Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04634 and H281DRAFT_04635 are separated by 60 nucleotides H281DRAFT_04635 and H281DRAFT_04636 are separated by 9 nucleotides H281DRAFT_04636 and H281DRAFT_04637 are separated by 221 nucleotides
H281DRAFT_04634: H281DRAFT_04634 - ATP-binding cassette, subfamily B, at 32,255 to 34,144
_04634
H281DRAFT_04635: H281DRAFT_04635 - Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon), at 34,205 to 34,912
_04635
H281DRAFT_04636: H281DRAFT_04636 - hypothetical protein, at 34,922 to 36,034
_04636
H281DRAFT_04637: H281DRAFT_04637 - LPS-assembly protein, at 36,256 to 38,622
_04637
Position (kb) 34
35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 34.278 kb on + strand at 34.278 kb on + strand, within H281DRAFT_04635 at 34.323 kb on + strand, within H281DRAFT_04635 at 34.932 kb on + strand at 35.108 kb on - strand, within H281DRAFT_04636 at 35.108 kb on - strand, within H281DRAFT_04636 at 35.143 kb on - strand, within H281DRAFT_04636 at 35.200 kb on - strand, within H281DRAFT_04636 at 35.202 kb on + strand, within H281DRAFT_04636 at 35.202 kb on + strand, within H281DRAFT_04636 at 35.203 kb on - strand, within H281DRAFT_04636 at 35.274 kb on + strand, within H281DRAFT_04636 at 35.274 kb on + strand, within H281DRAFT_04636 at 35.274 kb on + strand, within H281DRAFT_04636 at 35.275 kb on - strand, within H281DRAFT_04636 at 35.275 kb on - strand, within H281DRAFT_04636 at 35.275 kb on - strand, within H281DRAFT_04636 at 35.279 kb on - strand, within H281DRAFT_04636 at 35.279 kb on - strand, within H281DRAFT_04636 at 35.279 kb on - strand, within H281DRAFT_04636 at 35.289 kb on + strand, within H281DRAFT_04636 at 35.312 kb on + strand, within H281DRAFT_04636 at 35.683 kb on - strand, within H281DRAFT_04636 at 35.683 kb on - strand, within H281DRAFT_04636 at 35.883 kb on + strand, within H281DRAFT_04636 at 36.074 kb on + strand at 36.074 kb on + strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.076 kb on + strand at 36.076 kb on + strand at 36.076 kb on + strand at 36.076 kb on + strand at 36.076 kb on + strand at 36.079 kb on + strand at 36.079 kb on + strand at 36.163 kb on + strand at 36.163 kb on + strand at 36.163 kb on + strand at 36.163 kb on + strand at 36.253 kb on + strand at 36.253 kb on + strand at 36.253 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM remove 34,278 + +0.6 34,278 + H281DRAFT_04635 0.10 -2.1 34,323 + H281DRAFT_04635 0.17 -3.0 34,932 + -2.1 35,108 - H281DRAFT_04636 0.17 -1.1 35,108 - H281DRAFT_04636 0.17 -1.2 35,143 - H281DRAFT_04636 0.20 +0.7 35,200 - H281DRAFT_04636 0.25 +0.5 35,202 + H281DRAFT_04636 0.25 +0.8 35,202 + H281DRAFT_04636 0.25 +0.5 35,203 - H281DRAFT_04636 0.25 +0.4 35,274 + H281DRAFT_04636 0.32 -0.2 35,274 + H281DRAFT_04636 0.32 -0.7 35,274 + H281DRAFT_04636 0.32 -0.1 35,275 - H281DRAFT_04636 0.32 -0.1 35,275 - H281DRAFT_04636 0.32 +0.5 35,275 - H281DRAFT_04636 0.32 -0.7 35,279 - H281DRAFT_04636 0.32 -0.1 35,279 - H281DRAFT_04636 0.32 -0.2 35,279 - H281DRAFT_04636 0.32 +0.5 35,289 + H281DRAFT_04636 0.33 -0.7 35,312 + H281DRAFT_04636 0.35 +0.9 35,683 - H281DRAFT_04636 0.68 -1.2 35,683 - H281DRAFT_04636 0.68 +0.6 35,883 + H281DRAFT_04636 0.86 +0.3 36,074 + +0.5 36,074 + +0.4 36,075 - +0.0 36,075 - -0.2 36,075 - -0.5 36,075 - +1.6 36,075 - +1.1 36,076 + -0.5 36,076 + -0.6 36,076 + +0.2 36,076 + +0.3 36,076 + -1.7 36,079 + -2.2 36,079 + +0.1 36,163 + +0.5 36,163 + -0.6 36,163 + +1.3 36,163 + +0.3 36,253 + -0.2 36,253 + -0.5 36,253 + -0.0
Or see this region's nucleotide sequence