Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02382 and H281DRAFT_02383 are separated by 4 nucleotides H281DRAFT_02383 and H281DRAFT_02384 overlap by 1 nucleotides H281DRAFT_02384 and H281DRAFT_02385 are separated by 162 nucleotides
H281DRAFT_02382: H281DRAFT_02382 - amino acid/amide ABC transporter membrane protein 1, HAAT family, at 545,742 to 546,797
_02382
H281DRAFT_02383: H281DRAFT_02383 - amino acid/amide ABC transporter membrane protein 2, HAAT family /amino acid/amide ABC transporter ATP-binding protein 1, HAAT family, at 546,802 to 548,586
_02383
H281DRAFT_02384: H281DRAFT_02384 - amino acid/amide ABC transporter ATP-binding protein 2, HAAT family, at 548,586 to 549,350
_02384
H281DRAFT_02385: H281DRAFT_02385 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme, at 549,513 to 551,471
_02385
Position (kb) 546
547
548
549 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 545.856 kb on - strand at 545.891 kb on + strand, within H281DRAFT_02382 at 545.892 kb on - strand, within H281DRAFT_02382 at 545.892 kb on - strand, within H281DRAFT_02382 at 546.048 kb on + strand, within H281DRAFT_02382 at 546.048 kb on + strand, within H281DRAFT_02382 at 546.049 kb on - strand, within H281DRAFT_02382 at 546.049 kb on - strand, within H281DRAFT_02382 at 546.049 kb on - strand, within H281DRAFT_02382 at 546.049 kb on - strand, within H281DRAFT_02382 at 546.072 kb on + strand, within H281DRAFT_02382 at 546.072 kb on + strand, within H281DRAFT_02382 at 546.072 kb on + strand, within H281DRAFT_02382 at 546.073 kb on - strand, within H281DRAFT_02382 at 546.073 kb on - strand, within H281DRAFT_02382 at 546.073 kb on - strand, within H281DRAFT_02382 at 546.180 kb on + strand, within H281DRAFT_02382 at 546.393 kb on + strand, within H281DRAFT_02382 at 546.393 kb on + strand, within H281DRAFT_02382 at 546.394 kb on - strand, within H281DRAFT_02382 at 546.394 kb on - strand, within H281DRAFT_02382 at 546.394 kb on - strand, within H281DRAFT_02382 at 546.394 kb on - strand, within H281DRAFT_02382 at 546.394 kb on - strand, within H281DRAFT_02382 at 546.399 kb on + strand, within H281DRAFT_02382 at 546.560 kb on + strand, within H281DRAFT_02382 at 546.560 kb on + strand, within H281DRAFT_02382 at 546.567 kb on + strand, within H281DRAFT_02382 at 546.567 kb on + strand, within H281DRAFT_02382 at 546.568 kb on - strand, within H281DRAFT_02382 at 546.568 kb on - strand, within H281DRAFT_02382 at 546.568 kb on - strand, within H281DRAFT_02382 at 546.571 kb on - strand, within H281DRAFT_02382 at 546.637 kb on - strand, within H281DRAFT_02382 at 546.637 kb on - strand, within H281DRAFT_02382 at 546.685 kb on - strand, within H281DRAFT_02382 at 546.685 kb on - strand, within H281DRAFT_02382 at 546.685 kb on - strand, within H281DRAFT_02382 at 546.685 kb on - strand, within H281DRAFT_02382 at 546.708 kb on + strand at 546.708 kb on + strand at 546.709 kb on - strand at 546.709 kb on - strand at 546.710 kb on + strand at 546.711 kb on - strand at 546.895 kb on + strand at 546.896 kb on - strand at 546.916 kb on + strand at 546.916 kb on + strand at 546.917 kb on - strand at 546.917 kb on - strand at 546.917 kb on - strand at 547.019 kb on - strand, within H281DRAFT_02383 at 547.019 kb on - strand, within H281DRAFT_02383 at 547.030 kb