Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1007 and Ac3H11_1008 are separated by 54 nucleotides Ac3H11_1008 and Ac3H11_1009 overlap by 4 nucleotides Ac3H11_1009 and Ac3H11_1010 are separated by 95 nucleotides Ac3H11_1010 and Ac3H11_1011 overlap by 4 nucleotides
Ac3H11_1007 - Hydantoin racemase (EC 5.1.99.-), at 131,361 to 132,107
_1007
Ac3H11_1008 - Prevent host death protein, Phd antitoxin, at 132,162 to 132,386
_1008
Ac3H11_1009 - Death on curing protein, Doc toxin, at 132,383 to 132,778
_1009
Ac3H11_1010 - Endonuclease III (EC 4.2.99.18), at 132,874 to 133,518
_1010
Ac3H11_1011 - Transcriptional regulator, LysR family, at 133,515 to 134,483
_1011
Position (kb) 132
133 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 131.490 kb on + strand, within Ac3H11_1007 at 131.490 kb on + strand, within Ac3H11_1007 at 131.490 kb on + strand, within Ac3H11_1007 at 131.490 kb on + strand, within Ac3H11_1007 at 131.491 kb on - strand, within Ac3H11_1007 at 131.491 kb on - strand, within Ac3H11_1007 at 131.491 kb on - strand, within Ac3H11_1007 at 131.972 kb on + strand, within Ac3H11_1007 at 131.973 kb on - strand, within Ac3H11_1007 at 132.128 kb on - strand at 132.400 kb on + strand at 132.401 kb on - strand at 132.401 kb on - strand at 132.401 kb on - strand at 132.401 kb on - strand at 132.521 kb on + strand, within Ac3H11_1009 at 132.522 kb on - strand, within Ac3H11_1009 at 132.569 kb on + strand, within Ac3H11_1009 at 132.569 kb on + strand, within Ac3H11_1009 at 132.570 kb on - strand, within Ac3H11_1009 at 132.828 kb on + strand at 132.959 kb on + strand, within Ac3H11_1010 at 132.959 kb on + strand, within Ac3H11_1010 at 132.959 kb on + strand, within Ac3H11_1010 at 132.959 kb on + strand, within Ac3H11_1010 at 132.959 kb on + strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 133.001 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.290 kb on - strand, within Ac3H11_1010 at 133.436 kb on + strand, within Ac3H11_1010 at 133.437 kb on - strand, within Ac3H11_1010 at 133.437 kb on - strand, within Ac3H11_1010 at 133.437 kb on - strand, within Ac3H11_1010
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 131,490 + Ac3H11_1007 0.17 -0.2 131,490 + Ac3H11_1007 0.17 -0.2 131,490 + Ac3H11_1007 0.17 -0.0 131,490 + Ac3H11_1007 0.17 -0.7 131,491 - Ac3H11_1007 0.17 -1.3 131,491 - Ac3H11_1007 0.17 -0.4 131,491 - Ac3H11_1007 0.17 -0.1 131,972 + Ac3H11_1007 0.82 +0.5 131,973 - Ac3H11_1007 0.82 -0.7 132,128 - +0.3 132,400 + -0.0 132,401 - -2.4 132,401 - +0.6 132,401 - +0.8 132,401 - -1.6 132,521 + Ac3H11_1009 0.35 +1.2 132,522 - Ac3H11_1009 0.35 +0.6 132,569 + Ac3H11_1009 0.47 +0.7 132,569 + Ac3H11_1009 0.47 -0.4 132,570 - Ac3H11_1009 0.47 +0.7 132,828 + -1.8 132,959 + Ac3H11_1010 0.13 -0.4 132,959 + Ac3H11_1010 0.13 -1.7 132,959 + Ac3H11_1010 0.13 -0.4 132,959 + Ac3H11_1010 0.13 -1.0 132,959 + Ac3H11_1010 0.13 +0.5 132,960 - Ac3H11_1010 0.13 -2.4 132,960 - Ac3H11_1010 0.13 -2.1 132,960 - Ac3H11_1010 0.13 -0.9 132,960 - Ac3H11_1010 0.13 +0.6 132,960 - Ac3H11_1010 0.13 -3.4 133,001 + Ac3H11_1010 0.20 +0.2 133,289 + Ac3H11_1010 0.64 -0.4 133,289 + Ac3H11_1010 0.64 -3.4 133,289 + Ac3H11_1010 0.64 -1.4 133,289 + Ac3H11_1010 0.64 -3.9 133,289 + Ac3H11_1010 0.64 -0.7 133,290 - Ac3H11_1010 0.64 -2.4 133,436 + Ac3H11_1010 0.87 -1.3 133,437 - Ac3H11_1010 0.87 -0.5 133,437 - Ac3H11_1010 0.87 -1.9 133,437 - Ac3H11_1010 0.87 -0.0
Or see this region's nucleotide sequence