Experiment: PYE with Phosphomycin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00470 and CCNA_00471 are separated by 26 nucleotides CCNA_00471 and CCNA_00472 overlap by 35 nucleotides CCNA_00472 and CCNA_00473 are separated by 104 nucleotides CCNA_00473 and CCNA_00474 overlap by 1 nucleotides
CCNA_00470: CCNA_00470 - O-antigen polymerase, at 484,789 to 486,132
_00470
CCNA_00471: CCNA_00471 - GDP-L-fucose synthase, at 486,159 to 487,127
_00471
CCNA_00472: CCNA_00472 - GDP-mannose 4,6 dehydratase, at 487,093 to 488,169
_00472
CCNA_00473: CCNA_00473 - hypothetical protein, at 488,274 to 488,768
_00473
CCNA_00474: CCNA_00474 - DNA relaxase/conjugal transfer nickase-helicase trwC, at 488,768 to 491,578
_00474
Position (kb) 487
488
489 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 486.111 kb on + strand at 486.119 kb on - strand at 486.131 kb on + strand at 486.134 kb on - strand at 486.227 kb on + strand at 486.235 kb on - strand at 486.235 kb on - strand at 486.250 kb on - strand at 486.250 kb on - strand at 486.255 kb on + strand at 486.258 kb on - strand, within CCNA_00471 at 486.274 kb on + strand at 486.307 kb on + strand, within CCNA_00471 at 486.315 kb on + strand, within CCNA_00471 at 486.328 kb on - strand, within CCNA_00471 at 486.344 kb on + strand, within CCNA_00471 at 486.345 kb on + strand, within CCNA_00471 at 486.376 kb on + strand, within CCNA_00471 at 486.376 kb on + strand, within CCNA_00471 at 486.376 kb on + strand, within CCNA_00471 at 486.384 kb on - strand, within CCNA_00471 at 486.389 kb on - strand, within CCNA_00471 at 486.402 kb on - strand, within CCNA_00471 at 486.444 kb on + strand, within CCNA_00471 at 486.450 kb on - strand, within CCNA_00471 at 486.555 kb on - strand, within CCNA_00471 at 486.591 kb on + strand, within CCNA_00471 at 486.599 kb on - strand, within CCNA_00471 at 486.609 kb on - strand, within CCNA_00471 at 486.614 kb on - strand, within CCNA_00471 at 486.626 kb on + strand, within CCNA_00471 at 486.626 kb on + strand, within CCNA_00471 at 486.626 kb on - strand, within CCNA_00471 at 486.636 kb on + strand, within CCNA_00471 at 486.637 kb on + strand, within CCNA_00471 at 486.637 kb on + strand, within CCNA_00471 at 486.639 kb on - strand, within CCNA_00471 at 486.644 kb on - strand, within CCNA_00471 at 486.644 kb on - strand, within CCNA_00471 at 486.645 kb on - strand, within CCNA_00471 at 486.645 kb on - strand, within CCNA_00471 at 486.652 kb on + strand, within CCNA_00471 at 486.692 kb on + strand, within CCNA_00471 at 486.693 kb on + strand, within CCNA_00471 at 486.703 kb on + strand, within CCNA_00471 at 486.705 kb on + strand, within CCNA_00471 at 486.721 kb on - strand, within CCNA_00471 at 486.742 kb on + strand, within CCNA_00471 at 486.748 kb on + strand, within CCNA_00471 at 486.748 kb on + strand, within CCNA_00471 at 486.751 kb on - strand, within CCNA_00471 at 486.774 kb on - strand, within CCNA_00471 at 486.779 kb on - strand, within CCNA_00471 at 486.779 kb on - strand, within CCNA_00471 at 486.779 kb on - strand, within CCNA_00471 at 486.795 kb on + strand, within CCNA_00471 at 486.803 kb on - strand, within CCNA_00471 at 486.803 kb on - strand, within CCNA_00471 at 486.885 kb on + strand, within CCNA_00471 at 486.914 kb on - strand, within CCNA_00471 at 486.921 kb on + strand, within CCNA_00471 at 486.930 kb on + strand, within CCNA_00471 at 486.956 kb on - strand, within CCNA_00471 at 486.964 kb on - strand, within CCNA_00471 at 486.969 kb on - strand, within CCNA_00471 at 486.971 kb on - strand, within CCNA_00471 at 486.971 kb on - strand, within CCNA_00471 at 486.971 kb on - strand, within CCNA_00471 at 487.149 kb on + strand at 487.157 kb on - strand at 487.162 kb on - strand at 487.162 kb on - strand at 487.167 kb on - strand at 487.172 kb on - strand at 487.187 kb on - strand at 487.223 kb on - strand, within CCNA_00472 at 487.228 kb on - strand, within CCNA_00472 at 487.238 kb on - strand, within CCNA_00472 at 487.238 kb on - strand, within CCNA_00472 at 487.245 kb on + strand, within CCNA_00472 at 487.250 kb on + strand, within CCNA_00472 at 487.253 kb on - strand, within CCNA_00472 at 487.265 kb on + strand, within CCNA_00472 at 487.270 kb on + strand, within CCNA_00472 at 487.270 kb on + strand, within CCNA_00472 at 487.283 kb on - strand, within CCNA_00472 at 487.303 kb on + strand, within CCNA_00472 at 487.399 kb on + strand, within CCNA_00472 at 487.472 kb on + strand, within CCNA_00472 at 487.487 kb on + strand, within CCNA_00472 at 487.502 kb on + strand, within CCNA_00472 at 487.515 kb on - strand, within CCNA_00472 at 487.582 kb on + strand, within CCNA_00472 at 487.586 kb on + strand, within CCNA_00472 at 487.603 kb on - strand, within CCNA_00472 at 487.626 kb on - strand, within CCNA_00472 at 487.634 kb on - strand, within CCNA_00472 at 487.635 kb on - strand, within CCNA_00472 at 487.635 kb on - strand, within CCNA_00472 at 487.658 kb on - strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.672 kb on - strand, within CCNA_00472 at 487.672 kb on - strand, within CCNA_00472 at 487.672 kb on - strand, within CCNA_00472 at 487.672 kb on - strand, within CCNA_00472 at 487.715 kb on - strand, within CCNA_00472 at 487.729 kb on + strand, within CCNA_00472 at 487.739 kb on + strand, within CCNA_00472 at 487.749 kb on + strand, within CCNA_00472 at 487.749 kb on + strand, within CCNA_00472 at 487.799 kb on - strand, within CCNA_00472 at 487.826 kb on - strand, within CCNA_00472 at 487.862 kb on - strand, within CCNA_00472 at 487.874 