on + strand, within H281DRAFT_02383 at 547.030 kb on + strand, within H281DRAFT_02383 at 547.031 kb on - strand, within H281DRAFT_02383 at 547.031 kb on - strand at 547.186 kb on + strand, within H281DRAFT_02383 at 547.186 kb on + strand, within H281DRAFT_02383 at 547.187 kb on - strand, within H281DRAFT_02383 at 547.187 kb on - strand, within H281DRAFT_02383 at 547.187 kb on - strand, within H281DRAFT_02383 at 547.187 kb on - strand, within H281DRAFT_02383 at 547.187 kb on - strand, within H281DRAFT_02383 at 547.190 kb on + strand, within H281DRAFT_02383 at 547.190 kb on + strand, within H281DRAFT_02383 at 547.190 kb on + strand, within H281DRAFT_02383 at 547.191 kb on - strand, within H281DRAFT_02383 at 547.219 kb on + strand, within H281DRAFT_02383 at 547.219 kb on + strand, within H281DRAFT_02383 at 547.285 kb on - strand, within H281DRAFT_02383 at 547.285 kb on - strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.288 kb on + strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.289 kb on - strand, within H281DRAFT_02383 at 547.291 kb on + strand, within H281DRAFT_02383 at 547.291 kb on + strand, within H281DRAFT_02383 at 547.292 kb on - strand, within H281DRAFT_02383 at 547.347 kb on + strand, within H281DRAFT_02383 at 547.416 kb on + strand, within H281DRAFT_02383 at 547.483 kb on + strand, within H281DRAFT_02383 at 547.484 kb on - strand, within H281DRAFT_02383 at 547.616 kb on + strand, within H281DRAFT_02383 at 547.616 kb on + strand, within H281DRAFT_02383 at 547.617 kb on - strand, within H281DRAFT_02383 at 547.617 kb on - strand, within H281DRAFT_02383 at 547.617 kb on - strand, within H281DRAFT_02383 at 547.617 kb on - strand, within H281DRAFT_02383 at 547.617 kb on - strand, within H281DRAFT_02383 at 547.617 kb on - strand, within H281DRAFT_02383 at 547.617 kb on - strand, within H281DRAFT_02383 at 547.617 kb on - strand, within H281DRAFT_02383 at 547.628 kb on - strand, within H281DRAFT_02383 at 547.628 kb on - strand, within H281DRAFT_02383 at 547.628 kb on - strand, within H281DRAFT_02383 at 548.114 kb on + strand, within H281DRAFT_02383 at 548.114 kb on + strand, within H281DRAFT_02383 at 548.192 kb on + strand, within H281DRAFT_02383 at 548.192 kb on + strand at 548.193 kb on - strand, within H281DRAFT_02383 at 548.193 kb on - strand, within H281DRAFT_02383 at 548.232 kb on - strand, within H281DRAFT_02383 at 548.260 kb on + strand, within H281DRAFT_02383 at 548.260 kb on + strand, within H281DRAFT_02383 at 548.260 kb on + strand, within H281DRAFT_02383 at 548.260 kb on + strand, within H281DRAFT_02383 at 548.260 kb on + strand, within H281DRAFT_02383 at 548.261 kb on - strand, within H281DRAFT_02383 at 548.261 kb on - strand, within H281DRAFT_02383 at 548.261 kb on - strand, within H281DRAFT_02383 at 548.375 kb on - strand, within H281DRAFT_02383 at 548.567 kb on - strand at 548.567 kb on - strand at 548.605 kb on + strand at 548.605 kb on + strand at 548.605 kb on + strand at 548.605 kb on + strand at 548.606 kb on - strand at 548.606 kb on - strand at 548.606 kb on - strand at 548.606 kb on - strand at 548.802 kb on + strand, within H281DRAFT_02384 at 549.113 kb on + strand, within H281DRAFT_02384 at 549.113 kb on + strand, within H281DRAFT_02384 at 549.114 kb on - strand, within H281DRAFT_02384 