Experiment: A22 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1813 and Ac3H11_1814 are separated by 66 nucleotides Ac3H11_1814 and Ac3H11_1815 overlap by 17 nucleotides Ac3H11_1815 and Ac3H11_1816 are separated by 28 nucleotides
Ac3H11_1813 - CRISPR-associated protein Cas1, at 18,717 to 19,724
_1813
Ac3H11_1814 - low-specificity D-threonine aldolase, at 19,791 to 20,960
_1814
Ac3H11_1815 - Phosphoglycerate mutase (EC 5.4.2.1), at 20,944 to 21,585
_1815
Ac3H11_1816 - Probable lipoprotein transmembrane, at 21,614 to 22,228
_1816
Position (kb)
19
20
21 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 19.018 kb on + strand, within Ac3H11_1813 at 19.019 kb on - strand, within Ac3H11_1813 at 19.019 kb on - strand, within Ac3H11_1813 at 19.019 kb on - strand, within Ac3H11_1813 at 19.091 kb on - strand, within Ac3H11_1813 at 19.110 kb on + strand, within Ac3H11_1813 at 19.201 kb on - strand, within Ac3H11_1813 at 19.201 kb on - strand, within Ac3H11_1813 at 19.260 kb on + strand, within Ac3H11_1813 at 19.260 kb on + strand, within Ac3H11_1813 at 19.261 kb on - strand, within Ac3H11_1813 at 19.300 kb on - strand, within Ac3H11_1813 at 19.300 kb on - strand, within Ac3H11_1813 at 19.363 kb on + strand, within Ac3H11_1813 at 19.363 kb on + strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.364 kb on - strand, within Ac3H11_1813 at 19.379 kb on - strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.511 kb on + strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.512 kb on - strand, within Ac3H11_1813 at 19.567 kb on + strand, within Ac3H11_1813 at 19.568 kb on - strand, within Ac3H11_1813 at 19.568 kb on - strand, within Ac3H11_1813 at 19.568 kb on - strand, within Ac3H11_1813 at 19.580 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.597 kb on + strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.598 kb on - strand, within Ac3H11_1813 at 19.602 kb on + strand, within Ac3H11_1813 at 19.642 kb on + strand at 19.642 kb on + strand at 19.643 kb on - strand at 19.643 kb on - strand at 19.643 kb on - strand at 19.645 kb on + strand at 19.645 kb on + strand at 19.645 kb on + strand at 19.645 kb on + strand at 19.645 kb on + strand at 19.646 kb on - strand at 19.681 kb on - strand at 19.747 kb on - strand at 19.776 kb on + strand at 19.777 kb on - strand at 19.777 kb on - strand at 19.966 kb on + strand, within Ac3H11_1814 at 19.999 kb on + strand, within Ac3H11_1814 at 20.396 kb on - strand, within Ac3H11_1814 at 20.396 kb on - strand, within Ac3H11_1814 at 21.108 kb on - strand, within Ac3H11_1815 at 21.239 kb on + strand, within Ac3H11_1815 at 21.239 kb on + strand, within Ac3H11_1815 at 21.239 kb on + strand, within Ac3H11_1815 at 21.240 kb on - strand, within Ac3H11_1815 at 21.240 kb on - strand, within Ac3H11_1815 at 21.240 kb on - strand, within Ac3H11_1815 at 21.425 kb on + strand, within Ac3H11_1815 at 21.425 kb on + strand, within Ac3H11_1815 at 21.425 kb on + strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.426 kb on - strand, within Ac3H11_1815 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.708 kb on + strand, within Ac3H11_1816 at 21.709 kb on - strand, within Ac3H11_1816 at 21.759 kb on + strand, within Ac3H11_1816 at 21.760 kb on - strand, within Ac3H11_1816 at 21.760 kb on - strand, within Ac3H11_1816 at 21.903 kb on + strand, within Ac3H11_1816 at 21.903 kb on + strand at 21.903 kb on + strand, within Ac3H11_1816 at 21.904 kb on - strand, within Ac3H11_1816 at 21.912 kb on + strand, within Ac3H11_1816 at 21.930 kb on + strand, within Ac3H11_1816 at 21.930 kb on + strand, within Ac3H11_1816 at 21.931 kb on - strand, within Ac3H11_1816 at 21.931 kb on - strand, within Ac3H11_1816