kb on + strand, within CCNA_00472 at 487.881 kb on - strand, within CCNA_00472 at 487.886 kb on - strand, within CCNA_00472 at 487.888 kb on - strand, within CCNA_00472 at 487.903 kb on - strand, within CCNA_00472 at 487.932 kb on - strand, within CCNA_00472 at 487.942 kb on - strand, within CCNA_00472 at 487.944 kb on + strand, within CCNA_00472 at 487.947 kb on - strand, within CCNA_00472 at 487.952 kb on - strand, within CCNA_00472 at 487.997 kb on - strand, within CCNA_00472 at 487.997 kb on - strand, within CCNA_00472 at 488.041 kb on - strand, within CCNA_00472 at 488.041 kb on - strand, within CCNA_00472 at 488.055 kb on + strand, within CCNA_00472 at 488.055 kb on + strand, within CCNA_00472 at 488.063 kb on - strand at 488.063 kb on - strand at 488.063 kb on - strand at 488.063 kb on - strand at 488.063 kb on - strand at 488.063 kb on - strand at 488.063 kb on - strand at 488.070 kb on - strand at 488.072 kb on + strand at 488.072 kb on + strand at 488.072 kb on + strand at 488.072 kb on + strand at 488.080 kb on - strand at 488.080 kb on - strand at 488.080 kb on - strand at 488.080 kb on - strand at 488.080 kb on - strand at 488.081 kb on + strand at 488.089 kb on - strand at 488.103 kb on + strand at 488.129 kb on + strand at 488.135 kb on - strand at 488.137 kb on - strand at 488.137 kb on - strand at 488.137 kb on - strand at 488.137 kb on - strand at 488.137 kb on - strand at 488.173 kb on - strand at 488.216 kb on + strand at 488.218 kb on + strand at 488.224 kb on - strand at 488.228 kb on + strand at 488.228 kb on + strand at 488.248 kb on + strand at 488.290 kb on + strand at 488.340 kb on + strand, within CCNA_00473 at 488.369 kb on + strand, within CCNA_00473 at 488.401 kb on + strand, within CCNA_00473 at 488.401 kb on + strand, within CCNA_00473 at 488.401 kb on + strand, within CCNA_00473 at 488.409 kb on - strand, within CCNA_00473 at 488.409 kb on - strand, within CCNA_00473 at 488.409 kb on - strand, within CCNA_00473 at 488.443 kb on + strand, within CCNA_00473 at 488.468 kb on + strand, within CCNA_00473 at 488.479 kb on + strand, within CCNA_00473 at 488.487 kb on + strand, within CCNA_00473 at 488.495 kb on - strand, within CCNA_00473 at 488.502 kb on - strand, within CCNA_00473 at 488.504 kb on + strand, within CCNA_00473 at 488.519 kb on + strand, within CCNA_00473 at 488.524 kb on + strand, within CCNA_00473 at 488.527 kb on + strand, within CCNA_00473 at 488.527 kb on - strand, within CCNA_00473 at 488.532 kb on + strand, within CCNA_00473 at 488.532 kb on - strand, within CCNA_00473 at 488.546 kb on - strand, within CCNA_00473 at 488.568 kb on + strand, within CCNA_00473 at 488.594 kb on - strand, within CCNA_00473 at 488.604 kb on + strand, within CCNA_00473 at 488.609 kb on - strand, within CCNA_00473 at 488.609 kb on - strand, within CCNA_00473 at 488.612 kb on - strand, within CCNA_00473 at 488.617 kb on - strand, within CCNA_00473 at 488.617 kb on - strand, within CCNA_00473 at 488.660 kb on - strand, within CCNA_00473 at 488.706 kb on - strand, within CCNA_00473 at 488.706 kb on - strand, within CCNA_00473 at 488.768 kb on + strand at 488.768 kb on + strand at 488.768 kb on + strand at 488.768 kb on + strand at 488.768 kb on + strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.783 kb on - strand at 488.826 kb on + strand at 488.826 kb on + strand at 488.840 kb on + strand at 488.877 kb on - strand at 488.877 kb on - strand at 488.879 kb on + strand at 488.939 kb on - strand at 488.950 kb on + strand at 488.950 kb on + strand at 488.950 kb on + strand at 488.950 kb on + strand at 488.950 kb on + strand at 488.950 kb on + strand at 488.958 kb on - strand at 488.980 kb on + strand at 488.999 kb on + strand at 489.007 kb on - strand at 489.007 kb on - strand at 489.025 kb on - strand at 489.025 kb on - strand at 489.026 kb on - strand at 489.047 kb on + strand at 489.047 kb on + strand at 489.064 kb on + strand, within CCNA_00474 at 489.064 kb on + strand, within CCNA_00474 at 489.064 kb on + strand, within CCNA_00474 at 489.066 kb on - strand, within CCNA_00474 at 489.077 kb on + strand, within CCNA_00474 at 489.091 kb on - strand, within CCNA_00474 at 489.097 kb on - strand, within CCNA_00474 at 489.098 kb on + strand, within CCNA_00474 at 489.098 kb on + strand, within CCNA_00474 at 489.098 kb on + strand, within CCNA_00474 at 489.098 kb on + strand, within CCNA_00474 at 489.135 kb on + strand, within CCNA_00474 at 489.150 kb on + strand, within CCNA_00474 at 489.151 kb on - strand, within CCNA_00474
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Phosphomycin 0.005 mg/ml remove 486,111 + -2.4 486,119 - -1.5 486,131 + -1.5 486,134 - -0.6 486,227 + +0.3 486,235 - -1.2 486,235 - -1.1 486,250 - -0.8 486,250 - -0.5 486,255 + -1.4 486,258 - CCNA_00471 0.10 -2.0 486,274 + +2.3 486,307 + CCNA_00471 0.15 +0.3 486,315 + CCNA_00471 0.16 +0.4 486,328 - CCNA_00471 0.17 -1.7 486,344 + CCNA_00471 0.19 -1.2 486,345 + CCNA_00471 0.19 -1.0 486,376 + CCNA_00471 0.22 -1.6 486,376 + CCNA_00471 0.22 +0.3 486,376 + CCNA_00471 0.22 +1.0 486,384 - CCNA_00471 0.23 -0.0 486,389 - CCNA_00471 0.24 -3.6 486,402 - CCNA_00471 0.25 -3.2 486,444 + CCNA_00471 0.29 -1.8 486,450 - CCNA_00471 0.30 -0.9 486,555 - CCNA_00471 0.41 -1.7 486,591 + CCNA_00471 0.45 +1.0 486,599 - CCNA_00471 0.45 -2.2 486,609 - CCNA_00471 0.46 -2.6 486,614 - CCNA_00471 0.47 -1.5 486,626 + CCNA_00471 0.48 -1.0 486,626 + CCNA_00471 0.48 +0.7 486,626 - CCNA_00471 0.48 -2.2 486,636 + CCNA_00471 0.49 +2.0 486,637 + CCNA_00471 0.49 -1.4 