at 549.114 kb on - strand, within H281DRAFT_02384 at 549.237 kb on + strand, within H281DRAFT_02384 at 549.237 kb on + strand, within H281DRAFT_02384 at 549.238 kb on - strand, within H281DRAFT_02384 at 549.243 kb on + strand, within H281DRAFT_02384 at 549.244 kb on - strand, within H281DRAFT_02384 at 549.244 kb on - strand, within H281DRAFT_02384 at 549.244 kb on - strand, within H281DRAFT_02384 at 549.250 kb on + strand, within H281DRAFT_02384 at 549.250 kb on + strand, within H281DRAFT_02384 at 549.251 kb on - strand, within H281DRAFT_02384 at 549.266 kb on - strand, within H281DRAFT_02384 at 549.266 kb on - strand, within H281DRAFT_02384 at 549.266 kb on - strand, within H281DRAFT_02384 at 549.321 kb on + strand at 549.321 kb on + strand at 549.321 kb on + strand at 549.321 kb on + strand at 549.348 kb on + strand at 549.348 kb on + strand at 549.349 kb on - strand at 549.349 kb on - strand at 549.372 kb on - strand at 549.406 kb on + strand at 549.407 kb on - strand at 549.451 kb on + strand at 549.451 kb on + strand at 549.452 kb on - strand at 549.452 kb on - strand at 549.452 kb on - strand at 549.453 kb on + strand at 549.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM remove 545,856 - +1.0 545,891 + H281DRAFT_02382 0.14 +1.4 545,892 - H281DRAFT_02382 0.14 +0.0 545,892 - H281DRAFT_02382 0.14 +0.7 546,048 + H281DRAFT_02382 0.29 -0.7 546,048 + H281DRAFT_02382 0.29 +0.9 546,049 - H281DRAFT_02382 0.29 +0.2 546,049 - H281DRAFT_02382 0.29 +0.4 546,049 - H281DRAFT_02382 0.29 -1.3 546,049 - H281DRAFT_02382 0.29 +0.2 546,072 + H281DRAFT_02382 0.31 +0.6 546,072 + H281DRAFT_02382 0.31 +0.6 546,072 + H281DRAFT_02382 0.31 +0.6 546,073 - H281DRAFT_02382 0.31 -0.5 546,073 - H281DRAFT_02382 0.31 +0.4 546,073 - H281DRAFT_02382 0.31 -0.1 546,180 + H281DRAFT_02382 0.41 -0.6 546,393 + H281DRAFT_02382 0.62 +0.5 546,393 + H281DRAFT_02382 0.62 -0.3 546,394 - H281DRAFT_02382 0.62 -3.6 546,394 - H281DRAFT_02382 0.62 +1.0 546,394 - H281DRAFT_02382 0.62 -0.4 546,394 - H281DRAFT_02382 0.62 -0.4 546,394 - H281DRAFT_02382 0.62 +0.4 546,399 + H281DRAFT_02382 0.62 -0.3 546,560 + H281DRAFT_02382 0.77 +1.0 546,560 + H281DRAFT_02382 0.77 +0.2 546,567 + H281DRAFT_02382 0.78 +0.1 546,567 + H281DRAFT_02382 0.78 -0.6 546,568 - H281DRAFT_02382 0.78 -0.4 546,568 - H281DRAFT_02382 0.78 +0.4 546,568 - H281DRAFT_02382 0.78 +1.2 546,571 - H281DRAFT_02382 0.79 -0.0 546,637 - H281DRAFT_02382 0.85 +0.2 546,637 - H281DRAFT_02382 0.85 -0.7 546,685 - H281DRAFT_02382 0.89 -0.8 546,685 - H281DRAFT_02382 0.89 +0.8 546,685 - H281DRAFT_02382 0.89 -0.7 546,685 - H281DRAFT_02382 0.89 +0.2 546,708 + -0.3 546,708 + +0.1 546,709 - -0.4 546,709 - +0.3 546,710 + -0.6 546,711 - +0.2 546,895 + +0.2 546,896 - +0.7 546,916 + -0.5 546,916 + -0.1 546,917 - -0.8 546,917 - -0.3 546,917 - +0.2 547,019 - H281DRAFT_02383 0.12 -0.8 547,019 - H281DRAFT_02383 0.12 +0.7 547,030 + H281DRAFT_02383 0.13 -0.5 547,030 + H281DRAFT_02383 0.13 +0.1 547,031 - H281DRAFT_02383 0.13 +0.1 547,031 - +1.0 547,186 + H281DRAFT_02383 0.22 -1.5 547,186 + H281DRAFT_02383 0.22 -1.1 547,187 - H281DRAFT_02383 0.22 -1.4 547,187 - H281DRAFT_02383 0.22 -0.6 547,187 - H281DRAFT_02383 0.22 -0.4 547,187 - H281DRAFT_02383 0.22 +0.4 547,187 - H281DRAFT_02383 0.22 +0.6 547,190 + H281DRAFT_02383 0.22 -0.6 