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.025 mg/ml remove 19,018 + Ac3H11_1813 0.30 +1.1 19,019 - Ac3H11_1813 0.30 +0.9 19,019 - Ac3H11_1813 0.30 -1.3 19,019 - Ac3H11_1813 0.30 -0.3 19,091 - Ac3H11_1813 0.37 -0.4 19,110 + Ac3H11_1813 0.39 -0.7 19,201 - Ac3H11_1813 0.48 -0.7 19,201 - Ac3H11_1813 0.48 +0.4 19,260 + Ac3H11_1813 0.54 +0.1 19,260 + Ac3H11_1813 0.54 -0.5 19,261 - Ac3H11_1813 0.54 -1.3 19,300 - Ac3H11_1813 0.58 -1.1 19,300 - Ac3H11_1813 0.58 -0.7 19,363 + Ac3H11_1813 0.64 +0.4 19,363 + Ac3H11_1813 0.64 -0.6 19,364 - Ac3H11_1813 0.64 -0.1 19,364 - Ac3H11_1813 0.64 -0.5 19,364 - Ac3H11_1813 0.64 -0.9 19,364 - Ac3H11_1813 0.64 +0.1 19,364 - Ac3H11_1813 0.64 -2.0 19,379 - Ac3H11_1813 0.66 +0.6 19,511 + Ac3H11_1813 0.79 +0.5 19,511 + Ac3H11_1813 0.79 -0.1 19,511 + Ac3H11_1813 0.79 +0.5 19,511 + Ac3H11_1813 0.79 +0.1 19,511 + Ac3H11_1813 0.79 -0.4 19,511 + Ac3H11_1813 0.79 -0.5 19,511 + Ac3H11_1813 0.79 +0.2 19,511 + Ac3H11_1813 0.79 -0.7 19,512 - Ac3H11_1813 0.79 +0.8 19,512 - Ac3H11_1813 0.79 +0.6 19,512 - Ac3H11_1813 0.79 -0.4 19,512 - Ac3H11_1813 0.79 -0.1 19,512 - Ac3H11_1813 0.79 +0.0 19,512 - Ac3H11_1813 0.79 -0.4 19,512 - Ac3H11_1813 0.79 +0.2 19,512 - Ac3H11_1813 0.79 -1.5 19,512 - Ac3H11_1813 0.79 +0.1 19,512 - Ac3H11_1813 0.79 +0.5 19,567 + Ac3H11_1813 0.84 -0.9 19,568 - Ac3H11_1813 0.84 +1.1 19,568 - Ac3H11_1813 0.84 +0.7 19,568 - Ac3H11_1813 0.84 +1.1 19,580 + Ac3H11_1813 0.86 -0.4 19,597 + Ac3H11_1813 0.87 +0.5 19,597 + Ac3H11_1813 0.87 -2.9 19,597 + Ac3H11_1813 0.87 -1.2 19,597 + Ac3H11_1813 0.87 -0.3 19,597 + Ac3H11_1813 0.87 -0.6 19,597 + Ac3H11_1813 0.87 +0.3 19,598 - Ac3H11_1813 0.87 -0.9 19,598 - Ac3H11_1813 0.87 -0.3 19,598 - Ac3H11_1813 0.87 -0.3 19,598 - Ac3H11_1813 0.87 +0.5 19,598 - Ac3H11_1813 0.87 -0.7 19,598 - Ac3H11_1813 0.87 +0.4 19,598 - Ac3H11_1813 0.87 -0.3 19,598 - Ac3H11_1813 0.87 +0.4 19,598 - Ac3H11_1813 0.87 +0.9 19,598 - Ac3H11_1813 0.87 -0.0 19,598 - Ac3H11_1813 0.87 +0.2 19,602 + Ac3H11_1813 0.88 +1.5 19,642 + -0.3 19,642 + +1.3 19,643 - -0.4 19,643 - +0.4 19,643 - -0.3 19,645 + -0.7 19,645 + -0.2 19,645 + +0.3 19,645 + +0.9 19,645 + -1.4 19,646 - +0.3 19,681 - -0.1 19,747 - +0.2 19,776 + -1.0 19,777 - -0.4 19,777 - -0.7 19,966 + Ac3H11_1814 0.15 +1.2 19,999 + Ac3H11_1814 0.18 +0.7 20,396 - Ac3H11_1814 0.52 +0.4 20,396 - Ac3H11_1814 0.52 +0.9 21,108 - Ac3H11_1815 0.26 +1.5 21,239 + Ac3H11_1815 0.46 -1.8 21,239 + Ac3H11_1815 0.46 +0.7 21,239 + Ac3H11_1815 0.46 +0.1 21,240 - Ac3H11_1815 0.46 -0.1 21,240 - Ac3H11_1815 0.46 -0.5 21,240 - Ac3H11_1815 0.46 -0.2 21,425 + Ac3H11_1815 0.75 -0.9 21,425 + Ac3H11_1815 0.75 +0.4 21,425 + Ac3H11_1815 0.75 -0.7 21,426 - Ac3H11_1815 0.75 +2.2 21,426 - Ac3H11_1815 0.75 -0.3 21,426 - Ac3H11_1815 0.75 +0.3 21,426 - Ac3H11_1815 0.75 +0.3 21,426 - Ac3H11_1815 0.75 -0.3 21,426 - Ac3H11_1815 0.75 +1.2 21,426 - Ac3H11_1815 0.75 -0.4 21,426 - Ac3H11_1815 0.75 +1.3 21,426 - Ac3H11_1815 0.75 -0.4 21,426 - Ac3H11_1815 0.75 +0.5 21,426 - Ac3H11_1815 0.75 -1.7 21,426 - Ac3H11_1815 0.75 +0.6 21,708 + Ac3H11_1816 0.15 +0.2 21,708 + Ac3H11_1816 0.15 +1.2 21,708 + Ac3H11_1816 0.15 +0.2 21,708 + Ac3H11_1816 0.15 -0.6 21,708 + Ac3H11_1816 0.15 +0.6 21,708 + Ac3H11_1816 0.15 -0.5 21,709 - Ac3H11_1816 0.15 +0.2 21,759 + Ac3H11_1816 0.24 -2.8 21,760 - Ac3H11_1816 0.24 -1.0 21,760 - Ac3H11_1816 0.24 -0.9 21,903 + Ac3H11_1816 0.47 +0.4 21,903 + +0.9 21,903 + Ac3H11_1816 0.47 -0.7 21,904 - Ac3H11_1816 0.47 -0.8 21,912 + Ac3H11_1816 0.48 +1.3 21,930 + Ac3H11_1816 0.51 +0.4 21,930 + Ac3H11_1816 0.51 +0.6 21,931 - Ac3H11_1816 0.52 +0.7 21,931 - Ac3H11_1816 0.52 -0.4
Or see this region's nucleotide sequence