486,637 + CCNA_00471 0.49 -0.8 486,639 - CCNA_00471 0.50 +0.2 486,644 - CCNA_00471 0.50 -1.5 486,644 - CCNA_00471 0.50 +0.9 486,645 - CCNA_00471 0.50 -1.4 486,645 - CCNA_00471 0.50 -1.4 486,652 + CCNA_00471 0.51 -1.8 486,692 + CCNA_00471 0.55 -2.7 486,693 + CCNA_00471 0.55 -0.6 486,703 + CCNA_00471 0.56 -0.1 486,705 + CCNA_00471 0.56 -1.5 486,721 - CCNA_00471 0.58 -1.8 486,742 + CCNA_00471 0.60 -0.5 486,748 + CCNA_00471 0.61 -2.1 486,748 + CCNA_00471 0.61 -1.8 486,751 - CCNA_00471 0.61 -0.8 486,774 - CCNA_00471 0.63 +0.2 486,779 - CCNA_00471 0.64 -2.1 486,779 - CCNA_00471 0.64 +0.5 486,779 - CCNA_00471 0.64 -2.5 486,795 + CCNA_00471 0.66 +1.0 486,803 - CCNA_00471 0.66 -2.2 486,803 - CCNA_00471 0.66 -1.4 486,885 + CCNA_00471 0.75 -0.5 486,914 - CCNA_00471 0.78 -0.4 486,921 + CCNA_00471 0.79 -1.8 486,930 + CCNA_00471 0.80 -1.5 486,956 - CCNA_00471 0.82 -0.8 486,964 - CCNA_00471 0.83 -0.1 486,969 - CCNA_00471 0.84 -0.6 486,971 - CCNA_00471 0.84 -2.1 486,971 - CCNA_00471 0.84 -1.1 486,971 - CCNA_00471 0.84 -1.9 487,149 + -0.1 487,157 - +1.1 487,162 - +1.5 487,162 - +1.5 487,167 - +1.3 487,172 - +1.4 487,187 - -2.4 487,223 - CCNA_00472 0.12 -2.7 487,228 - CCNA_00472 0.13 -0.2 487,238 - CCNA_00472 0.13 -1.4 487,238 - CCNA_00472 0.13 -1.7 487,245 + CCNA_00472 0.14 -1.4 487,250 + CCNA_00472 0.15 +1.2 487,253 - CCNA_00472 0.15 -2.1 487,265 + CCNA_00472 0.16 -1.5 487,270 + CCNA_00472 0.16 -1.6 487,270 + CCNA_00472 0.16 -1.3 487,283 - CCNA_00472 0.18 -1.5 487,303 + CCNA_00472 0.19 +0.9 487,399 + CCNA_00472 0.28 -1.8 487,472 + CCNA_00472 0.35 -0.1 487,487 + CCNA_00472 0.37 -0.4 487,502 + CCNA_00472 0.38 -0.9 487,515 - CCNA_00472 0.39 -0.9 487,582 + CCNA_00472 0.45 -0.1 487,586 + CCNA_00472 0.46 -0.8 487,603 - CCNA_00472 0.47 -2.5 487,626 - CCNA_00472 0.49 -1.0 487,634 - CCNA_00472 0.50 -2.1 487,635 - CCNA_00472 0.50 -0.5 487,635 - CCNA_00472 0.50 -1.9 487,658 - CCNA_00472 0.52 -2.1 487,664 + CCNA_00472 0.53 -1.7 487,664 + CCNA_00472 0.53 +0.4 487,664 + CCNA_00472 0.53 -0.1 487,664 + CCNA_00472 0.53 -1.8 487,664 + CCNA_00472 0.53 -0.2 487,672 - CCNA_00472 0.54 -0.2 487,672 - CCNA_00472 0.54 -2.3 487,672 - CCNA_00472 0.54 -2.4 487,672 - CCNA_00472 0.54 -1.8 487,715 - CCNA_00472 0.58 -2.5 487,729 + CCNA_00472 0.59 -2.7 487,739 + CCNA_00472 0.60 +0.9 487,749 + CCNA_00472 0.61 -1.1 487,749 + CCNA_00472 0.61 -2.7 487,799 - CCNA_00472 0.66 -2.1 487,826 - CCNA_00472 0.68 -3.2 487,862 - CCNA_00472 0.71 -1.3 487,874 + CCNA_00472 0.73 +0.7 487,881 - CCNA_00472 0.73 -1.0 487,886 - CCNA_00472 0.74 -2.2 487,888 - CCNA_00472 0.74 -1.6 487,903 - CCNA_00472 0.75 -2.1 487,932 - CCNA_00472 0.78 -1.5 487,942 - CCNA_00472 0.79 -1.0 487,944 + CCNA_00472 0.79 +0.7 487,947 - CCNA_00472 0.79 -1.2 487,952 - CCNA_00472 0.80 +0.6 487,997 - CCNA_00472 0.84 -1.4 487,997 - CCNA_00472 0.84 -0.7 488,041 - CCNA_00472 0.88 -1.8 488,041 - CCNA_00472 0.88 +1.0 488,055 + CCNA_00472 0.89 +0.7 488,055 + CCNA_00472 0.89 -1.4 488,063 - -0.8 488,063 - +0.1 488,063 - -0.5 488,063 - -2.5 488,063 - -0.1 488,063 - +1.3 488,063 - -1.3 488,070 - -0.5 488,072 + -1.9 488,072 + -0.8 488,072 + -1.3 488,072 + -2.4 488,080 - -1.3 488,080 - -1.4 488,080 - +0.9 488,080 - -1.2 488,080 - +0.1 488,081 + -0.6 488,089 - +1.7 488,103 + -1.3 488,129 + -0.1 488,135 - -0.7 488,137 - -0.1 488,137 - -3.3 488,137 - -1.2 488,137 - -2.3 488,137 - -1.3 488,173 - -0.5 488,216 + -0.7 488,218 + -0.6 488,224 - +1.3 488,228 + -1.7 488,228 + -1.2 488,248 + -0.7 488,290 + -1.8 488,340 + CCNA_00473 0.13 -1.5 488,369 + CCNA_00473 0.19 -2.0 488,401 + CCNA_00473 0.26 -1.5 488,401 + CCNA_00473 0.26 +1.5 488,401 + CCNA_00473 0.26 -0.8 488,409 - CCNA_00473 0.27 -1.1 488,409 - CCNA_00473 0.27 -0.1 488,409 - CCNA_00473 0.27 -0.6 488,443 + CCNA_00473 0.34 -0.4 488,468 + CCNA_00473 0.39 -0.3 488,479 + CCNA_00473 0.41 +0.3 488,487 + CCNA_00473 0.43 +0.4 488,495 - CCNA_00473 0.45 +0.2 488,502 - CCNA_00473 0.46 -0.9 488,504 + CCNA_00473 0.46 +1.4 488,519 + CCNA_00473 0.49 +1.4 488,524 + CCNA_00473 0.51 -0.7 488,527 + CCNA_00473 0.51 -0.1 488,527 - CCNA_00473 0.51 -1.1 488,532 + CCNA_00473 0.52 -0.8 488,532 - CCNA_00473 0.52 -1.4 488,546 - CCNA_00473 0.55 -0.4 488,568 + CCNA_00473 0.59 +0.2 488,594 - CCNA_00473 0.65 +0.3 488,604 + CCNA_00473 0.67 +1.7 488,609 - CCNA_00473 0.68 -2.1 488,609 - CCNA_00473 0.68 -0.1 488,612 - CCNA_00473 0.68 -0.5 488,617 - CCNA_00473 0.69 -0.4 488,617 - CCNA_00473 0.69 -0.5 488,660 - CCNA_00473 0.78 -0.1 488,706 - CCNA_00473 0.87 -0.1 488,706 - CCNA_00473 0.87 +0.7 488,768 + -0.1 488,768 + +1.9 488,768 + -2.0 488,768 + +2.0 488,768 + +1.0 488,776 - -1.9 488,776 - -1.4 488,776 - -0.2 488,776 - -0.9 488,776 - -0.3 488,776 - +0.2 488,776 - -0.2 488,776 - +2.2 488,776 - +0.7 488,776 - +0.3 488,783 - -1.0 488,826 + -0.1 488,826 + +1.3 488,840 + +0.3 488,877 - -0.1 488,877 - +0.3 488,879 + -0.6 488,939 - -0.5 488,950 + -1.9 488,950 + -1.5 488,950 + -0.6 488,950 + -0.3 488,950 + +2.1 488,950 + -2.2 488,958 - +0.4 488,980 + -0.0 488,999 + +0.2 489,007 - +1.4 489,007 - -2.2 489,025 - -2.2 489,025 - -1.9 489,026 - -1.4 489,047 + +0.2 489,047 + -0.9 489,064 + CCNA_00474 0.11 +0.9 489,064 + CCNA_00474 0.11 +0.2 489,064 + CCNA_00474 0.11 +0.2 489,066 - CCNA_00474 0.11 +0.6 489,077 + CCNA_00474 0.11 -2.5 489,091 - CCNA_00474 0.11 -1.4 489,097 - CCNA_00474 0.12 -0.3 489,098 + CCNA_00474 0.12 +0.4 489,098 + CCNA_00474 0.12 -0.7 489,098 + CCNA_00474 0.12 -1.1 489,098 + CCNA_00474 0.12 +0.2 489,135 + CCNA_00474 0.13 +0.1 489,150 + CCNA_00474 0.14 -1.4 489,151 - CCNA_00474 0.14 -0.4
Or see this region's nucleotide sequence