547,190 + H281DRAFT_02383 0.22 -0.2 547,190 + H281DRAFT_02383 0.22 -0.3 547,191 - H281DRAFT_02383 0.22 -0.1 547,219 + H281DRAFT_02383 0.23 +0.9 547,219 + H281DRAFT_02383 0.23 -0.2 547,285 - H281DRAFT_02383 0.27 -0.4 547,285 - H281DRAFT_02383 0.27 +0.1 547,288 + H281DRAFT_02383 0.27 -1.8 547,288 + H281DRAFT_02383 0.27 +0.3 547,288 + H281DRAFT_02383 0.27 +0.7 547,288 + H281DRAFT_02383 0.27 -1.9 547,288 + H281DRAFT_02383 0.27 +0.3 547,288 + H281DRAFT_02383 0.27 -0.4 547,288 + H281DRAFT_02383 0.27 +0.2 547,288 + H281DRAFT_02383 0.27 -0.4 547,288 + H281DRAFT_02383 0.27 +0.5 547,289 - H281DRAFT_02383 0.27 +0.0 547,289 - H281DRAFT_02383 0.27 -0.8 547,289 - H281DRAFT_02383 0.27 +0.4 547,289 - H281DRAFT_02383 0.27 -1.3 547,289 - H281DRAFT_02383 0.27 -0.4 547,289 - H281DRAFT_02383 0.27 -2.6 547,289 - H281DRAFT_02383 0.27 +1.0 547,289 - H281DRAFT_02383 0.27 -0.3 547,289 - H281DRAFT_02383 0.27 +0.5 547,291 + H281DRAFT_02383 0.27 -0.6 547,291 + H281DRAFT_02383 0.27 -1.2 547,292 - H281DRAFT_02383 0.27 -0.3 547,347 + H281DRAFT_02383 0.31 -0.5 547,416 + H281DRAFT_02383 0.34 -0.9 547,483 + H281DRAFT_02383 0.38 -0.2 547,484 - H281DRAFT_02383 0.38 -1.0 547,616 + H281DRAFT_02383 0.46 -0.5 547,616 + H281DRAFT_02383 0.46 -1.3 547,617 - H281DRAFT_02383 0.46 -0.8 547,617 - H281DRAFT_02383 0.46 -0.6 547,617 - H281DRAFT_02383 0.46 +0.1 547,617 - H281DRAFT_02383 0.46 +0.1 547,617 - H281DRAFT_02383 0.46 -0.8 547,617 - H281DRAFT_02383 0.46 -0.2 547,617 - H281DRAFT_02383 0.46 -0.9 547,617 - H281DRAFT_02383 0.46 -0.3 547,628 - H281DRAFT_02383 0.46 +0.3 547,628 - H281DRAFT_02383 0.46 -0.7 547,628 - H281DRAFT_02383 0.46 -0.8 548,114 + H281DRAFT_02383 0.74 +0.0 548,114 + H281DRAFT_02383 0.74 -0.4 548,192 + H281DRAFT_02383 0.78 -0.2 548,192 + +2.0 548,193 - H281DRAFT_02383 0.78 +0.7 548,193 - H281DRAFT_02383 0.78 -0.2 548,232 - H281DRAFT_02383 0.80 +0.3 548,260 + H281DRAFT_02383 0.82 +0.5 548,260 + H281DRAFT_02383 0.82 -0.4 548,260 + H281DRAFT_02383 0.82 +0.9 548,260 + H281DRAFT_02383 0.82 +1.9 548,260 + H281DRAFT_02383 0.82 +0.4 548,261 - H281DRAFT_02383 0.82 +0.4 548,261 - H281DRAFT_02383 0.82 -0.4 548,261 - H281DRAFT_02383 0.82 -0.1 548,375 - H281DRAFT_02383 0.88 -0.7 548,567 - -0.2 548,567 - +0.5 548,605 + -0.2 548,605 + -0.4 548,605 + -0.6 548,605 + +0.7 548,606 - -1.3 548,606 - +0.8 548,606 - -0.2 548,606 - +2.3 548,802 + H281DRAFT_02384 0.28 +0.6 549,113 + H281DRAFT_02384 0.69 -0.1 549,113 + H281DRAFT_02384 0.69 -0.0 549,114 - H281DRAFT_02384 0.69 +1.0 549,114 - H281DRAFT_02384 0.69 +0.5 549,237 + H281DRAFT_02384 0.85 +0.4 549,237 + H281DRAFT_02384 0.85 -0.5 549,238 - H281DRAFT_02384 0.85 -0.3 549,243 + H281DRAFT_02384 0.86 +0.5 549,244 - H281DRAFT_02384 0.86 +0.3 549,244 - H281DRAFT_02384 0.86 +0.6 549,244 - H281DRAFT_02384 0.86 -0.3 549,250 + H281DRAFT_02384 0.87 -0.2 549,250 + H281DRAFT_02384 0.87 -0.2 549,251 - H281DRAFT_02384 0.87 -1.9 549,266 - H281DRAFT_02384 0.89 -0.3 549,266 - H281DRAFT_02384 0.89 +0.3 549,266 - H281DRAFT_02384 0.89 -0.4 549,321 + -1.9 549,321 + -0.2 549,321 + +0.2 549,321 + -0.0 549,348 + -0.3 549,348 + +0.3 549,349 - -0.2 549,349 - +0.8 549,372 - +0.0 549,406 + +0.3 549,407 - +2.0 549,451 + -0.0 549,451 + +0.2 549,452 - +0.3 549,452 - -0.6 549,452 - -0.4 549,453 + -1.3 549,453 + +0.1
Or see this region's nucleotide sequence