N2E2_1057 - hypothetical protein, at 1,232,417 to 1,233,883
_1057
Position (kb)
1230
1231
1232 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1229.687 kb on - strand, within Pf6N2E2_1055 at 1229.687 kb on - strand, within Pf6N2E2_1055 at 1229.804 kb on + strand, within Pf6N2E2_1055 at 1229.901 kb on + strand, within Pf6N2E2_1055 at 1229.901 kb on + strand, within Pf6N2E2_1055 at 1229.902 kb on - strand, within Pf6N2E2_1055 at 1229.902 kb on - strand, within Pf6N2E2_1055 at 1229.930 kb on + strand, within Pf6N2E2_1055 at 1229.930 kb on + strand, within Pf6N2E2_1055 at 1229.931 kb on - strand, within Pf6N2E2_1055 at 1229.931 kb on - strand, within Pf6N2E2_1055 at 1229.963 kb on - strand, within Pf6N2E2_1055 at 1229.963 kb on - strand, within Pf6N2E2_1055 at 1230.016 kb on + strand, within Pf6N2E2_1055 at 1230.016 kb on + strand, within Pf6N2E2_1055 at 1230.016 kb on + strand, within Pf6N2E2_1055 at 1230.016 kb on + strand, within Pf6N2E2_1055 at 1230.017 kb on - strand, within Pf6N2E2_1055 at 1230.017 kb on - strand, within Pf6N2E2_1055 at 1230.017 kb on - strand, within Pf6N2E2_1055 at 1230.254 kb on + strand, within Pf6N2E2_1055 at 1230.255 kb on - strand, within Pf6N2E2_1055 at 1230.255 kb on - strand, within Pf6N2E2_1055 at 1230.255 kb on - strand, within Pf6N2E2_1055 at 1230.258 kb on + strand, within Pf6N2E2_1055 at 1230.259 kb on - strand, within Pf6N2E2_1055 at 1230.259 kb on - strand, within Pf6N2E2_1055 at 1230.259 kb on - strand, within Pf6N2E2_1055 at 1230.259 kb on - strand, within Pf6N2E2_1055 at 1230.259 kb on - strand, within Pf6N2E2_1055 at 1230.419 kb on + strand at 1230.419 kb on + strand at 1230.420 kb on - strand at 1230.472 kb on - strand at 1230.547 kb on + strand at 1230.609 kb on + strand at 1230.609 kb on + strand at 1230.609 kb on + strand at 1230.660 kb on + strand at 1230.660 kb on + strand at 1230.660 kb on + strand at 1230.667 kb on + strand at 1230.667 kb on + strand at 1231.064 kb on + strand, within Pf6N2E2_1056 at 1231.085 kb on + strand, within Pf6N2E2_1056 at 1231.818 kb on - strand at 1231.933 kb on - strand at 1231.943 kb on - strand at 1231.987 kb on + strand at 1232.036 kb on + strand at 1232.043 kb on - strand at 1232.063 kb on - strand at 1232.121 kb on + strand at 1232.131 kb on - strand at 1232.247 kb on + strand at 1232.248 kb on - strand at 1232.588 kb on + strand, within Pf6N2E2_1057 at 1232.588 kb on + strand, within Pf6N2E2_1057 at 1232.588 kb on + strand, within Pf6N2E2_1057 at 1232.588 kb on + strand, within Pf6N2E2_1057 at 1232.588 kb on + strand, within Pf6N2E2_1057 at 1232.589 kb on - strand, within Pf6N2E2_1057 at 1232.589 kb on - strand, within Pf6N2E2_1057
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 1,229,687 - Pf6N2E2_1055 0.19 +0.4 1,229,687 - Pf6N2E2_1055 0.19 -0.5 1,229,804 + Pf6N2E2_1055 0.32 +0.0 1,229,901 + Pf6N2E2_1055 0.42 -0.4 1,229,901 + Pf6N2E2_1055 0.42 +0.8 1,229,902 - Pf6N2E2_1055 0.42 -1.4 1,229,902 - Pf6N2E2_1055 0.42 +1.6 1,229,930 + Pf6N2E2_1055 0.45 +0.6 1,229,930 + Pf6N2E2_1055 0.45 +0.2 1,229,931 - Pf6N2E2_1055 0.46 -0.5 1,229,931 - Pf6N2E2_1055 0.46 +1.8 1,229,963 - Pf6N2E2_1055 0.49 +1.3 1,229,963 - Pf6N2E2_1055 0.49 -1.0 1,230,016 + Pf6N2E2_1055 0.55 +0.7 1,230,016 + Pf6N2E2_1055 0.55 -0.8 1,230,016 + Pf6N2E2_1055 0.55 +0.3 1,230,016 + Pf6N2E2_1055 0.55 -0.1 1,230,017 - Pf6N2E2_1055 0.55 -1.2 1,230,017 - Pf6N2E2_1055 0.55 -0.6 1,230,017 - Pf6N2E2_1055 0.55 +0.5 1,230,254 + Pf6N2E2_1055 0.81 -1.2 1,230,255 - Pf6N2E2_1055 0.81 +0.6 1,230,255 - Pf6N2E2_1055 0.81 -0.5 1,230,255 - Pf6N2E2_1055 0.81 -0.7 1,230,258 + Pf6N2E2_1055 0.81 -0.2 1,230,259 - Pf6N2E2_1055 0.81 -0.0 1,230,259 - Pf6N2E2_1055 0.81 -0.6 1,230,259 - Pf6N2E2_1055 0.81 +1.6 1,230,259 - Pf6N2E2_1055 0.81 +0.9 1,230,259 - Pf6N2E2_1055 0.81 +0.2 1,230,419 + +0.3 1,230,419 + -0.4 1,230,420 - -0.8 1,230,472 - +0.1 1,230,547 + -0.7 1,230,609 + -0.4 1,230,609 + -1.4 1,230,609 + +1.7 1,230,660 + -1.0 1,230,660 + +1.5 1,230,660 + +0.1 1,230,667 + -1.1 1,230,667 + -0.9 1,231,064 + Pf6N2E2_1056 0.37 -2.5 1,231,085 + Pf6N2E2_1056 0.39 -2.0 1,231,818 - -1.9 1,231,933 - -0.8 1,231,943 - -0.1 1,231,987 + -0.1 1,232,036 + -0.1 1,232,043 - +0.1 1,232,063 - -0.6 1,232,121 + -0.8 1,232,131 - -0.2 1,232,247 + -0.2 1,232,248 - -0.2 1,232,588 + Pf6N2E2_1057 0.12 +0.1 1,232,588 + Pf6N2E2_1057 0.12 -0.7 1,232,588 + Pf6N2E2_1057 0.12 +0.6 1,232,588 + Pf6N2E2_1057 0.12 +0.1 1,232,588 + Pf6N2E2_1057 0.12 -0.4 1,232,589 - Pf6N2E2_1057 0.12 +1.5 1,232,589 - Pf6N2E2_1057 0.12 +0.4
Or see this region's nucleotide sequence