Experiment: A22 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3427 and Ac3H11_3428 overlap by 4 nucleotides Ac3H11_3428 and Ac3H11_3429 are separated by 17 nucleotides Ac3H11_3429 and Ac3H11_3430 are separated by 144 nucleotides Ac3H11_3430 and Ac3H11_3431 overlap by 4 nucleotides
Ac3H11_3427 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 107,487 to 111,218
_3427
Ac3H11_3428 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 111,215 to 111,721
_3428
Ac3H11_3429 - Extracellular ligand-binding receptor, at 111,739 to 112,896
_3429
Ac3H11_3430 - ABC-type branched-chain amino acid transport systems, ATPase component, at 113,041 to 113,790
_3430
Ac3H11_3431 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 113,787 to 114,569
_3431
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 111.054 kb on + strand at 111.054 kb on + strand at 111.054 kb on + strand at 111.055 kb on - strand at 111.317 kb on + strand, within Ac3H11_3428 at 111.591 kb on + strand, within Ac3H11_3428 at 111.591 kb on + strand, within Ac3H11_3428 at 111.591 kb on + strand, within Ac3H11_3428 at 111.592 kb on - strand, within Ac3H11_3428 at 111.592 kb on - strand, within Ac3H11_3428 at 111.767 kb on + strand at 111.767 kb on + strand at 111.767 kb on + strand at 111.767 kb on + strand at 111.768 kb on - strand at 111.768 kb on - strand at 111.768 kb on - strand at 111.768 kb on - strand at 111.815 kb on + strand at 111.816 kb on - strand at 111.816 kb on - strand at 111.938 kb on + strand, within Ac3H11_3429 at 111.938 kb on + strand, within Ac3H11_3429 at 111.939 kb on - strand, within Ac3H11_3429 at 111.939 kb on - strand, within Ac3H11_3429 at 111.939 kb on - strand, within Ac3H11_3429 at 111.956 kb on + strand, within Ac3H11_3429 at 111.956 kb on + strand, within Ac3H11_3429 at 111.956 kb on + strand, within Ac3H11_3429 at 111.956 kb on + strand, within Ac3H11_3429 at 111.957 kb on - strand, within Ac3H11_3429 at 111.957 kb on - strand, within Ac3H11_3429 at 111.998 kb on + strand, within Ac3H11_3429 at 112.111 kb on - strand, within Ac3H11_3429 at 112.127 kb on + strand, within Ac3H11_3429 at 112.127 kb on + strand, within Ac3H11_3429 at 112.431 kb on - strand, within Ac3H11_3429 at 112.431 kb on - strand, within Ac3H11_3429 at 112.565 kb on + strand, within Ac3H11_3429 at 112.565 kb on + strand, within Ac3H11_3429 at 112.566 kb on - strand, within Ac3H11_3429 at 112.566 kb on - strand, within Ac3H11_3429 at 112.566 kb on - strand, within Ac3H11_3429 at 112.730 kb on + strand, within Ac3H11_3429 at 112.730 kb on + strand, within Ac3H11_3429 at 112.730 kb on + strand, within Ac3H11_3429 at 112.730 kb on + strand, within Ac3H11_3429 at 112.730 kb on + strand, within Ac3H11_3429 at 112.731 kb on - strand, within Ac3H11_3429 at 112.781 kb on - strand at 112.781 kb on - strand at 112.781 kb on - strand at 112.781 kb on - strand at 112.786 kb on + strand at 112.786 kb on + strand at 112.787 kb on - strand at 112.913 kb on + strand at 112.924 kb on - strand at 112.924 kb on - strand at 113.054 kb on + strand at 113.054 kb on + strand at 113.132 kb on + strand, within Ac3H11_3430 at 113.132 kb on + strand, within Ac3H11_3430 at 113.133 kb on - strand, within Ac3H11_3430 at 113.133 kb on - strand, within Ac3H11_3430 at 113.705 kb on + strand, within Ac3H11_3430 at 113.705 kb on + strand, within Ac3H11_3430 at 113.706 kb on - strand, within Ac3H11_3430 at 113.726 kb on + strand at 113.766 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.025 mg/ml remove 111,054 + -0.4 111,054 + -1.3 111,054 + +0.2 111,055 - -0.2 111,317 + Ac3H11_3428 0.20 +1.1 111,591 + Ac3H11_3428 0.74 +0.9 111,591 + Ac3H11_3428 0.74 +0.9 111,591 + Ac3H11_3428 0.74 -1.9 111,592 - Ac3H11_3428 0.74 +0.2 111,592 - Ac3H11_3428 0.74 -1.1 111,767 + +0.4 111,767 + -0.5 111,767 + -0.1 111,767 + -1.2 111,768 - -1.6 111,768 - -1.6 111,768 - +0.8 111,768 - -0.2 111,815 + +0.1 111,816 - -2.4 111,816 - -0.1 111,938 + Ac3H11_3429 0.17 +0.2 111,938 + Ac3H11_3429 0.17 -0.2 111,939 - Ac3H11_3429 0.17 +0.6 111,939 - Ac3H11_3429 0.17 +0.9 111,939 - Ac3H11_3429 0.17 +2.0 111,956 + Ac3H11_3429 0.19 -0.0 111,956 + Ac3H11_3429 0.19 -0.9 111,956 + Ac3H11_3429 0.19 -1.0 111,956 + Ac3H11_3429 0.19 -0.3 111,957 - Ac3H11_3429 0.19 -0.2 111,957 - Ac3H11_3429 0.19 -0.2 111,998 + Ac3H11_3429 0.22 -1.0 112,111 - Ac3H11_3429 0.32 -0.2 112,127 + Ac3H11_3429 0.34 +0.9 112,127 + Ac3H11_3429 0.34 -1.6 112,431 - Ac3H11_3429 0.60 -0.7 112,431 - Ac3H11_3429 0.60 -0.5 112,565 + Ac3H11_3429 0.71 -1.4 112,565 + Ac3H11_3429 0.71 +0.4 112,566 - Ac3H11_3429 0.71 +0.7 112,566 - Ac3H11_3429 0.71 +0.7 112,566 - Ac3H11_3429 0.71 -0.8 112,730 + Ac3H11_3429 0.86 -0.4 112,730 + Ac3H11_3429 0.86 -0.1 112,730 + Ac3H11_3429 0.86 +0.7 112,730 + Ac3H11_3429 0.86 -1.0 112,730 + Ac3H11_3429 0.86 +0.4 112,731 - Ac3H11_3429 0.86 +0.7 112,781 - +0.4 112,781 - -0.4 112,781 - +0.0 112,781 - -1.1 112,786 + +0.9 112,786 + +0.0 112,787 - -0.6 112,913 + +0.0 112,924 - +0.7 112,924 - -0.9 113,054 + +0.4 113,054 + +0.7 113,132 + Ac3H11_3430 0.12 -0.1 113,132 + Ac3H11_3430 0.12 -0.1 113,133 - Ac3H11_3430 0.12 -0.2 113,133 - Ac3H11_3430 0.12 -0.3 113,705 + Ac3H11_3430 0.89 -0.5 113,705 + Ac3H11_3430 0.89 -1.8 113,706 - Ac3H11_3430 0.89 +0.3 113,726 + -1.3 113,766 + +0.3
Or see this region's nucleotide sequence