Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04811

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04810 and H281DRAFT_04811 are separated by 267 nucleotidesH281DRAFT_04811 and H281DRAFT_04812 are separated by 286 nucleotidesH281DRAFT_04812 and H281DRAFT_04813 are separated by 245 nucleotides H281DRAFT_04810: H281DRAFT_04810 - Sulfotransferase family protein, at 245,152 to 246,363 _04810 H281DRAFT_04811: H281DRAFT_04811 - hydroxymethylpyrimidine/phosphomethylpyrimidine kinase, at 246,631 to 247,491 _04811 H281DRAFT_04812: H281DRAFT_04812 - Rubredoxin, at 247,778 to 247,948 _04812 H281DRAFT_04813: H281DRAFT_04813 - hypothetical protein, at 248,194 to 249,603 _04813 Position (kb) 246 247 248Strain fitness (log2 ratio) -2 -1 0 1 2 3at 245.715 kb on - strand, within H281DRAFT_04810at 245.737 kb on - strand, within H281DRAFT_04810at 245.793 kb on + strand, within H281DRAFT_04810at 246.099 kb on - strand, within H281DRAFT_04810at 246.104 kb on + strand, within H281DRAFT_04810at 246.112 kb on + strand, within H281DRAFT_04810at 246.112 kb on + strand, within H281DRAFT_04810at 246.113 kb on - strand, within H281DRAFT_04810at 246.149 kb on + strand, within H281DRAFT_04810at 246.174 kb on + strand, within H281DRAFT_04810at 246.174 kb on + strand, within H281DRAFT_04810at 246.174 kb on + strand, within H281DRAFT_04810at 246.174 kb on + strand, within H281DRAFT_04810at 246.180 kb on + strand, within H281DRAFT_04810at 246.180 kb on + strand, within H281DRAFT_04810at 246.181 kb on - strand, within H281DRAFT_04810at 246.185 kb on + strand, within H281DRAFT_04810at 246.186 kb on - strand, within H281DRAFT_04810at 246.220 kb on + strand, within H281DRAFT_04810at 246.220 kb on + strand, within H281DRAFT_04810at 246.265 kb on + strandat 246.289 kb on - strandat 246.363 kb on + strandat 246.364 kb on - strandat 246.364 kb on - strandat 246.379 kb on - strandat 246.421 kb on + strandat 246.422 kb on - strandat 246.422 kb on - strandat 246.428 kb on + strandat 246.471 kb on + strandat 246.724 kb on - strand, within H281DRAFT_04811at 246.725 kb on + strand, within H281DRAFT_04811at 246.725 kb on + strand, within H281DRAFT_04811at 246.725 kb on + strand, within H281DRAFT_04811at 246.767 kb on + strand, within H281DRAFT_04811at 246.773 kb on + strand, within H281DRAFT_04811at 246.773 kb on + strand, within H281DRAFT_04811at 246.927 kb on + strand, within H281DRAFT_04811at 246.928 kb on - strand, within H281DRAFT_04811at 246.928 kb on - strand, within H281DRAFT_04811at 246.969 kb on - strand, within H281DRAFT_04811at 247.204 kb on + strand, within H281DRAFT_04811at 247.204 kb on + strand, within H281DRAFT_04811at 247.225 kb on + strand, within H281DRAFT_04811at 247.226 kb on - strandat 247.226 kb on - strand, within H281DRAFT_04811at 247.226 kb on - strand, within H281DRAFT_04811at 247.359 kb on + strand, within H281DRAFT_04811at 247.359 kb on + strand, within H281DRAFT_04811at 247.359 kb on + strand, within H281DRAFT_04811at 247.360 kb on - strand, within H281DRAFT_04811at 247.360 kb on - strand, within H281DRAFT_04811at 247.361 kb on + strand, within H281DRAFT_04811at 247.513 kb on - strandat 247.547 kb on + strandat 247.548 kb on - strandat 247.548 kb on - strandat 247.556 kb on + strandat 247.709 kb on - strandat 247.709 kb on - strandat 247.709 kb on - strandat 247.771 kb on + strandat 247.771 kb on + strandat 248.043 kb on + strandat 248.044 kb on - strandat 248.044 kb on - strandat 248.044 kb on - strandat 248.134 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM
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245,715 - H281DRAFT_04810 0.46 -0.2
245,737 - H281DRAFT_04810 0.48 -0.3
245,793 + H281DRAFT_04810 0.53 -0.6
246,099 - H281DRAFT_04810 0.78 -0.7
246,104 + H281DRAFT_04810 0.79 +0.3
246,112 + H281DRAFT_04810 0.79 -0.3
246,112 + H281DRAFT_04810 0.79 +0.2
246,113 - H281DRAFT_04810 0.79 +1.3
246,149 + H281DRAFT_04810 0.82 +0.5
246,174 + H281DRAFT_04810 0.84 +0.4
246,174 + H281DRAFT_04810 0.84 -0.2
246,174 + H281DRAFT_04810 0.84 +0.7
246,174 + H281DRAFT_04810 0.84 -0.1
246,180 + H281DRAFT_04810 0.85 -0.5
246,180 + H281DRAFT_04810 0.85 -1.0
246,181 - H281DRAFT_04810 0.85 -0.3
246,185 + H281DRAFT_04810 0.85 -1.3
246,186 - H281DRAFT_04810 0.85 +1.4
246,220 + H281DRAFT_04810 0.88 +0.9
246,220 + H281DRAFT_04810 0.88 +0.5
246,265 + +0.3
246,289 - -0.2
246,363 + -1.0
246,364 - -0.5
246,364 - -1.0
246,379 - +0.3
246,421 + +0.9
246,422 - +0.0
246,422 - -0.8
246,428 + -0.1
246,471 + +0.3
246,724 - H281DRAFT_04811 0.11 +0.0
246,725 + H281DRAFT_04811 0.11 +0.5
246,725 + H281DRAFT_04811 0.11 +0.4
246,725 + H281DRAFT_04811 0.11 +0.5
246,767 + H281DRAFT_04811 0.16 -0.9
246,773 + H281DRAFT_04811 0.16 +0.6
246,773 + H281DRAFT_04811 0.16 +0.7
246,927 + H281DRAFT_04811 0.34 -0.7
246,928 - H281DRAFT_04811 0.34 +0.3
246,928 - H281DRAFT_04811 0.34 +1.2
246,969 - H281DRAFT_04811 0.39 -0.2
247,204 + H281DRAFT_04811 0.67 +0.1
247,204 + H281DRAFT_04811 0.67 +0.7
247,225 + H281DRAFT_04811 0.69 +2.5
247,226 - +3.5
247,226 - H281DRAFT_04811 0.69 +0.1
247,226 - H281DRAFT_04811 0.69 +0.4
247,359 + H281DRAFT_04811 0.85 -0.0
247,359 + H281DRAFT_04811 0.85 +0.7
247,359 + H281DRAFT_04811 0.85 +0.4
247,360 - H281DRAFT_04811 0.85 -1.1
247,360 - H281DRAFT_04811 0.85 -1.1
247,361 + H281DRAFT_04811 0.85 +1.2
247,513 - -1.2
247,547 + +0.5
247,548 - -0.6
247,548 - -0.2
247,556 + +0.3
247,709 - +0.7
247,709 - -1.3
247,709 - +2.4
247,771 + -0.2
247,771 + +2.1
248,043 + -2.1
248,044 - -0.3
248,044 - -0.8
248,044 - +1.0
248,134 + -0.5

Or see this region's nucleotide sequence